Identification of QTLs involved in pod-shatter resistance in Brassica napus L.

2012 ◽  
Vol 63 (12) ◽  
pp. 1082 ◽  
Author(s):  
Y. C. Wen ◽  
S. F. Zhang ◽  
B. Yi ◽  
J. Wen ◽  
J. P. Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Phenotypic Variation ◽  
Linkage Groups ◽  
Brassica Napus L ◽  
Dh Population ◽  
Seed Loss ◽  
Pod Shatter ◽  
Rapeseed Production ◽  
Simple Sequence ◽  
Selection Of

Seed loss caused by pod-shatter during harvesting is one of the main problems in rapeseed production worldwide. Quantitative trait loci (QTLs) for pod-shatter based on genetic mapping would help breeders develop cultivars resistant to pod-shatter. In this study, we constructed a genetic map of Brassica napus containing 107 simple sequence repeat (SSR) markers and 68 sequence-related amplified polymorphism (SRAP) markers using a doubled-haploid (DH) population of 276 lines derived from the cross H155 × Qva. This map covered 1382.8 cM with an average marker interval of 7.9 cM. In total, 13 QTLs for pod-shatter resistance were identified in this DH population at two experimental sites (in Wuhan and Zhengzhou); three of the QTLs were present at both locations. At Zhengzhou, nine QTLs, identified in linkage groups A1, A7, A8, C5, and C8, together explained 49.0% of the phenotypic variation. At Wuhan, four QTLs were mapped on the A1, A4, A7, and C8 linkage groups. These QTLs explained 38.6% of the phenotypic variation. These results may serve as a valuable basis for further molecular dissection of pod-shatter resistance in B. napus, and for development of the markers related to QTLs that may be useful for marker-assisted selection of pod-shatter resistant cultivars.

scholarly journals MORPHOLOGICAL CHARACTERISTICS OF BEE POLLEN OBTAINED FROM BRASSICA NAPUS L.

2016 ◽  
Vol 18 (2) ◽  
pp. 73-78
Author(s):  
N.M. Redina ◽  
L.O. Adamchuk ◽  
N.V. Nikolaieva ◽  
J. Brindza
Keyword(s):  
Brassica Napus ◽  
Local Population ◽  
Morphological Characteristics ◽  
Morphometric Measurements ◽  
Brassica Napus L ◽  
Pollen Collection ◽  
Bee Pollen ◽  
Total Pollen ◽  
Protein Feed ◽  
Selection Of

The purpose of this work was to research the morphological characteristics of monofloral bee pollen obtained from Brassica napus L. The formulated goals were: determine the period of bee pollen receiving; implement the selection of bee pollen samples during blooming period of species; establish the monoflorality ratio of the total pollen collection; explore the morphometric indicators of bee pollen by the parameters of pollen lump, shaping level and weight. Bee pollen collection was carried out in Skvyra district of Kyiv region from local population of Apis mellifera L. families. Morphometric measurements were performed in the Institute of Biodiversity Conservation and Biosafety, Slovak University of Agriculture in Nitra. Here were determined the amount of bee pollen per one day brought by one family: from  27.04 to 03.05.16 is 50.8 ± 2.25 g; from 04.05 to 06.05.16 – 100.0 ± 3.33 g; from 07.05 to 17.05.16 – 270.7 ± 11.79 g; from 18.05 to 22.05.16 – 100.5 ± 3.028 g; from 23.05 – 25.05.16 – 39.8 ± 2.74 g. Here were defined the morphological parameters of bee pollen lump from B. napus: length is in the range from 3.40 ± 0.061 mm to 3.66 ± 0.048 mm; width is in the range from 2.88 ± 0.059 mm to 3.26 ± 0.067 mm; weight is in the range from 8.65 ± 0.317 mg to 11.31 ± 0.241 mg; shaping level of bee pollen is in the range 4.62 – 4.96 points. The total monoflorality ratio constitutes from 79.94 ± 0.619 % to 98.00 ± 0.202 %. The obtained results confirmed that the activity of bee flying depends on the blooming period and intensity of the nectar secretion, on the collection of protein feed from B. napus. Pollen lumps from Brassica napus L. bee pollen were large, dense, regular shape without splits, which specified the possibility of its use for the producing on a commercial scale.  

