Rancidity in Lean Fish Muscle.: I. A Proposed Accelerated Copper-Catalyzed Method for Evaluating the Tendency of Fish Muscle to Become Rancid

1964 ◽  
Vol 21 (6) ◽  
pp. 1345-1359 ◽  
Author(s):  
Jill MacLean ◽  
C. H. Castell
Keyword(s):  
Copper Ion ◽  
Thiobarbituric Acid ◽  
Fish Muscle ◽  
Lean Fish ◽  
Acid Reaction

A method that can be carried out within 24–72 hr is suggested for determining the tendency of fish muscle to become rancid. It consists of adding measured, trace amounts of copper ion to muscle that has been blended with water (1:3) followed by storage at 0 °C. Rancidity is observed subjectively by noting the odours that develop and objectively by means of the thiobarbituric acid reaction.

A Simple Method for Estimation of Lipoxygenase and Cyclo-Oxygenase Pathways in Human Platelets - the Use of Thiobarbituric Acid Reaction

1980 ◽  
Vol 44 (02) ◽  
pp. 111-114 ◽  
Author(s):  
Hiroshi Takayama ◽  
Minoru Okuma ◽  
Haruto Uchino
Keyword(s):  
Time Course ◽  
Normal Values ◽  
Human Platelet ◽  
Thiobarbituric Acid ◽  
Human Platelets ◽  
Optimal Conditions ◽  
Experimental Conditions ◽  
Simple Method ◽  
Acid Reaction ◽  
Optimal Ph

SummaryTo develop a simple method for estimation of platelet lipoxygenase (PLO) and cyclo-oxygenase (PCO) pathways, the arachidonic acid (AA) metabolism of human platelet was investigated under various experimental conditions by the use of the thiobarbituric acid (TBA) reaction and a radioisotope technique. A TBA-reactive substance different from malondialdehyde (MDA) via PCO pathway was detected and shown to be derived from the PLO pathway. Since the optimal pH and time course of its formation were different from those of MDA formation via PCO pathway, PLO and PCO pathways were estimated by quantitating the TBA-reactive substances produced by the incubation of AA either with aspirin-treated platelets or with untreated ones, respectively, each under optimal conditions. Normal values expressed in terms of nmol MDA/108 platelets were 1.17±0.34 (M±SD, n = 31) and 0.79±0.15 (n = 31) for PLO and PCO pathways, respectively.

Estimation of 3-Deoxy Sugars by Means of the Malonaldehyde–Thiobarbituric Acid Reaction

Nature ◽  
1960 ◽  
Vol 186 (4719) ◽  
pp. 155-156 ◽  
Author(s):  
MORRIS A. CYNKIN ◽  
GILBERT ASHWELL
Keyword(s):  
Thiobarbituric Acid ◽  
Acid Reaction ◽  
Deoxy Sugars

scholarly journals Determination of Chlorobiphenyls in Seal Blubber, Marine Sediment, and Fish: Interlaboratory Study

1996 ◽  
Vol 79 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Jacob De Boer ◽  
Jaap Van Der Meer ◽  
Udo A Th Brinkman
Keyword(s):  
Muscle Tissue ◽  
Marine Sediment ◽  
Standard Error ◽  
Principal Component ◽  
Fish Muscle ◽  
Interlaboratory Study ◽  
Lean Fish ◽  
Seal Blubber Oil

Abstract Between 1988 and 1994, the International Councilfor the Exploration of the Sea, the Intergovernmental Oceanographic Commission, and the Oslo and Paris Commissions organized a stepwise interlaboratory study for determination of chlorobiphenyls (CBs) inmarine media. The final parts of this study, in which 53 laboratories from 14 countries participated, focused on long-term precision, cleanup, and extraction. Calibration was controlled continuously by analysis of 10 CBs in an unknownsolution. Participants were requested toanalyze 3 CBs in a certified reference material fish oil (6 times); 10 CBs in cleaned and uncleaned marine sediment and seal blubber extracts; and 10 CBs in seal blubber oil, dried marine sediment, and wet, lean fish muscle tissue. The long-term precision study showed that, compared with earlier exercises in which only duplicate analyses were required, repeatability increased about 1.5-fold compared with reproducibility. The mean standard error for reproducibility of determination of 10 CBs in standard solutions improved from 1.22 to 1.15. The standard error improved from 1.36 to 1.28(without CBs 28 and 31) for seal blubberoil and from 1.36 to 1.22 for dried marine sediment. In seal blubber oil and dried marine sediment, the major CBs 118,138,153, and 180 can now bedetermined by thegroup of participating laboratories with a reproducibility of 1.5 (about 50%). No significant differences were found between results for cleaned-up and un cleaned extracts. No acceptable results could be obtained for determination ofCBs in lean fish muscle tissue. Biplots of principal component analyses are extremely helpful in evaluating the data generated by this type of study.

Synthesis and biological activity of new 1,3,4-thiadiazole derivatives

2006 ◽  
Vol 60 (1) ◽  
Author(s):  
A. Aly ◽  
R. El-Sayed
Keyword(s):  
Biological Activity ◽  
Carbon Disulfide ◽  
Acetyl Chloride ◽  
Thioglycolic Acid ◽  
Thiobarbituric Acid ◽  
Aromatic Aldehydes ◽  
Phenyl Isocyanate ◽  
Phenyl Isothiocyanate ◽  
Acid Reaction ◽  
Thiadiazole Derivatives

AbstractThe aminothiadiazole (II) on treatment with aromatic aldehydes yielded Schiff bases, which cyclized to thiazolidinone derivatives by reaction with thioglycolic acid. Reaction of II with phenyl isocyanate and phenyl isothiocyanate afforded the carbamide and carbothiamide derivatives, respectively, which on reaction with malonic acid in acetyl chloride gave barbituric and thiobarbituric acid derivatives. However, reaction of carbon disulfide and methyl iodide with II gave dithiocarbamidate derivative which on treatment with ethylenediamine or o-phenylenediamine gave the condensed N-imidazolylthiadiazolylamine derivatives.

Determination of malonaldehyde in human plasma: elimination of spectral interferences in the 2-thiobarbituric acid reaction

The Analyst ◽  
1993 ◽  
Vol 118 (1) ◽  
pp. 89 ◽  
Author(s):  
Anunciaci�n Espinosa-Mansilla ◽  
Francisco Salinas ◽  
Amparo Rubio Leal
Keyword(s):  
Human Plasma ◽  
Thiobarbituric Acid ◽  
Spectral Interferences ◽  
Acid Reaction
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