THE RELATIONSHIP OF DIETARY FACTORS TO RAT SERUM ALKALINE PHOSPHATASE: 1. THE EFFECT OF FAT, METHIONINE, AND CYSTINE

1950 ◽  
Vol 28e (2) ◽  
pp. 41-46 ◽  
Author(s):  
Jules Tuba ◽  
Ridley K. Shaw
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Dietary Factors ◽  
Rat Serum ◽  
Serum Alkaline Phosphatase Activity ◽  
Weight Losses ◽  
Weanling Rats ◽  
Standard Laboratory Diet ◽  
Relationship Of

In synthetic diets fed to weanling rats, methionine and fat must be present in a definite ratio in order to maintain a serum alkaline phosphatase activity equal to that obtained on a standard laboratory diet of animal checkers. This ratio is approximately 1:25 by weight for a diet containing 8.5% fat. Increased fat enhances, while increased methionine lowers, the serum phosphatase activity. Although in some experiments methionine was fed in concentrations sufficient to lower phosphatase activity to what has been considered definitely subnormal values, growth was good and the general condition of the animals was excellent. However, beyond certain concentrations of the amino acid, food consumption decreased and weight losses occurred. Cystine had no effect in opposing the action of methionine on serum alkaline phosphatase.

THE RELATIONSHIP OF DIETARY FACTORS TO RAT SERUM ALKALINE PHOSPHATASE

1952 ◽  
Vol 30 (1) ◽  
pp. 18-25
Author(s):  
Jules Tuba ◽  
Neil B. Madsen
Keyword(s):  
Alkaline Phosphatase ◽  
Food Consumption ◽  
Serum Alkaline Phosphatase ◽  
Good Condition ◽  
Diabetic Rats ◽  
Dietary Factors ◽  
Adult Rats ◽  
Rat Serum ◽  
Level 3 ◽  
Relationship Of

Food consumption in alloxan diabetic rats shows a positive correlation with (1) alkaline phosphatase activity, (2) blood glucose level, (3) body weight, similar to the correlation between enzyme activity and food intake in normal adult rats when they are fed a stock laboratory diet. Oral vitamin B12 has no effect on serum alkaline phosphatase levels of normal or diabetic rats, nor does it modify the effect of supplementary methionine in either group. The effect of supplementary choline on the phosphatase of normal rats is unaltered by this vitamin which is reported to have transmethylating and lipotropic influences. Methionine added to animal checkers lowers phosphatase levels in both normal and diabetic adult rats, but this is directly related to lowered food consumption which always accompanies supplementation with this amino acid. The animals maintain their weights and appear to be in good condition, so it may be assumed that the lowered intake of food is adequate. Supplementary choline decreases phosphatase levels of normal rats by about 20% of initial values, and this appears to be due to choline itself and not to altered food consumption. Phosphatase levels of alloxan diabetic rats, however, are slightly but significantly elevated by supplementary choline.

THE RELATIONSHIP OF DIETARY FACTORS TO RAT SERUM ALKALINE PHOSPHATASE

1952 ◽  
Vol 30 (6) ◽  
pp. 515-519
Author(s):  
Jules Tuba ◽  
Kazie A. Siluch ◽  
Margaret I. Robinson ◽  
Neil B. Madsen
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Dietary Factors ◽  
Sodium Oxalate ◽  
Rat Serum ◽  
Growing Rats ◽  
Calcium And Magnesium ◽  
Relationship Of ◽  
The Relationship ◽  
Normal Calcium

Increased levels of serum alkaline phosphatase were produced in growing rats by diets low in calcium or by the addition of sodium oxalate or rhubarb to diets containing adequate amounts of calcium. In addition, variations from normal in levels of serum phosphorus, calcium, and magnesium, as well as the ash of the tibiae, indicate that the animals were rachitic. The amount of calcium retained in the bodies of animals maintained on a diet containing rhubarb, which has a high oxalate content, were very much lower than in growing rats fed a normal calcium diet.

THE RELATIONSHIP OF DIETARY FACTORS TO RAT SERUM ALKALINE PHOSPHATASE

1952 ◽  
Vol 30 (5) ◽  
pp. 378-382
Author(s):  
Jules Tuba ◽  
Bohdan Jelinek ◽  
Ridley K. Shaw ◽  
Neil B. Madsen
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Wheat Gluten ◽  
Dietary Factors ◽  
Male Rats ◽  
Brewer’S Yeast ◽  
Daily Consumption ◽  
Brewer's Yeast

Weanling rats were fed dietary concentrations of 0%, 5%, 10%, 30%, and 91% casein. Complete absence of dietary protein resulted in death of the young animals within five weeks. Activity of serum alkaline phosphatase was at starvation levels during this period. Optimal growth was obtained at 30% dietary level of casein, and alkaline phosphatase activity was in the normal range. No effect on the enzyme could be attributed to the protein. There is a highly significant correlation between daily food consumption and serum phosphatase activity, which is attributed to the fat component of the various diets. Adult male rats were subjected to a three-week period of protein depletion, which was followed by a two-week period of protein repletion. The protein was supplied in the following forms: casein, dried brewer’s yeast, wheat gluten, casein plus wheat gluten, and dried brewer’s yeast plus gluten. Enzyme values at the end of the experiment were typical for the high fat diet (26%) which these animals were receiving. Once more there is a significant correlation between serum alkaline phosphatase activity and daily consumption of fat, whereas the protein component of the diet is without effect on the enzyme.

Alkaline phosphatase activity in the plasma of children and adolescents.

1977 ◽  
Vol 23 (3) ◽  
pp. 469-472 ◽  
Author(s):  
G A Fleisher ◽  
E S Eickelberg ◽  
L R Elveback
Keyword(s):  
Alkaline Phosphatase ◽  
Public Schools ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Intraclass Correlation ◽  
Serum Alkaline Phosphatase Activity ◽  
Upper Limits ◽  
Prepubertal Girls

Abstract We determined plasma (serum alkaline phosphatase activity in 854 healthy students of the Rochester, Minnesota, public schools. Prepubertal girls had somewhat greater upper limits than did boys, and there was a low trend of increasing activity in both sexes. At the beginning of adolescence increasing activities were observed, which peaked at ages 11 to 12 years in girls and at ages 13 to 14 in boys. Adult values were not reached until six to eight years later. In 180 pairs of siblings, a significant intraclass correlation was noted. A possible role of alkaline phosphatase in the regulation of protein synthesis is suggested.

Effects of various fibrates on serum alkaline phosphatase activity

2002 ◽  
Vol 165 (1) ◽  
pp. 187-188 ◽  
Author(s):  
Emanuel Ganotakis ◽  
Vasilios Tsimihodimos ◽  
Eleni Bairaktari ◽  
Evagelos Rizos ◽  
Vasilios Athyros ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase Activity
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