Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 1018-1021 ◽  
Author(s):  
M. Nenno ◽  
K. Schumann ◽  
W. Nagl
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Polytene Chromosomes ◽  
Seed Storage ◽  
Phaseolus Coccineus ◽  
Rna Genes

This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.Key words: plant, Leguminosae, ribosomal RNA genes, seed storage protein genes, protease.

Localization of the 5S RNA genes to arm 2R in polytene chromosomes of Lucilia cuprina (Diptera: Calliphoridae)

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 941-943 ◽  
Author(s):  
D. G. Bedo ◽  
G. C. Webb
Keyword(s):  
In Situ Hybridization ◽  
Ribosomal Rna ◽  
Polytene Chromosomes ◽  
Lucilia Cuprina ◽  
Chromosome 2 ◽  
5S Rna ◽  
Rna In Situ Hybridization ◽  
5S Rna Genes ◽  
Rna Genes

The 5S RNA genes of Lucilia cuprina were mapped to section 15A in the short arm of chromosome 2 by in situ hybridization to pupal trichogen polytene cells. As in most eukaryotes the 5S genes are located separately from the remaining ribosomal RNA genes.Key words: Lucilia cuprina, 5S RNA, in situ hybridization.

Localization of 18S+28S and 5S ribosomal RNA genes in the dog by fluorescence in situ hybridization

1997 ◽  
Vol 78 (3-4) ◽  
pp. 231-235 ◽  
Author(s):  
A. Mäkinen ◽  
C. Zijlstra ◽  
N.A. de Haan ◽  
C.H.M. MellInk ◽  
A.A. Bosma
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
5S Ribosomal Rna ◽  
Rna Genes

scholarly journals Karyotype Analysis of Three Juniperus Species using Fluorescence in situ Hybridization (FISH) with Two Ribosomal RNA Genes

CYTOLOGIA ◽  
2007 ◽  
Vol 72 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Katsuya Nagano ◽  
Hideyuki Matoba ◽  
Kouhei Yonemura ◽  
Yasushi Matsuda ◽  
Tatsurou Murata ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Karyotype Analysis ◽  
Ribosomal Rna Genes ◽  
Rna Genes

scholarly journals Detection of the Ribosomal RNA Gene in Pear (Pyrus spp.) using Fluorescence in situ Hybridization

2010 ◽  
Vol 79 (4) ◽  
pp. 335-339 ◽  
Author(s):  
Masashi Yamamoto ◽  
Shingo Terakami ◽  
Toshiya Yamamoto ◽  
Norio Takada ◽  
Tatsuya Kubo ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Ribosomal Rna Gene

Quantitative in situ hybridization of ribosomal RNA species to polytene chromosomes of Drosophila melanogaster

1977 ◽  
Vol 115 (3) ◽  
pp. 539-563 ◽  
Author(s):  
Paul Szabo ◽  
Robert Elder ◽  
Dale M. Steffensen ◽  
Olke C. Uhlenbeck
Keyword(s):  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Ribosomal Rna ◽  
Polytene Chromosomes
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