Intraseasonal variation in chlorophyll content and chloroplast ultrastructure of selected plant species in a deciduous forest

1980 ◽  
Vol 58 (13) ◽  
pp. 1504-1519 ◽  
Author(s):  
B. W. Floyd ◽  
R. D. Noble
Keyword(s):  
Chlorophyll Content ◽  
Deciduous Forest ◽  
Starch Content ◽  
Acer Rubrum ◽  
Chloroplast Ultrastructure ◽  
Quercus Alba ◽  
Canopy Closure ◽  
Chlorophyll B ◽  
Intraseasonal Variation ◽  
Starch Grains

Chlorophyll content and chlorophyll a – chlorophyll b ratios were determined at regular intervals over a 3-month period for eight forest species selected from different levels in a deciduous forest. Chloroplast ultrastructure and starch content were investigated in five of the eight species, chosen to be representative of each level.Chlorophyll a – chlorophyll b ratios for Quercus alba L., Liriodendron tulipifera L., Acer rubrum L., Cornus florida L., Desmodium nudiflorum (L.) DC., and Sassafras albidum (Nuttall) Nees reached a peak at the time of canopy closure whereas total chlorophyll content reached a maximum 1–3 weeks later. The number of thylakoids per granum in all species increased after canopy closure. Chlorophyll content and variations in thylakoid number per granum were found to be related to light intensity variations.Starch content, based on size and number of starch grains, remained stable prior to and after closure in Q. alba, D. nudiflorum, and S. albidum, where a decrease in the number of starch grains was observed after closure. The number of starch grains in D. nudiflorum appeared to decrease during the period 6 weeks after closure. The variations in starch grain number were an indication of photosynthetic activity in these species.The disorganization of grana thylakoids and the development of electron dense and transparent bodies in the chloroplast stroma of all species sampled, 6 weeks after closure, was a preliminary indication of leaf senescence. Electron transparent bodies were considered to be by-products of grana thylakoid degradation.

scholarly journals Genetic analysis of chlorophyll content in maize by mixed major and polygene models

Genetika ◽  
2014 ◽  
Vol 46 (3) ◽  
pp. 1037-1046 ◽  
Author(s):  
Muhammad Irfan ◽  
Jia-Xing Sun ◽  
Yanbin Liu ◽  
Xue Li ◽  
Shuang Yang
Keyword(s):  
Genetic Analysis ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Segregation Analysis ◽  
Major Gene ◽  
Chlorophyll B ◽  
Major Genes ◽  
Main Gene ◽  
Joint Segregation ◽  
Joint Segregation Analysis

Chlorophyll is an important factor which also affects the yield in maize. In this study, genetic analysis of chlorophyll content was conducted by joint segregation analysis of four generations P1, P2, F1 and F2:4 from the cross Shen3336?Shen3265 using the mixed major genes and polygenes inheritance models. Genetics of chlorophyll revealed that chlorophyll ?a? was controlled by two main gene having additive-dominanceepistasis effects. The heritability of these genes were 56.3%. Chlorophyll ?b? was controlled by two pairs of codominant major gene plus additive-dominance polygene. The heritability of these major genes and polygenes were 1.12% and 93.26% respectively. Chlorophyll ?a+b? was controlled by two pairs of additive-dominance- epistatic major genes plus additive-dominance polygene having heritability of 56.2% and 5.2% respectively.

scholarly journals Analisis Kandungan Klorofil Pada Anak Daun Tanaman Kelapa (Analysis of Chlorophyll Content in Children Leaves of Coconut Plants)

2019 ◽  
Vol 9 (2) ◽  
pp. 76
Author(s):  
Muhammad Tawary ◽  
Julius Pontoh ◽  
Lydia Momuat
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Economic Value ◽  
Economic Benefits ◽  
Chlorophyll B ◽  
Solvent Extraction Method ◽  
Chlorophyll A And B ◽  
The Right ◽  
Almost All ◽  
Chlorophyll A Content

