scholarly journals Hyaluronan content and distribution in the rat ventral prostate after castration

2018 ◽  
Vol 96 (5) ◽  
pp. 556-563
Author(s):  
Heloisa H.M. Della-Colleta ◽  
Hernandes F. Carvalho
Keyword(s):  
Epithelial Cells ◽  
Tumor Growth ◽  
Reverse Transcription ◽  
Stromal Cells ◽  
Total Content ◽  
Ventral Prostate ◽  
Rt Pcr ◽  
Qrt Pcr ◽  
Hyaluronan Synthases ◽  
Rat Ventral Prostate

Hyaluronan (HA) has been implicated in tissue remodeling, healing, and tumor growth. This study investigated the variation in hyaluronan content, distribution, and metabolism in the rat ventral prostate (VP) in response to androgen deprivation after castration. The mRNA abundance of hyaluronan synthases (Has1–3) and hyaluronidases (Hyal 1–3) were assessed by reverse transcription (RT)–PCR and immunohistochemistry, respectively. The results demonstrated an increased concentration, but an overall reduction in HA content. HA was located in both epithelium and stroma of the prostate of both the noncastrated and castrated animals. Quantitative RT–PCR (qRT–PCR) showed that Has1 and Has2 are major synthases, and that Hyal 1 was the predominant hydrolase expressed in the VP. qRT–PCR also showed that Has1 and Has2 mRNA increased transiently after castration, whereas Has3 mRNA declined markedly. While Hyal 1 mRNA increased slowly up to day 21 after castration, Hyal 2 and Hyal 3 mRNA dropped significantly. CD44 was found in the epithelial cells and in some stromal cells in both hormonal conditions. In conclusion, castration results in increased abundance of Has1 and Has2 mRNA, but is associated with a decrease in the total content of HA, with an increased concentration, and a predominance of short-chain HA molecules.

Androgen metabolism and actions in rat ventral prostate epithelial and stromal cell cultures

1986 ◽  
Vol 64 (6) ◽  
pp. 583-593 ◽  
Author(s):  
J. Orlowski ◽  
A. F. Clark
Keyword(s):  
Epithelial Cells ◽  
Cell Cultures ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Cultured Cells ◽  
Cell Types ◽  
Ventral Prostate ◽  
Oxidoreductase Activity ◽  
Androgen Metabolism ◽  
Rat Ventral Prostate

The rat ventral prostate requires androgens for normal development, growth, and function. To investigate the relationship between androgen metabolism and its effects in the prostate and to examine differences between the epithelial and stromal cells, we have established a system of primary cell cultures of immature rat ventral prostate cells. Cultures of both cell types after reaching confluency (6–7 days) actively metabolized 3H-labelled testosterone (T), 5α-dihydrotestosterone (5α-DHT), 5α-androstane-3α,17β-diol, and 5α-androstane-3β,17β-diol. The epithelial cells actively reduced T to 5α-DHT and formed significant amounts of 5α-androstane-3,17-dione from T, 5α-DHT, and 5α-androstane-3α,17β-diol. All substrates were converted to significant amounts of C19O3metabolites. The stromal cells also metabolized all substrates, but very little 5α-androstane-3,17-dione was formed. The metabolism studies indicate that both cell types have Δ4-5α-reductase, 3α- and 3β-hydroxysteroid oxidoreductase and hydroxylase activities. The epithelial cells have significant 17β-hydroxysteroid oxidoreductase activity. The epithelial cells cultures grown in the presence of T have higher acid phosphatase (AP) contents (demonstrated histochemically and by biochemical assay). Tartrate inhibition studies indicate that the epithelial cells grown in the presence of T are making secretory AP. Stromal cell AP is not influenced by T. The results indicate that the cultured cells maintain differentiated prostatic functions: ability to metabolize androgens and, in the case of the epithelial cells, synthesize secretory AP.

Ca2+-activated Cl? channel currents in rat ventral prostate epithelial cells

The Prostate ◽  
2003 ◽  
Vol 55 (2) ◽  
pp. 118-127 ◽  
Author(s):  
Sung Joon Kim ◽  
Sun Young Shin ◽  
Ji Eun Lee ◽  
Jun Hee Kim ◽  
Dae-Yong Uhm
Keyword(s):  
Epithelial Cells ◽  
Ventral Prostate ◽  
Cl Channel ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate ◽  
Channel Currents

Differences in growth requirements between epithelial and stromal cells derived from rat ventral prostate in serum-free primary culture

The Prostate ◽  
1990 ◽  
Vol 17 (3) ◽  
pp. 207-218 ◽  
Author(s):  
Shigeo Taketa ◽  
Nozomu Nishi ◽  
Hirotoshi Takasuga ◽  
Takuya Okutani ◽  
Ikumasa Takenaka ◽  
...  
Keyword(s):  
Primary Culture ◽  
Stromal Cells ◽  
Ventral Prostate ◽  
Serum Free ◽  
Growth Requirements ◽  
Rat Ventral Prostate

