Colocalization of a partially dominant gene for yellow seed colour with a major QTL influencing acid detergent fibre (ADF) content in different crosses of oilseed rape (Brassica napus)

Genome ◽  
2006 ◽  
Vol 49 (12) ◽  
pp. 1499-1509 ◽  
Author(s):  
Ana Gloria Badani ◽  
Rod J. Snowdon ◽  
Benjamin Wittkop ◽  
Florin D. Lipsa ◽  
Roland Baetzel ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Dominant Gene ◽  
Fibre Content ◽  
Clear Correlation ◽  
Potential Candidate ◽  
Seed Colour ◽  
Yellow Seed ◽  
Acid Detergent Fibre ◽  
Major Qtl

Quantitative trait loci (QTLs) contributing to yellow seed colour and acid detergent fibre (ADF) were localized and compared in 3 mapping populations developed from 2 crosses (designated ‘YE1’ and ‘YE2’) between 2 distinct sources of true-breeding yellow-seeded oilseed rape (Brassica napus) and 2 different black-seeded genotypes. A clear correlation was observed between seed colour and ADF content in both crosses. In all 3 populations, a major QTL, with a large effect on both seed colour and ADF in multiple environments, was detected at the same position on chromosome N18. In YE1, a second minor QTL, with a small effect on seed colour but not on ADF content, was localized on chromosome N1. In YE2, no QTL was observed on N1; however, 2 minor seed-colour loci were localized to N15 and N5. A second major QTL for ADF was localized in YE1 on N13; in YE2, no other QTLs for ADF were detected. Combined QTL and segregation data for seed colour and ADF content in the different populations suggest that a partially dominant B. napus gene for seed colour on N18 contributes to a reduction in fibre content in different yellow-seeded B. napus genotypes. The other QTLs that were identified appear to represent different genes in the 2 yellow-seeded rapeseed sources, which, in each case, affect only fibre content or seed colour, respectively. Potential candidate genes and implications for marker-assisted breeding of oilseed rape with reduced seed dietary fibre content are discussed.

Identification and inheritance of a partially dominant gene for yellow seed colour in Brassica napus

2005 ◽  
Vol 124 (1) ◽  
pp. 9-12 ◽  
Author(s):  
X. P. Liu ◽  
J. X. Tu ◽  
B. Y. Chen ◽  
T. D. Fu
Keyword(s):  
Brassica Napus ◽  
Dominant Gene ◽  
Seed Colour ◽  
Yellow Seed

scholarly journals A High-Density Genetic Map Identifies a Novel Major QTL for Boron Efficiency in Oilseed Rape (Brassica napus L.)

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112089 ◽  
Author(s):  
Didi Zhang ◽  
Yingpeng Hua ◽  
Xiaohua Wang ◽  
Hua Zhao ◽  
Lei Shi ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Map ◽  
Oilseed Rape ◽  
High Density ◽  
Brassica Napus L ◽  
Boron Efficiency ◽  
Major Qtl

Identification of a major QTL for silique length and seed weight in oilseed rape (Brassica napus L.)

2012 ◽  
Vol 125 (2) ◽  
pp. 285-296 ◽  
Author(s):  
Pu Yang ◽  
Chang Shu ◽  
Lin Chen ◽  
Jinsong Xu ◽  
Jiangsheng Wu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Seed Weight ◽  
Brassica Napus L ◽  
Silique Length ◽  
Major Qtl

scholarly journals Identification and QTL mapping of resistance to Turnip yellows virus (TuYV) in oilseed rape, Brassica napus

2019 ◽  
Vol 133 (2) ◽  
pp. 383-393 ◽  
Author(s):  
Dieter Hackenberg ◽  
Elvis Asare-Bediako ◽  
Adam Baker ◽  
Peter Walley ◽  
Carol Jenner ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Qtl Mapping ◽  
Oilseed Rape ◽  
Genetic Resource ◽  
Mapping Population ◽  
Resistant Parent ◽  
Potato Aphid ◽  
Turnip Yellows Virus ◽  
Peach Potato Aphid ◽  
Major Qtl

