Precise localization of the human gene encoding cell adhesion kinase β (CAKβ/PYK2) to chromosome 8 at p21.1 by fluorescence in situ hybridization

1996 ◽  
Vol 98 (4) ◽  
pp. 508-510 ◽  
Author(s):  
J. Inazawa ◽  
Hiroko Sasaki ◽  
Kazuko Nagura ◽  
Naoki Kakazu ◽  
Tatsuo Abe ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Human Gene ◽  
Chromosome 8 ◽  
Precise Localization ◽  
Gene Encoding

588: Detection of Chromosome 8 Alterations in Paired Needle Biopsy and Prostatectomy Specimens Using Fluorescence In-Situ Hybridization

2004 ◽  
Vol 171 (4S) ◽  
pp. 156-156
Author(s):  
Chandler D. Dora ◽  
Yasushi Kondo ◽  
Fusheng X. Lan ◽  
Jeffrey M. Slezak ◽  
Erik J. Bergstralh ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Needle Biopsy ◽  
Chromosome 8

scholarly journals Localization of the human gene encoding the 13.3-kDa subunit of mitochondrial complex (UQCRB) to 8q22 by in situ hybridization

1996 ◽  
Vol 73 (4) ◽  
pp. 297-299 ◽  
Author(s):  
S. Malaney ◽  
H.H.Q. Heng ◽  
L.C. Tsui ◽  
X.M. Shi ◽  
B.H. Robinson
Keyword(s):  
In Situ Hybridization ◽  
Human Gene ◽  
Mitochondrial Complex ◽  
Gene Encoding

scholarly journals Detection of myc translocations in lymphoma cells by fluorescence in situ hybridization with yeast artificial chromosomes

Blood ◽  
1995 ◽  
Vol 85 (8) ◽  
pp. 2132-2138 ◽  
Author(s):  
ML Veronese ◽  
M Ohta ◽  
J Finan ◽  
PC Nowell ◽  
CM Croce
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Yeast Artificial Chromosome ◽  
Human Lymphocytes ◽  
Artificial Chromosome ◽  
Chromosome 8 ◽  
Metaphase Chromosomes ◽  
Artificial Chromosomes ◽  
Myc Gene

Translocations involving chromosome 8 at band q24 and one of the Ig loci on chromosomes 14q32, 22q11, and 2p11 are the hallmark of Burkitt's lymphoma (BL). It has been previously observed that the exact localization of the breakpoints at chromosome 8q24 can vary significantly from patient to patient, scattering over a distance of more than 300 kb upstream of c-myc and about 300 kb downstream of c-myc. To generate probes for fluorescence in situ hybridization (FISH) that detect most c-myc translocations, we screened a yeast artificial chromosome (YAC) library from normal human lymphocytes by colony hybridization, using three markers surrounding the c-myc gene as probes. We obtained 10 YAC clones ranging in size between 500 and 200 kb. Two nonchimeric clones were used for FISH on several BL cell lines and patient samples with different breakpoints at 8q24. Our results show that the YAC clones detected translocations scattered along approximately 200 kb in both metaphase chromosomes and interphase nuclei. The sensitivity, rapidity, and feasibility in nondividing cells render FISH an important diagnostic tool. Furthermore, the use of large DNA fragments such as YACs greatly simplifies the detection of translocations with widely scattered breakpoints such as these seen in BL.

Localization of the gene encoding the human L-glutamate transporter (GLT-1) to 11p11.2–p13 by fluorescence in situ hybridization

1996 ◽  
Vol 97 (3) ◽  
pp. 387-389 ◽  
Author(s):  
Setsuo Takai ◽  
Hideshi Kawakami ◽  
Takayasu Nakayama ◽  
Kiyomi Yamada ◽  
Shigenobu Nakamura
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Glutamate Transporter ◽  
Gene Encoding
Sign in / Sign up

Export Citation Format

Share Document