Animal and vegetable fats and oils. Determination of unsaponifiable matter. Method using diethyl ether extraction

2001 ◽  
Keyword(s):  
Unsaponifiable Matter ◽  
Diethyl Ether ◽  
Fats And Oils ◽  
Ether Extraction

Thin Layer and Gas-Liquid Chromatography of Cholesterol in Fats and Oils. II. Collaborative Study

1969 ◽  
Vol 52 (4) ◽  
pp. 778-781
Author(s):  
C W Thorpe
Keyword(s):  
Unsaponifiable Matter ◽  
Collaborative Study ◽  
Fats And Oils ◽  
Gas Liquid Chromatography ◽  
Animal Fats ◽  
Animal Fat ◽  
Low Levels ◽  
Positive Results ◽  
Column Chromatographic

Abstract A method employing TLC-GLC for detecting animal fats in vegetable fats was subjected to collaborative study. The method involves preparative TLC of unsaponifiable matter followed by GLC of the isolated sterols. The results of this study, based on GLC determination of cholesterol in the isolated sterols, indicate that low levels (2.5%) of animal fat can be detected. The possibility of obtaining false positive results is greatly reduced compared with direct GLC of unsaponifiable matter or column chromatographic fractionation of unsaponifiable matter followed by GLC analysis.

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