Growth and fungal persistence by Quercus rubra inoculated with ectomycorrhizal fungi and planted on a clear-cutting and strip mine

1984 ◽  
Vol 62 (8) ◽  
pp. 1571-1574 ◽  
Author(s):  
Peter R. Beckjord ◽  
Marla S. McIntosh
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Quercus Rubra ◽  
Root Systems ◽  
Fungal Species ◽  
Cenococcum Geophilum ◽  
Greenhouse Production ◽  
Clear Cut ◽  
Clear Cutting ◽  
Strip Mine ◽  
Mine Sites

Quercus rubra seedlings were grown in a greenhouse in media inoculated with and without vegetative hyphae of the ectomycorrhizal fungi Pisolithus tinctorius, Cenococcum geophilum, Scleroderma auranteum, and Rhizopogon nigrescens. Some seedlings were also inoculated with basidiospores of P. tinctorius and S. auranteum in the greenhouse. Rhizopogon nigrescens did not infect the seedlings during greenhouse production. Seedlings were planted and grown for two seasons on clear-cut and strip-mine sites. Some nonmycorrhizal seedlings were inoculated with basidiospores at planting. Seedling growth after one season on the clear-cut site was positive for all P. tinctorius, C. geophilum, and vegetative S. auranteum inoculations and significant differences in growth occurred among inoculation treatments. Root-core samples after one season showed that 80–100% of the seedlings on the clear-cut site had fungal persistence of assigned fungal species from all fungal inoculations except that no ectomycorrhizae were evident with seedlings field inoculated with basidiospores. Growth after two seasons on the clear-cut site was significantly different among treatments but not significantly different on the strip-mine site. Excavated root systems after two seasons showed that 0–100% of the seedlings on the clear-cut and strip-mine sites had fungal retention of assigned fungal species and ectomycorrhizae from basidiospore chip inoculations.

The effects of aluminum and calcium on the growth and nutrition of selected ectomycorrhizal fungi of jack pine

1991 ◽  
Vol 69 (8) ◽  
pp. 1691-1699 ◽  
Author(s):  
Mark H. R. Browning ◽  
Thomas C. Hutchinson
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Pinus Banksiana ◽  
Axenic Culture ◽  
Root Systems ◽  
Jack Pine ◽  
Fungal Species ◽  
Growth And Nutrition ◽  
Pine Stands ◽  
Al Treatment ◽  
Nutrient Solutions

The influence of Al and Ca on the growth, in axenic culture, of three ectomycorrhizal fungi associated with jack pine (Pinus banksiana Lamb.) was examined. Isolates of Hebeloma crustuliniforme (St. Amans) Quél., Rhizopogon rubescens (Tul.) Tulasne, and Suillus tomentosus (Kauff.) Singer, Snell & Dick were obtained from basidiocarps collected in jack pine stands of different ages. Basidiocarps and the soil surrounding them were analyzed for Al and Ca, as well as other elements. Each fungal species was grown for 4 weeks in nutrient solutions containing 37, 185, 370, or 740 μM Al combined in a factorial design with 25, 125, 250, or 500 μM Ca and maintained at pH 3.8. Growth of all three fungal species was reduced at 370 μM Al. Significant interaction was found between fungal species and Al treatment for all six elements measured in mycelial tissue. Stepwise increments in external Al concentration resulted in reduced mycelial concentrations of Ca, Mg, and K, and increased mycelial concentrations of Al, P, and Fe in H. crustuliniforme. High external Al levels resulted in reduced mycelial concentrations of all elements measured except Al and P in R. rubescens. In contrast, for S. tomentosus the same external Al levels increased the mycelial concentrations of all elements except Ca. The response of the three ectomycorrhizal fungi to Ca also differed. Growth of H. crustuliniforme was stimulated by stepwise increments in external Ca concentrations from 25 to 500 μM. Increments in calcium had no effect on the growth of R. rubescens. High external levels of Ca acted synergistically with high external Al concentrations to reduce growth by S. tomentosus. Unlike the other two species, the response of S. tomentosus to Al and Ca could not have been predicted from the soil and basidiocarp analyses. Alterations in Ca to Al ratios of soils may influence the succession of ectomycorrhizal fungi on conifer root systems. Key words: ectomycorrhizae, jack pine, aluminum, calcium, basidiocarps.

