Localization of QTLs for seed color using recombinant inbred lines of Brassica napus in different environments

Genome ◽  
2007 ◽  
Vol 50 (9) ◽  
pp. 840-854 ◽  
Author(s):  
Fu-You Fu ◽  
Lie-Zhao Liu ◽  
You-Rong Chai ◽  
Li Chen ◽  
Tao Yang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Candidate Gene ◽  
Recombinant Inbred ◽  
Seed Color ◽  
Srap Marker ◽  
Linkage Groups ◽  
Yellow Seed ◽  
Seed Trait ◽  
Major Qtl ◽  
Selection Of

Yellow seed is one of the most important traits of Brassica napus L. Efficient selection of the yellow-seed trait is one of the most important objectives in oilseed rape breeding. Two recombinant inbred line (RIL) populations (RIL-1 and RIL-2) were analyzed for 2 years at 2 locations. Four hundred and twenty SSR, RAPD, and SRAP marker loci covering 1744 cM were mapped in 26 linkage groups of RIL-1, while 265 loci covering 1135 cM were mapped in 20 linkage groups of RIL-2. A total of 19 QTLs were detected in the 2 populations. A major QTL was detected adjacent to the same marker (EM11ME20/200) in both maps in both years. This major QTL could explain 53.71%, 39.34%, 42.42%, 30.18%, 24.86%, and 15.08% of phenotypic variation in 6 combinations (location × year × population). BLASTn analysis of the sequences of the markers flanking the major QTL revealed that the homologous region corresponding to this major QTL was anchored between genes At5g44440 and At5g49640 of Arabidopsis thaliana chromosome 5 (At C5). Based on comparative genomic analysis, the bifunctional gene TT10 is nearest to the homologue of EM11ME20/200 on At C5 and can be considered an important candidate gene for the major QTL identified here. Besides providing an effective strategy for marker-assisted selection of the yellow-seed trait in B. napus, our results also provide important clues for cloning of the candidate gene corresponding to this major QTL.

Identification of two major QTL for yellow seed color in two crosses of resynthesized Brassica napus line No. 2127-17

2010 ◽  
Vol 28 (3) ◽  
pp. 335-342 ◽  
Author(s):  
Yan Zhang ◽  
Xia Li ◽  
Wei Chen ◽  
Bin Yi ◽  
Jing Wen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Color ◽  
Yellow Seed ◽  
Resynthesized Brassica Napus ◽  
Napus Line ◽  
Major Qtl

scholarly journals Mapping and identification of yellow seed coat color genes in Brassica juncea L.

2020 ◽  
Author(s):  
Zhen Huang ◽  
Yang Wang ◽  
Hong Lu ◽  
Xiang Liu ◽  
Lu Liu ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Brassica Juncea ◽  
Seed Coat ◽  
Coat Color ◽  
Seed Color ◽  
Seed Coat Color ◽  
Flavonoid Pathway ◽  
Yellow Seed ◽  
Color Formation ◽  
Yellow Seed Coat

Abstract BackgroundYellow seed breeding is an effective method to improve the oil content in rapeseed. Yellow seed coat color formation is influenced by various factors, and no clear mechanisms are known. In this study, Bulked segregant RNA-Seq (BSR-Seq) of BC9 population of Wuqi mustard (yellow seed) and Wugong mustard (brown seed) was used to identity the candidate genes controlling the yellow seed color in Brassica juncea L.ResultsYellow seed coat color gene was mapped to chromosome A09, and differentially expressed genes (DEGs) between brown and yellow bulks enriched in the flavonoid pathway. A significant correlation between the expression of BjF3H and BjTT5 and the content of the seed coat color related indexes was identified. Two intron polymorphism (IP) markers linked to the target gene were developed around BjF3H. Therefore, BjF3H was considered as the candidate gene. The BjF3H coding sequences (CDS) of Wuqi mustard and Wugong mustard are 1071-1077bp, encoding protein of 356-358 amino acids. One amino acid change (254, F/V) was identified in the conserved domain. This mutation site was detected in four Brassica rapa (B. rapa) and six Brassica juncea (B. juncea) lines, but not in Brassica napus (B. napus).ConclusionsThe results indicated BjF3H is a candidate gene that related to yellow seed coat color formation in Brassica juncea and provided a comprehensive understanding of the yellow seed coat color mechanism.

scholarly journals Prospects for the creation of the original material for the selection of yellow seed varieties oil crops of the Brassicaceae family

