scholarly journals Regulation of 5α-Reductase Isoforms by Oxytocin in the Rat Ventral Prostate

Endocrinology ◽  
2004 ◽  
Vol 145 (12) ◽  
pp. 5767-5773 ◽  
Author(s):  
S. J. Assinder ◽  
C. Johnson ◽  
K. King ◽  
H. D. Nicholson
Keyword(s):  
Reproductive Tract ◽  
Differential Regulation ◽  
Biologically Active ◽  
Ventral Prostate ◽  
Saline Control ◽  
Rt Pcr ◽  
Male Reproductive Tract ◽  
Rat Ventral Prostate ◽  
Stromal Tissue ◽  
Male Wistar Rats

Abstract Oxytocin (OT) is present in the male reproductive tract, where it is known to modulate contractility, cell growth, and steroidogenesis. Little is known about how OT regulates these processes. This study describes the localization of OT receptor in the rat ventral prostate and investigates if OT regulates gene expression and/or activity of 5α-reductase isoforms I and II. The ventral prostates of adult male Wistar rats were collected following daily sc administration of saline (control), OT, a specific OT antagonist or both OT plus antagonist for 3 d. Expression of the OT receptor was identified in the ventral prostate by RT-PCR and Western blot, and confirmed to be a single active binding site by radioreceptor assay. Immunohistochemistry localized the receptor to the epithelium of prostatic acini and to the stromal tissue. Real-time RT-PCR determined that OT treatment significantly reduced expression of 5α-reductase I but significantly increased 5α-reductase II expression in the ventral prostate. Activity of both isoforms of 5α-reductase was significantly increased by OT, resulting in increased concentration of prostatic dihydrotestosterone. In conclusion, OT is involved in regulating conversion of testosterone to the biologically active dihydrotestosterone in the rat ventral prostate. It does so by differential regulation of 5α-reductase isoforms I and II.

Phthalate affect the reproductive function and sexual behavior of male Wistar rats

2006 ◽  
Vol 25 (6) ◽  
pp. 297-303 ◽  
Author(s):  
P R Dalsenter ◽  
G M Santana ◽  
S W Grande ◽  
A Jm Andrade ◽  
S L Araújo
Keyword(s):  
Sexual Behavior ◽  
Adverse Effects ◽  
Reproductive Tract ◽  
Reproductive Function ◽  
In Utero ◽  
Ventral Prostate ◽  
Sperm Counts ◽  
Male Wistar Rats ◽  
Plastic Toys ◽  
Ethylhexyl Phthalate

Phthalates are chemicals used in many industrial products (plastic toys, shampoos, soaps), and are suspected of inducing adverse effects on the male reproductive system. In the present study, we evaluated the effects of the plasticizer di-(2-ethylhexyl)-phthalate (DEHP) on the reproductive function and sexual behavior of male offspring rats, exposed in utero and during lactation (0, 20, 100 and 500 mg/kg per day by gavage). The effects produced clearly demonstrate the ability of DEHP to disrupt the androgen-regulated development of the male reproductive tract. Absolute and relative weights of androgen-dependent tissue organs (ventral prostate and seminal vesicle) were significantly reduced at the highest dose level tested (500 mg/kg per day). Impairment of male sexual behavior (500 mg/kg per day) was also observed. Moreover, the reduction in daily sperm production and epididymal sperm counts observed after administration of the highest dose suggests an impairment of the spermatogenic processes. Most of the adverse effects reported here were observed both during puberty and during adulthood, indicating permanent effects of in utero and lactational DEHP exposure.

scholarly journals Hyaluronan content and distribution in the rat ventral prostate after castration

2018 ◽  
Vol 96 (5) ◽  
pp. 556-563
Author(s):  
Heloisa H.M. Della-Colleta ◽  
Hernandes F. Carvalho
Keyword(s):  
Epithelial Cells ◽  
Tumor Growth ◽  
Reverse Transcription ◽  
Stromal Cells ◽  
Total Content ◽  
Ventral Prostate ◽  
Rt Pcr ◽  
Qrt Pcr ◽  
Hyaluronan Synthases ◽  
Rat Ventral Prostate

Hyaluronan (HA) has been implicated in tissue remodeling, healing, and tumor growth. This study investigated the variation in hyaluronan content, distribution, and metabolism in the rat ventral prostate (VP) in response to androgen deprivation after castration. The mRNA abundance of hyaluronan synthases (Has1–3) and hyaluronidases (Hyal 1–3) were assessed by reverse transcription (RT)–PCR and immunohistochemistry, respectively. The results demonstrated an increased concentration, but an overall reduction in HA content. HA was located in both epithelium and stroma of the prostate of both the noncastrated and castrated animals. Quantitative RT–PCR (qRT–PCR) showed that Has1 and Has2 are major synthases, and that Hyal 1 was the predominant hydrolase expressed in the VP. qRT–PCR also showed that Has1 and Has2 mRNA increased transiently after castration, whereas Has3 mRNA declined markedly. While Hyal 1 mRNA increased slowly up to day 21 after castration, Hyal 2 and Hyal 3 mRNA dropped significantly. CD44 was found in the epithelial cells and in some stromal cells in both hormonal conditions. In conclusion, castration results in increased abundance of Has1 and Has2 mRNA, but is associated with a decrease in the total content of HA, with an increased concentration, and a predominance of short-chain HA molecules.