Intraspecific and interspecific growth variation of ectomycorrhizal fungi at different temperatures

1987 ◽  
Vol 65 (5) ◽  
pp. 869-875 ◽  
Author(s):  
Michael L. Cline ◽  
Raymond C. France ◽  
C. P. Patrick Reid
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Pure Culture ◽  
Mycelial Growth ◽  
Fungal Growth ◽  
Fungal Species ◽  
Pisolithus Tinctorius ◽  
The Other ◽  
Cenococcum Geophilum ◽  
Thelephora Terrestris ◽  
Temperature Optima

Geographically distinct isolates of the ectomycorrhizal fungi Pisolithus tinctorius, Cenococcum geophilum, Thelephora terrestris, and Suillus granulatus showed definite temperature optima for growth in pure culture. Temperatures promoting the greatest fungal growth varied interspecifically and intraspecifically over the temperature treatments of 16, 21, 27, 32, and 38 °C. Of the four fungal species, Pisolithus tinctorius exhibited the greatest growth at the higher temperature treatments, with growth optima for all isolates occurring between 21 and 32 °C. The maximum temperature for growth of Cenococcum geophilum was 27 °C, with optimal mycelial growth occurring between 16 and 27 °C, depending upon the particular isolate considered. Suillus granulatus showed greatest mycelial growth at 27 °C and below, with temperature optima for the different isolates ranging from 16 to 32 °C. The pure-culture growth of Thelephora terrestris was high relative to the other fungal species examined with growth optima between 21 and 27 °C. The degree of intraspecific variation of mycelial growth in response to temperature was high for all fungal species, indicating the existence of physiologically distinct genotypes. Attempts to relate fungal growth performance to geographic origin of isolate showed a trend for Pisolithus tinctorius. However, no such relationships were apparent for the other species.

The influence of clear-cutting on ectomycorrhizal fungus diversity in a lodgepole pine (Pinus contorta) stand, Yellowstone National Park, Wyoming, and Gallatin National Forest, Montana

2000 ◽  
Vol 78 (2) ◽  
pp. 149-156 ◽  
Author(s):  
Kristin B Byrd ◽  
V Thomas Parker ◽  
Detlev R Vogler ◽  
Ken W Cullings
Keyword(s):  
Species Richness ◽  
Species Composition ◽  
Yellowstone National Park ◽  
National Park ◽  
Pinus Contorta ◽  
Molecular Techniques ◽  
Soil Core ◽  
Cenococcum Geophilum ◽  
Clear Cut ◽  
Clear Cutting

Effects of clear-cutting on the ectomycorrhizal (EM) fungus community in a Pinus contorta Dougl. ex Loud. forest near Yellowstone National Park, Wyoming, U.S.A., were assessed using molecular techniques. Samples were taken by soil core in both undisturbed and clear-cut sites by randomized block design. Species overlap was compared between clear-cut and undisturbed sites and ascomycete-basidiomycete ratio was determined, using PCR-RFLP methods. Fifty species of EM fungi were detected in the clear-cut sites, the most common being Cenococcum geophilum Fr., Suillus sp., a member of the suilloid group, a Russulaceae species, and a Thelephoraceae species. Sixty-six species were detected in the undisturbed sites, which were dominated by a Suilloid species, a Tricholomataceae species, Cortinarius sp., and Cenococcum geophilum. Species composition in the clear-cut sites differed significantly from that in the undisturbed sites (P = 0.0001). However, 9 of the 14 species most commonly found in the clear-cut sites were also found in the undisturbed sites, but in much lower abundance, while species rank curves of both stand types mirrored each other. There were no significant differences in species richness, root-tip abundance, or ascomycete-basidiomycete ratio between the clear-cut and undisturbed sites. However, species richness was lower in the clear-cut sites than in the undisturbed sites. An overall loss of species richness after clear-cutting and significant changes in species composition indicate that clear-cutting can negatively alter the EM fungal community, and this may have profound effects on ecosystem function.Key words: ectomycorrhizae, community structure, clear-cutting, molecular techniques.

