Cytological evidence of terminal deficiencies produced by the r-X1 deletion in maize

Genome ◽  
1987 ◽  
Vol 29 (5) ◽  
pp. 718-721 ◽  
Author(s):  
Bor-yaw Lin
Keyword(s):  
Key Words ◽  
Centromeric Region ◽  
Chromosome 8 ◽  
Chromosome 2 ◽  
Cytological Evidence ◽  
Chromosome Breaks ◽  
Key Words Maize ◽  
The Cross

In addition to monosomes and trisomes, the r-X1 deletion generates chromosome deficiencies of parts of an arm; these deficiencies now have been identified cytologically. Of eight japonica plants produced from the cross of r-X1/R-r as female with the Mangelsdorf's tester, five were complete and three were partial monosomes. The isolated partial monosomes had chromosome breaks in the region between the centromere and the japonica locus. Their breakpoints were at the 0.33, 0.30, and 0.25 positions, respectively, on the long arm of chromosome 8. Additionally, a liguleless plant produced from the same cross carried a partial monosome with a breakpoint at the 0.09 position on the short arm of chromosome 2. Although partial monosomes associated with other chromosome arms have not been investigated, it is evident that the action of the r-X1 deletion is not restricted to the centromeric region of maize chromosomes. Key words: maize, r-X1 deletion, terminal deficiencies, monosomes.

Localization of the 68 000-Da human neurofilament gene (NF68) using a murine cDNA probe

Genome ◽  
1988 ◽  
Vol 30 (4) ◽  
pp. 499-500 ◽  
Author(s):  
Martin J. Somerville ◽  
Donald R. McLachlan ◽  
Maire E. Percy
Keyword(s):  
Centromeric Region ◽  
Human Gene ◽  
Cdna Probe ◽  
Chromosome 8 ◽  
Chromosome 7 ◽  
Gene Localization ◽  
Chromosome 2 ◽  
Genomic Probe ◽  
Human Genomic

A recent investigation, using a human genomic probe, has indicated that the 68 000 dalton neurofilament gene (NF68) is on the short arm of chromosome 8. We have used a murine cDNA probe on 65 metaphase spreads in situ to localize the human NF68 gene to 8p21 (20/370 grains; p < 0.0001). In addition, we have found secondary hybridization sites at the centromeric region of chromosome 2 and the long arm of chromosome 7, which are putative loci for other intermediate filaments.Key words: neurofilament, human, gene localization, murine cDNA.

scholarly journals Single and joint effect of the basal region of chromosome 2 and centromeric region of chromosome 8 on morphological and fruit quality traits in tomato

Euphytica ◽  
2016 ◽  
Vol 210 (3) ◽  
pp. 327-339 ◽  
Author(s):  
Gisela Yael Green ◽  
Javier Hernán Pereira da Costa ◽  
Vladimir Cambiaso ◽  
Guillermo Raúl Pratta ◽  
Roxana Zorzoli ◽  
...  
Keyword(s):  
Fruit Quality ◽  
Centromeric Region ◽  
Joint Effect ◽  
Chromosome 8 ◽  
Basal Region ◽  
Quality Traits ◽  
Chromosome 2 ◽  
Fruit Quality Traits

scholarly journals Analysis of the Functions of Recombination-Related Genes in the Generation of Large Chromosomal Deletions by Loop-Out Recombination in Aspergillus oryzae

2012 ◽  
Vol 11 (4) ◽  
pp. 507-517 ◽  
Author(s):  
Tadashi Takahashi ◽  
Masahiro Ogawa ◽  
Yasuji Koyama
Keyword(s):  
Aspergillus Oryzae ◽  
Chromosomal Region ◽  
Centromeric Region ◽  
Chromosome 8 ◽  
Telomeric Region ◽  
Dna Double Strand Breaks ◽  
Chromosomal Structure ◽  
Content Type ◽  
Strand Breaks ◽  
Chromosomal Deletions

