Growth of micropropagated lowbush blueberry with defined fungi in irradiated peat mix

1992 ◽  
Vol 70 (11) ◽  
pp. 2202-2206 ◽  
Author(s):  
Walter Litten ◽  
John M. Smagula ◽  
Yolande Dalpé
Keyword(s):  
Mycorrhizal Fungi ◽  
Stem Tissue ◽  
Γ Irradiation ◽  
Vaccinium Angustifolium ◽  
Hymenoscyphus Ericae ◽  
Ericoid Mycorrhizae ◽  
Lowbush Blueberry ◽  
Vegetative Multiplication ◽  
Mycorrhizal Inoculation

There is an interest in vegetative multiplication of high-yielding clones of Vaccinium angustifolium Ait. to establish or enhance blueberry production. This study evaluates mycorrhizal inoculation as an aid in such propagation from microcuttings. Shoots of Vaccinium angustifolium (clone 7062) generated in vitro were rooted in a peat–vermiculite–perlite substrate with or without ericoid mycorrhizal fungi fortification by Hymenoscyphus ericae or Scytalidium vaccinii and with or without peat sterilization by γ irradiation. Both in irradiated peat mix inoculated with S. vaccinii and in unirradiated peat mix with H. ericae, microcuttings grew taller and branched more than with the four other treatments. The profusely rooted plantlets available from all treatments of the cuttings put on significantly more total length of stems and branches after 167 days in the greenhouse when growing with either inoculant in unirradiated peat than in the unirradiated peat without inoculation. However, the magnitude of difference might be of borderline importance in commercial nursery operations. A higher level of copper and zinc in stem tissue was observed in stem tissue of plants grown with H. ericae with or without irradiation but not with S. vaccinii. Key words: ericoid mycorrhizae, micropropagation, Hymenoscyphus ericae, Scytalidium vaccinii, Vaccinium angustifolium.

scholarly journals In Vitro Colonization of Micropropagated Pieris floribunda by Ericoid Mycorrhizae. II. Effects on Acclimatization and Growth

HortScience ◽  
2001 ◽  
Vol 36 (2) ◽  
pp. 357-359 ◽  
Author(s):  
Mark C. Starrett ◽  
Frank A. Blazich ◽  
Steven R. Shafer ◽  
Larry F. Grand
Keyword(s):  
Mycorrhizal Fungi ◽  
Hymenoscyphus Ericae ◽  
Ericoid Mycorrhizal Fungi ◽  
Ericoid Mycorrhizae ◽  
In Vitro Inoculation

Inoculation of microshoots of Pieris floribunda (Pursh ex Sims) Benth. and Hook. (mountain andromeda) with isolates of Hymenoscyphus ericae (Read) Korf and Kernan ericoid mycorrhizal fungi stimulated growth during 1 month in vitro. However, no benefits were apparent after 3 months in a greenhouse. Acclimatization of plantlets of P. floribunda to greenhouse conditions following in vitro inoculation improved survival (42% vs. 16% for controls). The protocol reported herein is similar to procedures utilized currently for micropropagation of various ericaceous species and has potential to improve plantlet survival during acclimatization.

Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro

2018 ◽  
Vol 32 (4) ◽  
pp. 705-714 ◽  
Author(s):  
Christina Ly ◽  
Jonathan Ferrier ◽  
Jeremiah Gaudet ◽  
Julien Yockell-Lelièvre ◽  
John Thor Arnason ◽  
...  
Keyword(s):  
Cell Migration ◽  
Leaf Extract ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Migration And Invasion ◽  
Vaccinium Angustifolium ◽  
Cell Migration And Invasion ◽  
Lowbush Blueberry

DNA methylation in lowbush blueberry (Vaccinium angustifolium Ait.) propagated by softwood cutting and tissue culture

2018 ◽  
Vol 98 (5) ◽  
pp. 1035-1044 ◽  
Author(s):  
Juran C. Goyali ◽  
Abir U. Igamberdiev ◽  
Samir C. Debnath
Keyword(s):  
Dna Methylation ◽  
Tissue Culture ◽  
Growth Conditions ◽  
Vaccinium Angustifolium ◽  
Lowbush Blueberry ◽  
Restriction Sites ◽  
Methylation Sensitive Amplification Polymorphism ◽  
Softwood Cutting