Responses of jack pine and black spruce seedlings to inoculation with selected species of ectomycorrhizal fungi

1991 ◽  
Vol 21 (5) ◽  
pp. 701-706 ◽  
Author(s):  
Mark H. R. Browning ◽  
Roy D. Whitney
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Black Spruce ◽  
Weight Ratio ◽  
Dry Weight ◽  
Root Systems ◽  
Jack Pine ◽  
Fungal Species ◽  
Short Root ◽  
Root Dry Weight ◽  
Aseptic Culture

Seedlings of jack pine (Pinusbanksiana Lamb.) and black spruce (Piceamariana (Mill.) B.S.P.) were inoculated with liquid cultures of fragmented hyphae of 10 species of ectomycorrhizal fungi and grown in either aseptic culture tubes for 20 weeks or nonaseptic pots for 16 weeks. Seedlings in pots received more total nutrients and produced larger root systems than those in tubes. Four of the fungi produced higher levels of colonization associated with these larger root systems. Short-root density of black spruce was increased compared with that of uninoculated seedlings by inoculation with Laccariabicolor (Maire) Orton, Hebelomacylindrosporum Romagnési, or Thelephoraterrestris Ehrh. ex Fr. Colonization of jack pine by Rhizopogonrubescens (Tul.) Tulasne, L. bicolor, H. cylindrosporum, or Pisolithustinctorius (Pers.) Coker & Couch resulted in greater short-root densities than in uninoculated controls. Inoculation of potted black spruce with Laccariaproxima Boudier resulted in larger shoot weights and those inoculated with H. cylindrosporum had smaller root weights than did uninoculated seedlings. Both fungi resulted in increased shoot/root dry-weight ratios in black spruce. In contrast with black spruce, seedling weights were not affected by inoculation of jack pine with the same fungal species. However, inoculation of jack pine with R. rubescens resulted in a significantly higher shoot/root dry-weight ratio.

Planting Betula pendula on pine sites infested by Heterobasidion annosum: disease transfer, silvicultural evaluation, and community of wood-inhabiting fungi

2004 ◽  
Vol 34 (1) ◽  
pp. 120-130 ◽  
Author(s):  
Vaidotas Lygis ◽  
Rimvydas Vasiliauskas ◽  
Jan Stenlid
Keyword(s):  
Root Rot ◽  
Forest Regeneration ◽  
Betula Pendula ◽  
Root Systems ◽  
Fungal Species ◽  
Heterobasidion Annosum ◽  
Strong Impact ◽  
Fungal Isolation ◽  
Somatic Incompatibility ◽  
Forest Sites

Persistence of the root rot pathogen Heterobasidion annosum (Fr.) Bref. s.s. on infested areas and its transfer to a forest regeneration was studied in three forest sites in eastern Lithuania. The sites represented H. annosum disease centres in Pinus sylvestris L. stands, which were clear-felled and replanted with Betula pendula Roth 25 years prior to our study. Fungal isolation from trees and stumps on each site was performed on both replanted B. pendula and surrounding P. sylvestris from the previous generation. Low productivity of B. pendula stands (45.0–76.1 m3·ha–1), high mortality rates, and comparatively low vigor of trees (measured as crown densities) indicated a strong impact of root rot. Based on somatic incompatibility tests, we detected large spreading areas of clones of H. annosum (up to 48 m across) and old (35- to 40-year-old) clonal individuals. Territorial clones covered areas that encompassed both previous stands of P. sylvestris and current stands of B. pendula. Our results showed that H. annosum is able to persist in root systems of diseased trees for decades and readily attack birch replanted on infested sites. In addition, a total of 83 fungal species (out of 398 isolates) was found as a result of sampling 508 B. pendula, 49 P. sylvestris, 21 Juniperus communis L., and 1 Salix cinerea L. trees.

Evaluation of planting sites common to a southeast Alaska clear-cut. IV. Nutrient levels in ectomycorrhizal Sitka spruce seedlings

1987 ◽  
Vol 17 (4) ◽  
pp. 340-345 ◽  
Author(s):  
Roy C. Sidle ◽  
Charles G. Shaw III
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Mineral Soil ◽  
Sitka Spruce ◽  
Southeast Alaska ◽  
Clear Cut ◽  
The North ◽  
Rotten Wood ◽  
North Side ◽  
Foliar Concentrations ◽  
Nutritional Benefits