2020 ◽  
Vol 27 ◽  
pp. 282-286
Author(s):  
S. G. Hablak ◽  
Y. A. Abdullaeva ◽  
L. O. Ryabovol ◽  
Y. S. Ryabovol
Keyword(s):  
Theoretical Research ◽  
Original Material ◽  
Seed Color ◽  
Breeding Programs ◽  
Yellow Seed ◽  
Oil Crops ◽  
Yellow Color ◽  
Analysis And Synthesis ◽  
Breeding Grounds ◽  
Selection Of

Aim. Study of the genetic resources of Arabidopsis for the purpose of detecting mutations with selection-valuable alleles of interest for transgenesis in cultivated oil plants of the genus. Brassicaceae by genetic engineering and biotechnology. Methods. Empirical research (observation, comparison), theoretical research (abstraction, analysis and synthesis). Results. A study of genetic collections of Arabidopsis resources (NASC and ABRC) showed that there are mutations in international collection centers (tt1-1; tt2-1; tt3-1; tt4-1; tt5-1; tt8-1 and ttg-1) with breeding-valuable alleles suitable for transgenesis of alleles in cultivated oilseeds of the genus. Brassicaceae in order to create a source material with a yellow seed color. Conclusions. The study of the potential of the largest international Arabidopsis collection centers of NASC, ABRC and SASSC on basic biological and breeding grounds allows us to identify economically valuable alleles for the implementation of breeding programs in different directions. Keywords: Arabidopsis thaliana (L.) Heynh., Brassicaceae, seeds, yellow color.

Identification of QTLs involved in pod-shatter resistance in Brassica napus L.

2012 ◽  
Vol 63 (12) ◽  
pp. 1082 ◽  
Author(s):  
Y. C. Wen ◽  
S. F. Zhang ◽  
B. Yi ◽  
J. Wen ◽  
J. P. Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Phenotypic Variation ◽  
Linkage Groups ◽  
Brassica Napus L ◽  
Dh Population ◽  
Seed Loss ◽  
Pod Shatter ◽  
Rapeseed Production ◽  
Simple Sequence ◽  
Selection Of

Seed loss caused by pod-shatter during harvesting is one of the main problems in rapeseed production worldwide. Quantitative trait loci (QTLs) for pod-shatter based on genetic mapping would help breeders develop cultivars resistant to pod-shatter. In this study, we constructed a genetic map of Brassica napus containing 107 simple sequence repeat (SSR) markers and 68 sequence-related amplified polymorphism (SRAP) markers using a doubled-haploid (DH) population of 276 lines derived from the cross H155 × Qva. This map covered 1382.8 cM with an average marker interval of 7.9 cM. In total, 13 QTLs for pod-shatter resistance were identified in this DH population at two experimental sites (in Wuhan and Zhengzhou); three of the QTLs were present at both locations. At Zhengzhou, nine QTLs, identified in linkage groups A1, A7, A8, C5, and C8, together explained 49.0% of the phenotypic variation. At Wuhan, four QTLs were mapped on the A1, A4, A7, and C8 linkage groups. These QTLs explained 38.6% of the phenotypic variation. These results may serve as a valuable basis for further molecular dissection of pod-shatter resistance in B. napus, and for development of the markers related to QTLs that may be useful for marker-assisted selection of pod-shatter resistant cultivars.

Colocalization of a partially dominant gene for yellow seed colour with a major QTL influencing acid detergent fibre (ADF) content in different crosses of oilseed rape (Brassica napus)

Genome ◽  
2006 ◽  
Vol 49 (12) ◽  
pp. 1499-1509 ◽  
Author(s):  
Ana Gloria Badani ◽  
Rod J. Snowdon ◽  
Benjamin Wittkop ◽  
Florin D. Lipsa ◽  
Roland Baetzel ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Dominant Gene ◽  
Fibre Content ◽  
Clear Correlation ◽  
Potential Candidate ◽  
Seed Colour ◽  
Yellow Seed ◽  
Acid Detergent Fibre ◽  
Major Qtl

