Influence of the carbon source on growth and rosmarinic acid production in suspension cultures of Coleus blumei

1993 ◽  
Vol 34 (2) ◽  
pp. 183-190 ◽  
Author(s):  
Claudia Gertlowski ◽  
Maike Petersen
Keyword(s):  
Carbon Source ◽  
Rosmarinic Acid ◽  
Acid Production ◽  
Suspension Cultures ◽  
Coleus Blumei

The biosynthesis of rosmarinic acid in suspension cultures of Coleus blumei

1994 ◽  
pp. 171-179
Author(s):  
Maike Petersen ◽  
Elisabeth Häusler ◽  
Juliane Meinhard ◽  
Barbara Karwatzki ◽  
Claudia Gertlowski
Keyword(s):  
Rosmarinic Acid ◽  
Suspension Cultures ◽  
Coleus Blumei

Production of rosmarinic acid by cell-suspension cultures of Coleus blumei

1977 ◽  
Vol 64 (11) ◽  
pp. 585-586 ◽  
Author(s):  
M. H. Zenk ◽  
H. El-Shagi ◽  
B. Ulbrich
Keyword(s):  
Cell Suspension ◽  
Rosmarinic Acid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Coleus Blumei

Hydroxyphenylpyruvate Reductase From Cell Suspension Cultures Of Coleus Blumei Benth.

1991 ◽  
Vol 46 (5-6) ◽  
pp. 371-376 ◽  
Author(s):  
Elisabeth Häusler ◽  
Maike Petersen ◽  
August W. Alfermann
Keyword(s):  
Cell Suspension ◽  
Caffeic Acid ◽  
Rosmarinic Acid ◽  
Protein Concentration ◽  
Biosynthetic Pathway ◽  
Incubation Temperature ◽  
Cell Suspension Cultures ◽  
Maximal Activity ◽  
Suspension Cultures ◽  
Coleus Blumei

Cell suspension cultures of Coleus blumei Benth. producing high amounts of rosmarinic acid were used to study the biosynthetic pathway of this caffeic acid ester. One of the involved enzymes, the hydroxyphenylpyruvate reductase (HPPR), is characterized in this paper. HPPR catalyzes the NAD (P)H dependent reduction of p-hydroxyphenylpyruvate to p-hydroxyphenyllactate. The enzyme developed maximal activity at an incubation temperature of 37 °C and at a pH of 6.5 to 7.0. The reaction proceeded linearly for an incubation time of 60 min and up to a protein concentration of 0.2 mg per assay. As electron donor HPPR accepted NADH and NADPH with Km-values of 190 µm and 95 µm respectively. The enzyme reduced differently substituted hydroxyphenylpyruvates but not β-phenylpyruvate. The apparent Km-values for the various substrates were at 10 µM for p-hydroxyphenylpyruvate, at 130 µm for 3,4-dihydroxyphenylpyruvate and at 250 µM for 3-methoxy-4-hydroxyphenylpyruvate. HPPR was competitively inhibited by rosmarinic acid and pyruvate with Ki-values of 210 µM and 200 µM respectively. Caffeic acid, p-coumaric acid and cinnamic acid did not affect the enzyme activity but p-coumaroyl-CoA inhibited HPPR

scholarly journals Potential of Different Coleus blumei Tissues for Rosmarinic Acid Production

2015 ◽  
Vol 53 (1) ◽  
pp. 3-10 ◽  
Author(s):  
Nataša Bauer ◽  
◽  
Rosemary Vuković ◽  
Saša Likić ◽  
Sibila Jelaska ◽  
...  
Keyword(s):  
Rosmarinic Acid ◽  
Acid Production ◽  
Coleus Blumei

Rosmarinic Acid Synthesis in Transformed Callus Culture of Coleus blumei Benth

2004 ◽  
Vol 59 (7-8) ◽  
pp. 554-560 ◽  
Author(s):  
Nataša Bauer ◽  
Dunja Leljak-Levanic ◽  
Sibila Jelaska
Keyword(s):  
Growth Rate ◽  
Rosmarinic Acid ◽  
Acid Production ◽  
Acid Synthesis ◽  
Tissue Growth ◽  
Callus Growth ◽  
Tissue Mass ◽  
Coleus Blumei ◽  
Acid Accumulation

AbstractAgrobacteria mediated Coleus blumei tumour tissues were cultured in vitro on MS medium. Sixteen diversified transformed callus cultures were maintained for several years in the absence of plant growth regulators and antibiotics without affecting the growth rate. Rosmarinic acid was detected spectrophotometrically in all tissue lines but in different quantities. The highest rosmarinic acid accumulation detected was 11% of dry tissue mass. The relation between culture growth and rosmarinic acid production was investigated in three callus lines. The lines showed different rosmarinic acid accumulation in relation to their growth rate; it was either parallel or inversely related to the tissue growth. The effects of certain medium constituents on the callus growth and rosmarinic acid accumulation were examined in four tumour cell lines. Addition of 4% or 5% sucrose stimulated rosmarinic acid synthesis and decreased callus growth. Nitrogen reduction to one half or one quarter of initial concentration did not affect rosmarinic acid synthesis and decreased callus growth in three lines, while it increased rosmarinic acid accumulation and callus growth in one line. Addition of 0.1 mg/l Phe stimulated rosmarinic acid production in two lines but had little effect on the rosmarinic acid level in others. Rosmarinic acid production was significantly improved on modified macronutrients, where the Ac2 line produced 16.5 mg of rosmarinic acid per tube (0.2 g of dry wt) after being in culture for 35 days.

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