scholarly journals Antimicrobial nanomaterials against biofilms: an alternative strategy

2017 ◽  
Vol 25 (2) ◽  
pp. 225-244 ◽  
Author(s):  
Chunhua Liu ◽  
Jing Guo ◽  
Xiaoqing Yan ◽  
Yongbing Tang ◽  
Asit Mazumder ◽  
...  
Keyword(s):  
Public Health ◽  
Antimicrobial Agent ◽  
Environmental Impacts ◽  
Environmental Conditions ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Industrial Sectors ◽  
Antimicrobial Function ◽  
Antibiofilm Agents

Microbial adhesion to surfaces and the consequent biofilm formation under various environmental conditions is a common ecological phenomenon. Although biofilms play crucial beneficial roles in many processes, they can also cause serious problems for food, biomedical, environmental, and industrial sectors, leading to higher costs of production and equipment maintenance, and negative public health and environmental impacts. Biofilms are difficult to eradicate due to their resistance to conventional antimicrobial applications. Consequently, attention has been devoted to new emerging nanomaterials for their remarkable antimicrobial function. Understanding the inactivation mechanisms is the key to increase the efficiency of nanoparticles (NPs) and enhance the feasibility of their application against various microorganisms under different environments. In this paper, we review the activities of NPs as antimicrobial agents. We also discuss the mechanisms and factors contributing to antimicrobial properties of NPs. In addition, we describe some of the approaches employing NPs as effective antimicrobial agent, and associated challenges and problems in developing NPs as effective antibiofilm agents.

scholarly journals Chemically modified polystyrene co-loaded with antimicrobial agents

2021 ◽  
Vol 2120 (1) ◽  
pp. 012012
Author(s):  
Y X Koh ◽  
H L Choo ◽  
Y H Wong ◽  
C H Yeong
Keyword(s):  
Antimicrobial Agent ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Silver Ions ◽  
Exchange Capacity ◽  
Secondary Process ◽  
Ionic Exchange ◽  
Bacterial Strains ◽  
Surgical Guides ◽  
Antimicrobial Material

Abstract A recent study showed that at least 50% of nosocomial infections are due to medical indwelling devices like surgical guides and prosthetics. This amounts to about 2 million patients affected a year. The reason for such statistics is the growth of microorganisms on the surfaces of the medical devices. There have been many attempts to create antimicrobial materials but most materials are unable to hold more than one antimicrobial agent without a secondary process. The study related to antimicrobial material with more than one type of agent is rarely found in literature. Hence, the objective of this project is to produce an antimicrobial material that can hold more than one antimicrobial agent without the need for a secondary process. The material is produced by sulfonating high impact polystyrene (HIPS) and attaching copper and silver ions. The optimum time of sulfonation of the HIPS was determined by the degree of sulfonation and ion exchange capacity. Then, the sulfonated HIPS were loaded with both copper and silver ions at different ratios. The 6-hour sample yielded the highest degree of sulfonation and ionic exchange capacity of 33.7% and 2.57 meq/g, respectively. In future work, the characterization of the 6-hour sulfonated HIPS sample loaded with copper and silver ions at different concentration ratios will be performed using TGA, DSC and FTIR spectroscopy. Lastly, the efficacy of the antimicrobial properties of the sulfonated HIPS will be tested using different bacterial strains.

The versatile effect of L- and D-Cateslytin on bacteria and yeast biofilms according to configuration, medium and dose

2020 ◽  
Author(s):  
Lydie Ploux ◽  
Min Jin ◽  
Sophie Hellé ◽  
Cosette Betscha ◽  
Jean-Marc Strub ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
P Value ◽  
Microbial Cells ◽  
Response System ◽  
Stress Response System ◽  
Biofilm Development ◽  
The Impact

