THE RELATIONSHIP OF DIETARY FACTORS TO RAT SERUM ALKALINE PHOSPHATASE

1952 ◽  
Vol 30 (1) ◽  
pp. 18-25
Author(s):  
Jules Tuba ◽  
Neil B. Madsen
Keyword(s):  
Alkaline Phosphatase ◽  
Food Consumption ◽  
Serum Alkaline Phosphatase ◽  
Good Condition ◽  
Diabetic Rats ◽  
Dietary Factors ◽  
Adult Rats ◽  
Rat Serum ◽  
Level 3 ◽  
Relationship Of

Food consumption in alloxan diabetic rats shows a positive correlation with (1) alkaline phosphatase activity, (2) blood glucose level, (3) body weight, similar to the correlation between enzyme activity and food intake in normal adult rats when they are fed a stock laboratory diet. Oral vitamin B12 has no effect on serum alkaline phosphatase levels of normal or diabetic rats, nor does it modify the effect of supplementary methionine in either group. The effect of supplementary choline on the phosphatase of normal rats is unaltered by this vitamin which is reported to have transmethylating and lipotropic influences. Methionine added to animal checkers lowers phosphatase levels in both normal and diabetic adult rats, but this is directly related to lowered food consumption which always accompanies supplementation with this amino acid. The animals maintain their weights and appear to be in good condition, so it may be assumed that the lowered intake of food is adequate. Supplementary choline decreases phosphatase levels of normal rats by about 20% of initial values, and this appears to be due to choline itself and not to altered food consumption. Phosphatase levels of alloxan diabetic rats, however, are slightly but significantly elevated by supplementary choline.

THE RELATIONSHIP OF DIETARY FACTORS TO RAT SERUM ALKALINE PHOSPHATASE: 1. THE EFFECT OF FAT, METHIONINE, AND CYSTINE

1950 ◽  
Vol 28e (2) ◽  
pp. 41-46 ◽  
Author(s):  
Jules Tuba ◽  
Ridley K. Shaw
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Dietary Factors ◽  
Rat Serum ◽  
Serum Alkaline Phosphatase Activity ◽  
Weight Losses ◽  
Weanling Rats ◽  
Standard Laboratory Diet ◽  
Relationship Of

In synthetic diets fed to weanling rats, methionine and fat must be present in a definite ratio in order to maintain a serum alkaline phosphatase activity equal to that obtained on a standard laboratory diet of animal checkers. This ratio is approximately 1:25 by weight for a diet containing 8.5% fat. Increased fat enhances, while increased methionine lowers, the serum phosphatase activity. Although in some experiments methionine was fed in concentrations sufficient to lower phosphatase activity to what has been considered definitely subnormal values, growth was good and the general condition of the animals was excellent. However, beyond certain concentrations of the amino acid, food consumption decreased and weight losses occurred. Cystine had no effect in opposing the action of methionine on serum alkaline phosphatase.

THE RELATIONSHIP OF DIETARY FACTORS TO RAT SERUM ALKALINE PHOSPHATASE

1952 ◽  
Vol 30 (6) ◽  
pp. 515-519
Author(s):  
Jules Tuba ◽  
Kazie A. Siluch ◽  
Margaret I. Robinson ◽  
Neil B. Madsen
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Dietary Factors ◽  
Sodium Oxalate ◽  
Rat Serum ◽  
Growing Rats ◽  
Calcium And Magnesium ◽  
Relationship Of ◽  
The Relationship ◽  
Normal Calcium

Increased levels of serum alkaline phosphatase were produced in growing rats by diets low in calcium or by the addition of sodium oxalate or rhubarb to diets containing adequate amounts of calcium. In addition, variations from normal in levels of serum phosphorus, calcium, and magnesium, as well as the ash of the tibiae, indicate that the animals were rachitic. The amount of calcium retained in the bodies of animals maintained on a diet containing rhubarb, which has a high oxalate content, were very much lower than in growing rats fed a normal calcium diet.

THE EFFECTS OF SOME DIETARY DERIVED LIPIDS AND FAT SOLUBLE VITAMINS ON RAT SERUM TRIBUTYRINASE AND ALKALINE PHOSPHATASE

1952 ◽  
Vol 30 (4) ◽  
pp. 308-313
Author(s):  
Jack D. Taylor ◽  
Neil B. Madsen ◽  
Jules Tuba
Keyword(s):  
Fatty Acids ◽  
Alkaline Phosphatase ◽  
Oleic Acid ◽  
Stearic Acid ◽  
Serum Alkaline Phosphatase ◽  
Adult Rats ◽  
Rat Serum ◽  
Low Levels ◽  
Fat Soluble Vitamins ◽  
Increased Activity

Synthetic diets were fed to adult rats for four weeks to determine the effects of dietary stearic acid, oleic acid, glycerol, Crisco, and vitamins, A, D, and E on the activity of serum alkaline phosphatase and serum tributyrinase. On a diet devoid of fats or fatty acids, the rats manifested abnormally low enzyme levels, which for serum alkaline phosphatase fell to values characteristic of starvation. Basal levels of the two enzymes, obtained with a fat free diet, were not altered by the ingestion of glycerol or vitamins A, D, and E. Dietary stearic acid, oleic acid, and Crisco, each significantly increased activity of phosphatase and tributyrinase and it would appear that both enzymes are concerned with intestinal absorption of fatty acids. The effect of oleic acid was most pronounced with both enzymes. The rats all gained weight during the tests so none of the variations in enzyme levels can be attributed to inanition. After the dietary test periods, all groups were starved for one week. Serum phosphatase values fell to the same constant low levels for all animals. Tributyrinase values rose towards levels which suggest that the enzyme is concerned with mobilization of depot fats during periods of fasting.