ABSTRACT Loop-out-type recombination is a type of intrachromosomal recombination followed by the excision of a chromosomal region. The detailed mechanism underlying this recombination and the genes involved in loop-out recombination remain unknown. In the present study, we investigated the functions of ku70 , ligD , rad52 , rad54 , and rdh54 in the construction of large chromosomal deletions via loop-out recombination and the effect of the position of the targeted chromosomal region on the efficiency of loop-out recombination in Aspergillus oryzae . The efficiency of generation of large chromosomal deletions in the near-telomeric region of chromosome 3, including the aflatoxin gene cluster, was compared with that in the near-centromeric region of chromosome 8, including the tannase gene. In the Δ ku70 and Δ ku70-rdh54 strains, only precise loop-out recombination occurred in the near-telomeric region. In contrast, in the Δ ligD , Δ ku70-rad52 , and Δ ku70-rad54 strains, unintended chromosomal deletions by illegitimate loop-out recombination occurred in the near-telomeric region. In addition, large chromosomal deletions via loop-out recombination were efficiently achieved in the near-telomeric region, but barely achieved in the near-centromeric region, in the Δ ku70 strain. Induction of DNA double-strand breaks by I-SceI endonuclease facilitated large chromosomal deletions in the near-centromeric region. These results indicate that ligD , rad52 , and rad54 play a role in the generation of large chromosomal deletions via precise loop-out-type recombination in the near-telomeric region and that loop-out recombination between distant sites is restricted in the near-centromeric region by chromosomal structure.

scholarly journals LOCALIZATION IN THE TOMATO GENOME OF DNA RESTRICTION FRAGMENTS CONTAINING SEQUENCES HOMOLOGOUS TO THE rRNA (45S), THE MAJOR CHLOROPHYLL a/b BINDING POLYPEPTIDE AND THE RIBULOSE BISPHOSPHATE CARBOXYLASE GENES

Genetics ◽  
1986 ◽  
Vol 112 (1) ◽  
pp. 93-105
Author(s):  
C E Vallejos ◽  
S D Tanksley ◽  
R Bernatzky
Keyword(s):  
Chlorophyll A ◽  
Ribosomal Rna ◽  
Chromosome 8 ◽  
Ribulose Bisphosphate Carboxylase ◽  
Chromosome 2 ◽  
Ribulose Bisphosphate ◽  
Bisphosphate Carboxylase ◽  
Restriction Fragments ◽  
Dna Restriction Fragments ◽  
Dna Restriction

ABSTRACT DNA restriction fragments containing sequences homologous to the ribosomal RNA (45s), the major chlorophyll a/b binding polypeptide (CAB) and the small subunit of ribulose bisphosphate carboxylase (RBCS) genes have been localized and mapped in the tomato nuclear genome by linkage analysis. Ribosomal RNA genes map to a single locus, R45s, which resides in a terminal position on the short arm of chromosome 2 and corresponds to the Nucleolar Organizer Region. The size of the 45s repeating unit is estimated to be approximately 9 kb in Lycopersicon esculentum and 11 kb in Lycopersicon pennellii. Five loci were found to contain CAB sequences. Two of the loci, Cab-1 (chromosome 2) and Cab-3 (chromosome 8), together accounted for more than 80% of the hybridization signal. These loci contain more than one CAB structural gene. The other three loci, Cab-2 (chromosome 8), Cab-4 (chromosome 7) and Cab-5 (chromosome 12), each account for &lt;10% of the total signal and may contain only a single copy of the CAB structural sequence. Three loci were found to contain RBCS sequences. Rbcs-2 (chromosome 3) and Rbcs-3 (chromosome 2) were responsible for &gt;80% of the signal, with the remainder being associated with Rbcs-1 (chromosome 2). Rbcs-2 and Rbcs-3 may contain more than one copy of the gene.

scholarly journals Application of micronutrients and insurance herbicides in corn growing technologies

2019 ◽  
Vol 2019 (2) ◽  
pp. 25-35
Author(s):  
O.Y. Lokot ◽  
E.P. Tymoshenko ◽  
M.M. Selendy
Keyword(s):  
Key Words ◽  
Economic Efficiency ◽  
Mineral Nutrition ◽  
Mineral Fertilizers ◽  
Left Bank ◽  
Early Maturing ◽  
Key Words Maize