<p>L- and D-Cateslytin (CTL) are antimicrobial peptides (AMP) derived from chromogranin A, a protein of the stress response system. Their antimicrobial properties have been thoroughly characterized and already exploited in biomaterials. However, effects on biofilms of yeast and bacteria have never been specifically addressed. We have investigated the impact of both L and D configurations of CTL on the growth of biofilms formed by Candida albicans, Escherichia coli or Staphylococcus aureus microorganisms.</p> <p>The study was conducted in different media and two strategies of treatment were tested, consisting of administrating the peptide either just at the beginning of biofilm development i.e. on just adhering pioneer microbial cells or on a biofilm already allowed to develop for 24h. We also considered whether the peptide was modified in contact with the medium or/and microbial metabolites. Planktonic and sessile populations of microbial cells were analyzed by spectrophotometry, crystal violet staining, MTT and confocal microscopy with staining by Syto9Ò and propidium iodide. Identification of the peptides and their derived fragments was investigated by HPLC and Mass-Spectroscopy.</p> <p>In general, CTL-D exhibited higher antibiofilm performances than CTL-L. In addition, concentrations necessary to inhibit biofilm formation were found to vary from ten to eighty times the MICs determined in planktonic cultures. Nevertheless, the results also demonstrate that sessile microorganisms and biofilms are sensitive to CTL (L and D conformations) differently that planktonic populations. Significant (p-value < 0.01) effects were observed on both sessile and planktonic populations and with both strategies of treatments, but they highly varied with medium, species and CTL configuration. Typically, better antibiofilm effect than common antibiotics was reached in some specific conditions, while enhancement of aggregation or biofilm formation occurred in another medium and for other doses. Nevertheless,</p> <p>Finally, this confirms the quality of CTL peptides as new antimicrobial agents and reveals their anti-biofilm properties. This also specifies the conditions of use necessary to benefit of the highest performances.</p>

scholarly journals Synthesis and Evaluation of Saccharide-Based Aliphatic and Aromatic Esters as Antimicrobial and Antibiofilm Agents

2019 ◽  
Vol 12 (4) ◽  
pp. 186 ◽  
Author(s):  
Raffaella Campana ◽  
Alessio Merli ◽  
Michele Verboni ◽  
Francesca Biondo ◽  
Gianfranco Favi ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Antibiofilm Activity ◽  
Preliminary Screening ◽  
E Coli ◽  
Aromatic Esters ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Food Applications ◽  
Antibiofilm Agents

A small library of sugar-based (i.e., glucose, mannose and lactose) monoesters containing hydrophobic aliphatic or aromatic tails were synthesized and tested. The antimicrobial activity of the compounds against a target panel of Gram-positive, Gram-negative and fungi was assessed. Based on this preliminary screening, the antibiofilm activity of the most promising molecules was evaluated at different development times of selected food-borne pathogens (E. coli, L. monocytogenes, S. aureus, S. enteritidis). The antibiofilm activity during biofilm formation resulted in the following: mannose C10 > lactose biphenylacetate > glucose C10 > lactose C10. Among them, mannose C10 and lactose biphenylacetate showed an inhibition for E. coli 97% and 92%, respectively. At MICs values, no toxicity was observed on Caco-2 cell line for all the examined compounds. Overall, based on these results, all the sugar-based monoesters showed an interesting profile as safe antimicrobial agents. In particular, mannose C10 and lactose biphenylacetate are the most promising as possible biocompatible and safe preservatives for pharmaceutical and food applications.

scholarly journals Utilization of Antibacterial Nanoparticles in Photocurable Additive Manufacturing of Advanced Composites for Improved Public Health

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2616
Author(s):  
Christopher Billings ◽  
Changjie Cai ◽  
Yingtao Liu
Keyword(s):  
Public Health ◽  
Tensile Strength ◽  
Additive Manufacturing ◽  
Abrasion Resistance ◽  
Antimicrobial Properties ◽  
Digital Light Processing ◽  
Antimicrobial Function ◽  
3D Printed ◽  
Commercial Applications ◽  
Antibacterial Nanoparticles