Analisis Kandungan Klorofil Pada Anak Daun Tanaman Kelapa (Analysis of Chlorophyll Content in Children Leaves of Coconut Plants) Muhammad Tawary1*), Julius Pontoh1), Lydia I.Momuat1)1)Jurusan Kimia, FMIPA UNSRAT Manado*Email korespondensi: [email protected] Diterima 7 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kelapa banyak dibudidayakan sebagai tanaman pertanian. Tanaman kelapa memiliki nilai ekonomi yang tinggi karena hampir semua bagian tanaman kelapa memiliki manfaat ekonomis. Penelitian bertujuan untuk  mengembangkan metode analisis klorofil pada daun dan menentukan bagian anak daun yang memiliki kandungan klorofil tertinggi. Analisis kandungan klorofil dilakukan dengan metode ektraksi pelarut yang absorbansinya dibaca pada spektrofotometer UV-Vis. Kandungan klorofil a dalam daun bervariasi pada setiap bagian anak daun tanaman kelapa. Kandungan klorofil a pada posisi tengah anak daun kelapa (763.19 µmol/m2) dan kandungan klorofil b (196.22 µmol/m2). Kandungan klorofil a dan b pada setiap bagian anak daun dan posisi kanan dan kiri anak daun memiliki nilai yang relatif sama.Kata kunci: Kelapa, klorofil a, klorofil b Abstract Many coconut plants are cultivated as agricultural crops. Coconut plants have high economic value because almost all parts of coconut plants have economic benefits. The study aims to develop a method of chlorophyll analysis on leaves and determine the part of leaflets which have the highest chlorophyll content. Analysis of chlorophyll content was carried out by a solvent extraction method in which the absorbance was read in a UV-Vis spectrophotometer. The content of chlorophyll a in leaves varies with each part of the leaves of the coconut plant. Chlorophyll a content in the middle position of coconut leaf child (763.19 µmol / m2) and chlorophyll b content (196.22 µmol / m2). The content of chlorophyll a and b in each part of the leaf child and the right and left positions of the leaves have relatively the same value.Keywords: Coconut, Chlorophyll a, Chlorophyll b

scholarly journals Antioxidant gene expression, chlorophyll, and starch content in Persian lime (Citrus latifolia Tanaka Ex Q. Jiménez) trees with HLB by application of elicitors of plant resistance

2021 ◽  
Vol 6 (2) ◽  
pp. 86-102
Author(s):  
Felipe Roberto Flores-de la Rosa ◽  
◽  
Ricardo Santillán-Mendoza ◽  
Cynthia Guadalupe Rodríguez-Quibrera ◽  
Antonio Martínez-Ruiz ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Starch Content ◽  
Starch Accumulation ◽  
Economic Losses ◽  
Total Chlorophyll ◽  
Gamma Aminobutyric Acid ◽  
Antioxidant Genes ◽  
Spectrophotometric Methods ◽  
Total Chlorophyll Content ◽  
Randomized Design

Persian lime is economically important for Mexico. However, the disease known as Huanglongbing (HLB) causes chlorosis symptoms in the foliage that affect the growth and decreases the yield of the trees. The expression of chlorosis symptoms is associated with starch accumulation and inhibition of antioxidant activity. The objective of the present work was to determine the effect of the application of three resistance elicitors on the expression of three antioxidant genes and on starch and chlorophyll content in Persian lime trees with HLB. Salicylic acid (SA), jasmonic acid (JA) or gamma-aminobutyric acid (GABA) were applied every eight days for nine weeks using a completely randomized design and a one-way ANOVA was performed for the analysis. Expression of APX, CAT and SOD genes was quantified at week nine at four different times. Starch and total chlorophyll content was estimated every three weeks by spectrophotometric methods. The application of the elicitors significantly increased the expression of the three genes, with SA and GABA generating the greatest increase at different times of application. No difference was found in starch and total chlorophyll content at most of the times evaluated. The use of resistance elicitors is promising in the management of HLB in plots already infected, seeking to extend the productive life of the orchards and thus counteract the economic losses caused by HLB.

scholarly journals Variations of Chlorophyll Content at Abies alba and Nepeta pannonica Species According to Phenophase and Harvesting Area

2019 ◽  
Vol 52 (1) ◽  
pp. 74-78
Author(s):  
S. Buhăianu ◽  
Doina Carmen Jităreanu
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Photosynthetic Activity ◽  
Abies Alba ◽  
Chlorophyll B ◽  
Green Color ◽  
Yellow Coloration ◽  
Chlorophyll A And B ◽  
Chlorophyll A Content

