THE INFLUENCE OF LOW TEMPERATURES ABOVE FREEZING UPON THE RATE OF AUTOLYTIC AND BACTERIAL DECOMPOSITION OF HADDOCK MUSCLE
The influence of temperature upon autolytic and bacterial decomposition of haddock muscle within the range of 36 to 30° F. (+2.2 to −1.1 °C.) has been followed by determining the increase in the amount of volatile basic nitrogen parallel with the increase in the number of bacteria. A mixed flora of bacteria normally occurring on haddock was used for inoculation purposes. The experimental results show that practically no autolysis takes place at these temperatures, and that the bacterial decomposition and bacterial growth rate at 30° F. is only about half of that at 36° F. Expressed in mathematical terms, it was found that the temperature coefficients (Q10) for these reactions during the phases of logarithmic increase were considerably higher than the values for Q10 for ordinary life processes at medium temperatures (Q10 = 2 to 3, van't Hoff). The values for Q10 increased rapidly with decreasing temperature range. The practical application of these findings has been demonstrated to be of economical importance in the holding of fresh fish at 30° F. instead of the customary holding in crushed ice at about 36° F.