Quantitative trait loci (QTLs) contributing to yellow seed colour and acid detergent fibre (ADF) were localized and compared in 3 mapping populations developed from 2 crosses (designated ‘YE1’ and ‘YE2’) between 2 distinct sources of true-breeding yellow-seeded oilseed rape (Brassica napus) and 2 different black-seeded genotypes. A clear correlation was observed between seed colour and ADF content in both crosses. In all 3 populations, a major QTL, with a large effect on both seed colour and ADF in multiple environments, was detected at the same position on chromosome N18. In YE1, a second minor QTL, with a small effect on seed colour but not on ADF content, was localized on chromosome N1. In YE2, no QTL was observed on N1; however, 2 minor seed-colour loci were localized to N15 and N5. A second major QTL for ADF was localized in YE1 on N13; in YE2, no other QTLs for ADF were detected. Combined QTL and segregation data for seed colour and ADF content in the different populations suggest that a partially dominant B. napus gene for seed colour on N18 contributes to a reduction in fibre content in different yellow-seeded B. napus genotypes. The other QTLs that were identified appear to represent different genes in the 2 yellow-seeded rapeseed sources, which, in each case, affect only fibre content or seed colour, respectively. Potential candidate genes and implications for marker-assisted breeding of oilseed rape with reduced seed dietary fibre content are discussed.

Identification of a major gene and RAPD markers for yellow seed coat colour in Brassica napus

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1077-1082 ◽  
Author(s):  
Daryl J Somers ◽  
Gerhard Rakow ◽  
Vinod K Prabhu ◽  
Ken RD Friesen
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Rapd Markers ◽  
Major Gene ◽  
Coat Colour ◽  
Seed Coat Colour ◽  
Yellow Seed ◽  
Dh Population ◽  
Seed Trait ◽  
Yellow Seed Coat

The development of yellow-seeded Brassica napus for improving the canola-meal quality characteristics of lower fibre content and higher protein content has been restricted because no yellow-seeded forms of B. napus exist, and their conventional development requires interspecific introgression of yellow seed coat colour genes from related species. A doubled-haploid (DH) population derived from the F1 generation of the cross 'Apollo' (black-seeded) × YN90-1016 (yellow-seeded) B. napus was analysed via bulked segregant analysis to identify molecular markers associated with the yellow-seed trait in B. napus for future implementation in marker-assisted breeding. A single major gene (pigment 1) flanked by eight RAPD markers was identified co-segregating with the yellow seed coat colour trait in the population. This gene explained over 72% of the phenotypic variation in seed coat colour. Further analysis of the yellow-seeded portion of this DH population revealed two additional genes favouring 'Apollo' alleles, explaining 11 and 8.5%, respectively, of the yellow seed coat colour variation. The data suggested that there is a dominant, epistatic interaction between the pigment 1 locus and the two additional genes. The potential of the markers to be implemented in plant breeding for the yellow-seed trait in B. napus is discussed.Key words: Brassica napus, yellow seed, RAPD.

scholarly journals Detection and Effects of a Homeologous Reciprocal Transposition in Brassica napus

Genetics ◽  
2003 ◽  
Vol 165 (3) ◽  
pp. 1569-1577
Author(s):  
Thomas C Osborn ◽  
David V Butrulle ◽  
Andrew G Sharpe ◽  
Kathryn J Pickering ◽  
Isobel A P Parkin ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Mapping ◽  
Doubled Haploid ◽  
Selective Advantage ◽  
F1 Hybrids ◽  
Rflp Markers ◽  
Cytological Analysis ◽  
Linkage Groups ◽  
Homeologous Chromosomes ◽  
Seed Yields

Abstract A reciprocal chromosomal transposition was identified in several annual oilseed Brassica napus genotypes used as parents in crosses to biennial genotypes for genetic mapping studies. The transposition involved an exchange of interstitial homeologous regions on linkage groups N7 and N16, and its detection was made possible by the use of segregating populations of doubled haploid lines and codominant RFLP markers. RFLP probes detected pairs of homeologous loci on N7 and N16 for which the annual and biennial parents had identical alleles in regions expected to be homeologous. The existence of an interstitial reciprocal transposition was confirmed by cytological analysis of synaptonemal complexes of annual × biennial F1 hybrids. Although it included approximately one-third of the physical length of the N7 and N16 chromosomes, few recombination events within the region were recovered in the progenies of the hybrids. Significantly higher seed yields were associated with the parental configurations of the rearrangement in segregating progenies. These progenies contained complete complements of homeologous chromosomes from the diploid progenitors of B. napus, and thus their higher seed yields provide evidence for the selective advantage of allopolyploidy through the fixation of intergenomic heterozygosity.

scholarly journals A High-Density Genetic Map Identifies a Novel Major QTL for Boron Efficiency in Oilseed Rape (Brassica napus L.)

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112089 ◽  
Author(s):  
Didi Zhang ◽  
Yingpeng Hua ◽  
Xiaohua Wang ◽  
Hua Zhao ◽  
Lei Shi ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Map ◽  
Oilseed Rape ◽  
High Density ◽  
Brassica Napus L ◽  
Boron Efficiency ◽  
Major Qtl
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