PASTURE STUDIES. XIX.: A SIMPLIFIED APPARATUS FOR THE CONTINUOUS EXTRACTION OF MOISTURE AND FAT FROM BIOLOGICAL MATERIALS

1941 ◽  
Vol 19b (4) ◽  
pp. 116-121
Author(s):  
E. W. Crampton ◽  
T. L. Purdy
Keyword(s):  
Ethyl Ether ◽  
Plant Material ◽  
Biological Materials ◽  
Isopropyl Ether ◽  
Continuous Extraction ◽  
Oven Drying ◽  
Ether Extraction ◽  
Ether Yield

A modification of the Kaye apparatus for the determination of moisture by distillation and of lipids by isopropyl ether extraction is described. The modified apparatus has been used in the analysis of plant material and faeces, and typical results are presented. It appears that isopropyl ether and ethyl ether yield similar amounts of extract, but that oven-drying may result in values that are too low.

Extraction and Gas-Liquid Chromatographic Determination of Hexachlorophene from Several Plant Tissues

1977 ◽  
Vol 60 (5) ◽  
pp. 1081-1086
Author(s):  
Oscar W Van Auken ◽  
Michael Hulse
Keyword(s):  
Detection Limit ◽  
Ethyl Ether ◽  
Plant Tissue ◽  
Plant Material ◽  
Chromatographic Determination ◽  
Plant Tissues ◽  
Green Beans ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Conditions are described for extraction, cleanup, derivatization, detection, and quantitation of hexachlorophene (HCP) residues from several types of plant material. Wet plant tissue was homogenized and extracted with ethyl ether, dried, and methylated with excess diazomethane. Samples were precleaned by column chromatography on silica gel with benzene-petroleum ether (1 + 1), and then 1–10 μI concentrated sample was chromatographed on a 6′ column of 3% SE-30 on 80–100 mesh Gas-Chrom Q. The detection limit for the electron capture detector was <0.1 ng dimethoxyhexachlorophene and 1 ppb HCP in plant tissue. Recoveries (%) of 11–610 pph HCP added to tissue averaged 94.3 for tomatoes, 86.4 for green peppers, 92.6 for cucumbers, 93.3 for green beans, 93.7 for peanut hay, and 91.6 for peanut shells.

Enhanced information from biological materials through technological improvements in cryo-electron microscopy

1992 ◽  
Vol 50 (1) ◽  
pp. 788-789
Author(s):  
Marc J.C. de Jong ◽  
Wim M. Busing ◽  
Max T. Otten
Keyword(s):  
Electron Microscopy ◽  
Electron Beam ◽  
Biological Materials ◽  
Electron Crystallography ◽  
Cryo Electron Microscopy ◽  
Transmission Electron ◽  
The Many ◽  
Technological Improvements ◽  
Beam Damage

Biological materials damage rapidly in the electron beam, limiting the amount of information that can be obtained in the transmission electron microscope. The discovery that observation at cryo temperatures strongly reduces beam damage (in addition to making it unnecessaiy to use chemical fixatives, dehydration agents and stains, which introduce artefacts) has given an important step forward to preserving the ‘live’ situation and makes it possible to study the relation between function, chemical composition and morphology.Among the many cryo-applications, the most challenging is perhaps the determination of the atomic structure. Henderson and co-workers were able to determine the structure of the purple membrane by electron crystallography, providing an understanding of the membrane's working as a proton pump. As far as understood at present, the main stumbling block in achieving high resolution appears to be a random movement of atoms or molecules in the specimen within a fraction of a second after exposure to the electron beam, which destroys the highest-resolution detail sought.

Contribution to the Optimization of a Gas Chromatographic Method by QbD Approach used for Analysis of Essential Oils from Salvia officinalis

2019 ◽  
Vol 70 (6) ◽  
pp. 2015-2020
Author(s):  
Silvia Robu ◽  
Aurelia Romila ◽  
Olimpia Dumitriu Buzia ◽  
Adrian Florin Spac ◽  
Camelia Diaconu ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Plant Material ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Salvia Officinalis ◽  
Salvia Officinalis L ◽  
Gas Chromatographic Method

Numerous articles on Salvia officinalis L. have been published regarding the composition of their essential oil. The considerable variation found may be due to the quality of the plant material as well as to the methods used for analysis. A simple GC-MS method was developed and optimized in the QbD approach, for the determination of sage essential oils. The optimization of GC-MS analysis was performed using different mobile phase flows, injection volumes, split ratios and temperature programs. The optimized method proved to be simple and can be successfully applied for the determination of sage essential oils.

scholarly journals THE COLORIMETRIC DETERMINATION OF FRUCTOSAN IN PLANT MATERIAL

1945 ◽  
Vol 157 (1) ◽  
pp. 161-167
Author(s):  
Willard L. McRary ◽  
Marion C. Slattery
Keyword(s):  
Plant Material ◽  
Colorimetric Determination
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