Ultrastructure of fusion products from soybean cell culture and sweet clover leaf protoplasts

Planta ◽  
1976 ◽  
Vol 130 (1) ◽  
pp. 39-45 ◽  
Author(s):  
L. C. Fowke ◽  
P. J. Rennie ◽  
J. W. Kirkpatrick ◽  
F. Constabel
Keyword(s):  
Cell Culture ◽  
Sweet Clover ◽  
Fusion Products ◽  
Soybean Cell ◽  
Leaf Protoplasts

Fine structure of fusion products from soybean cell culture and pea leaf protoplasts

Planta ◽  
1977 ◽  
Vol 135 (3) ◽  
pp. 257-266 ◽  
Author(s):  
L. C. Fowke ◽  
F. Constabel ◽  
O. L. Gamborg
Keyword(s):  
Cell Culture ◽  
Fine Structure ◽  
Fusion Products ◽  
Soybean Cell ◽  
Leaf Protoplasts

Isolation of sodium dependent variants from haploid soybean cell culture

1981 ◽  
Vol 1 (2) ◽  
pp. 48-51 ◽  
Author(s):  
Zhou Jia-Ping ◽  
E. Jill Roth ◽  
William Terzaghi ◽  
Karl G. Lark
Keyword(s):  
Cell Culture ◽  
Sodium Dependent ◽  
Soybean Cell

scholarly journals Light activates a 46-kDa MAP kinase-like protein kinase in soybean cell culture

FEBS Letters ◽  
2001 ◽  
Vol 494 (1-2) ◽  
pp. 24-29 ◽  
Author(s):  
Hiroshi Yamagata ◽  
Kazunori Saka ◽  
Tomofumi Tanaka ◽  
Yasuo Aizono
Keyword(s):  
Cell Culture ◽  
Protein Kinase ◽  
Map Kinase ◽  
Soybean Cell

FUSION AND DIVISION OF NUCLEI IN MULTINUCLEATED SOYBEAN PROTOPLASTS

1971 ◽  
Vol 13 (2) ◽  
pp. 347-353 ◽  
Author(s):  
R. A. Miller ◽  
O. L. Gamborg ◽  
W. A. Keller ◽  
K. N. Kao
Keyword(s):  
Cell Culture ◽  
Cell Wall ◽  
Protoplast Fusion ◽  
Nuclear Division ◽  
Nuclear Fusion ◽  
Complete Synchronization ◽  
Cellular Division ◽  
Nuclear Behaviour ◽  
Soybean Cell

Protoplasts were produced from a soybean cell culture by enzymatic removal of the cell wall. The protoplasts were fixed after various periods of culture. There was a large amount of protoplast fusion during formation. The nuclear behaviour during division was observed. Nuclear fusion prior to nuclear division was common. Almost complete synchronization of multinucleates was found. Various abnormalities were present in nuclear and cellular division which could have led to aneuploid production.

scholarly journals Effect of Amitrole on Growth and Organic Nitrogen Metabolism in Soybean Cell Culture

1979 ◽  
Vol 93 (1) ◽  
pp. 31-43 ◽  
Author(s):  
Roger S.K. Suen ◽  
Jacques A. Lenoir ◽  
Ragai K. Ibrahim
Keyword(s):  
Cell Culture ◽  
Nitrogen Metabolism ◽  
Organic Nitrogen ◽  
Soybean Cell

Callus production from photoautotrophic soybean cell culture protoplasts

1985 ◽  
Vol 4 (5) ◽  
pp. 289-292 ◽  
Author(s):  
Vijay K. Chowhury ◽  
Jack M. Widholm
Keyword(s):  
Cell Culture ◽  
Callus Production ◽  
Soybean Cell

Laser-induced fusion of mammalian cells and plant protoplasts

1987 ◽  
Vol 88 (2) ◽  
pp. 145-149
Author(s):  
R. Wiegand ◽  
G. Weber ◽  
K. Zimmermann ◽  
S. Monajembashi ◽  
J. Wolfrum ◽  
...  
Keyword(s):  
Cell Culture ◽  
B Lymphocytes ◽  
Mammalian Cells ◽  
Culture Medium ◽  
Laser Light ◽  
Plant Protoplasts ◽  
Myeloma Cells ◽  
Normal Culture ◽  
Laser Microbeam ◽  
Fusion Products

An ultraviolet-laser microbeam was shown to be suitable for inducing fusion of individually selected plant protoplasts or of B-lymphocytes with myeloma cells. The fusion took place in normal culture medium and the fusogenic condition perturbed the cells only for a fraction of a millisecond. Without manipulating the cell culture except for exposing the cells to laser light, fusion products between preselected individual pairs may be produced.

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