K+ channel currents in rat ventral prostate epithelial cells

The Prostate ◽  
2002 ◽  
Vol 51 (3) ◽  
pp. 201-210 ◽  
Author(s):  
Jun Hee Kim ◽  
Eun-Kyung Hong ◽  
Hee Sook Choi ◽  
Seung-Joon Oh ◽  
Kwang Myung Kim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ventral Prostate ◽  
K Channel ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate ◽  
Channel Currents

scholarly journals Regulation of 5α-Reductase Isoforms by Oxytocin in the Rat Ventral Prostate

Endocrinology ◽  
2004 ◽  
Vol 145 (12) ◽  
pp. 5767-5773 ◽  
Author(s):  
S. J. Assinder ◽  
C. Johnson ◽  
K. King ◽  
H. D. Nicholson
Keyword(s):  
Reproductive Tract ◽  
Differential Regulation ◽  
Biologically Active ◽  
Ventral Prostate ◽  
Saline Control ◽  
Rt Pcr ◽  
Male Reproductive Tract ◽  
Rat Ventral Prostate ◽  
Stromal Tissue ◽  
Male Wistar Rats

Abstract Oxytocin (OT) is present in the male reproductive tract, where it is known to modulate contractility, cell growth, and steroidogenesis. Little is known about how OT regulates these processes. This study describes the localization of OT receptor in the rat ventral prostate and investigates if OT regulates gene expression and/or activity of 5α-reductase isoforms I and II. The ventral prostates of adult male Wistar rats were collected following daily sc administration of saline (control), OT, a specific OT antagonist or both OT plus antagonist for 3 d. Expression of the OT receptor was identified in the ventral prostate by RT-PCR and Western blot, and confirmed to be a single active binding site by radioreceptor assay. Immunohistochemistry localized the receptor to the epithelium of prostatic acini and to the stromal tissue. Real-time RT-PCR determined that OT treatment significantly reduced expression of 5α-reductase I but significantly increased 5α-reductase II expression in the ventral prostate. Activity of both isoforms of 5α-reductase was significantly increased by OT, resulting in increased concentration of prostatic dihydrotestosterone. In conclusion, OT is involved in regulating conversion of testosterone to the biologically active dihydrotestosterone in the rat ventral prostate. It does so by differential regulation of 5α-reductase isoforms I and II.

scholarly journals Androgens Regulate the Immune/Inflammatory Response and Cell Survival Pathways in Rat Ventral Prostate Epithelial Cells

Endocrinology ◽  
2006 ◽  
Vol 147 (1) ◽  
pp. 257-271 ◽  
Author(s):  
A. J. Asirvatham ◽  
M. Schmidt ◽  
B. Gao ◽  
J. Chaudhary
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cells ◽  
Inflammatory Response ◽  
Immediate Early Genes ◽  
Immediate Early ◽  
Ventral Prostate ◽  
Cell Renewal ◽  
Early Genes ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate

A major hurdle in understanding the role of androgens is the heterogeneity of androgen receptor (AR) expression in the prostate. Because the majority of prostate cancer arises from the AR-positive secretory luminal epithelial cells, identifying the androgen-mediated pathways in the prostate epithelium is of great significance to understanding their role in prostate pathogenesis. To meet this objective, the current study was designed to identify immediate-early genes expressed in response to the synthetic androgen R1881 in cultured rat ventral prostate epithelial cells. Rat ventral prostate epithelial cells, purified from 20-d-old rats, were cultured, and the presence of AR and the response to androgen were established. The cells were then treated with R1881 for 2 and 12 h to capture immediate-early genes in an Affymetrix-based gene chip platform. A total of 66 nonredundant genes were identified that were responsive to R1881. The functional androgen response elements were identified in the proximal promoter to determine possible molecular mechanism. Cluster analysis identified five distinct signatures of R1881-induced genes. Pathway analysis suggested that R1881 primarily influences cell proliferation/differentiation and inflammatory/immune response pathways. Androgens appear to regulate cell renewal by regulating differentiation, cell proliferation, and apoptosis. Two mutually exclusive inflammatory response pathways were observed. The interferon pathway was up-regulated, and the ILs were down-regulated. The data identified novel androgen-regulated genes (e.g. Id1, Id3, IL-6, IGF-binding protein-2 and -3, and JunB). The loss of androgen regulation of these genes can have important consequences for cellular transformation and transition to androgen-independent growth and survival.

Isolation, culture and characterization of epithelial cells derived from rat ventral prostate

1980 ◽  
Vol 197 (2) ◽  
pp. 239-256 ◽  
Author(s):  
William H. J. Douglas ◽  
Louis Terracio ◽  
Howard Glass
Keyword(s):  
Epithelial Cells ◽  
Ventral Prostate ◽  
Rat Ventral Prostate
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