Use of genetic markers to aid in re-establishing migratory connectivity in a fragmented metapopulation of bull trout (Salvelinus confluentus)

2011 ◽  
Vol 68 (11) ◽  
pp. 1952-1969 ◽  
Author(s):  
Patrick W. DeHaan ◽  
Shana R. Bernall ◽  
Joseph M. DosSantos ◽  
Lawrence L. Lockard ◽  
William R. Ardren

Dams and other barriers fragment important migratory corridors for bull trout ( Salvelinus confluentus ) across the species range. Three dams constructed without fish passage facilities prevented migratory bull trout in the Lake Pend Oreille and Clark Fork River system in Idaho and Montana, USA, from returning to their natal spawning tributaries for nearly 100 years. We genotyped bull trout from 39 spawning tributaries to assemble a baseline data set that we used to develop a real-time genotyping and analysis protocol to assist with upstream fish transport decisions. Self-assignment tests and analysis of blind samples indicated that unknown individuals could be assigned to their region of origin with a high degree of confidence. From 2004 to 2010, genetic assignments were conducted for 259 adult bull trout collected below mainstem dams. Based on genetic assignments, 203 fish were transported upstream above one or more dams. This protocol has helped re-establish connectivity in a fragmented system, providing increased numbers of spawning adults for numerically depressed populations above the dams. We discuss the utility of genetic data for assisting with upstream passage decisions.

2006 ◽  
Vol 53 (12) ◽  
pp. 247-256 ◽  
Author(s):  
A. Marquot ◽  
A.-E. Stricker ◽  
Y. Racault

Activated sludge models, and ASM1 in particular, are well recognised and useful mathematical representations of the macroscopic processes involved in the biological degradation of the pollution carried by wastewater. Nevertheless, the use of these models through simulation software requires a careful methodology for their calibration (determination of the model parameters' values) and the validation step (verification with an independent data set). This paper presents the methodology and the results of dynamic calibration and validation tasks as a prior work to a modelling project for defining a reference guideline destined to French designers and operators. To reach these goals, a biological nutrient removal (BNR) wastewater treatment plant (WWTP) with intermittent aeration was selected and monitored for 2 years. Two sets of calibrated parameters are given and discussed. The results of the long-term validation task are presented through a 2-month simulation with lots of operation changes. Finally, it is concluded that, even if calibrating ASM1 with a high degree of confidence with a single set of parameters was not possible, the results of the calibration are sufficient to obtain satisfactory results over long-term dynamic simulation. However, simulating long periods reveals specific calibration issues such as the variation of the nitrification capacity due to external events.


2016 ◽  
Vol 7 (1) ◽  
pp. 10-19 ◽  
Author(s):  
B. Scheuchl

Polarimetric SAR data provide information about the scattering of the area observed. The availability of data stacks allows the identification of stable targets and subsequent scattering analysis with a high degree of confidence at full resolution. A novel approach to find and evaluate polarimetric persistent target is presented, that is an extension of well-established analysis methods for single scenes. The use of the Cloude-Pottier distributed target decomposition analysis applied on the temporal averages (as opposed to spatial averaging), combined with a Cameron point target analysis applied on each layer separately to select pixels only, provides an efficient scattering classification of polarimetric persistent point targets in the stack. This method can also be used to analyze targets identified through other means, albeit at a lower degree of confidence. The approach retains the full resolution of the data set, though temporal changes between acquisitions add additional complexity. Result interpretation is therefore performed under consideration of a set of boundary conditions. Results from the analysis of two polarimetric data stacks acquired by RADARSAT-2 are shown.


2021 ◽  
Vol 9 ◽  
Author(s):  
Jeffrey J. Duda ◽  
Christian E. Torgersen ◽  
Samuel J. Brenkman ◽  
Roger J. Peters ◽  
Kathryn T. Sutton ◽  
...  

The removal of two large dams on the Elwha River was completed in 2014 with a goal of restoring anadromous salmonid populations. Using observations from ongoing field studies, we compiled a timeline of migratory fish passage upstream of each dam. We also used spatially continuous snorkeling surveys in consecutive years before (2007, 2008) and after (2018, 2019) dam removal during summer baseflow to assess changes in fish distribution and density over 65 km of the mainstem Elwha River. Before dam removal, anadromous fishes were limited to the 7.9 km section of river downstream of Elwha Dam, potamodromous species could not migrate throughout the river system, and resident trout were the most abundant species. After dam removal, there was rapid passage into areas upstream of Elwha Dam, with 8 anadromous species (Chinook, Coho, Sockeye, Pink, Chum, Winter Steelhead, Summer Steelhead, Pacific Lamprey, and Bull Trout) observed within 2.5 years. All of these runs except Chum Salmon were also observed in upper Elwha upstream of Glines Canyon Dam within 5 years. The spatial extent of fish passage by adult Chinook Salmon and Summer Steelhead increased by 50 km and 60 km, respectively, after dam removal. Adult Chinook Salmon densities in some previously inaccessible reaches in the middle section of the river exceeded the highest densities observed in the lower section of the river prior to dam removal. The large number (>100) of adult Summer Steelhead in the upper river after dam removal was notable because it was among the rarest anadromous species in the Elwha River prior to dam removal. The spatial extent of trout and Bull Trout remained unchanged after dam removal, but their total abundance increased and their highest densities shifted from the lower 25 km of the river to the upper 40 km. Our results show that reconnecting the Elwha River through dam removal provided fish access to portions of the watershed that had been blocked for nearly a century.


