Ca2+-activated Cl? channel currents in rat ventral prostate epithelial cells

The Prostate ◽  
2003 ◽  
Vol 55 (2) ◽  
pp. 118-127 ◽  
Author(s):  
Sung Joon Kim ◽  
Sun Young Shin ◽  
Ji Eun Lee ◽  
Jun Hee Kim ◽  
Dae-Yong Uhm
Keyword(s):  
Epithelial Cells ◽  
Ventral Prostate ◽  
Cl Channel ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate ◽  
Channel Currents

K+ channel currents in rat ventral prostate epithelial cells

The Prostate ◽  
2002 ◽  
Vol 51 (3) ◽  
pp. 201-210 ◽  
Author(s):  
Jun Hee Kim ◽  
Eun-Kyung Hong ◽  
Hee Sook Choi ◽  
Seung-Joon Oh ◽  
Kwang Myung Kim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ventral Prostate ◽  
K Channel ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate ◽  
Channel Currents

scholarly journals Androgens Regulate the Immune/Inflammatory Response and Cell Survival Pathways in Rat Ventral Prostate Epithelial Cells

Endocrinology ◽  
2006 ◽  
Vol 147 (1) ◽  
pp. 257-271 ◽  
Author(s):  
A. J. Asirvatham ◽  
M. Schmidt ◽  
B. Gao ◽  
J. Chaudhary
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cells ◽  
Inflammatory Response ◽  
Immediate Early Genes ◽  
Immediate Early ◽  
Ventral Prostate ◽  
Cell Renewal ◽  
Early Genes ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate

A major hurdle in understanding the role of androgens is the heterogeneity of androgen receptor (AR) expression in the prostate. Because the majority of prostate cancer arises from the AR-positive secretory luminal epithelial cells, identifying the androgen-mediated pathways in the prostate epithelium is of great significance to understanding their role in prostate pathogenesis. To meet this objective, the current study was designed to identify immediate-early genes expressed in response to the synthetic androgen R1881 in cultured rat ventral prostate epithelial cells. Rat ventral prostate epithelial cells, purified from 20-d-old rats, were cultured, and the presence of AR and the response to androgen were established. The cells were then treated with R1881 for 2 and 12 h to capture immediate-early genes in an Affymetrix-based gene chip platform. A total of 66 nonredundant genes were identified that were responsive to R1881. The functional androgen response elements were identified in the proximal promoter to determine possible molecular mechanism. Cluster analysis identified five distinct signatures of R1881-induced genes. Pathway analysis suggested that R1881 primarily influences cell proliferation/differentiation and inflammatory/immune response pathways. Androgens appear to regulate cell renewal by regulating differentiation, cell proliferation, and apoptosis. Two mutually exclusive inflammatory response pathways were observed. The interferon pathway was up-regulated, and the ILs were down-regulated. The data identified novel androgen-regulated genes (e.g. Id1, Id3, IL-6, IGF-binding protein-2 and -3, and JunB). The loss of androgen regulation of these genes can have important consequences for cellular transformation and transition to androgen-independent growth and survival.

Rat ventral prostate xanthine oxidase–mediated metabolism of acetaldehyde to acetyl radical

2009 ◽  
Vol 28 (4) ◽  
pp. 203-208 ◽  
Author(s):  
GD Castro ◽  
MH Costantini ◽  
JA Castro
Keyword(s):  
Epithelial Cells ◽  
Xanthine Oxidase ◽  
Alcohol Drinking ◽  
Metabolic Pathways ◽  
Ventral Prostate ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate ◽  
Low Levels ◽  
Acetyl Radical ◽  
And Oxidative Stress

Alcohol drinking is known to lead to deleterious effects on prostate epithelial cells from humans and experimental animals. The understanding of the mechanisms underlying these effects is relevant to intraprostatic ethanol treatment of benign prostatic hyperplasia and to shed some light into the conflictive results linking alcohol consumption to prostate cancer. In previous studies, we provided evidence about the presence in the rat ventral prostate of cytosolic and microsomal metabolic pathways of ethanol to acetaldehyde and 1-hydroxyethyl radical and about the low levels of alcohol dehydrogenase and aldehyde dehydrogenase. Acetaldehyde accumulation in prostate tissue and oxidative stress promotion were also observed. In this study, we report that in the ventral prostate cytosolic fraction, xanthine oxidoreductase is able to metabolize acetaldehyde to acetyl radical. The identification of the acetyl was performed by GC-MS of the silylated acetyl-PBN adduct. Reference adduct was generated chemically. Formation of acetyl was also observed using pure xanthine oxidase. The generation of acetyl by the prostate cytosol was inhibited by allopurinol, oxypurinol, diphenyleneiodonium chloride, folate, and ellagic acid. Results suggest that metabolism of ethanol to acetaldehyde and to 1-hydroxyethyl and acetyl radicals could be involved in the deleterious effects of alcohol drinking on prostate epithelial cells.

Secretion of inhibin-like material by rat ventral prostate epithelial cells in culture

The Prostate ◽  
1991 ◽  
Vol 18 (1) ◽  
pp. 59-71 ◽  
Author(s):  
Kusum Kharbanda ◽  
Sushma R. Dhingra ◽  
S. Duraiswami
Keyword(s):  
Epithelial Cells ◽  
Ventral Prostate ◽  
Cells In Culture ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate
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