Abstract Chlorophylls from plants are photosynthetic pigments. Their quantity offers valuable informations about photosynthetic activity, growing and developing of plants. Photosynthetic pigments decrease quantitatively during senescence process or in stress conditions. The present study has been realized in laboratory conditions with material harvested from spontaneous flora. The purpose of this research was the investigation of variations of chlorophyll content from samples of biological material collected from Nepeta pannonica L. and Abies alba Mill. plants, from Câmpulung Moldovenesc and Cacica areas, Suceava county, Romania. The targeted phenophases were growth and flowering. There were realized acetonic extracts from samples for spectrophotometric determinations. Obtained data were processed to estabilish chlorophyll a and b content. There were observed that at Abies alba species, from both locations, the chlorophyll a content grew during flowering phenophase, while the chlorophyll b content had little variations. At Nepeta pannonica species, the chlorophyll a and b content decreased visibly during the flowering, due to stress. Leaves of plants from this species presented a intense green color in the growing phenophase, while during flowering phenophase they had a purple or yellow coloration. Obtained results revealed a different dynamics of chlorophyll content at studied species.

scholarly journals Dynamics of Polysaccharides and Neutral Lipids during Anther Development in Castor (Ricinus communis)

2015 ◽  
Vol 140 (4) ◽  
pp. 356-361 ◽  
Author(s):  
Dongmei Wei ◽  
Huimin Xu ◽  
Ruili Li
Keyword(s):  
Neutral Lipids ◽  
Ricinus Communis ◽  
Starch Content ◽  
Single Layer ◽  
Pollen Grains ◽  
Middle Layer ◽  
Anther Development ◽  
Anther Wall ◽  
Starch Grains ◽  
Tapetal Cells

Anthers contain starch and neutral lipids, which have key roles in microspore ontogeny and gametophyte development. In this study, we observed the dynamic changes in starch and neutral lipids in the anther developmental processes of castor (Ricinus communis) by cytochemical methods. Starch grains and neutral lipids presented a regular dynamic distribution during anther development. In young anthers, some neutral lipids accumulated in sporogenous cells, whereas neutral lipids disappeared with microspore growth. At the late microspore stage, starch grains began to accumulate in microspores, and the starch content of bicellular pollen significantly increased after microspore mitosis. At anthesis, starch grains and neutral lipids accumulated in the mature pollen grains. Visible changes occurred in anther wall cells. The epidermis, middle layer, and tapetum were degenerated, and only a single layer of endothecium remained at anthesis. The dynamic variation of starch grains and neutral lipids in tapetal cells was consistent with the changes in microspores and pollen during anther development. All these findings demonstrated that tapetal cells directly interacted with the developing gametophytes. The tapetal cells play an important role in supplying nutritional substances for microspore absorption. Moreover, the endothecium protects the pollen and contributes to anther dehiscence. The results of this study provide a foundation for the further research on sexual reproduction in angiosperms.

scholarly journals Pemanfaatan Pupuk Organik Kulit Pisang Dan Azolla Terhadap Pertumbuhan Kangkung Darat Dengan Sistem Akuaponik

2019 ◽  
Vol 12 (01) ◽  
pp. 1-10
Author(s):  
Maria Sastriana ◽  
Ngadiani Ngadiani
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Organic Fertilizer ◽  
Control Treatment ◽  
Banana Peel ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Azolla Pinnata ◽  
Randomized Design ◽  
Weight Test

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.).   Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.

scholarly journals Brassinosteroids Regulate Antioxidant System and Protect Chloroplast Ultrastructure of Autotoxicity-Stressed Cucumber (Cucumis sativus L.) Seedlings

Agronomy ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 265 ◽  
Author(s):  
Ping Yang ◽  
Muhammad Azher Nawaz ◽  
Fuxin Li ◽  
Lisha Bai ◽  
Jie Li
Keyword(s):  
Net Photosynthetic Rate ◽  
Chlorophyll Content ◽  
Photosynthetic Rate ◽  
Soluble Sugar ◽  
Chloroplast Ultrastructure ◽  
Dry Weight ◽  
Antioxidant Defense System ◽  
Stress Conditions ◽  
Vegetable Crops ◽  
Cucumis Sativus L

