An interrelationship among peroxidase, IAA oxidase, and polyphenol oxidase from peanut cells

1977 ◽  
Vol 55 (20) ◽  
pp. 2630-2635 ◽  
Author(s):  
O. P. Srivastava ◽  
R. B. van Huystee
Keyword(s):  
Acetic Acid ◽  
Polyphenol Oxidase ◽  
Peroxidase Activity ◽  
Active Site ◽  
Indole Acetic Acid ◽  
Phenol Oxidase ◽  
Iaa Oxidase

Two methods were used to separate heme and apoenzyme from the holoenzyme of peroxidase (EC 1.11.1.7) isolated from medium of cultured peanut cells. Apoenzyme prepared by either method lacked peroxidase activity but possessed indole acetic acid (IAA) oxidase and poly phenol oxidase (PPO) (EC 1.14.18.1) activity. When the holoenzyme was reconstituted with heme and apoenzyme, peroxidase activity was restored. The studies on the active site revealed that PPO and IAA oxidase share the same active site on the apoenzyme.

The heme moiety in peanut peroxidase

1984 ◽  
Vol 62 (11) ◽  
pp. 1046-1050 ◽  
Author(s):  
R. N. Chibbar ◽  
R. Cella ◽  
R. B. Van Huystee
Keyword(s):  
Peroxidase Activity ◽  
Active Site ◽  
Oxidase Activity ◽  
Indoleacetic Acid ◽  
Iaa Oxidase ◽  
Equimolar Ratio ◽  
Immunological Properties ◽  
Peanut Peroxidase

Heme is present in an equimolar ratio to the apoprotein in the major cationic fraction of peanut peroxidase. The removal of heme from the holoenzyme does not affect the physicochemical and immunological properties of the apoperoxidase, however peroxidase activity is completely lost. The indoleacetic acid (IAA) oxidase activity of the apoperoxidase is reduced to 1/20 of the original holoenzyme. Both the peroxidase and IAA-oxidase activity could partially be restored in the holoenzyme reconstituted with hemin. It is suggested that heme may also participate in the IAA-oxidase activity possibly by altering the active site.

scholarly journals Activity of indolyl-3-acetic acid oxidase and peroxidase in roots of carrot infested with Meloidogyne hapla Chiuu.

2015 ◽  
Vol 29 (1) ◽  
pp. 107-117
Author(s):  
Krystyna M. Janas
Keyword(s):  
Acetic Acid ◽  
Peroxidase Activity ◽  
Acid Oxidase ◽  
Healthy Controls ◽  
Meloidogyne Hapla ◽  
Secondary Phloem ◽  
Iaa Oxidase ◽  
Kinetics Of

IAA-oxidase and peroxidase activity was measured in storage and side roots of healthy and <i>M. hapla</i> infested carrots of two sultivars. Cultivar 'Perfekcja' is sensitive whereas cv. 'Slendero' is tolerant to the northern root-knot ne-matode. 3-, 4-, and 5-month-old plants were subjected to analyses. In <i>M. hapla</i> infested plants of both cultivars IAA-oxidase inhibitors accumulated. Kinetics of IAA oxidation <i>in vivo</i> were the same in healthy and infested plants. IAA-oxidase activity <i>in vitro</i> was inhibited in crude extracts of the infested tissues, the inhibition being prevented by PVP. Peroxidase activity increased in secondary phloem and decreased in galled side roots of both cultivars when compared with healthy controls. In galled side roots of the youngest 3-month-old plants peroxidase activity was not decreased. IAA-oxidase inhibitors accumulated in the infested roots.It is concluded that <i>M. hapla</i> has no direct effect on IAA-oxidase. Degree of tolerance to nematodes is correlated with the ratio of IAA-oxidase inhibitors to IAA-oxidase rather than with the absolute activity of IAA-oxidase.

Evidence for close association of peroxidase, polyphenol oxidase, and IAA oxidase isozymes of peanut suspension culture medium

1973 ◽  
Vol 51 (11) ◽  
pp. 2207-2215 ◽  
Author(s):  
O. P. Srivastava ◽  
R. B. van Huystee
Keyword(s):  
Acetic Acid ◽  
Suspension Culture ◽  
Polyphenol Oxidase ◽  
Close Association ◽  
Exchange Chromatography ◽  
Acid Extraction ◽  
Iaa Oxidase ◽  
Elution Pattern ◽  
Acetic Acid Extraction ◽  
Acetone Fractionation

