The DNA content of the mycelial nuclei of rust fungi grown on artificial media

1984 ◽  
Vol 62 (2) ◽  
pp. 189-194 ◽  
Author(s):  
Yasuyuki Tetsuka ◽  
Katsuhiko Ando ◽  
Yuichi Yamaoka ◽  
Keizo Katsuya
Keyword(s):  
Propidium Iodide ◽  
Dna Content ◽  
Rust Fungi ◽  
Puccinia Recondita ◽  
Two Cultures ◽  
Propidium Iodide Staining ◽  
Germ Tubes ◽  
Nuclear Condition

The nuclear condition of vegetative mycelia of several races of Puccinia recondita f. sp. tritici, P. graminis f. sp. tritici, P. coronata f. sp. avenae, and Gymnosporangium asiaticum was determined by propidium iodide staining. Cultures originating from the uredinial stage of P. recondita f. sp. tritici, from the uredinial stage of P. coronata f. sp. avenae, and from the spermogonial stage of Gymnosporangium asiaticum were all predominantly monokaryotic. The dikaryotic condition was observed in two cultures from the uredinial stage of P. graminis f. sp. tritici and P. recondita f. sp. tritici, in one culture from the aecial stage of Gy. asiaticum, and in germ tubes from uredospores of Puccinia spp. Measurements of the DNA content indicated monokaryotic diploid cultures and dikaryotic haploid cultures in the Puccinia species. In Gy. asiaticum, all nuclei were haploid, but the nuclei had twice as much DNA as haploid nuclei in the Puccinia spp.

Propidium iodide staining of cytoautoradiographic preparations for the simultaneous determination of DNA content and grain count

1985 ◽  
Vol 17 (11) ◽  
pp. 1259-1270 ◽  
Author(s):  
P. A. Giordano ◽  
G. Mazzini ◽  
A. Riccardi ◽  
C. M. Montecucco ◽  
G. Ucci ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Propidium Iodide ◽  
Dna Content ◽  
Propidium Iodide Staining

Tyrosine Kinase Inhibitor-Mediated Apoptosis Is Potentiated by FasL and Increased with IFN-α in Chronic Myeloid Leukemia

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 4428-4428
Author(s):  
Laurence Legros ◽  
Nathalie Ebran ◽  
Jean-Michel Karsenti ◽  
Faezeh Legrand ◽  
Lionel Mannone ◽  
...  
Keyword(s):  
Cell Line ◽  
Propidium Iodide ◽  
Dna Content ◽  
Propidium Iodide Staining ◽  
Potentiation Effect ◽  
Imatinib Resistant ◽  
Cd34 Cells ◽  
The Mean ◽  
Cellular Dna

Abstract Abstract 4428 The Fas death receptor (CD95/TNFSFR6) conveys death and non-death signals through binding to its cognate ligand, FasL (CD95L)1,2. Fas is expressed constitutively in CD34+ cells of patients with chronic myeloid leukemia3. In order to explore the implication of Fas in CML patient's response, we aimed at analyzing expression and function of Fas in the imatinib-sensitive human Bcr-abl+ Fas+ AR 230S cell line, and in its imatinib-resistant counterpart AR 230R. We analysed the Fas-mediated apoptosis of AR230S and showed that not only imatinib (Figure 1) but also nilotinib and dasatinib (data not shown) potentiate Fas-L mediated apoptosis. This potentiation is dose dependent (Figure 2). Interestingly, there is no potentiation of TKI-mediated apoptosis by FasL in the imatinib-resistant counterpart AR 230R which is Fas negative. The Fas knockdown with a lentivirus-expressing a FAS-shRNA in AR 230S was associated with a disappearance of potentiation effect, whereas the up regulation of Fas expression in AR230 S cell line using lentivirus-expressing human Fas (hFas) induced an emphasized potentiation effect confirming the pivotal role of Fas in the potentiation of TKI-mediated apoptosis. Notably, IFN-α is able to enhance expression of Fas on CD34+ cells from CML patients3. We thus explored the action of IFN-α on the Fas potentiation of TKI-mediated apoptosis. We observed that preincubation with IFN-α increased potentiation in a dose-dependant manner (Data not shown). In conclusion, our preliminary results demonstrate the pivotal role of Fas in the potentiation of TKI-mediated apoptosis and highlight the role of IFN-α in the design of CML treatments. Figure 1: Percentage of FasL-mediated apoptosis in AR230 cell line. Apoptosis induced by FasL in presence or in absence of imatinib was analyzed by propidium iodide staining of cellular DNA content and shown as percentage of sub-G1 to the whole population. Figure 1:. Percentage of FasL-mediated apoptosis in AR230 cell line. Apoptosis induced by FasL in presence or in absence of imatinib was analyzed by propidium iodide staining of cellular DNA content and shown as percentage of sub-G1 to the whole population. Figure 2: Dose-dependentof FasL mediated apoptosis in absence or in combination with imatinib. Apoptosis induced by FasL in presence or in absence of imatinib analyzed by propidium iodide staining of cellular DNA content and shown as percentage of sub-G1 to the whole population. Each point represents the mean ± S.E.M. of 3 experiments. Figure 2:. Dose-dependentof FasL mediated apoptosis in absence or in combination with imatinib. Apoptosis induced by FasL in presence or in absence of imatinib analyzed by propidium iodide staining of cellular DNA content and shown as percentage of sub-G1 to the whole population. Each point represents the mean ± S.E.M. of 3 experiments. Disclosures: Legros: Novartis: Research Funding, Speakers Bureau; BMS: Speakers Bureau; Celgene: Speakers Bureau.