This paper presents the additive manufacturing and characterization of nanoparticle-reinforced photocurable resin-based nanocomposites with a potential antimicrobial function for improved public health applications. Two types of photocurable resins are reinforced by titanium dioxide (TiO2) or zinc oxide (ZnO) nanoparticles with average diameters in the 10–30 nm range to provide antimicrobial properties. The developed nanocomposites can be additively manufactured using the digital light processing method with an outstanding surface quality and precise geometrical accuracy. Experimental characterizations are conducted to investigate key mechanical properties of the 3D printed nanocomposites, including Young’s Modulus, tensile strength, and abrasion resistance. Specimens produced were observed to demonstrate the following characteristics during testing. Tensile strength increased by 42.2% at a maximum value of 29.53 MPa. The modulus of elasticity increased by 14.3%, and abrasion resistance increased by 15.8%. The proper dispersion of the nanoparticles within the cured resin is validated by scanning electron images. The wettability and water absorption testing results indicate that the developed nanocomposites have an outstanding water resistance capability. The pairing of digital light processing with these novel nanocomposites allows for the creation of complex composite geometries that are not capable through other manufacturing processes. Therefore, they have the potential for long-term usage to improve general public health with antimicrobial functionality. The pairing of an unmodified photocurable resin with a 1% ZnO concentration demonstrated the most promise for commercial applications.

scholarly journals Antimicrobial Hexaaquacopper(II) Complexes with Novel Polyiodide Chains

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1005
Author(s):  
Zehra Edis ◽  
Radhika Raheja ◽  
Samir Haj Bloukh ◽  
Richie R. Bhandareh ◽  
Hamid Abu Sara ◽  
...  
Keyword(s):  
Antimicrobial Agent ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Stable Complex ◽  
In Vitro Synthesis ◽  
Iodine Release ◽  
Chain Type

The non-toxic inorganic antimicrobial agents iodine (I2) and copper (Cu) are interesting alternatives for biocidal applications. Iodine is broad-spectrum antimicrobial agent but its use is overshadowed by compound instability, uncontrolled iodine release and short-term effectiveness. These disadvantages can be reduced by forming complex-stabilized, polymeric polyiodides. In a facile, in-vitro synthesis we prepared the copper-pentaiodide complex [Cu(H2O)6(12-crown-4)5]I6 ´ 2I2, investigated its structure and antimicrobial properties. The chemical structure of the compound has been verified. We used agar well and disc-diffusion method assays against nine microbial reference strains in comparison to common antibiotics. The stable complex revealed excellent inhibition zones against C. albicans WDCM 00054, and strong antibacterial activities against several pathogens. [Cu(H2O)6(12-crown-4)5]I6 ´ 2I2 is a strong antimicrobial agent with an interesting crystal structure consisting of complexes located on an inversion center and surrounded by six 12-crown-4 molecules forming a cationic substructure. The six 12-crown-4 molecules form hydrogen bonds with the central Cu(H2O)6 . The anionic substructure is a halogen bonded polymer which is formed by formal I5− repetition units. The topology of this chain-type polyiodide is unique. The I5− repetition units can be understood as a triodide anion connected to two iodine molecules.

scholarly journals Effect of Farnesol on Staphylococcus aureus Biofilm Formation and Antimicrobial Susceptibility

2006 ◽  
Vol 50 (4) ◽  
pp. 1463-1469 ◽  
Author(s):  
M. A. Jabra-Rizk ◽  
T. F. Meiller ◽  
C. E. James ◽  
M. E. Shirtliff
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Membrane Integrity ◽  
Antimicrobial Properties ◽  
Bloodstream Infections ◽  
High Concentration ◽  
Bacterial Populations ◽  
Cell Membrane Integrity ◽  
Plate Counts