Abstract Key message Partially dominant resistance to Turnip yellows virus associated with one major QTL was identified in the natural allotetraploid oilseed rape cultivar Yudal. Abstract Turnip yellows virus (TuYV) is transmitted by the peach-potato aphid (Myzus persicae) and causes severe yield losses in commercial oilseed rape crops (Brassica napus). There is currently only one genetic resource for resistance to TuYV available in brassica, which was identified in the re-synthesised B. napus line ‘R54’. In our study, 27 mostly homozygous B. napus accessions, either doubled-haploid (DH) or inbred lines, representing a diverse subset of the B. napus genepool, were screened for TuYV resistance/susceptibility. Partial resistance to TuYV was identified in the Korean spring oilseed rape, B. napus variety Yudal, whilst the dwarf French winter oilseed rape line Darmor-bzh was susceptible. QTL mapping using the established Darmor-bzh × Yudal DH mapping population (DYDH) revealed one major QTL explaining 36% and 18% of the phenotypic variation in two independent experiments. A DYDH line was crossed to Yudal, and reciprocal backcross (BC1) populations from the F1 with either the susceptible or resistant parent revealed the dominant inheritance of the TuYV resistance. The QTL on ChrA04 was verified in the segregating BC1 population. A second minor QTL on ChrC05 was identified in one of the two DYDH experiments, and it was not observed in the BC1 population. The TuYV resistance QTL in ‘R54’ is within the QTL interval on Chr A04 of Yudal; however, the markers co-segregating with the ‘R54’ resistance are not conserved in Yudal, suggesting an independent origin of the TuYV resistances. This is the first report of the QTL mapping of TuYV resistance in natural B. napus.

Dissection of a major QTL for seed colour and fibre content inBrassica napusreveals colocalization with candidate genes for phenylpropanoid biosynthesis and flavonoid deposition

2013 ◽  
Vol 132 (4) ◽  
pp. 382-389 ◽  
Author(s):  
Anna Stein ◽  
Benjamin Wittkop ◽  
Liezhao Liu ◽  
Christian Obermeier ◽  
Wolfgang Friedt ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Fibre Content ◽  
Seed Colour ◽  
Phenylpropanoid Biosynthesis ◽  
Major Qtl

Localization of QTLs for seed color using recombinant inbred lines of Brassica napus in different environments

Genome ◽  
2007 ◽  
Vol 50 (9) ◽  
pp. 840-854 ◽  
Author(s):  
Fu-You Fu ◽  
Lie-Zhao Liu ◽  
You-Rong Chai ◽  
Li Chen ◽  
Tao Yang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Candidate Gene ◽  
Recombinant Inbred ◽  
Seed Color ◽  
Srap Marker ◽  
Linkage Groups ◽  
Yellow Seed ◽  
Seed Trait ◽  
Major Qtl ◽  
Selection Of

Yellow seed is one of the most important traits of Brassica napus L. Efficient selection of the yellow-seed trait is one of the most important objectives in oilseed rape breeding. Two recombinant inbred line (RIL) populations (RIL-1 and RIL-2) were analyzed for 2 years at 2 locations. Four hundred and twenty SSR, RAPD, and SRAP marker loci covering 1744 cM were mapped in 26 linkage groups of RIL-1, while 265 loci covering 1135 cM were mapped in 20 linkage groups of RIL-2. A total of 19 QTLs were detected in the 2 populations. A major QTL was detected adjacent to the same marker (EM11ME20/200) in both maps in both years. This major QTL could explain 53.71%, 39.34%, 42.42%, 30.18%, 24.86%, and 15.08% of phenotypic variation in 6 combinations (location × year × population). BLASTn analysis of the sequences of the markers flanking the major QTL revealed that the homologous region corresponding to this major QTL was anchored between genes At5g44440 and At5g49640 of Arabidopsis thaliana chromosome 5 (At C5). Based on comparative genomic analysis, the bifunctional gene TT10 is nearest to the homologue of EM11ME20/200 on At C5 and can be considered an important candidate gene for the major QTL identified here. Besides providing an effective strategy for marker-assisted selection of the yellow-seed trait in B. napus, our results also provide important clues for cloning of the candidate gene corresponding to this major QTL.

scholarly journals Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mao Yang ◽  
Jianbo He ◽  
Shubei Wan ◽  
Weiyan Li ◽  
Wenjing Chen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Height ◽  
Oilseed Rape ◽  
Molecular Mechanisms ◽  
Dominant Gene ◽  
Planting Density ◽  
Protein Sequence Analysis ◽  
Dwarf Phenotype ◽  
Lodging Resistance ◽  
Dwarfing Gene

