Characteristics of a Previously Unidentified Virus from Channel Catfish (Ictalurus punctatus)

1984 ◽  
Vol 41 (5) ◽  
pp. 807-811 ◽  
Author(s):  
Donald F. Amend ◽  
Terry McDowell ◽  
Ronald P. Hedrick
Keyword(s):  
Cell Lines ◽  
Crassostrea Virginica ◽  
Virus Replication ◽  
Virus Strain ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Average Diameter ◽  
Virus Infectivity ◽  
Channel Catfish Virus ◽  
The Family

A virus (strain F82-34) was isolated from channel catfish (Ictalurus punctatus) that was clearly distinct from channel catfish virus (CCV). The virus induced extensive syncytia in channel catfish ovary (CCO) cells incubated at 26 °C. The BB and CHSE-214 cell lines supported virus replication but the FHM line was refractory. Virus replication was optimal at 25 °C where, after 96 h, approximately 50% of the virus was found to be released from CCO cells. Virus infectivity was stable for 1 mo at4, 16, and 23 °C and for 5 mo at −20 and −8Q °C but was lost after 7 d at45 °C. Partially purified virions are double-shelled hexagonal capsids characteristic of reoviruses and have an average diameter of 75 nm. F82-34 was related to but distinct from two other reoviruses from fish in neutralization studies; these three viruses, and possibly a fourth from American oysters (Crassostrea virginica), appear to represent a new taxonomic group within the family Reoviridae. We therefore propose that this new virus be tentatively designated catfish reovirus (CRV).

Establishment of Walking Catfish (Clarias batrachus) Cell Lines and Development of a Channel Catfish (Ictalurus punctatus) Virus Vaccine

1981 ◽  
Vol 38 (8) ◽  
pp. 925-930 ◽  
Author(s):  
Edward J. Noga ◽  
James X. Hartmann
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Vaccine Strain ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Kidney Cell ◽  
Clarias Batrachus ◽  
Fish Cell ◽  
Wild Type ◽  
Channel Catfish Virus

Cell lines from apparently normal gill, gonad, and kidney tissue of adult walking catfish (Clarias batrachus) were established and have been subcultured 75, 80, and 95 times, respectively. The cells were propagated in a modified Ham's F-12 medium at 25 °C but are capable of growth at 37 °C. The gill and kidney cells are the first to have been established from these tissues in fish. The kidney cell line is fibroblastic, the gonad is a mixed epithelioid–fibroblastic type, and the gill is pleomorphic. All three cell lines are susceptible to channel catfish virus (CCV), exhibiting cell fusion and other marked cytopathic effects. A live attenuated vaccine strain of CCV was produced by repeated passage of a virulent strain of CCV in kidney cell cultures. The vaccine strain is less virulent than the wild-type CCV and protects fingerling catfish against challenge with wild-type virus.Key words: channel catfish virus, Clarias batrachus, fish cell cultures, vaccine

Isolation of channel catfish virus from channel catfish, Ictalurus punctatus (Rafinesque), broodstock*

1985 ◽  
Vol 8 (6) ◽  
pp. 557-561 ◽  
Author(s):  
P. R. BOWSER ◽  
A. D. MUNSON ◽  
H. H. JARBOE ◽  
R. FRANCIS-FLOYD ◽  
P. R. WATERSTRAT
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Channel Catfish Virus

scholarly journals Theiler's Virus-Induced Intrinsic Apoptosis in M1-D Macrophages Is Bax Mediated and Restricts Virus Infectivity: a Mechanism for Persistence of a Cytolytic Virus

2008 ◽  
Vol 82 (9) ◽  
pp. 4502-4510 ◽  
Author(s):  
Kyung-No Son ◽  
Robert P. Becker ◽  
Patricia Kallio ◽  
Howard L. Lipton
Keyword(s):  
Virus Infection ◽  
Virus Replication ◽  
Virus Infectivity ◽  
Apoptotic Pathway ◽  
Virus Growth ◽  
The Family ◽  
Encephalomyelitis Virus ◽  
D Cells ◽  
Kinetics Of ◽  
Mouse Central Nervous System

ABSTRACT Theiler's murine encephalomyelitis virus (TMEV), a member of the Cardiovirus genus in the family Picornaviridae, is a highly cytolytic virus that produces necrotic death in rodent cells except for macrophages, which undergo apoptosis. In the present study we have analyzed the kinetics of BeAn virus infection in M1-D cells, in order to temporally relate virus replication to the apoptotic signaling events. Apoptosis was associated with early exponential virus growth from 1 to 12 h postinfection (p.i.); however, ≥80% of peak infectivity was lost by 16 to 24 h p.i. The pan-caspase inhibitor qVD-OPh led to significantly higher virus yields, while zVAD-fmk completely inhibited virus replication until 10 h p.i., precluding its assessment in apoptosis. In contrast, while zVAD-fmk significantly inhibited BeAn virus replication in BHK-21 cells at 12 and 16 h p.i., virus replication at these time points was not altered by qVD-OPh. Bax translocation into mitochondria, efflux of cytochrome c into the cytoplasm, and activation of caspases 9 and 3 between ∼8 and 12 h p.i. (all hallmarks of the intrinsic apoptotic pathway) were transiently inhibited by expression of Bcl-2, which is not expressed in M1-D cells. Thus, BeAn virus infection in M1-D macrophages, which restricts virus replication, provides a potential mechanism for modulating TMEV neurovirulence during persistence in the mouse central nervous system.

First detection of channel catfish virus associated with mortality of cultured catfish (Ictalurus punctatus, Rafinesque) in Mexico

2007 ◽  
Vol 38 (13) ◽  
pp. 1428-1431 ◽  
Author(s):  
Jesús Genaro Sánchez-Martínez ◽  
Gabriel Aguirre-Guzmán ◽  
Ned Ivan de la Cruz-Hernández ◽  
Julio Martínez-Burnes ◽  
Roberto Pérez-Castañeda ◽  
...  
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Channel Catfish Virus

Development of leukocyte cell lines from the channel catfish (Ictalurus punctatus)

1994 ◽  
Vol 16 (2) ◽  
pp. 117-123 ◽  
Author(s):  
Norman W. Miller ◽  
V. Gregory Chinchar ◽  
L. William Clem
Keyword(s):  
Cell Lines ◽  
Ictalurus Punctatus ◽  
Channel Catfish
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