Increased Lipoxygenase Activity is Involved in the Hypersensitive Response of Wheat Leaf Cells Infected with A virulent Rust Fungi or Treated with Fungal Elicitor

1986 ◽  
Vol 41 (5-6) ◽  
pp. 559-563 ◽  
Author(s):  
Carlos A. Ocampo ◽  
Bruno Moerschbacher ◽  
Hans J. Grambow
Keyword(s):  
Hypersensitive Response ◽  
Hypersensitive Reaction ◽  
Rust Fungi ◽  
Lipoxygenase Activity ◽  
Puccinia Graminis ◽  
Compatible Interaction ◽  
Wheat Leaf ◽  
Wheat Leaves ◽  
Germ Tubes ◽  
Wheat Rust

The hypersensitive reaction in incompatible wheat-rust interactions is characterized by an increase in lipoxygenase activity detectable as early as 28 h after penetration of the pathogen. In contrast, lipoxygenase activity in the compatible interaction did not increase until the onset of sporulation.Lipoxygenase activity also increased following treatment of wheat leaves with an elicitor fraction from germ tubes of Puccinia graminis tritici.

High‐Throughput Cytotoxicity Screening by Propidium Iodide Staining

2007 ◽  
Vol 41 (1) ◽  
Author(s):  
Bruce S. Edwards ◽  
Irena Ivnitski‐Steele ◽  
Susan M. Young ◽  
Virginia M. Salas ◽  
Larry A. Sklar
Keyword(s):  
High Throughput ◽  
Propidium Iodide ◽  
Propidium Iodide Staining

scholarly journals Data-Driven Compensation for Flow Cytometry of Solid Tissues

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Nickolaas Maria van Rodijnen ◽  
Math Pieters ◽  
Sjack Hoop ◽  
Marius Nap
Keyword(s):  
Flow Cytometry ◽  
Propidium Iodide ◽  
Dna Content ◽  
Flow Cytometer ◽  
Data Driven ◽  
Color Flow ◽  
Operator Experience ◽  
Solid Tissues

Propidium Iodide is a fluorochrome that is used to measure the DNA content of individual cells, taken from solid tissues, with a flow cytometer. Compensation for spectral cross-over of this fluorochrome still leads to compensation results that are depending on operator experience. We present a data-driven compensation (DDC) algorithm that is designed to automatically compensate combined DNA phenotype flow cytometry acquisitions. The generated compensation values of the DDC algorithm are validated by comparison with manually determined compensation values. The results show that (1) compensation of two-color flow cytometry leads to comparable results using either manual compensation or the DDC method; (2) DDC can calculate sample-specific compensation trace lines; (3) the effects of two different approaches to calculate compensation values can be visualized within one sample. We conclude that the DDC algorithm contributes to the standardization of compensation for spectral cross-over in flow cytometry of solid tissues.

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