Estimation of 3-Deoxy Sugars by Means of the Malonaldehyde–Thiobarbituric Acid Reaction

Nature ◽  
1960 ◽  
Vol 186 (4719) ◽  
pp. 155-156 ◽  
Author(s):  
MORRIS A. CYNKIN ◽  
GILBERT ASHWELL
Keyword(s):  
Thiobarbituric Acid ◽  
Acid Reaction ◽  
Deoxy Sugars

A Simple Method for Estimation of Lipoxygenase and Cyclo-Oxygenase Pathways in Human Platelets - the Use of Thiobarbituric Acid Reaction

1980 ◽  
Vol 44 (02) ◽  
pp. 111-114 ◽  
Author(s):  
Hiroshi Takayama ◽  
Minoru Okuma ◽  
Haruto Uchino
Keyword(s):  
Time Course ◽  
Normal Values ◽  
Human Platelet ◽  
Thiobarbituric Acid ◽  
Human Platelets ◽  
Optimal Conditions ◽  
Experimental Conditions ◽  
Simple Method ◽  
Acid Reaction ◽  
Optimal Ph

SummaryTo develop a simple method for estimation of platelet lipoxygenase (PLO) and cyclo-oxygenase (PCO) pathways, the arachidonic acid (AA) metabolism of human platelet was investigated under various experimental conditions by the use of the thiobarbituric acid (TBA) reaction and a radioisotope technique. A TBA-reactive substance different from malondialdehyde (MDA) via PCO pathway was detected and shown to be derived from the PLO pathway. Since the optimal pH and time course of its formation were different from those of MDA formation via PCO pathway, PLO and PCO pathways were estimated by quantitating the TBA-reactive substances produced by the incubation of AA either with aspirin-treated platelets or with untreated ones, respectively, each under optimal conditions. Normal values expressed in terms of nmol MDA/108 platelets were 1.17±0.34 (M±SD, n = 31) and 0.79±0.15 (n = 31) for PLO and PCO pathways, respectively.

Synthesis and biological activity of new 1,3,4-thiadiazole derivatives

2006 ◽  
Vol 60 (1) ◽  
Author(s):  
A. Aly ◽  
R. El-Sayed
Keyword(s):  
Biological Activity ◽  
Carbon Disulfide ◽  
Acetyl Chloride ◽  
Thioglycolic Acid ◽  
Thiobarbituric Acid ◽  
Aromatic Aldehydes ◽  
Phenyl Isocyanate ◽  
Phenyl Isothiocyanate ◽  
Acid Reaction ◽  
Thiadiazole Derivatives

AbstractThe aminothiadiazole (II) on treatment with aromatic aldehydes yielded Schiff bases, which cyclized to thiazolidinone derivatives by reaction with thioglycolic acid. Reaction of II with phenyl isocyanate and phenyl isothiocyanate afforded the carbamide and carbothiamide derivatives, respectively, which on reaction with malonic acid in acetyl chloride gave barbituric and thiobarbituric acid derivatives. However, reaction of carbon disulfide and methyl iodide with II gave dithiocarbamidate derivative which on treatment with ethylenediamine or o-phenylenediamine gave the condensed N-imidazolylthiadiazolylamine derivatives.

Determination of malonaldehyde in human plasma: elimination of spectral interferences in the 2-thiobarbituric acid reaction

The Analyst ◽  
1993 ◽  
Vol 118 (1) ◽  
pp. 89 ◽  
Author(s):  
Anunciaci�n Espinosa-Mansilla ◽  
Francisco Salinas ◽  
Amparo Rubio Leal
Keyword(s):  
Human Plasma ◽  
Thiobarbituric Acid ◽  
Spectral Interferences ◽  
Acid Reaction

scholarly journals Separation and characterization of the aldehydic products of lipid peroxidation stimulated by ADP-Fe2+ in rat liver microsomes

1982 ◽  
Vol 208 (1) ◽  
pp. 129-140 ◽  
Author(s):  
H Esterbauer ◽  
K H Cheeseman ◽  
M U Dianzani ◽  
G Poli ◽  
T F Slater
Keyword(s):  
Lipid Peroxidation ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Thiobarbituric Acid ◽  
Polar Fraction ◽  
Supernatant Fraction ◽  
Major Type ◽  
Rat Liver Microsomes ◽  
Acid Reaction ◽  
The Individual

1. Methods using t.l.c. and high-pressure liquid chromatography (h.p.l.c.) have been used to separate the complex variety of substances possessing a carbonyl function that are produced during lipid peroxidation. 2. The major type of lipid peroxidation studied was the ADP-Fe2+-stimulated peroxidation of rat liver microsomal phospholipids. Preliminary separation of the polar and non-polar products was achieved by t.l.c.: further separation and identification of individual components was performed by h.p.l.c. Estimations were performed on microsomal pellets and the supernatant mixture after incubation of microsomes for 30 min at 37 degrees C. 3. The polar fraction was larger than the non-polar fraction when expressed as nmol of carbonyl groups/g of liver. In the non-polar supernatant fraction the major contributors were n-alkanals (31% of the total), alpha-dicarbonyl compounds (22%) and 4-hydroxyalkenals (37%) with the extraction method used. 4. Major individual contributors to the non-polar fraction were found to be propanal, 4-hydroxynonenal, hexanal and oct-2-enal. Other components identified include butanal, pent-2-enal, hex-2-enal, hept-2-enal, 4-hydroxyoctenal and 4-hydroxyundecenal. The polar carbonyl fraction was less complex than the non-polar fraction, although the identities of the individual components have not yet been established. 5. Since these carbonyl compounds do not react significantly in the thiobarbituric acid reaction, which largely demonstrates the presence of malonaldehyde, it is concluded that considerable amounts of biologically reactive carbonyl derivatives are released in lipid peroxidation and yet may not be picked up by the thiobarbituric acid reaction.

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