Studies have found that growing maize for grain on light low-humus soils of the left-Bank Polesie of Ukraine wih the use of microelements on the increased background of mineral nutrition provided the best indicators of economic efficiency. It is recommended to sow early- maturing (80 %) and mid-maturing- (20 %) hybrids with FAO 150-250 on the background of mineral fertilizers N120P60K60, applying herbicide MaizeTer power in the 3-5-leaf phase of a crop and fulfil out-off-root spraying crops with complex preparations “Rosaliq Aquatic” or ” Germ corn “. Key words: maize, insured herbicides, micro-fertilizers, early-maturing, mid-maturing, hybrids.

Generation of a variant t(2;8) translocation of Burkitt's lymphoma by site-specific recombination via the kappa light-chain joining signals

1987 ◽  
Vol 7 (6) ◽  
pp. 2037-2045
Author(s):  
P Hartl ◽  
M Lipp
Keyword(s):  
Light Chain ◽  
Half Life ◽  
Early Stage ◽  
Burkitt’S Lymphoma ◽  
Chromosome 8 ◽  
Burkitt's Lymphoma ◽  
Kappa Light Chain ◽  
Chromosome 2 ◽  
B Cell Differentiation ◽  
Myc Gene

We analyzed the Burkitt's lymphoma line BL64 in which a reciprocal translocation joins the immunoglobulin kappa light-chain locus on chromosome 2 to the c-myc gene on chromosome 8. The breakpoints on the two marker chromosomes 8+ and 2p- occurred 5' of the Js segment within the conserved nonamer and heptamer recombination sequences. Both signals were detected directly adjacent to the breakpoints in sequences of chromosome 8 suggesting that the translocation in BL64 was catalyzed by enzymes normally involved in V-J recombination. The distance between the c-myc gene and the breakpoint in J kappa amounts to at least 90 kilobases on the DNA level. In one allele of the c-myc gene somatic mutations were found in the promoter-leader region. This allele is transcribed and is supposed to be involved in the translocation. The half-life of the c-myc-specific mRNA in BL64 cells is not prolonged in comparison to the normal c-myc message. These results suggest that in Burkitt's lymphoma the translocation occurs during an early stage of B-cell differentiation and that in the variant translocations mechanisms other than a prolonged half-life, such as changes in transcriptional rates, or other posttranscriptional RNA processing contribute to the high steady-state level of c-myc RNA in the cytoplasm.

Tin-Tannin-Lignosulfonate Complex: An Improved Lignosulfonate-Based Drilling Fluid Thinner

2012 ◽  
Author(s):  
M. N. Mohamad Ibrahim ◽  
S. B. Chuah ◽  
Cheng P. Y.
Keyword(s):  
Ir Spectroscopy ◽  
Key Words ◽  
Drilling Fluid ◽  
Cross Linking ◽  
Fluid Mud ◽  
Spectroscopy Analysis ◽  
Higher Temperature ◽  
The Cross

Satu penipis lumpur gerudi yang diperbaiki iaitu kompleks stanum–tanin–lignosulfonat, telah dihasilkan dengan melakukan pengubahsuaian terhadap penipis komersil iaitu lignosulfonat. Tindak balas yang terlibat termasuk tindak balas rangkai silang antara asid lignosulfonat dengan tanin pada suhu 105°C dengan kehadiran formaldehid yang berfungsi sebagai pemula tindak balas tersebut. Campuran tersebut selanjutnya dikelatkan dengan ion stanum. Tindak balas pengubahsuaian tersebut ditentusahkan menerusi analisis spektroskopi inframerah (IR) dan prestasinya sebagai agen penipis berpolimer bagi lumpur bentonit akueus dinilai. Prestasi reologi penipis terubahsuai tersebut kemudiannya dibandingkan dengan lignosulfonat. Keputusan menunjukkan bahawa penipis terubahsuai mempunyai prestasi penipisan yang lebih baik berbanding lignosulfonat pada suhu tinggi dan juga ketahanan yang lebih tinggi terhadap pencemaran garam. Kata kunci: lignosulfonat; bendalir/lumpur gerudi;ion stanum; tannin; prestasi reologi An improved drilling fluid thinner, tin–tannin–lignosulfonate (TTLS) complex; was formulated by imposing slight modifications to a commercially available thinner, lignosulfonate. The reactions involved include reaction of a lignosulfonate acid with tannin at 105°C in the presence of formaldehyde to initiate the cross-linking reaction. The mixture was further chelating with stannous ions. The modifications reactions were verified by an infrared (IR) spectroscopy analysis and its performances as a polymeric thinning agent for aqueous bentonite mud were evaluated. The rheological performances of the modified thinner were then compared with lignosulfonate. The modified thinner shows better thinning performance at higher temperature and indicates more tolerance to salt contamination compared to lignosulfonate. Key words: lignosulfonate; drilling fluid/mud; thinner; stannousion; tannin; rheological perfomances