Peroxidase, polyphenol oxidase, and indole-3-acetic acid (IAA) oxidase were detected in a 7-day-old medium of a peanut cotyledon cell suspension culture. Preliminary separation of the isozymes of these three enzymes was achieved by acetone fractionation and acetic acid extraction. By means of zymograms on polyacrylamide gels, five anionic and two cationic peroxidase isozymes were identified. Staining of the gels for polyphenol oxidase and IAA oxidase revealed an Rf pattern for a number of isozymes that was identical with that found for peroxidase. These isozymes were separated using ion-exchange chromatography. Again the elution pattern for the isozymes of the three enzymes was so similar that it can be regarded as an enzyme possessing all the three types of activity.

scholarly journals Impact of some phytohormones, metabolic inhibitor and agrochemicals on senescing paddy leaves and its relevance to productivity

2017 ◽  
Vol 6 (05) ◽  
pp. 1606
Author(s):  
Haseena Rafath
Keyword(s):  
Acetic Acid ◽  
Peroxidase Activity ◽  
Indole Acetic Acid ◽  
Oryza Sativa L ◽  
Metabolic Inhibitors ◽  
Metabolic Inhibitor ◽  
Exact Nature ◽  
Experimental Conditions ◽  
Dichlorophenoxy Acetic Acid ◽  
Made In

The influence of hormones viz. Indole acetic acid (IAA), Gibberellic acid (GA3), Cytokinins viz. Benzyladenine (BA), Benzimidazole (BZI), biocides viz. 2,4-Dichlorophenoxy acetic acid (2,4-D), Diathane M-45, Dimecron and metabolic inhibitors viz. Cycloheximide (CHI) and Actinomycin-D (Act-D) on senescence in detached paddy (Oryza sativa L.) leaves was studied under dark and illuminated conditions. Cytokinins were found to be more effective senescence retardants in light than in dark while auxins were effective in light. Biocides proved to be milder senescence retardants. Effective concentration, a concentration away from the ineffective and toxic range, chosen from IAA, GA3, BA, BZI, 2,4-D, Diathane M-45 and Dimecron were 25,100, 5, 100, 25, 1500 and 0.50 ppm respectively. Application of Act-D (inhibitor of mRNA synthesis) with in 15 min after detachment and cycloheximide (inhibitor of cytoplasmic protein synthesis) from the start of the incubation resulted in suppression of peroxidase activity both under light and dark. Catalase activity exhibited an inverse relation with peroxidase activity under both the experimental conditions. It is recommended that attempts must be made in a more sophisticated way to study the changes in activities of different enzymes suspected to be linked with leaf senescence at a deeper level using all modern techniques available so that the exact nature of enzymes, the isoenzymes pattern and their relation with yield can be understood. Such studies may also help in future in manipulating the projects for the improvement of this economically important cereal crop.

Peroxidase Activity May Play a Role in the Cytotoxic Effect of Indole Acetic Acid*

1997 ◽  
Vol 65 (2) ◽  
pp. 338-341 ◽  
Author(s):  
Mariza Pires de Melo ◽  
Tania Cristina Pithon Curi ◽  
Rui Curl ◽  
Paolo Di Mascio ◽  
Giuseppe Cilento
Keyword(s):  
Acetic Acid ◽  
Peroxidase Activity ◽  
Cytotoxic Effect ◽  
Indole Acetic Acid

Isolation and identification of IAA producing endosymbiotic bacteria from Gracillaria corticata (J. Agardh)

2016 ◽  
Vol 5 (12) ◽  
pp. 5179
Author(s):  
Ilahi Shaik* ◽  
P. Janakiram ◽  
Sujatha L. ◽  
Sushma Chandra
Keyword(s):  
Acetic Acid ◽  
Culture Filtrate ◽  
Indole Acetic Acid ◽  
Shoot Growth ◽  
High Salinity ◽  
Saline Soils ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Endosymbiotic Bacteria ◽  
Root And Shoot Growth

Indole acetic acid is a natural phytohormone which influence the root and shoot growth of the plants. Six (GM1-GM6) endosymbiotic bacteria are isolated from Gracilaria corticata and screened for the production of IAA out of six, three bacterial strains GM3, GM5 and GM6 produced significant amount of IAA 102.4 µg/ml 89.40 µg/ml 109.43 µg/ml respectively. Presence of IAA in culture filtrate of the above strains is further analyzed and confirmed by TLC. As these bacterial strains, able to tolerate the high salinity these can be effectively used as PGR to increase the crop yield in saline soils.

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