Q-banding methods using quinacrine (QFQ) and hoechst 33258 (QFH) for chromosome analysis of human lymphocyte cultures

1978 ◽  
Vol 4 (4) ◽  
pp. 937-940 ◽  
Author(s):  
C. C. Lin ◽  
Brian Biederman ◽  
Heidi Jamro
1985 ◽  
Vol 27 (5) ◽  
pp. 622-625 ◽  
Author(s):  
M. De Braekeleer ◽  
Marylinne Keushnig ◽  
C. C. Lin

A simple technique for synchronization of human lymphocyte cultures with fluorodeoxyuridine (FudR) is presented. The S-phase block induced by the FudR is released by simultaneous exposure to 5-bromodeoxyuridine (BrdU) and Hoechst 33258 or by thymidine and Hoechst 33258. This method provides a high mitotic index with high percentage of prometaphase chromosomes. This simple method is highly advantageous and easy to utilize in clinical cytogenetics.Key words: fluorodeoxyuridine, high-resolution human chromosomes, cell synchronization, banding.


1980 ◽  
Vol 2 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Henry E. Holden ◽  
Paula A. Crider ◽  
Margitta G. Wahrenburg

1979 ◽  
Vol 156 (1-2) ◽  
pp. 65-75
Author(s):  
H. Kirchner ◽  
H.H. Peter ◽  
H.M. Hirt ◽  
R. Zawatzky ◽  
H. Dalügge ◽  
...  

1982 ◽  
Vol 57 (1) ◽  
pp. 129-137
Author(s):  
MH Heulin ◽  
M Artur ◽  
J Straczek ◽  
F Belleville ◽  
P Nabet ◽  
...  

2011 ◽  
Vol 64 (4) ◽  
pp. 219-223 ◽  
Author(s):  
Irine Karapidaki ◽  
Maria T. Ekonomopoulou ◽  
Kyriaki Akritopoulou ◽  
Doxakis Anestakis ◽  
Zafiroula Iakovidou-Kritsi

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