Establishment of Walking Catfish (Clarias batrachus) Cell Lines and Development of a Channel Catfish (Ictalurus punctatus) Virus Vaccine

1981 ◽  
Vol 38 (8) ◽  
pp. 925-930 ◽  
Author(s):  
Edward J. Noga ◽  
James X. Hartmann
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Vaccine Strain ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Kidney Cell ◽  
Clarias Batrachus ◽  
Fish Cell ◽  
Wild Type ◽  
Channel Catfish Virus

Cell lines from apparently normal gill, gonad, and kidney tissue of adult walking catfish (Clarias batrachus) were established and have been subcultured 75, 80, and 95 times, respectively. The cells were propagated in a modified Ham's F-12 medium at 25 °C but are capable of growth at 37 °C. The gill and kidney cells are the first to have been established from these tissues in fish. The kidney cell line is fibroblastic, the gonad is a mixed epithelioid–fibroblastic type, and the gill is pleomorphic. All three cell lines are susceptible to channel catfish virus (CCV), exhibiting cell fusion and other marked cytopathic effects. A live attenuated vaccine strain of CCV was produced by repeated passage of a virulent strain of CCV in kidney cell cultures. The vaccine strain is less virulent than the wild-type CCV and protects fingerling catfish against challenge with wild-type virus.Key words: channel catfish virus, Clarias batrachus, fish cell cultures, vaccine

Characteristics of a Previously Unidentified Virus from Channel Catfish (Ictalurus punctatus)

1984 ◽  
Vol 41 (5) ◽  
pp. 807-811 ◽  
Author(s):  
Donald F. Amend ◽  
Terry McDowell ◽  
Ronald P. Hedrick
Keyword(s):  
Cell Lines ◽  
Crassostrea Virginica ◽  
Virus Replication ◽  
Virus Strain ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Average Diameter ◽  
Virus Infectivity ◽  
Channel Catfish Virus ◽  
The Family

A virus (strain F82-34) was isolated from channel catfish (Ictalurus punctatus) that was clearly distinct from channel catfish virus (CCV). The virus induced extensive syncytia in channel catfish ovary (CCO) cells incubated at 26 °C. The BB and CHSE-214 cell lines supported virus replication but the FHM line was refractory. Virus replication was optimal at 25 °C where, after 96 h, approximately 50% of the virus was found to be released from CCO cells. Virus infectivity was stable for 1 mo at4, 16, and 23 °C and for 5 mo at −20 and −8Q °C but was lost after 7 d at45 °C. Partially purified virions are double-shelled hexagonal capsids characteristic of reoviruses and have an average diameter of 75 nm. F82-34 was related to but distinct from two other reoviruses from fish in neutralization studies; these three viruses, and possibly a fourth from American oysters (Crassostrea virginica), appear to represent a new taxonomic group within the family Reoviridae. We therefore propose that this new virus be tentatively designated catfish reovirus (CRV).

Growth Rates of a New Cell Line from Channel Catfish Ovary and Channel Catfish Virus Replication at Different Temperatures

1980 ◽  
Vol 37 (5) ◽  
pp. 871-873 ◽  
Author(s):  
P. R. Bowser ◽  
J. A. Plumb
Keyword(s):  
Cell Line ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Growth Rates ◽  
Frozen Storage ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Fish Cell ◽  
Channel Catfish Virus ◽  
New Cell Line

Growth rates of a new cell line from the channel catfish, Ictalurus punctatus, were determined at temperatures from 10 to 35 °C. Growth was more rapid at 35 °C with the cells having a population doubling time of 12 h, and cell numbers declined at 10 °C. The cells survived frozen storage at −70 °C for up to 6 mo. Replication of channel catfish virus occurred from 10 to 35 °C. Although it was most rapid at 35 °C, maximum replication occurred at 30 °C.Key words: fish viruses, channel catfish virus, herpesvirus, fish cell cultures, fish diseases, cell growth rates

Isolation of channel catfish virus from channel catfish, Ictalurus punctatus (Rafinesque), broodstock*

1985 ◽  
Vol 8 (6) ◽  
pp. 557-561 ◽  
Author(s):  
P. R. BOWSER ◽  
A. D. MUNSON ◽  
H. H. JARBOE ◽  
R. FRANCIS-FLOYD ◽  
P. R. WATERSTRAT
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Channel Catfish Virus

First detection of channel catfish virus associated with mortality of cultured catfish (Ictalurus punctatus, Rafinesque) in Mexico

2007 ◽  
Vol 38 (13) ◽  
pp. 1428-1431 ◽  
Author(s):  
Jesús Genaro Sánchez-Martínez ◽  
Gabriel Aguirre-Guzmán ◽  
Ned Ivan de la Cruz-Hernández ◽  
Julio Martínez-Burnes ◽  
Roberto Pérez-Castañeda ◽  
...  
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Channel Catfish Virus

Development of leukocyte cell lines from the channel catfish (Ictalurus punctatus)

1994 ◽  
Vol 16 (2) ◽  
pp. 117-123 ◽  
Author(s):  
Norman W. Miller ◽  
V. Gregory Chinchar ◽  
L. William Clem
Keyword(s):  
Cell Lines ◽  
Ictalurus Punctatus ◽  
Channel Catfish
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