Growth Rates of a New Cell Line from Channel Catfish Ovary and Channel Catfish Virus Replication at Different Temperatures

1980 ◽  
Vol 37 (5) ◽  
pp. 871-873 ◽  
Author(s):  
P. R. Bowser ◽  
J. A. Plumb
Keyword(s):  
Cell Line ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Growth Rates ◽  
Frozen Storage ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Fish Cell ◽  
Channel Catfish Virus ◽  
New Cell Line

Growth rates of a new cell line from the channel catfish, Ictalurus punctatus, were determined at temperatures from 10 to 35 °C. Growth was more rapid at 35 °C with the cells having a population doubling time of 12 h, and cell numbers declined at 10 °C. The cells survived frozen storage at −70 °C for up to 6 mo. Replication of channel catfish virus occurred from 10 to 35 °C. Although it was most rapid at 35 °C, maximum replication occurred at 30 °C.Key words: fish viruses, channel catfish virus, herpesvirus, fish cell cultures, fish diseases, cell growth rates

Establishment of Walking Catfish (Clarias batrachus) Cell Lines and Development of a Channel Catfish (Ictalurus punctatus) Virus Vaccine

1981 ◽  
Vol 38 (8) ◽  
pp. 925-930 ◽  
Author(s):  
Edward J. Noga ◽  
James X. Hartmann
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Vaccine Strain ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Kidney Cell ◽  
Clarias Batrachus ◽  
Fish Cell ◽  
Wild Type ◽  
Channel Catfish Virus

Cell lines from apparently normal gill, gonad, and kidney tissue of adult walking catfish (Clarias batrachus) were established and have been subcultured 75, 80, and 95 times, respectively. The cells were propagated in a modified Ham's F-12 medium at 25 °C but are capable of growth at 37 °C. The gill and kidney cells are the first to have been established from these tissues in fish. The kidney cell line is fibroblastic, the gonad is a mixed epithelioid–fibroblastic type, and the gill is pleomorphic. All three cell lines are susceptible to channel catfish virus (CCV), exhibiting cell fusion and other marked cytopathic effects. A live attenuated vaccine strain of CCV was produced by repeated passage of a virulent strain of CCV in kidney cell cultures. The vaccine strain is less virulent than the wild-type CCV and protects fingerling catfish against challenge with wild-type virus.Key words: channel catfish virus, Clarias batrachus, fish cell cultures, vaccine

Characterization of a newly established uterine carcinosarcoma cell line featuring the sarcomatous phenotype of the tumor in vitro

2008 ◽  
Vol 18 (2) ◽  
pp. 339-344 ◽  
Author(s):  
H.-J. Schulten ◽  
J. Wolf-Salgó ◽  
C. Gründker ◽  
B. Gunawan ◽  
L. FÜZESI
Keyword(s):  
Cell Line ◽  
Hormone Receptors ◽  
Tumor Biology ◽  
Growth Factor Receptor ◽  
Population Doubling ◽  
Established Cell Line ◽  
Population Doubling Time ◽  
Uterine Carcinosarcoma ◽  
Specific Staining

We describe the newly established cell line CS-99 derived from a uterine carcinosarcoma retaining features of the sarcomatous phenotype in vitro. CS-99 cells exhibit a mesenchymal morphology with predominantly spindle-shaped cells at nonconfluence turning to pleomorphic appearance at confluence. The mesenchymal phenotype was evidenced immunohistochemically by strong vimentin and moderate SM-actin, which was similar to the sarcomatous component of the primary tumor. P53 was overexpressed in a subset of CS-99 cells. Epithelial membrane antigen was moderately expressed whereas other markers including pan CK, CK 5/6, CK 34, epidermal growth factor receptor, desmin, carcinoembryonic antigen, S100, KIT, ERBB2, and the hormone receptors, estrogen receptor and progesterone receptor revealed either weak or no specific staining in CS-99 cells. High self-renewal capacity corresponded to the population doubling time of 23 h in high passage. CS-99 cells were able to develop three-dimensional tumor spheroids in vitro. Cytogenetic analysis and multicolor fluorescence in situ hybridization of CS-99 demonstrated an almost stable karyotype including numerical changes +8, +18, and +20 and translocations, amongst others der(1)t(1;2), der(1)t(1;7), der(2)t(2;19), der(5)t(5;8), and der(5)t(5;14). Taken together, the cell line CS-99 exhibits strong growths dynamics and a complex but stable karyotype in higher passages, and can be further a useful in vitro model system for studying tumor biology of carcinosarcomas.

scholarly journals Developing an Ephestia kuehniella Hemocyte Cell Line to Assess the Bio-Insecticidal Potential of Microencapsulated Helicoverpa armigera Nucleopolyhedrovirus Against Cotton Bollworm (Lepidoptera: Noctuidae) Larva