Plant DNA methylation is one of the frequent epigenetic variations induced by tissue culture. Global DNA methylation was evaluated in lowbush blueberry (Vaccinium angustifolium Ait.) wild clone QB9C and cultivar Fundy propagated by conventional softwood cutting (SC) and tissue culture (TC) using the methylation-sensitive amplification polymorphism (MSAP) technique. In all, 106 and 107 DNA fragments were amplified using 16 selective primer combinations in SC plants of QB9C and Fundy, respectively. In micropropagated QB9C and Fundy plants, there were 105 and 109 amplified fragments, respectively. Overall, 25% of restriction sites were methylated at the cytosine nucleotide in QB9C plants propagated by SC compared with 19% in Fundy. In contrast, a total of 29% and 20% of restriction sites were methylated at cytosine in micropropagated QB9C and Fundy plants, respectively. Tissue culture plants demonstrated higher methylation events than SC plants in both genotypes. Previously, methylation polymorphism has been detected in TC plants but not in SC counterparts. Different patterns of DNA methylation and polymorphism in the plants propagated in in vitro and in vivo conditions suggest the possibility of involvement of these fragments in the processes of regulating plant growth and development under prevailing growth conditions.

scholarly journals Effect of Boron and Calcium Foliar Sprays on Pollen Germination and Development, Fruit Set, Seed Development, and Berry Yield and Quality in Lowbush Blueberry (Vaccinium angustifolium Ait.)

1998 ◽  
Vol 123 (4) ◽  
pp. 524-531 ◽  
Author(s):  
Youzhi Chen ◽  
John M. Smagula ◽  
Walter Litten ◽  
Scott Dunham
Keyword(s):  
Pollen Germination ◽  
Fruit Set ◽  
Yield Reduction ◽  
Vaccinium Angustifolium ◽  
Foliar Sprays ◽  
Lowbush Blueberry ◽  
Berry Size ◽  
Number Of Seeds

In a managed field of native Vaccinium angustifolium Ait. clones, the effect of fall foliar sprays of B at 345 g·ha-1 and/or Ca at 3,450 g·ha-1 in remedying tissue deficiency of B varied among 12 clones, as seen in pollen germinability and on individual stems as seen in flower number, fruit set, and number of harvestable berries. With Ca applied alone, increased berry size did not overcome yield reduction due to fewer flowers and berries per stem. Berry diameter and mass correlated better to number of seeds of germinable size than to total number of seeds. Pollen germination averaged 17.4% on stigmata from untreated clones, and all three treatments (B, Ca, B + Ca) increased that average by 8%. More seeds per berry with the B-alone treatment implies more ovules fertilized when B deficiency is remedied. No relation was found between in vitro and in vivo pollen germination.

Laboratory efficacy and fungicide compatibility of Clonostachys rosea against Botrytis blight on lowbush blueberry

2013 ◽  
Vol 93 (4) ◽  
pp. 639-642 ◽  
Author(s):  
K. W. Reeh ◽  
G. C. Cutler
Keyword(s):  
Pest Management ◽  
Botrytis Cinerea ◽  
Endophytic Fungus ◽  
Field Research ◽  
Management Program ◽  
Clonostachys Rosea ◽  
Vaccinium Angustifolium ◽  
Lowbush Blueberry ◽  
Pre Treatment

Reeh, K. W. and Cutler, G. C. 2013. Laboratory efficacy and fungicide compatibility of Clonostachys rosea against Botrytis blight on lowbush blueberry. Can. J. Plant Sci. 93: 639–642. Lowbush blueberry (Vaccinium angustifolium) is an economically important crop. Clonostachys rosea is an endophytic fungus that can provide protection of plants from several pathogens, including Botrytis cinerea. It is unknown if C. rosea is able to colonize and protect V. angustifolium, and whether it is tolerant of fungicides commonly used for B. cinerea management in blueberry production. In a greenhouse experiment, pre-treatment of blueberry blossoms with C. rosea significantly reduced establishment of B. cinerea, but treatments after establishment of the pathogen were not effective. Clonostachys rosea demonstrated in vitro tolerance to the fungicide Switch®, but little or no tolerance to Pristine® and Maestro®. Our results are encouraging for additional field research examining the use of C. rosea as part of an integrated pest management program for B. cinerea control on lowbush blueberries.

Morphology, phenolic content and antioxidant capacity of lowbush blueberry (Vaccinium angustifolium Ait.) plants as affected by in vitro and ex vitro propagation methods

2013 ◽  
Vol 93 (6) ◽  
pp. 1001-1008 ◽  
Author(s):  
Juran C. Goyali ◽  
Abir U. Igamberdiev ◽  
Samir C. Debnath
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Phenolic Content ◽  
Flower Buds ◽  
Vaccinium Angustifolium ◽  
Growing Seasons ◽  
Lowbush Blueberry ◽  
Number Of Stems ◽  
Propagation Methods

