Propidium iodide staining of cytoautoradiographic preparations for the simultaneous determination of DNA content and grain count

1985 ◽  
Vol 17 (11) ◽  
pp. 1259-1270 ◽  
Author(s):  
P. A. Giordano ◽  
G. Mazzini ◽  
A. Riccardi ◽  
C. M. Montecucco ◽  
G. Ucci ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Propidium Iodide ◽  
Dna Content ◽  
Propidium Iodide Staining

The DNA content of the mycelial nuclei of rust fungi grown on artificial media

1984 ◽  
Vol 62 (2) ◽  
pp. 189-194 ◽  
Author(s):  
Yasuyuki Tetsuka ◽  
Katsuhiko Ando ◽  
Yuichi Yamaoka ◽  
Keizo Katsuya
Keyword(s):  
Propidium Iodide ◽  
Dna Content ◽  
Rust Fungi ◽  
Puccinia Recondita ◽  
Two Cultures ◽  
Propidium Iodide Staining ◽  
Germ Tubes ◽  
Nuclear Condition

The nuclear condition of vegetative mycelia of several races of Puccinia recondita f. sp. tritici, P. graminis f. sp. tritici, P. coronata f. sp. avenae, and Gymnosporangium asiaticum was determined by propidium iodide staining. Cultures originating from the uredinial stage of P. recondita f. sp. tritici, from the uredinial stage of P. coronata f. sp. avenae, and from the spermogonial stage of Gymnosporangium asiaticum were all predominantly monokaryotic. The dikaryotic condition was observed in two cultures from the uredinial stage of P. graminis f. sp. tritici and P. recondita f. sp. tritici, in one culture from the aecial stage of Gy. asiaticum, and in germ tubes from uredospores of Puccinia spp. Measurements of the DNA content indicated monokaryotic diploid cultures and dikaryotic haploid cultures in the Puccinia species. In Gy. asiaticum, all nuclei were haploid, but the nuclei had twice as much DNA as haploid nuclei in the Puccinia spp.

scholarly journals Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

1975 ◽  
Vol 66 (1) ◽  
pp. 188-193 ◽  
Author(s):  
A Krishan
Keyword(s):  
Propidium Iodide ◽  
Minimal Amount ◽  
Hypotonic Solution ◽  
Nuclear Chromatin ◽  
Propidium Iodide Staining ◽  
Mammalian Cell Cycle ◽  
Rapid Flow ◽  
Present Technique ◽  
Rnase Digestion

A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells stained by this method are identical to those generated after fixation and RNase digestion. In contrast to some earlier described methods, the present technique is rapid (5 min of processing), requires a minimal amount of material, and avoids formation of cell clumps.

Tyrosine Kinase Inhibitor-Mediated Apoptosis Is Potentiated by FasL and Increased with IFN-α in Chronic Myeloid Leukemia

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 4428-4428
Author(s):  
Laurence Legros ◽  
Nathalie Ebran ◽  
Jean-Michel Karsenti ◽  
Faezeh Legrand ◽  
Lionel Mannone ◽  
...  
Keyword(s):  
Cell Line ◽  
Propidium Iodide ◽  
Dna Content ◽  
Propidium Iodide Staining ◽  
Potentiation Effect ◽  
Imatinib Resistant ◽  
Cd34 Cells ◽  
The Mean ◽  
Cellular Dna

Abstract Abstract 4428 The Fas death receptor (CD95/TNFSFR6) conveys death and non-death signals through binding to its cognate ligand, FasL (CD95L)1,2. Fas is expressed constitutively in CD34+ cells of patients with chronic myeloid leukemia3. In order to explore the implication of Fas in CML patient's response, we aimed at analyzing expression and function of Fas in the imatinib-sensitive human Bcr-abl+ Fas+ AR 230S cell line, and in its imatinib-resistant counterpart AR 230R. We analysed the Fas-mediated apoptosis of AR230S and showed that not only imatinib (Figure 1) but also nilotinib and dasatinib (data not shown) potentiate Fas-L mediated apoptosis. This potentiation is dose dependent (Figure 2). Interestingly, there is no potentiation of TKI-mediated apoptosis by FasL in the imatinib-resistant counterpart AR 230R which is Fas negative. The Fas knockdown with a lentivirus-expressing a FAS-shRNA in AR 230S was associated with a disappearance of potentiation effect, whereas the up regulation of Fas expression in AR230 S cell line using lentivirus-expressing human Fas (hFas) induced an emphasized potentiation effect confirming the pivotal role of Fas in the potentiation of TKI-mediated apoptosis. Notably, IFN-α is able to enhance expression of Fas on CD34+ cells from CML patients3. We thus explored the action of IFN-α on the Fas potentiation of TKI-mediated apoptosis. We observed that preincubation with IFN-α increased potentiation in a dose-dependant manner (Data not shown). In conclusion, our preliminary results demonstrate the pivotal role of Fas in the potentiation of TKI-mediated apoptosis and highlight the role of IFN-α in the design of CML treatments. Figure 1: Percentage of FasL-mediated apoptosis in AR230 cell line. Apoptosis induced by FasL in presence or in absence of imatinib was analyzed by propidium iodide staining of cellular DNA content and shown as percentage of sub-G1 to the whole population. Figure 1:. Percentage of FasL-mediated apoptosis in AR230 cell line. Apoptosis induced by FasL in presence or in absence of imatinib was analyzed by propidium iodide staining of cellular DNA content and shown as percentage of sub-G1 to the whole population. Figure 2: Dose-dependentof FasL mediated apoptosis in absence or in combination with imatinib. Apoptosis induced by FasL in presence or in absence of imatinib analyzed by propidium iodide staining of cellular DNA content and shown as percentage of sub-G1 to the whole population. Each point represents the mean ± S.E.M. of 3 experiments. Figure 2:. Dose-dependentof FasL mediated apoptosis in absence or in combination with imatinib. Apoptosis induced by FasL in presence or in absence of imatinib analyzed by propidium iodide staining of cellular DNA content and shown as percentage of sub-G1 to the whole population. Each point represents the mean ± S.E.M. of 3 experiments. Disclosures: Legros: Novartis: Research Funding, Speakers Bureau; BMS: Speakers Bureau; Celgene: Speakers Bureau.

A general computer program for the extended plate overlap algorithm

1978 ◽  
Vol 48 ◽  
pp. 287-293 ◽  
Author(s):  
Chr. de Vegt ◽  
E. Ebner ◽  
K. von der Heide
Keyword(s):  
Computer Program ◽  
Simultaneous Determination ◽  
General Computer Program ◽  
General Computer

In contrast to the adjustment of single plates a block adjustment is a simultaneous determination of all unknowns associated with many overlapping plates (star positions and plate constants etc. ) by one large adjustment. This plate overlap technique was introduced by Eichhorn and reviewed by Googe et. al. The author now has developed a set of computer programmes which allows the adjustment of any set of contemporaneous overlapping plates. There is in principle no limit for the number of plates, the number of stars, the number of individual plate constants for each plate, and for the overlapping factor.

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