Autotoxicity is a common problem being faced in protected vegetable cultivation system. Phytoremediation of plant autotoxicity is an emerging concept to minimize deterioration of soil environment and reduction of yield and quality of vegetable crops. Brassinosteroids (BRs) have been reported as a potential phytohormone to assist phytoremediation. However, the effects of BRs-induced autotoxicity stress on plant growth, photosynthesis and antioxidant defense system are poorly understood. Hence, we focused on the changes in physiological characteristics and ultrastructure of cucumber leaves in response to the application of 24-epibrassinolide (EBR) under autotoxicity stress conditions. The results showed that leaf area, plant height, fresh weight and dry weight of cucumber were obviously decreased under autotoxicity stress conditions. EBR application obviously improved the phenotypic characteristics of cucumber seedlings. Chlorophyll content, net photosynthetic rate, stomatal conductance and transpiration rate of cucumber leaves were markedly reduced under autotoxicity stress conditions. Application of EBR improved the photosynthetic pigments (chlorophyll a by 15.80%, chlorophyll b by 18.70% and total chlorophyll content by 17.30%), net photosynthetic rate by 36.40% and stomatal opening of leaves under autotoxicity stress conditions. EBR application also maintained the integrity of chloroplast and thylakoid structures under autotoxicity stress conditions. The activity of catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX) and antioxidative compounds ascorbate (AsA) and reduced glutathione (GSH) contents were markedly decreased, however, these were obviously increased after EBR application under autotoxicity stress. EBR application also increased the soluble sugar and protein, and proline concentration by 59.70%, 7.22% and 36.58%, respectively in the leaves of cucumber, decreased malondialdehyde by 24.13% and reactive oxygen species contents (H2O2 by 35.17%, O2− by 12.01% and •OH by 16.59%), and reduced the relative permeability of the cell membrane by 14.31%. These findings suggest that EBR application enhanced the photosynthetic capacity of leaves, maintained the integrity of chloroplast and thylakoid structures, and effectively alleviated the damage of membrane caused by lipid peroxidation and root damage under autotoxicity stress conditions. The growth inhibition effect of autotoxicity stress on cucumber was reduced by EBR application.

Hypholoma lateritiumisolated from coarse woody debris, the forest floor, and mineral soil in a deciduous forest in New Hampshire

Botany ◽  
2012 ◽  
Vol 90 (6) ◽  
pp. 457-464 ◽  
Author(s):  
Therese A. Thompson ◽  
R. Greg Thorn ◽  
Kevin T. Smith
Keyword(s):  
New Hampshire ◽  
Deciduous Forest ◽  
Repetitive Sequences ◽  
Mineral Soil ◽  
Acer Rubrum ◽  
Organic Soil ◽  
Nuclear Ribosomal Dna ◽  
Soil Horizon ◽  
Soil Horizons ◽  
Internal Transcribed Spacer Region

Fungi in the Agaricomycetes (Basidiomycota) are the primary decomposers in temperate forests of dead wood on and in the forest soil. Through the use of isolation techniques selective for saprotrophic Agaricomycetes, a variety of wood decay fungi were isolated from a northern hardwood stand in the Bartlett Experimental Forest, New Hampshire, USA. In particular, Hypholoma lateritium (Schaeff.: Fr.) P. Kumm. was isolated from basidiocarps, decaying Acer rubrum L. logs, the Oe organic soil horizon, and the E and BC mineral soil horizons. Identification was confirmed by sequence analysis of the internal transcribed spacer region of nuclear ribosomal DNA. All isolates had identical sequences in this region to previously published sequences for the species; some were monokaryotic and simple-septate and others were dikaryotic, with clamp connections. Isolates were further characterized by banding patterns (DNA fingerprints) produced with PCR primers based in simple repetitive sequences and the minisatellite M13. Nine dikaryotic isolates from basidiocarps and from soil horizons Oe, E, and BC had identical fingerprint patterns with all primers tested. The confirmed presence of H. lateritium suggests that this fungus could form a mycelial translocation network that bridges mineral and organic soil horizons and decaying logs.

scholarly journals Mapping Canopy Chlorophyll Content in a Temperate Forest Using Airborne Hyperspectral Data

