Effect of auxins and cytokinins on efficient plant regeneration and multiple-shoot formation from cotyledons and cotyledonary-node expiants of groundnut (Arachis hypogaea L.) by in vitro culture technology

1997 ◽  
Vol 67 (3) ◽  
pp. 237-247 ◽  
Author(s):  
Perumal Venkatachalam ◽  
Narayanasamipillai Jayabalan
Keyword(s):  
Plant Regeneration ◽  
In Vitro Culture ◽  
Arachis Hypogaea ◽  
Cotyledonary Node ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Multiple Shoot Formation ◽  
Arachis Hypogaea L ◽  
Culture Technology

scholarly journals In Vitro Callus Culture and Plant Regeneration from Different Explants of Groundnut(Arachis hypogaea L.).

1996 ◽  
Vol 46 (4) ◽  
pp. 315-320
Author(s):  
Perumal Venkatachalam ◽  
Adaikalam Subramaniampillai ◽  
Narayanasamylpillai Jayabalan
Keyword(s):  
Plant Regeneration ◽  
Arachis Hypogaea ◽  
Callus Culture ◽  
Arachis Hypogaea L

Regeneration of peanut (Arachis hypogaea) plantlets by in vitro culture of immature leaves

1981 ◽  
Vol 59 (5) ◽  
pp. 826-830 ◽  
Author(s):  
L. A. Mroginski ◽  
K. K. Kartha ◽  
J. P. Shyluk
Keyword(s):  
In Vitro Culture ◽  
Arachis Hypogaea ◽  
Environmental Conditions ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Bud Regeneration ◽  
Arachis Hypogaea L ◽  
Murashige And Skoog Medium ◽  
Germinated Seeds

The in vitro regeneration of buds, shoots, and roots from immature leaves of 3- to 5-day-old peanut (Arachis hypogaea L. cv. Colorado Manfredi) seedlings was studied under defined nutritional, hormonal, and environmental conditions. The first two leaves (2–5 mm in length) removed from aseptically germinated seeds were cultured on Murashige and Skoog medium containing vitamins as in B5 medium and 0.8% agar, supplemented with 12 combinations of naphthaleneacetic acid (NAA) (0.01 to 4 mg/L) and benzyladenine (BA) (1 and 3 mg/L). Bud regeneration occurred in all hormone combinations, but the maximum number of buds was regenerated at a concentration of 1 mg/L each of NAA and BA. Although bud regeneration was maximum with 2- to 5-mm-long leaflets, some success was also obtained with leaflets 8–13 mm long. However, no buds were regenerated when fully expanded leaflets were cultured.Development of buds into shoots was readily achieved by transferring regenerated buds into fresh medium containing 0.01 mg/L NAA and 1 mg/L BA. A few roots were induced to grow when callus with buds was also transferred to medium devoid of hormones. So far, bud regeneration from immature leaves has been induced in vitro in 5 of the 10 cultivars tested.

scholarly journals Regeneration and In Vitro Flowering in Brassica Campestris (L.) Var. Bhavani

Our Nature ◽  
1970 ◽  
Vol 5 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Rinki Verma ◽  
RR Singh
Keyword(s):  
Shoot Apex ◽  
Brassica Campestris ◽  
Cotyledonary Node ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
In Vitro Flowering ◽  
Cotyledonary Nodes ◽  
Improvement Programs ◽  
Shoot Apex Explants

Multiple shoot formation and in vitro flowering was found in Brassica campestris (L.) var. Bhavani. Maximum numbers of shoots were produced in both cotyledonary node and shoot apex explants on MS-media supplemented with BA (2.5 mg/l) + IAA (1.0 mg/l) + Kn (0.5 mg/l). Maximum flowering (50%) was noted at IBA (1.5 mg/l) + IAA (1.0 mg/l) + Kn (0.5 mg/l) in the shoots from cotyledonary nodes. In vitro flowering may contribute in many ways to Brassica Improvement Programs. The shoots rooted well in the half and full strength media each with IBA (1.0 mg/l) and NAA (1.0 mg/l) and the plantlets have been maintained. Keywords: Brassica campestris, In vitro flowering, Regeneration.doi:10.3126/on.v5i1.793Our Nature (2007)5:21-24

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