Blood Oxygen Dissociation Characteristics of the Winter Flounder, Pseudopleuronectes americanus

1975 ◽  
Vol 32 (9) ◽  
pp. 1539-1544 ◽  
Author(s):  
Jonathan B. Hayden ◽  
Joseph J. Cech Jr. ◽  
David W. Bridges
Keyword(s):  
Dissolved Oxygen ◽  
Temperature Effect ◽  
Winter Flounder ◽  
Blood Oxygen ◽  
Pseudopleuronectes Americanus ◽  
Small Temperature ◽  
Oxygen Dissociation ◽  
Root Effects ◽  
Dissociation Curves ◽  
Oxygen Concentrations

Oxygen dissociation curves were determined for winter flounder (Pseudopleuronectes americanus) blood at 5, 10, and 15 C, and [Formula: see text], 8, and 24 mm Hg. Half-saturation tensions (P50’s) and blood oxygen capacities were measured and showed significant Bohr and Root effects. Calculations of Hill’s constant, n, implied no heme-heme interaction in the flounder hemoglobin. The apparent heat of oxygenation, ΔH, was calculated to be smaller than that in most teleostean hemoglobins. This small temperature effect, together with the modest slope of the hyperbolic oxygen dissociation curves, indicate a capacity to live in waters exhibiting a fairly broad range of temperatures and dissolved oxygen concentrations.

scholarly journals Some Aspects of the Respiration of Six Species of Fish From Uganda

1956 ◽  
Vol 33 (1) ◽  
pp. 186-195
Author(s):  
G. R. FISH
Keyword(s):  
Carbon Dioxide ◽  
Dissolved Oxygen ◽  
Oxygen Dissociation ◽  
Dissociation Curves

The oxygen dissociation curves of the haemoglobin of six species of fish from Uganda, and the effect of 25 mm. Hg of carbon dioxide on them are presented graphically. The fish were selected from habitats varying widely in contents of dissolved oxygen and carbon dioxide. The ecology of these species is correlated with differences in the shape and slope of the curve. Records of fish mortalities in Lakes Albert and Victoria are examined in the light of the new data available and suggestions as to the causes are put forward.

Human whole-blood oxygen affinity: effect of carbon monoxide

1984 ◽  
Vol 57 (1) ◽  
pp. 14-20 ◽  
Author(s):  
A. Zwart ◽  
G. Kwant ◽  
B. Oeseburg ◽  
W. G. Zijlstra
Keyword(s):  
Carbon Monoxide ◽  
Whole Blood ◽  
Saturation Pressure ◽  
Oxygen Affinity ◽  
Gradual Change ◽  
Blood Oxygen ◽  
Human Whole Blood ◽  
Oxygen Dissociation ◽  
Blood Oxygen Affinity ◽  
Dissociation Curves

Oxygen dissociation curves (ODC) were recorded in the presence of carboxyhemoglobin fractions (FHbCO) up to 60%. The gradual shift to the left of the ODC at increasing amounts of HbCO was reflected in a gradual fall in the half-saturation pressure of the remaining Hb and was accompanied by a gradual change in the shape of the ODC to a hyperbolic one. The H+ factor (delta log PO2/delta pH) was determined over the entire oxygen saturation (SO2) range at three different FHbCO levels (14, 30, and 52%). At FHbCO = 14 and 30% and for the SO2 range 20–90%, the H+ factor vs. SO2 curve was not significantly different from that in the absence of HbCO. At FHbCO = 52%, however, the value found for the H+ factor (-0.55) was appreciably more negative than in the case of blood containing less than 1% HbCO (-0.44), and there was no dependence on SO2. Comparison of measured and calculated ODCs at varying HbCO fractions showed, for FHbCO less than or equal to 50%, that measured and calculated ODCs coincide over the greater part of the SO2 range. For FHbCO greater than 50%, the measured ODC was situated to the left of the calculated one over the entire SO2 range. We conclude that the heme-heme interaction for CO is appreciably larger than for O2 only for FHbCO greater than 50%, whereas for FHbCO less than 50% there is virtually no difference.

scholarly journals Temperature-Induced Changes in Blood Gas Equilibria in the Albacore, Thunnus Alalunga, a Warm-Bodied Tuna

1984 ◽  
Vol 109 (1) ◽  
pp. 21-34 ◽  
Author(s):  
JOSEPH J. CECH ◽  
R. MICHAEL LAURS ◽  
JEFFREY B. GRAHAM
Keyword(s):  
Temperature Effect ◽  
Core Body Temperature ◽  
Bohr Effect ◽  
Arterial Oxygen ◽  
Blood Gas ◽  
Root Effect ◽  
Arterial Blood ◽  
Oxygen Dissociation ◽  
Thunnus Alalunga ◽  
Dissociation Curves