Genetic structure of Plasmodiophora brassicae populations virulent on clubroot resistant canola (Brassica napus)

Plant Disease ◽  
2021 ◽  
Author(s):  
Homa Askarian ◽  
Alireza Akhavan ◽  
Leonardo Galindo González ◽  
Sheau-Fang Hwang ◽  
Stephen Ernest Strelkov
Keyword(s):  
Brassica Napus ◽  
Genetic Structure ◽  
Plasmodiophora Brassicae ◽  
Single Spore ◽  
Specific Primers ◽  
Brassica Napus L ◽  
Before And After ◽  
Differential Hosts ◽  
And Migration ◽  
Simple Sequence

Clubroot, caused by Plasmodiophora brassicae Woronin, is a significant threat to the canola (Brassica napus L.) industry in Canada. Clubroot resistance has been overcome in more than 200 fields since 2013, representing one of the biggest challenges to sustainable canola production. The genetic structure of 36 single-spore isolates derived from 12 field isolates of P. brassicae collected before and after the introduction of clubroot resistant (CR) canola cultivars (2005-2014) was evaluated by simple sequence repeat (SSR) marker analysis. Polymorphisms were detected in 32 loci with the identification of 93 distinct alleles. A low level of genetic diversity was found among the single-spore isolates. Haploid linkage disequilibrium and number of migrants suggested that recombination and migration were rare or almost absent in the tested P. brassicae population. A relatively clear relationship was found between the genetic structure and virulence phenotypes of the pathogen as defined on the differential hosts of Somé et al., Williams and the Canadian Clubroot Differential (CCD) set. Although genetic variability within each pathotype group, as classified on each differential system, was low, significant genetic differentiation was observed among the pathotypes. The highest correlation between genetic structure and virulence was found among matrices produced with genetic data and the hosts of the CCD set, with a threshold index of disease of 50% to distinguish susceptible from resistant reactions. Genetically homogeneous single-spore isolates provided a more complete and clearer picture of the population genetic structure of P. brassicae, and the results suggest some promise for the development of pathotype-specific primers.

CRISPR/Cas9-mediated genome editing reveals differences in the contribution of INDEHISCENT homologues to pod shatter resistance in Brassica napus L.

2019 ◽  
Vol 132 (7) ◽  
pp. 2111-2123 ◽  
Author(s):  
Yungu Zhai ◽  
Shengli Cai ◽  
Limin Hu ◽  
Yang Yang ◽  
Olalekan Amoo ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genome Editing ◽  
Brassica Napus L ◽  
Pod Shatter

scholarly journals Marker assisted selection of new high oleic and low linolenic winter oilseed rape (Brassica napus L.) inbred lines revealing good agricultural value

PLoS ONE ◽  
2020 ◽  
Vol 15 (6) ◽  
pp. e0233959 ◽  
Author(s):  
Stanisław Spasibionek ◽  
Katarzyna Mikołajczyk ◽  
Hanna Ćwiek–Kupczyńska ◽  
Teresa Piętka ◽  
Krystyna Krótka ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Marker Assisted Selection ◽  
Inbred Lines ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
High Oleic ◽  
Low Linolenic ◽  
Selection Of

Novel and major QTL for branch angle detected by using DH population from an exotic introgression in rapeseed (Brassica napus L.)

2017 ◽  
Vol 131 (1) ◽  
pp. 67-78 ◽  
Author(s):  
Yusen Shen ◽  
Yi Yang ◽  
Ensheng Xu ◽  
Xianhong Ge ◽  
Yang Xiang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Napus L ◽  
Dh Population ◽  
Branch Angle ◽  
Major Qtl

QTL analysis of yield-related traits and their association with functional markers in Brassica napus L.

