Fungal elicitor preparations and methyl jasmonate enhance rosmarinic acid accumulation in suspension cultures of Coleus blumei

1999 ◽  
Vol 18 (6) ◽  
pp. 485-489 ◽  
Author(s):  
E. Szabo ◽  
A. Thelen ◽  
M. Petersen
Keyword(s):  
Methyl Jasmonate ◽  
Rosmarinic Acid ◽  
Suspension Cultures ◽  
Fungal Elicitor ◽  
Coleus Blumei ◽  
Acid Accumulation

The biosynthesis of rosmarinic acid in suspension cultures of Coleus blumei

1994 ◽  
pp. 171-179
Author(s):  
Maike Petersen ◽  
Elisabeth Häusler ◽  
Juliane Meinhard ◽  
Barbara Karwatzki ◽  
Claudia Gertlowski
Keyword(s):  
Rosmarinic Acid ◽  
Suspension Cultures ◽  
Coleus Blumei

Microspectrophotometric evaluation of rosmarinic acid accumulation in single cultured plant cells

1984 ◽  
Vol 62 (11) ◽  
pp. 2278-2282 ◽  
Author(s):  
N. Chaprin ◽  
B. E. Ellis
Keyword(s):  
Rosmarinic Acid ◽  
Nuclear Dna ◽  
Cultured Cells ◽  
Nuclear Dna Content ◽  
Ultraviolet Region ◽  
Related Genome ◽  
Coleus Blumei ◽  
Acid Accumulation ◽  
Internal Concentration ◽  
Anchusa Officinalis

By use of microspectrophotometry in the ultraviolet region, rosmarinic acid was confirmed to be largely accumulated in the vacuoles of suspension cultured cells of Anchusa officinalis and Coleus blumei. The relative internal concentration of this metabolite in living cells can also be estimated with this technique. A comparison of the rosmarinic acid production and nuclear DNA content of individual cells suggests that the culture-related genome expansion may depress rosmarinic acid accumulation.

Production of rosmarinic acid by cell-suspension cultures of Coleus blumei

1977 ◽  
Vol 64 (11) ◽  
pp. 585-586 ◽  
Author(s):  
M. H. Zenk ◽  
H. El-Shagi ◽  
B. Ulbrich
Keyword(s):  
Cell Suspension ◽  
Rosmarinic Acid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Coleus Blumei

Differential Induction of Sesquiterpene Metabolism in Tobacco Cell Suspension Cultures by Methyl Jasmonate and Fungal Elicitor

2000 ◽  
Vol 381 (2) ◽  
pp. 285-294 ◽  
Author(s):  
Alejandra Mandujano-Chávez ◽  
Mark A. Schoenbeck ◽  
Lyle F. Ralston ◽  
Edmundo Lozoya-Gloria ◽  
Joseph Chappell
Keyword(s):  
Methyl Jasmonate ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Fungal Elicitor ◽  
Tobacco Cell ◽  
Differential Induction ◽  
Tobacco Cell Suspension

Hydroxyphenylpyruvate Reductase From Cell Suspension Cultures Of Coleus Blumei Benth.

1991 ◽  
Vol 46 (5-6) ◽  
pp. 371-376 ◽  
Author(s):  
Elisabeth Häusler ◽  
Maike Petersen ◽  
August W. Alfermann
Keyword(s):  
Cell Suspension ◽  
Caffeic Acid ◽  
Rosmarinic Acid ◽  
Protein Concentration ◽  
Biosynthetic Pathway ◽  
Incubation Temperature ◽  
Cell Suspension Cultures ◽  
Maximal Activity ◽  
Suspension Cultures ◽  
Coleus Blumei

Cell suspension cultures of Coleus blumei Benth. producing high amounts of rosmarinic acid were used to study the biosynthetic pathway of this caffeic acid ester. One of the involved enzymes, the hydroxyphenylpyruvate reductase (HPPR), is characterized in this paper. HPPR catalyzes the NAD (P)H dependent reduction of p-hydroxyphenylpyruvate to p-hydroxyphenyllactate. The enzyme developed maximal activity at an incubation temperature of 37 °C and at a pH of 6.5 to 7.0. The reaction proceeded linearly for an incubation time of 60 min and up to a protein concentration of 0.2 mg per assay. As electron donor HPPR accepted NADH and NADPH with Km-values of 190 µm and 95 µm respectively. The enzyme reduced differently substituted hydroxyphenylpyruvates but not β-phenylpyruvate. The apparent Km-values for the various substrates were at 10 µM for p-hydroxyphenylpyruvate, at 130 µm for 3,4-dihydroxyphenylpyruvate and at 250 µM for 3-methoxy-4-hydroxyphenylpyruvate. HPPR was competitively inhibited by rosmarinic acid and pyruvate with Ki-values of 210 µM and 200 µM respectively. Caffeic acid, p-coumaric acid and cinnamic acid did not affect the enzyme activity but p-coumaroyl-CoA inhibited HPPR

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