scholarly journals Investigation on the Occurrence of Ochratoxin A in Maize in Bangladesh

1970 ◽  
Vol 43 (4) ◽  
pp. 495-500 ◽  
Author(s):  
M Dawlatana ◽  
S Shahida ◽  
Matiur Rahim ◽  
M Tariqul Hassan
Keyword(s):  
Key Words ◽  
Incidence Rate ◽  
Ochratoxin A ◽  
Contamination Level ◽  
Contamination Rate ◽  
Key Words Maize

Maize was collected from different markets of Bangladesh for the determination of ochratoxin A. Simultaneously controlled maize samples were analyzed to assess the ochratoxin A level of market samples and that of controlled samples. A total of 120 samples were analyzed to determine ochratoxin A including 70 market samples and 50 controlled samples. Market samples were found containing a high contamination level of ochratoxin A ranged from 1 to 117 μg/kg and incidence rate was 40% whereas in the controlled samples the contamination rate was found very low ranged from 3 to 9 μg/kg and the incidence rate was found 10% only. Key words: Maize, Ochratoxin A, Occurrence, Bangladesh, HPTLC   doi: 10.3329/bjsir.v43i4.2239 Bangladesh J. Sci. Ind. Res. 43(4), 495-500, 2008

Frequency & association of maxillary ectopic canine with incisor root resorption & dental agenesis

2020 ◽  
pp. 1-11
Author(s):  
Nasreen Iqbal Nagani ◽  
Imtiaz Ahmed ◽  
Sadia Rizwan ◽  
Hana Pervez ◽  
Taskeen Khan ◽  
...  
Keyword(s):  
Oral Health ◽  
Key Words ◽  
Root Resorption ◽  
Cross Sectional Study ◽  
Health Sciences ◽  
Sectional Study ◽  
Cross Sectional ◽  
Orthodontic Patients ◽  
The Cross

Abstract Objective: To assess the frequency of maxillary ectopic canine both buccally and palatally displaced with incisor root resorption and dental agenesis. Methods: The cross-sectional study was conducted from April 8 to June 29, 2019, at the Department of Orthodontics, Dr. Ishrat Ul Ebad Khan Institute Of Oral Health Sciences, and Dow Dental College, Dow University Of Health Sciences, Karachi, and comprised orthodontic patients diagnosed with maxillary ectopic canine eruption. Root resorption and agenesis were diagnosed on panoramic films. Root resorption was further confirmed by periapical radiographs. Data was analysed using SPSS 21. Results: Of the 98 subjects, 86(87.8%) had buccally displaced canines of which 38(44.2%) were associated with root resorption, while 12(12.2%) patients had palatally displaced canines of which 10(83.3%) had root resorption (p=0.011). Overall, 30(30.6%) lateral incisors were resorbed, 7(7.1%) both central and lateral incisors, 5(5.1%) premolars, and 6(6.1%) both lateral incisors and premolars were resorbed. Agenesis was detected in 10 (10.2%) subjects (p=0.62). Conclusion: There was found to be no association of maxillary ectopic canines with incisor root resorption and dental agenesis. Key Words: Root resorption, Agenesis, Ectopic canine, Buccally displaced canine, Palatally displaced canine.

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