Samples of unbuffered, whole blood from freshly-caught albacore (Thunnus alalunga Bonnaterre) were equilibrated at 5, 10, 15, 20, 25, 30 and 35 °C and at 0 and 1 % CO2 for construction of oxygen dissociation curves. A strong Bohr effect (−1.17), a negligible Root effect, and a reverse temperature effect (ΔH = +1.72 for 0% CO2 and +0.26 for 1% CO2) characterized these hyperbolic (Hill's n = 1.1) curves. The unusual reverse temperature effect was especially pronounced when blood was quickly warmed or cooled, simulating passage through the heat exchanging, countercurrent vascular rete system of this warm-bodied fish. A diagrammatic model of blood gas dynamics in the rete incorporating these in vitro data illustrates protection of arterial oxygen from premature haemoglobin dissociation and consequent loss to the venous circulation as blood warms in the rete. More conventional temperature effects on the carbon dioxide equilibria of albacore blood lower the Pcoco2 of venous blood being cooled in the rete. This reduces the venous-arterial Pcoco2 gradient, thereby minimizing the diffusion of CO2 to arterial blood with resulting haemoglobin-oxygen dissociation via the strong Bohr effect. The temperature range (10–30 °C) over which the albacore haemoglobin-oxygen binding exhibits the reversed thermal effect closely matches the maximum thermal gradient (ambient water-core body temperature) typically present in this fish, suggesting that its highly specialized haemoglobin-oxygen dissociation characteristics evolved within-and now establishes thermal limits upon-the existing geographic distribution of this species.

Factors Affecting Respiration Rates of Winter Flounder (Pseudopleuronectes americanus)

1971 ◽  
Vol 28 (12) ◽  
pp. 1907-1911 ◽  
Author(s):  
R. A. Voyer ◽  
G. E. Morrison
Keyword(s):  
Body Weight ◽  
Oxygen Uptake ◽  
Dissolved Oxygen ◽  
Winter Flounder ◽  
Pseudopleuronectes Americanus ◽  
Factors Affecting ◽  
Respiration Rates ◽  
Consumption Rates ◽  
Series Of Experiments ◽  
Temperature Interactions

In one series of experiments, average rates of oxygen consumed by winter flounder at 10 C were 35 and 55 mg O2/kg of body weight per hour at 3.5 and 8.6 mg dissolved oxygen (DO)/liter, respectively. At 20 C the average rates of oxygen uptake were 70 at 3.2 mg DO/liter and 97 at 6.3 mg DO/liter. Oxygen consumption rates were significantly greater (P <.05) at 20 C than at 10 C. In two of the three experiments, rates of oxygen uptake were significantly lower (P <.05) among groups of winter flounder maintained at the reduced dissolved oxygen concentrations for 15–23 hr. No dissolved oxygen–temperature interactions were apparent.Results of a second series of experiments indicated respiration rates of winter flounder increased linearly with weight along regression lines having slopes of 0.792 (20 C — 6.9 mg DO/liter), 0.785 (20 C — 4.3 mg DO/liter), 0.720 (10 C — 8.7 mg DO/liter), and 0.746 (10 C — 4.3 mg DO/liter).

Blood Gas Relationships in the Rainbow Trout Salmo Gairdneri

1971 ◽  
Vol 55 (3) ◽  
pp. 695-711
Author(s):  
F. B. EDDY
Keyword(s):  
Carbon Dioxide ◽  
Rainbow Trout ◽  
Ph Value ◽  
Carbon Dioxide Tension ◽  
Salmo Gairdneri ◽  
Hydrogen Ions ◽  
Oxygen Dissociation ◽  
Root Effects ◽  
Dissociation Curves ◽  
Increasing Temperature

1. Oxygen dissociation curves were determined using blood from rainbow trout, acclimated for at least 3 weeks to temperatures of 6, 15 and 20°C. Carbon dioxide tensions in the range 0·3-7 mmHg produced both the Bohr and Root effects in the blood. 2. Increasing temperature, Pco2 and hydrogen ions, whether raised individually or together, caused a decrease in blood O2 affinity (increased value for P50). 3. Blood at low temperatures had a higher pH than blood at high temperatures. This is related to the fact that the ionization constant of water is diminished with decreasing temperature. When blood was 50% saturated and Pco2 was 1 mmHg, the pH value was 8·25 at 6°C, 7·83 at 15°C and 7·62 at 20°C. 4. The factors influencing unloading of oxygen from the blood are discussed. To release the same amount of oxygen from blood, a greater change in carbon dioxide tension is required at 6°C than at higher temperatures. 5. The Bohr effect expressed quantitatively (Δ log P50/Δ pH) was -0·54 at 6°C, -0·57 at 15°C and -0·59 at 20°C. These values are similar to those for the blood of many mammals, and are within the range reported for fish, where whole blood has been used.

Sign in / Sign up

Export Citation Format

Share Document