Sitka spruce (Piceasitchensis (Bong.) Carr.) seedlings colonized by the ectomycorrhizal fungi Laccarialaccata (Scop. ex Fr.) Berk and Br., Hebelomacrustuliniforme (Bull, ex St. Amans) Quel., or Cenococcumgeophilum Fr. and a noninoculated control were planted on rotten wood, undisturbed duff, undisturbed duff on the north side of large stumps, and exposed mineral soil in an area recently clear-cut on Chichagof Island, southeast Alaska. Uptake and concentrations of N, P, K, Ca, Mg, and several micronutrients in foliage, stems, and roots were determined 2 years after outplanting. There were no significant interactions between fungal treatments and microsite types for uptake or concentration of any nutrient. Uptake and concentrations of N and P in foliage and roots did not differ significantly among fungal treatments. Foliar K and Cu concentrations were significantly higher in control seedlings than in those with L. laccata ectomycorrhizae. Calcium was the only nutrient analyzed that was present in higher concentrations in seedlings inoculated with H. crustuliniforme than in controls. Concentrations of foliar P, Mn, and Zn were significantly greater in seedlings grown on undisturbed duff (protected north side) than in seedlings grown on exposed mineral soil. However, foliar concentrations of all nutrients tested were not significantly different between seedlings grown on undisturbed duff (unprotected sites) and those grown on mineral soil. Seedlings grown on rotten wood had significantly greater concentrations of foliar Ca than seedlings grown on either exposed mineral soil or undisturbed duff. The on-site colonization of control seedlings after outplanting indicates that resident ectomycorrhizal fungi readily establish and appear to provide equal if not improved nutrient benefits compared to inoculated seedlings. Greater nutritional benefits were derived by planting on certain microsite types (duff and protected duff) than by inoculating with specific ectomycorrhizal fungi.

Changes in small mammal communities after logging in north-central Ontario

1983 ◽  
Vol 61 (5) ◽  
pp. 970-980 ◽  
Author(s):  
Arthur M. Martell
Keyword(s):  
Small Mammals ◽  
Small Mammal ◽  
Vegetative Cover ◽  
North Central ◽  
Clear Cut ◽  
Clear Cutting ◽  
Small Mammal Community ◽  
Small Mammal Communities ◽  
Mammal Community ◽  
Mammal Communities

Changes in small mammal communities following logging were monitored in clear-cut and strip-cut upland black spruce (Picea mariana) stands and in selectively cut mixed wood stands in north-central Ontario. Clear-cutting and subsequent scarification essentially eliminated the vegetative cover. Much of the ground cover recovered within 5 years and shrubs within 12 years, but mosses and lichens took much longer. The small mammal community in both clear-cut and strip-cut stands changed over the first three years after logging from one dominated by southern red-backed voles (Clethrionomys gapperi) to one dominated by deer mice (Peromyscus maniculatus) and then remained relatively stable for up to 13 years after harvest. That shift was not apparent in selectively cut mixed wood stands where the composition of the small mammal community was similar between uncut stands and stands 4–23 years after harvest. There was relatively little change in total numbers of small mammals after logging. In general, the diversity and evenness of small mammals increased or remained stable in the first 1–3 years following harvest, decreased on older (3–16 years) cuts, and then increased to values similar to those in uncut stands on the oldest (19–23 years) cuts.

scholarly journals Natural Regeneration of the Tree Stand in the Bilberry Spruce Forest—Clear-Cutting Ecotone Complex in the First Post-Logging Decade

Forests ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1542
Author(s):  
Nadezhda V. Genikova ◽  
Viktor N. Mamontov ◽  
Alexander M. Kryshen ◽  
Vladimir A. Kharitonov ◽  
Sergey A. Moshnikov ◽  
...  
Keyword(s):  
Natural Regeneration ◽  
Forest Canopy ◽  
Forest Cover ◽  
Forest Type ◽  
Ground Cover ◽  
Woody Species ◽  
Forest Edge ◽  
Tree Canopy ◽  
Clear Cut ◽  
Clear Cutting

Bilberry spruce forests are the most widespread forest type in the European boreal zone. Limiting the clear-cuttings size leads to fragmentation of forest cover and the appearance of large areas of ecotone complexes, composed of forest (F), a transition from forest to the cut-over site under tree canopy (FE), a transition from forest to the cut-over site beyond tree canopy (CE), and the actual clear-cut site (C). Natural regeneration of woody species (spruce, birch, rowan) in the bilberry spruce stand—clear-cut ecotone complex was studied during the first decade after logging. The effects produced by the time since cutting, forest edge aspect, and the ground cover on the emergence and growth of trees and shrubs under forest canopy and openly in the clear-cut were investigated. Estimating the amount and size of different species in the regeneration showed FE and CE width to be 8 m—roughly half the height of first-story trees. Typical forest conditions (F) feature a relatively small amount of regenerating spruce and birch. The most favorable conditions for natural regeneration of spruce in the clear-cut—mature bilberry spruce stand ecotone are at the forest edge in areas of transition both towards the forest and towards the clear-cut (FE and CE). Clear-cut areas farther from the forest edge (C) offer an advantage to regenerating birch, which grows densely and actively in this area.