ABSTRACT Staphylococcus aureus is among the leading pathogens causing bloodstream infections able to form biofilms on host tissue and indwelling medical devices and to persist and cause disease. Infections caused by S. aureus are becoming more difficult to treat because of increasing resistance to antibiotics. In a biofilm environment particularly, microbes exhibit enhanced resistance to antimicrobial agents. Recently, farnesol was described as a quorum-sensing molecule with possible antimicrobial properties. In this study, the effect of farnesol on methicillin-resistant and -susceptible strains of S. aureus was investigated. With viability assays, biofilm formation assessment, and ethidium bromide uptake testing, farnesol was shown to inhibit biofilm formation and compromise cell membrane integrity. The ability of farnesol to sensitize S. aureus to antimicrobials was assessed by agar disk diffusion and broth microdilution methods. For both strains of staphylococci, farnesol was only able to reverse resistance at a high concentration (150 μM). However, it was very successful at enhancing the antimicrobial efficacy of all of the antibiotics to which the strains were somewhat susceptible. Therefore, synergy testing of farnesol and gentamicin was performed with static biofilms exposed to various concentrations of both agents. Plate counts of harvested biofilm cells at 0, 4, and 24 h posttreatment indicated that the combined effect of gentamicin at 2.5 times the MIC and farnesol at 100 μM (22 μg/ml) was able to reduce bacterial populations by more than 2 log units, demonstrating synergy between the two antimicrobial agents. This observed sensitization of resistant strains to antimicrobials and the observed synergistic effect with gentamicin indicate a potential application for farnesol as an adjuvant therapeutic agent for the prevention of biofilm-related infections and promotion of drug resistance reversal.

scholarly journals Potential and Prophylactic Use of Plants Containing Saponin-Type Compounds as Antibiofilm Agents against Respiratory Tract Infections

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
I. Irem Tatli Cankaya ◽  
E. Inci Somuncuoglu
Keyword(s):  
Respiratory Tract ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Antibiofilm Activity ◽  
Upper Respiratory Tract ◽  
Lung Infections ◽  
Prophylactic Use ◽  
Tract Infections ◽  
Antibiofilm Agents

Epidemic diseases have been observed in every period of human history, and the treatment process has taken time. Causative microorganisms reproduce as biofilm and contribute to the emergence of various infectious diseases. The process that starts with respiratory disorders causes serious lung infections due to bacteria and viruses that accumulate and multiply. The biofilms are difficult to eliminate and show increased resistance to available antimicrobial agents. There is a need to identify and develop potential resources used in treatment. The search for novel biological agents from plants is gaining popularity due to the high abundance, accessibility with consequent lower cost for discovery, and lesser side effects and toxicity. Saponins found in some plants can be alternative to antibiotics, with antimicrobial activities. This review focused on the potency of saponin-containing plants with antimicrobial properties as antibiofilm agents against these infections. For this purpose, keywords were scanned in Web of Science, Scopus, and Google academics databases, and the related literature was compiled. Approximately, 25 plant taxa belonging to 18 families traditionally used in the treatment of respiratory diseases are listed. These taxa mostly belong to Fabaceae, Asteraceae, Apiaceae, and Asparagaceae families, respectively. Most of these taxa have antibacterial, antifungal, antitussive, and anti-inflammatory activities. Especially, plants with antibiofilm activity that can be effective against many microorganisms are compiled in this study. These plants can prevent or treat upper respiratory tract diseases caused by bacteria due to the phytochemicals they contain, especially saponins.

scholarly journals Antimicrobial activity of a novel Spanish propolis against planktonic and sessile oral Streptococcus spp

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Luisa Navarro-Pérez ◽  
Virginia Vadillo-Rodríguez ◽  
Irene Fernández-Babiano ◽  
Ciro Pérez-Giraldo ◽  
M. Coronada Fernández-Calderón
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Oral Streptococcus ◽  
Ancient Times ◽  
Ethanolic Extract Of Propolis

AbstractIncreased bacterial resistance to traditional antimicrobial agents has prompted the use of natural products with antimicrobial properties such as propolis, extensively employed since ancient times. However, the chemical composition of propolis extracts is extremely complex and has been shown to vary depending on the region and season of collection, due to variations in the flora from which the pharmacological substances are obtained, being therefore essential for their antimicrobial activity to be checked before use. For this purpose, we evaluate the in vitro antimicrobial and anti-biofilm activity of a new and promising Spanish ethanolic extract of propolis (SEEP) on Streptococcus mutans and Streptococcus sanguinis, responsible, as dominant ‘pioneer’ species, for dental plaque. Results reveal that S. sanguinis is more sensitive to SEEP, slowing and retarding its growth considerably with lower concentrations than those needed to produce the same effect in S. mutans. SEEP presents concentration- and time-dependent killing activity and, furthermore, some of the subinhibitory concentrations employed increased biofilm formation even when bacterial growth decreased. Mono and dual-species biofilms were also inhibited by SEEP. Findings obtained clearly show the relevance of using biofilm and subinhibitory concentration models to determine optimal treatment concentrations.