THE RELATIONSHIP OF DIETARY FACTORS TO RAT SERUM ALKALINE PHOSPHATASE

1952 ◽  
Vol 30 (5) ◽  
pp. 378-382
Author(s):  
Jules Tuba ◽  
Bohdan Jelinek ◽  
Ridley K. Shaw ◽  
Neil B. Madsen
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Wheat Gluten ◽  
Dietary Factors ◽  
Male Rats ◽  
Brewer’S Yeast ◽  
Daily Consumption ◽  
Brewer's Yeast

Weanling rats were fed dietary concentrations of 0%, 5%, 10%, 30%, and 91% casein. Complete absence of dietary protein resulted in death of the young animals within five weeks. Activity of serum alkaline phosphatase was at starvation levels during this period. Optimal growth was obtained at 30% dietary level of casein, and alkaline phosphatase activity was in the normal range. No effect on the enzyme could be attributed to the protein. There is a highly significant correlation between daily food consumption and serum phosphatase activity, which is attributed to the fat component of the various diets. Adult male rats were subjected to a three-week period of protein depletion, which was followed by a two-week period of protein repletion. The protein was supplied in the following forms: casein, dried brewer’s yeast, wheat gluten, casein plus wheat gluten, and dried brewer’s yeast plus gluten. Enzyme values at the end of the experiment were typical for the high fat diet (26%) which these animals were receiving. Once more there is a significant correlation between serum alkaline phosphatase activity and daily consumption of fat, whereas the protein component of the diet is without effect on the enzyme.

scholarly journals Evalution of Anti-Diabetic Potential of Aqueous Extract of “Luffa cylindrica” (Native Sponge/Sponge Gourd) Leaf and Seed on Alloxan Induced Diabetic Wistar Rats

2019 ◽  
pp. 1-8
Author(s):  
Luka, C. D. Abigail ◽  
E. A. Metuaghan
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Normal Control ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Serum Alkaline Phosphatase ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Luffa Cylindrica ◽  
Leaf Extracts

The study was carried out to evaluate the anti-diabetic effect of Luffa cylindrical (native sponge /sponge gourd) seed and leaf extracts in alloxan- induced diabetic rats. Sixteen experimental rats were divided into four groups of four rats each: a, diabetic control; b, normal control; c, diabetic rats treated with seed extract (400 mg/kg) and d, diabetic rats treated with leaf extract (400 mg/kg). The groups A, C and D rats were induced with diabetes intraperitoneally with alloxan (150 mg/kg bw). Phytochemical screening was carried out on the plant seed and leaf extracts and the following biochemical tests were carried out: blood glucose, serum lipid profile, serum alanine aminotransferase, serum aspartate aminotransferase, serum alkaline phosphatase, total protein, albumin, creatinine, urea, uric acid and some electrolytes like Na+, K+, HCO3-, and Cl- the administration of alloxan to experimental rats resulted in an increased level of most biochemical parameters; blood glucose, serum alanine aminotransferase, serum aspartate aminotransferase and serum alkaline phosphatase, serum total cholesterol, triglyceride, low density lipoprotein, creatinine, urea and uric acid. Luffa cylindrica seed and leaf extracts was administered to groups c and d diabetic rats respectively for two weeks, results were compared with normal control and diabetic control rats these parameters were found to be significantly (p<0.05) high in the diabetic groups than in the normal control groups. Treatment with the plant extract significantly (p<0.05) reduced elevated blood levels of glucose, cholesterol, triglyceride, alkaline phosphatase, amylase, aspartate aminotransferase, alanine aminotransferase, creatinine, urea, uric acid associated with alloxan-induced diabetic rats. The plant tested positive for alkaloids, flavonoids, saponins and tannins, negative for cardiac glycosides, phenols, resins, terpenes and steroids. Extracts of Luffa cylindrica seed and leaf has shown to have anti-diabetic and anti-lipidemic effects generally on alloxan induced diabetic rats. The study’s findings has shown that the plant possess hypoglycaemic and hypolipidaemic property and has supported the traditional use of Luffa cylindrica plant in the management of diabetes and its complications.

Relationship of plasma tartrate resistant acid phosphatase to the bone isoenzyme of serum alkaline phosphatase in hyperparathyroidism

1983 ◽  
Vol 133 (2) ◽  
pp. 189-200 ◽  
Author(s):  
J.J. Štěpán ◽  
E. Šilinková-Málková ◽  
T. Havránek ◽  
J. Formánková ◽  
M. Zichová ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Tartrate Resistant Acid Phosphatase ◽  
Relationship Of
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