Spider assemblage structure and functional diversity patterns in clear-cut, logged, and undisturbed areas in a large Atlantic Forest remnant

2020 ◽  
Vol 50 (7) ◽  
pp. 608-614
Author(s):  
Ronei Baldissera ◽  
Suiane Oliveira de Quadros ◽  
Gabriela Galeti ◽  
Everton Nei Lopes Rodrigues ◽  
Luan M.V. Lazzarotto ◽  
...  
Keyword(s):  
Atlantic Forest ◽  
Species Conservation ◽  
Basal Area ◽  
Selective Logging ◽  
Canopy Openness ◽  
Forest Fragment ◽  
Clear Cut ◽  
Clear Cutting ◽  
Functional Richness ◽  
Forest Remnant

Habitat loss is one of the main consequences of landscape transformation by humans. Monitoring biodiversity changes in areas under different management strategies is fundamental for species conservation. Our study is the first to assess the role of forest disturbance history on spider (Araneae) biodiversity in the westernmost portion of the Atlantic Forest. We analyzed taxonomic and functional aspects of spider assemblages in understories in a large forest fragment in southwestern Brazil. Spiders were sampled in five 30 m × 5 m plots over three seasons in three areas with different management histories: clear-cutting, selective logging, or native plots. We also characterized tree basal area, tree density, and canopy openness. The clear-cut plots showed more canopy openness and low habitat heterogeneity due to the high density of one pioneer native tree species. Forest structure in selective logging and native plots was similar. Spider richness, abundance, and functional richness were affected only by the season. Species composition also differed among the areas depending on the season. The abundance of web-building species was mainly associated with clear-cut areas in winter and spring. These results highlight the importance of natural regeneration in the Atlantic Forest after disturbance for the conservation of regional spider biodiversity.

The potential for woody understory plants to provide refuge for ectomycorrhizal inoculum at an interior Douglas-fir forest after clear-cut logging

2001 ◽  
Vol 31 (4) ◽  
pp. 711-721 ◽  
Author(s):  
Shannon M Hagerman ◽  
Stacey M Sakakibara ◽  
Daniel M Durall
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Ectomycorrhizal Fungi ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Douglas Fir ◽  
Molecular Analyses ◽  
Clear Cut ◽  
Fungal Inoculum ◽  
Pseudostuga Menziesii ◽  
Restriction Fragment Length

Clear-cut logging can decrease the amount of inoculum for some ectomycorrhizal fungi. Ectomycorrhizal plants that are not selected for harvest (refuge plants) may, therefore, be important for the maintenance of ectomycorrhizal fungal inoculum in clearcuts following logging. The purpose of this study was to identify refuge plants that could provide ectomycorrhizal fungal inoculum for outplanted seedlings. The ectomycorrhizal status of selected plants was assessed in 1.6-ha clearcuts and in adjacent forest. Over 3 years, 17 and 14 morphotypes were described for Pseudostuga menziesii (Mirb.) Franco (Douglas-fir) and Arctostaphylos uva-ursi (L.) Spreng, respectively. Ten morphotypes (six of these confirmed with restriction fragment length polymorphism patterns) were shared by both species. Anatomical and molecular analyses revealed that, for the morphotypes observed, ectomycorrhizal fungi formed ectomycorrhizal anatomy when associated with Douglas-fir and arbutoid anatomy with A. uva-ursi. Because advanced regeneration seedlings of Douglas-fir and A. uva-ursi are well distributed throughout this site, these two species have a high potential to provide ectomycorrhizal fungal inoculum for outplanted seedlings. There was no difference in mycorrhizal richness between A. uva-ursi plants sampled from the clearcut and forest in the latter 2 years of the study. Conversely, the richness of ectomycorrhizae associated with Douglas-fir sampled from the forest was significantly greater than for seedlings sampled from the clearcuts.

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