THE LACTIC DEHYDROGENASE ACTIVITY AND PYRIDINE NUCLEOTIDE CONTENT OF HUMAN BLOOD CELLS IN ANEMIA

1951 ◽  
Vol 29 (3) ◽  
pp. 108-113
Author(s):  
M. C. Blanchaer ◽  
Pamela Weiss ◽  
D. E. Bergsagel
Keyword(s):  
Dehydrogenase Activity ◽  
Blood Cells ◽  
Hemoglobin Concentration ◽  
Normal Subjects ◽  
Lactic Dehydrogenase ◽  
Pyridine Nucleotide ◽  
Nucleotide Content ◽  
Human Blood Cells ◽  
Potential Activity ◽  
The Relationship

The lactic dehydrogenase activity and the pyridine nucleotide content of the blood cells (PN/BC) of two normal subjects and 12 patients with various degrees of anemia was measured to determine whether the previously reported elevated PN/BC values in anemia are related to an altered metabolic activity of the cells. In 12 well nourished subjects it was found that the PN/BC, expressed in μgm. DPN per gm. of hemoglobin, was elevated in proportion to the severity of the anemia and that this increase was accompanied by a proportional rise in the cellular lactic dehydrogenase activity. The low PN/BC of two poorly nourished patients was accompanied by correspondingly low lactic dehydrogenase levels. The correlation coefficient for the relationship between the lactic dehydrogenase and PN/BC values for all the subjects was + 0.89 and was considered significant since P < 0.01. The results suggest that the potential activity of an important enzymatic step in erythrocyte glycolysis is increased in anemia in proportion to the decrease in the hemoglobin concentration and the fall in the hematocrit. The increased PN/BC under these conditions is probably a reflection of an elevated cellular diphosphopyridine nucleotide concentration.

THE PYRIDINE NUCLEOTIDE CONTENT OF HUMAN BLOOD CELLS IN ANEMIA

1950 ◽  
Vol 28e (1) ◽  
pp. 12-18
Author(s):  
M. C. Blanchaer
Keyword(s):  
Blood Cells ◽  
Hemoglobin Concentration ◽  
Normal Subjects ◽  
Pyridine Nucleotide ◽  
Adequate Diet ◽  
Nucleotide Content ◽  
Human Blood Cells ◽  
Normal Individuals ◽  
Hospital Patients ◽  
Red Cell Count

The pyridine nucleotide content of the blood cells was measured in five normal individuals and in 27 hospital patients with various degrees of anemia. The pyridine nucleotide values of 12 anemic patients who had consumed an adequate diet for some time prior to the study were higher than those of the normal subjects. A negative correlation was observed between the cellular pyridine nucleotide content and the severity of the anemia when the latter was expressed as the logarithm of either the red cell count or the hemoglobin concentration. The remaining 15 patients had dietary histories suggestive of a low intake of niacin and protein. The blood cell pyridine nucleotide levels of this group were of the same order as those of the normal subjects but in most cases were distinctly below those of the well nourished anemic patients with a comparable degree of anemia.

THE EFFECT OF NIACIN ON THE PYRIDINE NUCLEOTIDE CONTENT OF HUMAN BLOOD CELLS IN ANEMIA

1950 ◽  
Vol 28e (3) ◽  
pp. 97-105
Author(s):  
M. C. Blanchaer ◽  
D. E. Bergsagel ◽  
Pamela Weiss ◽  
Dorothy E. Jefferson
Keyword(s):  
Nicotinic Acid ◽  
Blood Cells ◽  
Normal Subjects ◽  
Pyridine Nucleotide ◽  
Nucleotide Content ◽  
Human Blood Cells ◽  
Normal Individuals ◽  
Before And After ◽  
Niacin Therapy ◽  
Normal Controls

The pyridine nucleotide content of the blood cells (PN/BG) of four normal individuals and five anemic patients was measured repeatedly before and after supplementing the diet with niacin. Daily doses of 50 mgm. nicotinic acid or nicotinamide failed to affect the values in the normal subjects and two well-nourished anemic patients. The same dose of nicotinic acid rapidly raised the PN/BC of three poorly nourished anemic patients to values approximating those of well-nourished subjects with similar degrees of anemia. The results obtained after saturation with niacin confirmed the previous report that the PN/BC is higher in anemic patients than in normal controls and that a negative correlation exists between the pyridine nucleotide values and the severity of the anemia. In spite of continued niacin therapy, correction of the anemia was accompanied by a gradual decrease in the values until they approached those of the normal subjects. The present findings also confirm the earlier report that changes in the PN/BC are apparently independent of moderate variations in the number of circulating leucocytes and reticulocytes and bear no relationship to the size or hemoglobin content of the red cells. The significance of these findings in relation to human niacin nutrition is discussed.

Dehydrogenase of Regenerating Red Blood Cells in Phenylhydrazine Anemia

1962 ◽  
Vol 8 (5) ◽  
pp. 486-496 ◽  
Author(s):  
Max M Friedman ◽  
Bernard S Kahn ◽  
Bernard Lapan
Keyword(s):  
Red Blood Cells ◽  
Dehydrogenase Activity ◽  
Blood Cells ◽  
Lactic Dehydrogenase ◽  
Red Cell ◽  
Average Population

Abstract Dehydrogenase activities in red blood cells of rabbits made anemic with phenylhydrazine were assayed. These assays were continued at the time of reticulocytosis and followed through the course of in vivo maturation of the reticulocytes to a younger erythrocyte population. Isocitric, glucose-6-phosphate, and 6-phosphogluconic dehydrogenases were more active in the reticulocytes. Isocitric and glucose-6-phosphate dehydrogenases were also more active in the younger red cell, compared to an average population. Lactic dehydrogenase activity was the same in the reticulocyte, young red cell, and the older red cell. The significance of these findings is discussed.

Nonlinear Analysis of Visual Evoked Potentials Elicited by Color Stimulation

1997 ◽  
Vol 36 (04/05) ◽  
pp. 315-318 ◽  
Author(s):  
K. Momose ◽  
K. Komiya ◽  
A. Uchiyama
Keyword(s):  
Visual System ◽  
Evoked Potentials ◽  
Visual Evoked Potentials ◽  
Normal Subjects ◽  
Second Order ◽  
First Order ◽  
The Relationship ◽  
Perceived Color ◽  
Second Order Nonlinearity ◽  
Visual Evoked

Abstract:The relationship between chromatically modulated stimuli and visual evoked potentials (VEPs) was considered. VEPs of normal subjects elicited by chromatically modulated stimuli were measured under several color adaptations, and their binary kernels were estimated. Up to the second-order, binary kernels obtained from VEPs were so characteristic that the VEP-chromatic modulation system showed second-order nonlinearity. First-order binary kernels depended on the color of the stimulus and adaptation, whereas second-order kernels showed almost no difference. This result indicates that the waveforms of first-order binary kernels reflect perceived color (hue). This supports the suggestion that kernels of VEPs include color responses, and could be used as a probe with which to examine the color visual system.

The Relationship of Root Mean Square Value of Electromyography and Isometric Torque of Quadriceps in Normal Subjects

2016 ◽  
Vol 26 (1) ◽  
pp. 58
Author(s):  
Qiurong XIE ◽  
Zheng JIANG ◽  
Qinglu LUO ◽  
Jie LIANG ◽  
Xiaoling WANG ◽  
...  
Keyword(s):  
Root Mean Square ◽  
Normal Subjects ◽  
Mean Square ◽  
Isometric Torque ◽  
Relationship Of ◽  
The Relationship
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