Abstract Background Plant height is an important architecture trait which is a fundamental yield-determining trait in crops. Variety with dwarf or semi-dwarf phenotype is a major objective in the breeding because dwarfing architecture can help to increase harvest index, increase planting density, enhance lodging resistance, and thus be suitable for mechanization harvest. Although some germplasm or genes associated with dwarfing plant type have been carried out. The molecular mechanisms underlying dwarfism in oilseed rape (Brassica napus L.) are poorly understood, restricting the progress of breeding dwarf varieties in this species. Here, we report a new dwarf mutant Bndwarf2 from our B. napus germplasm. We studied its inheritance and mapped the dwarf locus BnDWARF2. Results The inheritance analysis showed that the dwarfism phenotype was controlled by one semi-dominant gene, which was mapped in an interval of 787.88 kb on the C04 chromosome of B. napus by Illumina Brassica 60 K Bead Chip Array. To fine-map BnDWARF2, 318 simple sequence repeat (SSR) primers were designed to uniformly cover the mapping interval. Among them, 15 polymorphic primers that narrowed down the BnDWARF2 locus to 34.62 kb were detected using a F2:3 family population with 889 individuals. Protein sequence analysis showed that only BnaC04.BIL1 (BnaC04g41660D) had two amino acid residues substitutions (Thr187Ser and Gln399His) between ZS11 and Bndwarf2, which encoding a GLYCOGEN SYNTHASE KINASE 3 (GSK3-like). The quantitative real-time PCR (qRT-PCR) analysis showed that the BnaC04.BIL1 gene expressed in all tissues of oilseed rape. Subcellular localization experiment showed that BnaC04.BIL1 was localized in the nucleus in tobacco leaf cells. Genetic transformation experiments confirmed that the BnaC04.BIL1 is responsible for the plant dwarf phenotype in the Bndwarf2 mutants. Overexpression of BnaC04.BIL1 reduced plant height, but also resulted in compact plant architecture. Conclusions A dominant dwarfing gene, BnaC04.BIL1, encodes an GSK3-like that negatively regulates plant height, was mapped and isolated. Our identification of a distinct gene locus may help to improve lodging resistance in oilseed rape.

Regional association analysis delineates a sequenced chromosome region influencing antinutritive seed meal compounds in oilseed rapeThis article is one of a selection of papers from the conference “Exploiting Genome-wide Association in Oilseed Brassicas: a model for genetic improvement of major OECD crops for sustainable farming”.

Genome ◽  
2010 ◽  
Vol 53 (11) ◽  
pp. 917-928 ◽  
Author(s):  
R. J. Snowdon ◽  
B. Wittkop ◽  
A. Rezaidad ◽  
M. Hasan ◽  
F. Lipsa ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ssr Markers ◽  
Association Analysis ◽  
Oilseed Rape ◽  
Fibre Content ◽  
Seed Meal ◽  
Seed Colour ◽  
Regional Association ◽  
Genome Wide ◽  
Regional Association Analysis

This study describes the use of regional association analyses to delineate a sequenced region of a Brassica napus chromosome with a significant effect on antinutritive seed meal compounds in oilseed rape. A major quantitative trait locus (QTL) influencing seed colour, fibre content, and phenolic compounds was mapped to the same position on B. napus chromosome A9 in biparental mapping populations from two different yellow-seeded × black-seeded B. napus crosses. Sequences of markers spanning the QTL region identified synteny to a sequence contig from the corresponding chromosome A9 in Brassica rapa . Remapping of sequence-derived markers originating from the B. rapa sequence contig confirmed their position within the QTL. One of these markers also mapped to a seed colour and fibre QTL on the same chromosome in a black-seeded × black-seeded B. napus cross. Consequently, regional association analysis was performed in a genetically diverse panel of dark-seeded, winter-type oilseed rape accessions. For this we used closely spaced simple sequence repeat (SSR) markers spanning the sequence contig covering the QTL region. Correction for population structure was performed using a set of genome-wide SSR markers. The identification of QTL-derived markers with significant associations to seed colour, fibre content, and phenolic compounds in the association panel enabled the identification of positional and functional candidate genes for B. napus seed meal quality within a small segment of the B. rapa genome sequence.

Identification of two major QTL for yellow seed color in two crosses of resynthesized Brassica napus line No. 2127-17

2010 ◽  
Vol 28 (3) ◽  
pp. 335-342 ◽  
Author(s):  
Yan Zhang ◽  
Xia Li ◽  
Wei Chen ◽  
Bin Yi ◽  
Jing Wen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Color ◽  
Yellow Seed ◽  
Resynthesized Brassica Napus ◽  
Napus Line ◽  
Major Qtl
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