scholarly journals Antibiotic and Antibiofilm Activities of Salvadora persica L. Essential Oils against Streptococcus mutans: A Detailed Comparative Study with Chlorhexidine Digluconate

Pathogens ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 66 ◽  
Author(s):  
Merajuddin Khan ◽  
Hamad Z. Alkhathlan ◽  
Shams Tabrez Khan
Keyword(s):  
Antimicrobial Agent ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Oral Hygiene ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Antibiofilm Activity ◽  
Oil Well ◽  
Chlorhexidine Digluconate ◽  
Salvadora Persica

The use of organic components from plants as an alternative antimicrobial agent is becoming popular due to the development of drug-resistance in various pathogens. Essential oils from fresh (MF-1) and dried (MD-1) roots of Salvadora persica L. were extracted and benzyl isothiocynate was determined as their chief constituent using GC-MS and GC-FID. The antibiofilm and antimicrobial activities of MD-1 and MF-1 against Streptococcus mutans a dental caries causing bacteria were determined using multiple assays. These activities were compared with chlorhexidine digluconate (CHX) and clove oil, well known antimicrobial agents for oral hygiene. Essential oils demonstrated IC50 values (10–11 µg/mL) comparable to that of CHX, showed a significant reduction (82 ± 7–87 ± 6%) of the biofilm formation at a very low concentration. These results were supported by RT-PCR studies showing change in the expression levels of AtlE, gtfB, ymcA and sodA genes involved in autolysis, biofilm formation and oxidative stress, respectively. The results presented in this study show the robust bactericidal and antibiofilm activity of MD-1 and MF-1 against S. mutans which is comparable to Chlorhexidine digluconate. Our results suggest that these essential oils can be as effective as CHX and hence can serve as a good alternative antimicrobial agent for oral hygiene.

scholarly journals Antimicrobial agents from Malaysian plants and their potential use in food packaging material: Review

2017 ◽  
Vol 19 ◽  
pp. 57 ◽  
Author(s):  
FA Mustapha ◽  
J Jai ◽  
F Hamidon ◽  
ZI Md Sharif ◽  
N Mohd Yusof
Keyword(s):  
Antimicrobial Agent ◽  
Food Packaging ◽  
Chemical Engineering ◽  
Antimicrobial Agents ◽  
Defense Mechanism ◽  
Antimicrobial Properties ◽  
Edible Films ◽  
Main Role ◽  
Engineering Research ◽  
Medicinal Properties

<p>Malaysia is among 12 countries in the world that rich in biodiversity including an assortment of plants with potential sources for new antimicrobial agents. Despite the fact that various plants have been screened, the requirement for detail study on antimicrobial compounds from plants is preceded as safer and better agent to inhibit growth of microbes. Therefore, selected Malaysia plants with medicinal properties are listed for further review in their antimicrobial activity and their major compound that act as antimicrobial agent. The major groups of the antimicrobial constituents are phenolics, phenolic acids, quinones, saponins, flavonoids, tannins, coumarins, terpenoids and alkaloids. These compounds are secondary metabolites that play the main role in plant defense mechanism. They also exhibited inhibitory effect on various microorganisms such as <em>Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli </em>and <em>Candida albicans</em>. Compounds derived from Malaysian plants have the potential to be used as antimicrobial additive as most of their extracts containing active compounds such as caffeic acid, pyrogallol, catechin and curcumin. Extraction method of plants extract is done either by conventional method of maceration and extraction under reflux and steam distillation or modern method of microwave assisted extraction, supercritical fluid extraction and ultrasound-assisted solvent extraction. Even though plants extracts with medicinal properties are gaining fame for their antimicrobial properties, however the study on incorporation of the extracts into edible films as antimicrobial food packaging is limited. The advantages of using an edible film with antimicrobial agent plants for food products are it safe to use and it able to extend the shelf life while reducing packaging waste.</p><p>Chemical Engineering Research Bulletin 19(2017) 57-66</p><strong></strong>

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