Microspectrophotometric evaluation of rosmarinic acid accumulation in single cultured plant cells

1984 ◽  
Vol 62 (11) ◽  
pp. 2278-2282 ◽  
Author(s):  
N. Chaprin ◽  
B. E. Ellis
Keyword(s):  
Rosmarinic Acid ◽  
Nuclear Dna ◽  
Cultured Cells ◽  
Nuclear Dna Content ◽  
Ultraviolet Region ◽  
Related Genome ◽  
Coleus Blumei ◽  
Acid Accumulation ◽  
Internal Concentration ◽  
Anchusa Officinalis

By use of microspectrophotometry in the ultraviolet region, rosmarinic acid was confirmed to be largely accumulated in the vacuoles of suspension cultured cells of Anchusa officinalis and Coleus blumei. The relative internal concentration of this metabolite in living cells can also be estimated with this technique. A comparison of the rosmarinic acid production and nuclear DNA content of individual cells suggests that the culture-related genome expansion may depress rosmarinic acid accumulation.

Rosmarinic Acid Synthesis in Transformed Callus Culture of Coleus blumei Benth

2004 ◽  
Vol 59 (7-8) ◽  
pp. 554-560 ◽  
Author(s):  
Nataša Bauer ◽  
Dunja Leljak-Levanic ◽  
Sibila Jelaska
Keyword(s):  
Growth Rate ◽  
Rosmarinic Acid ◽  
Acid Production ◽  
Acid Synthesis ◽  
Tissue Growth ◽  
Callus Growth ◽  
Tissue Mass ◽  
Coleus Blumei ◽  
Acid Accumulation

AbstractAgrobacteria mediated Coleus blumei tumour tissues were cultured in vitro on MS medium. Sixteen diversified transformed callus cultures were maintained for several years in the absence of plant growth regulators and antibiotics without affecting the growth rate. Rosmarinic acid was detected spectrophotometrically in all tissue lines but in different quantities. The highest rosmarinic acid accumulation detected was 11% of dry tissue mass. The relation between culture growth and rosmarinic acid production was investigated in three callus lines. The lines showed different rosmarinic acid accumulation in relation to their growth rate; it was either parallel or inversely related to the tissue growth. The effects of certain medium constituents on the callus growth and rosmarinic acid accumulation were examined in four tumour cell lines. Addition of 4% or 5% sucrose stimulated rosmarinic acid synthesis and decreased callus growth. Nitrogen reduction to one half or one quarter of initial concentration did not affect rosmarinic acid synthesis and decreased callus growth in three lines, while it increased rosmarinic acid accumulation and callus growth in one line. Addition of 0.1 mg/l Phe stimulated rosmarinic acid production in two lines but had little effect on the rosmarinic acid level in others. Rosmarinic acid production was significantly improved on modified macronutrients, where the Ac2 line produced 16.5 mg of rosmarinic acid per tube (0.2 g of dry wt) after being in culture for 35 days.

Prognostic value of nuclear DNA content in papillary and follicular thyroid cancer

1988 ◽  
Vol 12 (4) ◽  
pp. 503-507 ◽  
Author(s):  
Jaap F. Hamming ◽  
Lodewijk J. D. M. Schelfhout ◽  
Cees J. Cornelisse ◽  
Cornelis J. H. van de Velde ◽  
Bernard M. Goslings ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Dna Content ◽  
Prognostic Value ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Follicular Thyroid Cancer

scholarly journals Sequential production of gametes during meiosis in trypanosomes

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Lori Peacock ◽  
Chris Kay ◽  
Chloe Farren ◽  
Mick Bailey ◽  
Mark Carrington ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Cell Types ◽  
Tsetse Fly ◽  
Binucleate Cell ◽  
Insect Vector ◽  
Content Ratio ◽  
Different Cell Types ◽  
Meiosis I

AbstractMeiosis is a core feature of eukaryotes that occurs in all major groups, including the early diverging excavates. In this group, meiosis and production of haploid gametes have been described in the pathogenic protist, Trypanosoma brucei, and mating occurs in the salivary glands of the insect vector, the tsetse fly. Here, we searched for intermediate meiotic stages among trypanosomes from tsetse salivary glands. Many different cell types were recovered, including trypanosomes in Meiosis I and gametes. Significantly, we found trypanosomes containing three nuclei with a 1:2:1 ratio of DNA contents. Some of these cells were undergoing cytokinesis, yielding a mononucleate gamete and a binucleate cell with a nuclear DNA content ratio of 1:2. This cell subsequently produced three more gametes in two further rounds of division. Expression of the cell fusion protein HAP2 (GCS1) was not confined to gametes, but also extended to meiotic intermediates. We propose a model whereby the two nuclei resulting from Meiosis I undergo asynchronous Meiosis II divisions with sequential production of haploid gametes.

Estimation of nuclear DNA content by flow cytometry in fishes of the genus Xiphophorus

1989 ◽  
Vol 94 (3) ◽  
pp. 465-468 ◽  
Author(s):  
Terrence R. Tiersch ◽  
Robert W. Chandler ◽  
Klaus D. Kallman ◽  
Stephen S. Wachtel
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content
Sign in / Sign up

Export Citation Format

Share Document