Goyali, J. C., Igamberdiev, A. U. and Debnath, S. C. 2013. Morphology, phenolic content and antioxidant capacity of lowbush blueberry ( Vaccinium angustifolium Ait.) plants as affected by in vitro and ex vitro propagation methods. Can. J. Plant Sci. 93: 1001–1008. The lowbush blueberry (Vaccinium angustifolium Ait.), a commercially important crop in eastern Canada and the United States of America, is native to North America. It is one of the richest sources of antioxidant compounds and has been reported to be a potential component in reducing the incidence of cancers and cardiovascular diseases. The aim of this study is to evaluate the effect of propagation methods on morphological characters, phenolic content and antioxidant activity. A lowbush blueberry clone, QB 9C, and a cultivar, Fundy, were studied over two growing seasons after being propagated by conventional softwood cutting (SC) and by tissue culture (TC). Significant interactions among genotypes, propagation methods and growing seasons were observed for number of flower buds, total flavonoid (TFC) and proanthocyanidin (PAC) contents and antioxidant capacity. Propagation method interacted significantly with genotypes for the number of stems per plant and total phenolic (TPC) and chlorophyll contents, and with growing season for number of flower buds, TFC and PAC. TC plants produced higher number of stems and branches compared with SC plants. TPC, TFC, PAC, chlorophyll content and antioxidant activity were found in higher levels in the leaves of QB 9C compared with those of Fundy plants. The juvenile characteristics of TC plants which are triggered by growth hormones and readily available nutrients of culture media may be responsible for differences in morphological traits and antioxidant activity.

Polyclonal antisera to epacrid mycorrhizae and to Hymenoscyphus ericae display specificity

2000 ◽  
Vol 78 (7) ◽  
pp. 841-850
Author(s):  
R A Parry ◽  
C B McLean ◽  
M R Alderton ◽  
P J Coloe ◽  
A C Lawrie
Keyword(s):  
Mycorrhizal Fungi ◽  
Polyclonal Antibodies ◽  
Calluna Vulgaris ◽  
Immunogold Labelling ◽  
Fungal Endophyte ◽  
Cross Reactivity ◽  
Plant Roots ◽  
Hymenoscyphus Ericae ◽  
Ericoid Mycorrhizae ◽  
Polyclonal Antisera

Three polyclonal antisera produced in mice were used to investigate specificity and cross-reactivity between ericaceous and epacridaceous mycorrhizal fungi. One antiserum was to a culture of Hymenoscyphus ericae (Read) Korf and Kernan, the fungal endophyte of Calluna vulgaris (L.) Hull (Ericaceae). The other two were to peloton preparations from roots of Epacris impressa Labill. (Epacridaceae) from two sites (Cranbourne and Grampians) in Victoria, Australia. By immunofluorescence, all three antisera recognised H. ericae but not Oidiodendron griseum Roback, suggesting a serological relationship with the former endophyte. They also recognised 10 of the 12 fungal isolates tested, from mycorrhizal roots of E. impressa (Cranbourne), and all 4 isolates from Astroloma pinifolium (R. Br.) Benth. (Epacridaceae) (Grampians). Furthermore, none of the antisera recognised any of the nine common soil-inhabiting fungi selected for screening. Antisera recognised only unmelanized hyphae on epacrid and other plant roots taken from the wild. With plants from Cranbourne, all antisera except the Grampians antiserum recognised hyphae only on epacrid roots, demonstrating specificity. Hyphae on other plant roots were not recognised by any of the antisera. With plants from the Grampians, all antisera recognised some hyphae on both epacrid and other plant roots, except in two instances. The immunogold labelling indicates that the antisera are specific for fungi and do not recognise the plant. Since the fungal isolate forms true mycorrhizal structures, this suggests that there is a serological similarity between fungi forming epacrid mycorrhiza and those (H. ericae) forming ericoid mycorrhiza.Key words: ericoid mycorrhizae, Epacridaceae, polyclonal antibodies, immunofluorescence, immunogold.

IN VITRO SHOOT PRODUCTION OF LOWBUSH BLUEBERRY

1983 ◽  
Vol 63 (2) ◽  
pp. 467-472 ◽  
Author(s):  
JOHN J. FRETT ◽  
JOHN M. SMAGULA
Keyword(s):  
Tissue Culture ◽  
Indoleacetic Acid ◽  
Vaccinium Angustifolium ◽  
Total Length ◽  
Lowbush Blueberry ◽  
Shoot Production ◽  
Shoot Number ◽  
Number Of Shoots

The six most distal buds of lowbush blueberry (Vaccinium angustifolium Ait.) from shoots previously developed in tissue culture were transferred to media with 0, 7.5, 15, 22.5 or 30 mg/L 2, 6 (γ,γ-dimethylallyl-amino)-purine (2ip) and 0, 2, 4, 6, or 8 mg/L indoleacetic acid (IAA). The positional origin of the bud explant had little effect on number of shoots and no effect on total length of shoots produced. Shoot number and total length of shoots per tube increased with increasing 2ip concentrations. Increasing IAA concentrations decreased shoot numbers but had no effect on total length of shoots. Shoots were removed from culture after 8 wk and rooted in a peat, vermiculite and perlite medium. Percent rooting increased with increasing 2ip concentration and decreased with increasing IAA concentration in the proliferation medium.Key words: Lowbush blueberry, Vaccinium angustifolium

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