In situ digestion of Drosophila virilis polytene chromosomes by AluI and HaeIII restriction endonucleases

Genome ◽  
1987 ◽  
Vol 29 (4) ◽  
pp. 630-634 ◽  
Author(s):  
R. Mezzanotte ◽  
U. Bianchi ◽  
A. Marchi
Keyword(s):  
Base Composition ◽  
Polytene Chromosomes ◽  
Drosophila Virilis ◽  
Restriction Endonucleases ◽  
Histone Genes ◽  
Chromosomal Dna ◽  
5S Rna ◽  
5S Rna Genes ◽  
Rna Genes

Polytene chromosomes of Drosophila virilis were treated with AluI and HaeIII restriction endonucleases. Both enzymes were capable of extensively digesting chromosomal DNA, with the exception of some regions that contain repetitive DNAs. Moreover, a comparison was made between our data and the data already obtained with the same enzymes in D. melanogaster. On this basis, AluI digestion showed that the 5S RNA genes of D. virilis and D. melanogaster have different base composition, while digestion with HaeIII revealed resistance of the histone genes in D. virilis, contrary to what was previously found in D. melanogaster. Key words: restriction endonucleases, 5S RNA genes, histone genes, polytene chromosomes, Drosophila species.

Localization of the 5S RNA genes to arm 2R in polytene chromosomes of Lucilia cuprina (Diptera: Calliphoridae)

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 941-943 ◽  
Author(s):  
D. G. Bedo ◽  
G. C. Webb
Keyword(s):  
In Situ Hybridization ◽  
Ribosomal Rna ◽  
Polytene Chromosomes ◽  
Lucilia Cuprina ◽  
Chromosome 2 ◽  
5S Rna ◽  
Rna In Situ Hybridization ◽  
5S Rna Genes ◽  
Rna Genes

The 5S RNA genes of Lucilia cuprina were mapped to section 15A in the short arm of chromosome 2 by in situ hybridization to pupal trichogen polytene cells. As in most eukaryotes the 5S genes are located separately from the remaining ribosomal RNA genes.Key words: Lucilia cuprina, 5S RNA, in situ hybridization.

Localization of the 5S RNA genes in Zea mays by RNA-DNA hybridization in situ

Chromosoma ◽  
1974 ◽  
Vol 47 (4) ◽  
pp. 353-359 ◽  
Author(s):  
D. E. Wimber ◽  
Patricia A. Duffey ◽  
D. M. Steffensen ◽  
W. Prensky
Keyword(s):  
Zea Mays ◽  
Dna Hybridization ◽  
5S Rna ◽  
Hybridization In Situ ◽  
5S Rna Genes ◽  
Rna Genes

The location of 5S RNA genes in lampbrush polytene chromosomes from Drosophila

1979 ◽  
Vol 120 (2) ◽  
pp. 365-372 ◽  
Author(s):  
J.L. Bencze ◽  
K. Brasch ◽  
B.N. White
Keyword(s):  
Polytene Chromosomes ◽  
5S Rna ◽  
5S Rna Genes ◽  
Rna Genes

Hybridization of tRNAs of Drosophila melanogaster to the region of the 5S RNA genes of the polytene chromosomes

Chromosoma ◽  
1981 ◽  
Vol 82 (3) ◽  
pp. 385-397 ◽  
Author(s):  
S. Hayashi ◽  
W. R. Addison ◽  
I. C. Gillam ◽  
T. A. Grigliatti ◽  
G. M. Tener
Keyword(s):  
Drosophila Melanogaster ◽  
Polytene Chromosomes ◽  
5S Rna ◽  
5S Rna Genes ◽  
Rna Genes

Regular arrangement of nucleosomes on 5S rRNA genes in Xenopus laevis

1983 ◽  
Vol 3 (4) ◽  
pp. 720-730
Author(s):  
D Young ◽  
D Carroll
Keyword(s):  
Xenopus Laevis ◽  
Chromatin Structure ◽  
Micrococcal Nuclease ◽  
Rrna Genes ◽  
Dna Repeats ◽  
5S Rna ◽  
5S Dna ◽  
Regular Arrangement ◽  
5S Rna Genes ◽  
Rna Genes