scholarly journals Differential regulation of protooncogene c-myc expression in rat ventral prostate after castration

IUBMB Life ◽  
1999 ◽  
Vol 47 (1) ◽  
pp. 143-151
Author(s):  
Joong-Hwa Lee ◽  
Chong-Koo Sul ◽  
Young-Kyun Kim ◽  
Byung-Doo Hwang ◽  
Kyu Lim
Keyword(s):  
Differential Regulation ◽  
Ventral Prostate ◽  
Rat Ventral Prostate

The Effect of PDE5 Inhibitors on the Male Reproductive Tract

2020 ◽  
Vol 26 ◽  
Author(s):  
Nikolaos Sofikitis ◽  
Aris Kaltsas ◽  
Fotios Dimitriadis ◽  
Jens Rassweiler ◽  
Nikolaos Grivas ◽  
...  
Keyword(s):  
Male Infertility ◽  
Reproductive Tract ◽  
Tunica Albuginea ◽  
Scientific Data ◽  
Secretory Function ◽  
Pde5 Inhibitors ◽  
Male Reproductive Tract ◽  
Review Study ◽  
Phosphodiesterase 5

The therapeutic range of cyclic nucleotide phosphodiesterase 5 inhibitors (PDE5) inhibitors is getting wider in the last years. This review study focuses on the potential employment of PDE5 inhibitors as an adjunct tool for the therapeutic management of male infertility. The literature tends to suggest a beneficial effect of PDE5 inhibitors on Leydig and Sertoli cells secretory function. It also appears that PDE5 inhibitors play a role in the regulation of the contractility of the testicular tunica albuginea and the epididymis. Moreover scientific data suggest that PDE5 inhibitors enhance the prostatic secretory function leading to an improvement in sperm motility. Other studies additionally demonstrate a role of PDE5 inhibitors in the regulation of sperm capacitation process. Placebo-controlled, randomized, blind studies are necessary to unambiguously incorporate PDE5 inhibitors as an adjunct tool for the pharmaceutical treatment of semen disorders and male infertility.

Changes in the Male Reproductive Organs of the Dugong, Dugong dugon (Sirenia: Dugondidae) with Age and Reproductive Activity

1984 ◽  
Vol 32 (6) ◽  
pp. 721 ◽  
Author(s):  
H Marsh ◽  
GE Heinsohn ◽  
TD Glover
Keyword(s):  
Reproductive Tract ◽  
Prostate Gland ◽  
Reproductive Activity ◽  
Reproductive Organs ◽  
Seminal Vesicles ◽  
Growth Layer ◽  
Male Reproductive Tract ◽  
Qualitative And Quantitative ◽  
Quantitative Examination ◽  
Testicular Histology

The anatomy and histology of the male reproductive tract of the dugong (Dugong dugon) is described. Each testis and its adjacent epididymis lie immediately caudal to the corresponding kidney. The seminal vesicles are large but there is no discrete prostate gland and the bulbo-urethral glands are also diffuse. Both qualitative and quantitative examination of the testes and epididymides of 59 males whose ages have been estimated from tusk dentinal growth layer counts indicate that the male dugong does not produce spermatozoa continuously, despite the absence of a distinct breeding season. Individual dugongs were observed with testes at all stages between complete quiescence and full spermatogenesis, and only 10 of the 40 mature males had fully spermatogenic testes and epididymides packed with spermatozoa. Androgenic and spermatogenic activity of the testes appeared to be in phase, but the testicular histology of some old males suggested that they may have been sterile for long periods.

Methyl-parathion bioactivation in the male reproductive tract: From mRNA to protein expression of involved CYP isoforms

2009 ◽  
Vol 189 ◽  
pp. S145
Author(s):  
Betzabet Quintanilla-Vega ◽  
Patricia Espíritu-Gordillo ◽  
Yuliana Palacios-Gil ◽  
Margarita Guaderrama-Díaz ◽  
María de Jesús Solís-Heredia ◽  
...  
Keyword(s):  
Protein Expression ◽  
Methyl Parathion ◽  
Reproductive Tract ◽  
Male Reproductive Tract ◽  
Cyp Isoforms
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