2020 ◽  
Vol 12 (21) ◽  
pp. 3573
Author(s):  
J. Malin Hoeppner ◽  
Andrew K. Skidmore ◽  
Roshanak Darvishzadeh ◽  
Marco Heurich ◽  
Hsing-Chung Chang ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Spectral Resolution ◽  
Near Infrared ◽  
Deciduous Forest ◽  
Mixed Forest ◽  
Hyperspectral Data ◽  
Least Squares Regression ◽  
Area Index ◽  
Management Interventions ◽  
Shortwave Infrared

Chlorophyll content, as the primary pigment driving photosynthesis, is directly affected by many natural and anthropogenic disturbances and stressors. Accurate and timely estimation of canopy chlorophyll content (CCC) is essential for effective ecosystem monitoring to allow for successful management interventions to occur. Hyperspectral remote sensing offers the possibility to accurately estimate and map canopy chlorophyll content. In the past, research has predominantly focused on the use of hyperspectral data on canopy chlorophyll content retrieval of crops and grassland ecosystems. Therefore, in this study, a temperate mixed forest, the Bavarian Forest National Park in Germany, was chosen as the study site. We compared different statistical models (narrowband vegetation indices (VIs), partial least squares regression (PLSR) and random forest (RF)) in their accuracy to predict CCC using airborne hyperspectral data. The airborne hyperspectral imagery was acquired by the AisaFenix sensor (623 bands; 3.5 nm spectral resolution in the visible near-infrared (VNIR) region, and 12 nm spectral resolution in the shortwave infrared (SWIR) region; 3 m spatial resolution) on July 6, 2017. In situ leaf chlorophyll content and leaf area index measurements were sampled from the upper canopy of coniferous, mixed, and deciduous forest stands in July and August 2017. The study yielded the highest retrieval accuracies with PLSR (root mean square error (RMSE) = 0.25 g/m2, R2 = 0.66). It further indicated specific spectral regions within the visible (390–400 nm and 470–540 nm), red edge (680–780 nm), near-infrared (1050–1100 nm) and shortwave infrared regions (2000–2270 nm) that were important for CCC retrieval. The results showed that forest CCC can be mapped with relatively high accuracies using image spectroscopy.

Effect of plant growth regulators on leaf area, chlorophyll content, carotenoids, stomatal count and yield of cashew (Anacardium occidentale L.) var. Bhaskara

2017 ◽  
Vol 45 (2) ◽  
pp. 141
Author(s):  
Lakshmipathi, J. D. Adiga D. Kalaivanan and G.K. Halesh
Keyword(s):  
Plant Growth ◽  
Leaf Area ◽  
Chlorophyll Content ◽  
Growth Regulators ◽  
Biochemical Parameters ◽  
Foliar Application ◽  
Growth Stages ◽  
Chlorophyll B ◽  
Nut Production ◽  
Stomatal Number

<p>An experiment was conducted to study the effect of exogenous application of growth regulators at three important growth stages<br />(flushing, flowering and fruiting) on leaf area, chlorophyll content, carotenoids, stomatal count and yield of cashew var. Bhaskara.<br />Irrespective of growth stages, foliar application of GA3 @ 50 ppm and ethrel @ 50 ppm was found to be superior in all the<br />parameters and on par with each other compared to other growth regulators. Out of nine treatments of different growth regulators;<br />the highest leaf area was recorded in trees sprayed with GA3 @ 50 ppm and ethrel @ 50 ppm. At flushing stage, spraying with GA3<br />@ 50 ppm resulted in highest stomatal number (21.9) and carotenoids (0.41) whereas unsprayed (control) trees recorded least<br />stomatal number (11.6) and carotenoids (0.19). Thus, leaf area, chlorophyll content, carotenoids and stomatal count increased in<br />trees sprayed with growth regulators than unsprayed trees. Spraying of ethrel @ 50 ppm recorded highest nut yield<br />(14.3 kg tree-1) followed by NAA @ 25 ppm + GA3 50 ppm (12.9 kg tree-1). This study demonstrated the potential of ethrel as well<br />as GA3 in improving various biochemical parameters viz., chlorophyll ‘a’, chlorophyll ‘b’, carotenoids and leaf area in cashew<br />which are important determinants in increasing nut production.</p>

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