2007 ◽  
Vol 58 (8) ◽  
pp. 759 ◽  
Author(s):  
Yuanyuan Li ◽  
Jinxiong Shen ◽  
Tonghua Wang ◽  
Qingfang Chen ◽  
Xingguo Zhang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Agronomic Traits ◽  
High Yield ◽  
Functional Markers ◽  
Multiple Traits ◽  
Brassica Napus L ◽  
Breeding Programs ◽  
F2 Population ◽  
Expressed Sequence ◽  
Simple Sequence

Yield is one of the most important traits in Brassica napus breeding programs. Quantitative trait loci (QTLs) for yield-related traits based on genetic mapping would help breeders to develop high-yield cultivars. In this study, a genetic linkage map of B. napus, containing 142 sequence-related amplified polymorphism (SRAP) markers, 163 functional markers, 160 simple sequence repeat (SSR) markers, and 117 amplified fragment length polymorphism (AFLP) markers, was constructed in an F2 population of 184 individuals resulting from the cross SI-1300 × Eagle. This map covered 2054.51 cM with an average marker interval of 3.53 cM. Subsequently, QTLs were detected for 12 yield-related traits in Wuhan and Jingmen. In total, 133 QTLs were identified, including 14 consistent ones across the 2 locations. Fifteen of 20 linkage groups (LGs) were found to have QTLs for the 12 traits investigated, and most of the QTLs were clustered, especially on LGs N2 and N7, where similar QTL positions were identified for multiple traits. Eight of 10 QTLs for yield per plant (YP) were also associated with number of seeds per silique (SS), number of siliques per plant (SP), and/or 1000-seed weight (SW). In addition, 45 functional markers involved in 39 expressed sequence tags (ESTs) were linked to the QTLs of 12 traits. The present results may serve as a valuable basis for further molecular dissection of agronomic traits in B. napus, and the markers related to QTLs may offer promising possible makers for marker assisted selection.

scholarly journals Early in vitro selection of winter oilseed rape (Brassica napus L.) plants with the fertility restorer gene for CMS Shaan 2A via non-destructive molecular analysis of microspore-derived embryos

2019 ◽  
Vol 55 (No. 4) ◽  
pp. 162-165
Author(s):  
Miroslav Klíma ◽  
Eva Jozová ◽  
Irena Jelínková ◽  
Vratislav Kučera ◽  
Shengwu Hu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Restorer Gene ◽  
Brassica Napus L ◽  
Rf Gene ◽  
Dh Lines ◽  
Non Destructive ◽  
Selection Of

Cotyledons of 201 microspore-derived oilseed rape (Brassica napus) embryos were analysed for the presence of the cytoplasmic male sterility Shaan 2A restorer gene using the primer pair 3F/4R. In total, 172 plants regenerated and were grown to the generative stage. Among phenotypically non-haploid flowering plants, there was a broad spectrum of different flower types from normally developed flowers to flowers with short anthers, stamens, or deformed flowers with atypically developed floral organs, often with traces of pollen. For this reason, only 115 typically fertile or sterile accessions were selected for further phenotype-genotype comparisons. In 14 plants (12.2%) the phenotype did not match the genotype: two plants were marked as fertile, but without the Rf gene. Vice versa, in 12 sterile plants the Rf gene was determined. Nevertheless, the two fertile plants did not produce any pods after self-pollination. In total, 47 doubled haploid (DH) lines with good seed yield, carrying the Rf gene were harvested.  

scholarly journals Study on Growth and Flowering Characteristics in the Spring Sowing for Selection of Rapeseed (Brassica napus L.) Varieties

2015 ◽  
Vol 28 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Kwang-Soo Kim ◽  
Su-Ok Ha ◽  
Yong-Hwa Lee ◽  
Young-Seok Jang ◽  
In-Hu Choi
Keyword(s):  
Brassica Napus ◽  
Brassica Napus L ◽  
Spring Sowing ◽  
Selection Of
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