Hybridization of tRNAs of Drosophila melanogaster to the region of the 5S RNA genes of the polytene chromosomes

Chromosoma ◽  
1981 ◽  
Vol 82 (3) ◽  
pp. 385-397 ◽  
Author(s):  
S. Hayashi ◽  
W. R. Addison ◽  
I. C. Gillam ◽  
T. A. Grigliatti ◽  
G. M. Tener
Keyword(s):  
Drosophila Melanogaster ◽  
Polytene Chromosomes ◽  
5S Rna ◽  
5S Rna Genes ◽  
Rna Genes

In situ digestion of Drosophila virilis polytene chromosomes by AluI and HaeIII restriction endonucleases

Genome ◽  
1987 ◽  
Vol 29 (4) ◽  
pp. 630-634 ◽  
Author(s):  
R. Mezzanotte ◽  
U. Bianchi ◽  
A. Marchi
Keyword(s):  
Base Composition ◽  
Polytene Chromosomes ◽  
Drosophila Virilis ◽  
Restriction Endonucleases ◽  
Histone Genes ◽  
Chromosomal Dna ◽  
5S Rna ◽  
5S Rna Genes ◽  
Rna Genes

Polytene chromosomes of Drosophila virilis were treated with AluI and HaeIII restriction endonucleases. Both enzymes were capable of extensively digesting chromosomal DNA, with the exception of some regions that contain repetitive DNAs. Moreover, a comparison was made between our data and the data already obtained with the same enzymes in D. melanogaster. On this basis, AluI digestion showed that the 5S RNA genes of D. virilis and D. melanogaster have different base composition, while digestion with HaeIII revealed resistance of the histone genes in D. virilis, contrary to what was previously found in D. melanogaster. Key words: restriction endonucleases, 5S RNA genes, histone genes, polytene chromosomes, Drosophila species.

Restriction map of 5S RNA genes of Drosophila melanogaster

Nature ◽  
1976 ◽  
Vol 263 (5574) ◽  
pp. 255-257 ◽  
Author(s):  
JAMES D. PROCUNIER ◽  
KENNETH D. TARTOF
Keyword(s):  
Drosophila Melanogaster ◽  
Restriction Map ◽  
5S Rna ◽  
5S Rna Genes ◽  
Rna Genes

The location of 5S RNA genes in lampbrush polytene chromosomes from Drosophila

1979 ◽  
Vol 120 (2) ◽  
pp. 365-372 ◽  
Author(s):  
J.L. Bencze ◽  
K. Brasch ◽  
B.N. White
Keyword(s):  
Polytene Chromosomes ◽  
5S Rna ◽  
5S Rna Genes ◽  
Rna Genes

scholarly journals GENETIC ANALYSIS OF THE 5S RNA GENES IN DROSOPHILA MELANOGASTER

Genetics ◽  
1975 ◽  
Vol 81 (3) ◽  
pp. 515-523
Author(s):  
James D Procunier ◽  
Kenneth D Tartof
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Organizer Region ◽  
Nucleolus Organizer Region ◽  
Ribosomal Rna Genes ◽  
Chromosome 2 ◽  
Wild Type ◽  
5S Rna ◽  
5S Rna Genes ◽  
Rna Genes

ABSTRACT The 5S RNA genes of Drosophila melanogaster in either an isogenic wild-type or a multiply inverted (SM1) chromosome 2 increase their multiplicity when opposite a deficiency for the 5S gene site. This is analogous to the compensation phenomenon previously described for the 18S and 28S ribosomal RNA genes of the X chromosome nucleolus organizer region. Molecular hybridization of 5S RNA to DNA containing various doses of the 56F1-9 region of chromosome 2 demonstrates that most, if not all, of the 5S genes reside in or near this region. Also, a deficiency missing approximately one-half of the wild-type number of 5S genes was isolated and genetically localized. This mutant has a phenotype like that of bobbed, a mutant known to be partially deficient in 18S and 28S ribosomal RNA genes. Finally, we report the existence of a chromosomal rearrangement which splits the second chromosome into two segments, each containing 5S DNA.

Localization of the 5S RNA genes to arm 2R in polytene chromosomes of Lucilia cuprina (Diptera: Calliphoridae)

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 941-943 ◽  
Author(s):  
D. G. Bedo ◽  
G. C. Webb
Keyword(s):  
In Situ Hybridization ◽  
Ribosomal Rna ◽  
Polytene Chromosomes ◽  
Lucilia Cuprina ◽  
Chromosome 2 ◽  
5S Rna ◽  
Rna In Situ Hybridization ◽  
5S Rna Genes ◽  
Rna Genes

The 5S RNA genes of Lucilia cuprina were mapped to section 15A in the short arm of chromosome 2 by in situ hybridization to pupal trichogen polytene cells. As in most eukaryotes the 5S genes are located separately from the remaining ribosomal RNA genes.Key words: Lucilia cuprina, 5S RNA, in situ hybridization.

Regular arrangement of nucleosomes on 5S rRNA genes in Xenopus laevis

1983 ◽  
Vol 3 (4) ◽  
pp. 720-730
Author(s):  
D Young ◽  
D Carroll
Keyword(s):  
Xenopus Laevis ◽  
Chromatin Structure ◽  
Micrococcal Nuclease ◽  
Rrna Genes ◽  
Dna Repeats ◽  
5S Rna ◽  
5S Dna ◽  
Regular Arrangement ◽  
5S Rna Genes ◽  
Rna Genes

The chromatin structure of the oocyte-type 5S RNA genes in Xenopus laevis was investigated. Blot hybridization analysis of DNA from micrococcal nuclease digests of erythrocyte nuclei showed that 5S DNA has the same average nucleosome repeat length, 192 +/- 4 base pairs, as two Xenopus satellite DNAs and bulk erythrocyte chromatin. The positions of nuclease-sensitive regions in the 5S DNA repeats of purified DNA and chromatin from erythrocytes were mapped by using an indirect end-labeling technique. Although most of the sites cleaved in purified DNA were also cleaved in chromatin, the patterns of intensities were strikingly different in the two cases. In 5S chromatin, three nuclease-sensitive regions were spaced approximately a nucleosome length apart, suggesting a single, regular arrangement of nucleosomes on most of the 5S DNA repeats. The observed nucleosome locations are discussed with respect to nucleotide sequences known to be important for expression of 5S RNA. Because the preferred locations appear to be reestablished in each repeating unit, despite spacer length heterogeneity, we suggest that the regular chromatin structure reflects the presence of a sequence-specific DNA-binding component on inactive 5S RNA genes.

Pseudogene structure in 5S RNA genes

Nature ◽  
1978 ◽  
Vol 271 (5642) ◽  
pp. 205-206 ◽  
Author(s):  
Peter Ford
Keyword(s):  
5S Rna ◽  
5S Rna Genes ◽  
Rna Genes
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