2020 ◽  
Vol 113 (5) ◽  
pp. 2086-2095
Author(s):  
Bita Valizadeh ◽  
Samira Samarfard ◽  
Jalal Jalali Sendi ◽  
Thomas P Karbanowicz
Keyword(s):  
Cell Line ◽  
Helicoverpa Armigera ◽  
Ephestia Kuehniella ◽  
Cotton Bollworm ◽  
Population Doubling ◽  
Logarithmic Phase ◽  
Population Doubling Time ◽  
Cottonseed Kernel ◽  
Helicoverpa Armígera ◽  
Lepidoptera Noctuidae

Abstract Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) (genus: Alphabaculovirus, incertae sedis: Baculoviridae) has been used to control Helicoverpa armigera (Hübner). A reproducible and susceptible cell line was prepared from the hemocytes of Ephestia kuehniella in Grace and Ex-Cell 420 media. The population doubling time of these cloned cell cultures during the logarithmic phase were about 2.3 and 3.7 d for Ex-Cell 420 and Grace’s media, respectively. When 60% confluence occurred, cells were infected by viral inoculums. All biochemical compounds were significantly changed relevant to cellular metabolism due to HearNPV infection. In order to improve its stability, two polymer formulations were used, i.e., formulation A (sodium alginate, gelatin, starch, and molasses) and formulation B (cottonseed kernel extract, Bran, glycerol, boric acid, egg white, and sugar). Formulant A provided high photostability by exhibiting 83.2 ± 3% efficacy and 88.66 ± 2.1% original activities remaining after 72 h UV exposure. Percentage original activity remaining of unformulated HearNPV and formulated mixture of B was 38.66 ± 2.6% and 9.33 ± 1.3%, respectively, after 72 h UV-irradiation. The virulence of the HearNPV proliferated from the Ex-Cell medium was similar to the virulence of wild-type HearNPV with LC50 of 7.7×105 OBs/ml. Formulant A, revealed only 20.0 ± 1% reduction in efficacy while the unformulated virus and formulant B faced a reduction of 90.0 ± 3% and 64.0 ± 2% after 72 h of UVA irradiation. Formulant A thus showed a high potential to protect HearNPVs microparticles against UV-inactivation suggesting a new platform for more efficient biological-management of cotton bollworm (specific name Helicoverpa armigera, genus: Helicoverpa, Lepidoptera: Noctuidae) in vivo.

Isolation of channel catfish virus from channel catfish, Ictalurus punctatus (Rafinesque), broodstock*

1985 ◽  
Vol 8 (6) ◽  
pp. 557-561 ◽  
Author(s):  
P. R. BOWSER ◽  
A. D. MUNSON ◽  
H. H. JARBOE ◽  
R. FRANCIS-FLOYD ◽  
P. R. WATERSTRAT
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Channel Catfish Virus

Characteristics of a Previously Unidentified Virus from Channel Catfish (Ictalurus punctatus)

1984 ◽  
Vol 41 (5) ◽  
pp. 807-811 ◽  
Author(s):  
Donald F. Amend ◽  
Terry McDowell ◽  
Ronald P. Hedrick
Keyword(s):  
Cell Lines ◽  
Crassostrea Virginica ◽  
Virus Replication ◽  
Virus Strain ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Average Diameter ◽  
Virus Infectivity ◽  
Channel Catfish Virus ◽  
The Family

A virus (strain F82-34) was isolated from channel catfish (Ictalurus punctatus) that was clearly distinct from channel catfish virus (CCV). The virus induced extensive syncytia in channel catfish ovary (CCO) cells incubated at 26 °C. The BB and CHSE-214 cell lines supported virus replication but the FHM line was refractory. Virus replication was optimal at 25 °C where, after 96 h, approximately 50% of the virus was found to be released from CCO cells. Virus infectivity was stable for 1 mo at4, 16, and 23 °C and for 5 mo at −20 and −8Q °C but was lost after 7 d at45 °C. Partially purified virions are double-shelled hexagonal capsids characteristic of reoviruses and have an average diameter of 75 nm. F82-34 was related to but distinct from two other reoviruses from fish in neutralization studies; these three viruses, and possibly a fourth from American oysters (Crassostrea virginica), appear to represent a new taxonomic group within the family Reoviridae. We therefore propose that this new virus be tentatively designated catfish reovirus (CRV).

Fish cell culture: A new cell line fromCynoscion nebulosus

In Vitro ◽  
1979 ◽  
Vol 15 (2) ◽  
pp. 109-111 ◽  
Author(s):  
B. L. Middlebrooks ◽  
R. D. Ellender ◽  
J. H. Wharton
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Fish Cell ◽  
New Cell Line ◽  
Fish Cell Culture

First detection of channel catfish virus associated with mortality of cultured catfish (Ictalurus punctatus, Rafinesque) in Mexico

2007 ◽  
Vol 38 (13) ◽  
pp. 1428-1431 ◽  
Author(s):  
Jesús Genaro Sánchez-Martínez ◽  
Gabriel Aguirre-Guzmán ◽  
Ned Ivan de la Cruz-Hernández ◽  
Julio Martínez-Burnes ◽  
Roberto Pérez-Castañeda ◽  
...  
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Channel Catfish Virus
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