1993 ◽  
Vol 71 (2) ◽  
pp. 238-247 ◽  
Author(s):  
David B. Donald ◽  
David J. Alger

Indigenous lacustrine populations of bull trout (Salvelinus confluentus) and lake trout (S. namaycush) are spatially separated within the southern part of the zone of distributional overlap (northern Montana, southwestern Alberta, and east-central British Columbia). In this area, lake trout occurred primarily in mountain lakes of 1032–1500 m elevation, while bull trout were found primarily in lakes between 1500 and 2200 m. Introductions of lake trout in the twentieth century and data obtained from beyond the study area indicated that both fishes can establish significant allopatric populations (more than 5% of the catch) in large, deep lakes (>8 ha in area and >8 m deep) over a wide elevation range. We tested the hypothesis that lake trout displace or exclude bull trout from lakes by determining the outcome of introductions of lake trout into two lakes that supported indigenous bull trout. Lake trout were introduced into Bow Lake in 1964, and by 1992 the bull trout population was decimated there and in another lake (Hector) situated 15 km downstream. Thus, lake trout can displace bull trout and may prevent bull trout from becoming established in certain low-elevation lakes. Population age-structure analyses also suggest that lake trout adversely affected bull trout. Bull trout populations in sympatry with lake trout, including the one extirpated from Hector Lake, had few old fish (18% were more than 5 years old; N = 40 fish from three lakes) compared with allopatric populations (49% were more than 5 years old; N = 235 fish from seven lakes). Niche overlap and the potential for competition between the two char species were substantial. In lakes with trophic structure ranging from simple to complex, bull trout and lake trout fed on similar foods and had similar ecological efficiencies (growth rates). Predation by lake trout on bull trout was not documented during the study.


2018 ◽  
Vol 19 (12) ◽  
pp. 3780 ◽  
Author(s):  
Dingxuan He ◽  
Andrew Gichira ◽  
Zhizhong Li ◽  
John Nzei ◽  
Youhao Guo ◽  
...  

The order Nymphaeales, consisting of three families with a record of eight genera, has gained significant interest from botanists, probably due to its position as a basal angiosperm. The phylogenetic relationships within the order have been well studied; however, a few controversial nodes still remain in the Nymphaeaceae. The position of the Nuphar genus and the monophyly of the Nymphaeaceae family remain uncertain. This study adds to the increasing number of the completely sequenced plastid genomes of the Nymphaeales and applies a large chloroplast gene data set in reconstructing the intergeneric relationships within the Nymphaeaceae. Five complete chloroplast genomes were newly generated, including a first for the monotypic Euryale genus. Using a set of 66 protein-coding genes from the chloroplast genomes of 17 taxa, the phylogenetic position of Nuphar was determined and a monophyletic Nymphaeaceae family was obtained with convincing statistical support from both partitioned and unpartitioned data schemes. Although genomic comparative analyses revealed a high degree of synteny among the chloroplast genomes of the ancient angiosperms, key minor variations were evident, particularly in the contraction/expansion of the inverted-repeat regions and in RNA-editing events. Genome structure, and gene content and arrangement were highly conserved among the chloroplast genomes. The intergeneric relationships defined in this study are congruent with those inferred using morphological data.


Ecohydrology ◽  
2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Mark K. Taylor ◽  
Caleb T. Hasler ◽  
Scott G. Hinch ◽  
Bronwen Lewis ◽  
Dana C. Schmidt ◽  
...  

1997 ◽  
Vol 119 (1) ◽  
pp. 57-60
Author(s):  
S. Qin ◽  
G. E. O. Widera

When performing inservice inspection on a large volume of identical components, it becomes an almost impossible task to inspect all those in which defects may exist, even if their failure probabilities are known. As a result, an appropriate sample size needs to be determined when setting up an inspection program. In this paper, a probabilistic analysis method is employed to solve this problem. It is assumed that the characteristic data of components has a certain distribution which can be taken as known when the mean and standard deviations of serviceable and defective sets of components are estimated. The sample size can then be determined within an acceptable assigned error range. In this way, both false rejection and acceptance can be avoided with a high degree of confidence.


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