The chromatin structure of the oocyte-type 5S RNA genes in Xenopus laevis was investigated. Blot hybridization analysis of DNA from micrococcal nuclease digests of erythrocyte nuclei showed that 5S DNA has the same average nucleosome repeat length, 192 +/- 4 base pairs, as two Xenopus satellite DNAs and bulk erythrocyte chromatin. The positions of nuclease-sensitive regions in the 5S DNA repeats of purified DNA and chromatin from erythrocytes were mapped by using an indirect end-labeling technique. Although most of the sites cleaved in purified DNA were also cleaved in chromatin, the patterns of intensities were strikingly different in the two cases. In 5S chromatin, three nuclease-sensitive regions were spaced approximately a nucleosome length apart, suggesting a single, regular arrangement of nucleosomes on most of the 5S DNA repeats. The observed nucleosome locations are discussed with respect to nucleotide sequences known to be important for expression of 5S RNA. Because the preferred locations appear to be reestablished in each repeating unit, despite spacer length heterogeneity, we suggest that the regular chromatin structure reflects the presence of a sequence-specific DNA-binding component on inactive 5S RNA genes.

Pseudogene structure in 5S RNA genes

Nature ◽  
1978 ◽  
Vol 271 (5642) ◽  
pp. 205-206 ◽  
Author(s):  
Peter Ford
Keyword(s):  
5S Rna ◽  
5S Rna Genes ◽  
Rna Genes

Localization of 5S RNA genes on chromosomes of plethodontid salamanders

Chromosoma ◽  
1978 ◽  
Vol 65 (3) ◽  
pp. 213-230 ◽  
Author(s):  
Pedro E. Le�n ◽  
James Kezer
Keyword(s):  
5S Rna ◽  
Plethodontid Salamanders ◽  
5S Rna Genes ◽  
Rna Genes

Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 1018-1021 ◽  
Author(s):  
M. Nenno ◽  
K. Schumann ◽  
W. Nagl
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Polytene Chromosomes ◽  
Seed Storage ◽  
Phaseolus Coccineus ◽  
Rna Genes

This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.Key words: plant, Leguminosae, ribosomal RNA genes, seed storage protein genes, protease.

Differential order of replication of Xenopus laevis 5S RNA genes

1986 ◽  
Vol 6 (7) ◽  
pp. 2536-2542
Author(s):  
D R Guinta ◽  
L J Korn
Keyword(s):  
Xenopus Laevis ◽  
Rna Replication ◽  
S Phase ◽  
Cold Spring Harbor ◽  
5S Rna ◽  
Expression Model ◽  
Cold Spring ◽  
5S Rna Genes ◽  
Equilibrium Centrifugation ◽  
Rna Genes

In Xenopus laevis there are two multigene families of 5S RNA genes: the oocyte-type 5S RNA genes which are expressed only in oocytes and the somatic-type 5S RNA genes which are expressed throughout development. The Xenopus 5S RNA replication-expression model of Gottesfeld and Bloomer (Cell 28:781-791, 1982) and Wormington et al. (Cold Spring Harbor Symp. Quant. Biol. 47:879-884, 1983) predicts that the somatic-type 5S RNA genes replicate earlier in the cell cycle than do the oocyte-type genes. Hence, the somatic-type 5S RNA genes have a competitive advantage in binding the transcription factor TFIIIA in somatic cells and are thereby expressed to the exclusion of the oocyte-type genes. To test the replication-expression model, we determined the order of replication of the oocyte- and somatic-type 5S RNA genes. Xenopus cells were labeled with bromodeoxyuridine, stained for DNA content, and then sorted into fractions of S phase by using a fluorescence-activated cell sorter. The newly replicated DNA containing bromodeoxyuridine was separated from the lighter, unreplicated DNA by equilibrium centrifugation and was hybridized with DNA probes specific for the oocyte- and somatic-type 5S RNA genes. In this way we found that the somatic-type 5S RNA genes replicate early in S phase, whereas the oocyte-type 5S RNA genes replicate late in S phase, demonstrating a key aspect of the replication-expression model.

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