Regeneration of peanut (Arachis hypogaea) plantlets by in vitro culture of immature leaves

1981 ◽  
Vol 59 (5) ◽  
pp. 826-830 ◽  
Author(s):  
L. A. Mroginski ◽  
K. K. Kartha ◽  
J. P. Shyluk
Keyword(s):  
In Vitro Culture ◽  
Arachis Hypogaea ◽  
Environmental Conditions ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Bud Regeneration ◽  
Arachis Hypogaea L ◽  
Murashige And Skoog Medium ◽  
Germinated Seeds

The in vitro regeneration of buds, shoots, and roots from immature leaves of 3- to 5-day-old peanut (Arachis hypogaea L. cv. Colorado Manfredi) seedlings was studied under defined nutritional, hormonal, and environmental conditions. The first two leaves (2–5 mm in length) removed from aseptically germinated seeds were cultured on Murashige and Skoog medium containing vitamins as in B5 medium and 0.8% agar, supplemented with 12 combinations of naphthaleneacetic acid (NAA) (0.01 to 4 mg/L) and benzyladenine (BA) (1 and 3 mg/L). Bud regeneration occurred in all hormone combinations, but the maximum number of buds was regenerated at a concentration of 1 mg/L each of NAA and BA. Although bud regeneration was maximum with 2- to 5-mm-long leaflets, some success was also obtained with leaflets 8–13 mm long. However, no buds were regenerated when fully expanded leaflets were cultured.Development of buds into shoots was readily achieved by transferring regenerated buds into fresh medium containing 0.01 mg/L NAA and 1 mg/L BA. A few roots were induced to grow when callus with buds was also transferred to medium devoid of hormones. So far, bud regeneration from immature leaves has been induced in vitro in 5 of the 10 cultivars tested.

In Vitro Regeneration of Valencia-type Peanut (Arachis hypogaea L.) from Cultured Petiolules, Epicotyl Sections and Other Seedling Explants1

1992 ◽  
Vol 19 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Ming Cheng ◽  
David C. H. Hsi ◽  
Gregory C. Phillips
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Arachis Hypogaea L ◽  
Wide Range ◽  
Cultivated Peanut

Abstract This study evaluated plant development via direct organogenesis from in vitro-cultured young seedling tissues of cultivated peanut, especially the valencia-type peanut. Complete plants were regenerated from in vitro-cultured petiolule-with-blade-attached explants, leaflet segments, and epicotyl andpetiole sections. Multiple shoots arose on Murashige and Skoog medium (MS) supplemented with 6-benzylaminopurine (BA) (5–25 mg/L) plus 1-naphthaleneacetic acid (NAA) (0.5–3 mg/L). After 30 d culture on 25 mg/L BA + 1 mg/L NAA, 1.6 buds or shoots/explant were regenerated from the petiolule-with-blade-attached explants. Comparable numbers of shoots were obtained from epicotyl sections of the first node region of the seedling after 60 d culture using 10 mg/L BA + 1 mg/L NAA. Leaflet segments and petiole sections were less responsive for shoot formation. Excised shoots developed roots in vitro upon transfer for 15 d to MS medium supplemented with NAA at 1 mg/L. Plantlets were transferred to soil and grown in a greenhouse to maturity. A wide range of cultivated peanut genotypes was evaluated for organogenic responsiveness, using the petiolule-with-blade-attached explant source. Only valencia-type cultivars, or a hybrid derivative with a Valencia background, were responsive with this regeneration system.

In Vitro Culture of Immature Peanut (Arachis spp.) Leaves: Morphogenesis and Plantlet Regeneration1,2

1983 ◽  
Vol 10 (1) ◽  
pp. 21-25 ◽  
Author(s):  
R. N. Pittman ◽  
D. J. Banks ◽  
J. S. Kirby ◽  
E. D. Mitchell ◽  
P. E. Richardson
Keyword(s):  
In Vitro Culture ◽  
Histological Examination ◽  
Epidermal Cells ◽  
Naphthaleneacetic Acid ◽  
Genotypic Differences ◽  
Germinated Seeds

Abstract Green immature leaflets (2-5 mm in length from shoots of germinated seeds or greenhouse grown plants) from species representing seven taxonomic sections of the genus Arachis (Ambinervosae, Arachis, Caulorhizae, Erectoides, Extranervosae, Rhizomatosae, and Triseminalae), were cultured aseptically, in vitro, on a medium composed of Murashige and Skoog salts, Gamborg's B5 vitamins, 0.8% Difco agar, and supplemented with 1 mg/L each of naphthaleneacetic acid and N-6 benzyladenine. Histological examination of the cultures revealed that the meristematic areas originated from epidermal cells. Embryoids and meristematic shoots developed after lysis of the surrounding cells. All species of Arachis tested produced callus. Genotypic differences for the production of callus, shoots, and roots were observed with cultivated peanuts. Organogenesis occurred in the leaflet cultures, and plants were recovered from sections Arachis and Extranervosae of the genus Arachis.

Arachis hypogaea Plant Recovery Through in Vitro Culture of Peg Tips1

1995 ◽  
Vol 22 (2) ◽  
pp. 129-135 ◽  
Author(s):  
Q. L. Feng ◽  
H. T. Stalker ◽  
H. E. Pattee ◽  
T. G. Isleib
Keyword(s):  
In Vitro Culture ◽  
Embryo Development ◽  
Arachis Hypogaea ◽  
Reproductive Traits ◽  
Root Production ◽  
In Vitro Techniques ◽  
Arachis Hypogaea L ◽  
Vitro Development ◽  
Mature Seeds

Abstract In vitro culture of embryos in Arachis is necessary to recover interspecific hybrids which otherwise abort soon after fertilization. The objective of this research was to develop in vitro techniques to promote proembryo development so that plants can be recovered. Aerial peg tips consisting of embryos, ovules, and peg meristem of Arachis hypogaea L. cv. NC 6, were collected 7, 10, and 14 d after self-pollination. Peg tips were cultured in the dark on combined MS and B5 media with NAA, GA3 and 6-BAP for 90 d. The effects of plant growth regulators on in vitro reproductive traits, including peg elongation, callus and root production, pod formation, ovule and embryo development were variable. Results indicated that 10-d-old peg tips, which contained eight-celled proembryos, had more embryo development and pod formation than 7- and 14-d-old peg tips. Medium with 4 mg L-1 NAA and 0.5 mg L-1 6-BAP suppressed in vitro development of pods, ovules and embryos and induced large amounts of callus. Media with lower concentrations of NAA, GA3, and 6-BAP caused development of more and larger pods and ovules. The development of young embryos from proembryos was observed and mature seeds were obtained by an in vitro one-step process. Peanut plants were obtained both from in vitro-recovered embryos and from mature seeds.

In vitro regeneration of Arachis hypogaea L. and Moringa oleifera Lam. using extracellular phytohormones from Aphanothece sp. MBDU 515

2015 ◽  
Vol 7 ◽  
pp. 100-105 ◽  
Author(s):  
Manickam Gayathri ◽  
Pichaimuthu Senthil Kumar ◽  
Azhagiya Manavalan Lakshmi Prabha ◽  
Gangatharan Muralitharan
Keyword(s):  
Arachis Hypogaea ◽  
Moringa Oleifera ◽  
In Vitro Regeneration ◽  
Arachis Hypogaea L ◽  
Moringa Oleifera Lam
Sign in / Sign up

Export Citation Format

Share Document