FUSION AND DIVISION OF NUCLEI IN MULTINUCLEATED SOYBEAN PROTOPLASTS

1971 ◽  
Vol 13 (2) ◽  
pp. 347-353 ◽  
Author(s):  
R. A. Miller ◽  
O. L. Gamborg ◽  
W. A. Keller ◽  
K. N. Kao
Keyword(s):  
Cell Culture ◽  
Cell Wall ◽  
Protoplast Fusion ◽  
Nuclear Division ◽  
Nuclear Fusion ◽  
Complete Synchronization ◽  
Cellular Division ◽  
Nuclear Behaviour ◽  
Soybean Cell

Protoplasts were produced from a soybean cell culture by enzymatic removal of the cell wall. The protoplasts were fixed after various periods of culture. There was a large amount of protoplast fusion during formation. The nuclear behaviour during division was observed. Nuclear fusion prior to nuclear division was common. Almost complete synchronization of multinucleates was found. Various abnormalities were present in nuclear and cellular division which could have led to aneuploid production.

Isolation of sodium dependent variants from haploid soybean cell culture

1981 ◽  
Vol 1 (2) ◽  
pp. 48-51 ◽  
Author(s):  
Zhou Jia-Ping ◽  
E. Jill Roth ◽  
William Terzaghi ◽  
Karl G. Lark
Keyword(s):  
Cell Culture ◽  
Sodium Dependent ◽  
Soybean Cell

scholarly journals Light activates a 46-kDa MAP kinase-like protein kinase in soybean cell culture

FEBS Letters ◽  
2001 ◽  
Vol 494 (1-2) ◽  
pp. 24-29 ◽  
Author(s):  
Hiroshi Yamagata ◽  
Kazunori Saka ◽  
Tomofumi Tanaka ◽  
Yasuo Aizono
Keyword(s):  
Cell Culture ◽  
Protein Kinase ◽  
Map Kinase ◽  
Soybean Cell

Ultrastructure of fusion products from soybean cell culture and sweet clover leaf protoplasts

Planta ◽  
1976 ◽  
Vol 130 (1) ◽  
pp. 39-45 ◽  
Author(s):  
L. C. Fowke ◽  
P. J. Rennie ◽  
J. W. Kirkpatrick ◽  
F. Constabel
Keyword(s):  
Cell Culture ◽  
Sweet Clover ◽  
Fusion Products ◽  
Soybean Cell ◽  
Leaf Protoplasts

scholarly journals The Induction of Ethylene Production from Pear Cell Culture by Cell Wall Fragments

1986 ◽  
Vol 81 (3) ◽  
pp. 929-930 ◽  
Author(s):  
Cindy B. Tong ◽  
John M. Labavitch ◽  
Shang Fa Yang
Keyword(s):  
Cell Culture ◽  
Cell Wall ◽  
Ethylene Production

Host-Pathogen Interactions XXX. Characterization of Elicitors of Phytoalexin Accumulation in Soybean Released from Soybean Cell Walls by Endopolygalacturonic Acid Lyase

1986 ◽  
Vol 41 (1-2) ◽  
pp. 39-48 ◽  
Author(s):  
Keith R. Davis ◽  
Alan G. Darvill ◽  
Peter Albersheim ◽  
Anne Dell
Keyword(s):  
Cell Wall ◽  
Glycine Max ◽  
Cell Walls ◽  
Erwinia Carotovora ◽  
Citrus Pectin ◽  
Phytopathogenic Bacterium ◽  
Heat Stable ◽  
Glycine Max L ◽  
Soybean Cell

Abstract Endopolygalacturonic acid lyase, purified from the phytopathogenic bacterium, Erwinia carotovora, induces phytoalexin accumulation in soybean (Glycine max L.) cotyledons. This pectin-degrading enzyme releases heat-stable elicitors of phytoalexin accumulation from soybean cell walls, citrus pectin, and citrus sodium polypectate. The most elicitor-active molecules ob­tained by treating soybean cell walls with endopolygalacturonic acid lyase have been purified and characterized. The cell-wall-derived elicitors are α-1,4-linked oligogalacturonides with degrees of polymerization of eight to twelve residues. The molecules with the highest specific elicitor activity were identified as α-1,4 -linked deca- and undecagalacturonides that contained 4,5-unsaturated galactosyluronic acid at the nonreducing termini.

Fertilization in brown algae. I. SEM and other observations on Fucus serratus

1978 ◽  
Vol 32 (1) ◽  
pp. 45-54
Author(s):  
M.E. Callow ◽  
L.V. Evans ◽  
G.P. Bolwell ◽  
J.A. Callow
Keyword(s):  
Cell Wall ◽  
Brown Algae ◽  
Sea Urchins ◽  
Nuclear Fusion ◽  
Fluorescent Dye ◽  
White Cell ◽  
Calcofluor White ◽  
Fucus Serratus ◽  
Sperm Entry

The cell wall secreted immediately following sperm entry into an egg can be visualized by the fluorescent dye Calcofluor white. Cell wall secretion precedes nuclear fusion by 10–20 min. SEM observations of the surface of unfertilized and fertilized eggs and sperm attachment to eggs are described. These results are discussed in relation to fertilization in sea urchins and the biochemical phenomena associated with egg-sperm recognition in Fucus.

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