Evidence for close association of peroxidase, polyphenol oxidase, and IAA oxidase isozymes of peanut suspension culture medium

1973 ◽  
Vol 51 (11) ◽  
pp. 2207-2215 ◽  
Author(s):  
O. P. Srivastava ◽  
R. B. van Huystee

Peroxidase, polyphenol oxidase, and indole-3-acetic acid (IAA) oxidase were detected in a 7-day-old medium of a peanut cotyledon cell suspension culture. Preliminary separation of the isozymes of these three enzymes was achieved by acetone fractionation and acetic acid extraction. By means of zymograms on polyacrylamide gels, five anionic and two cationic peroxidase isozymes were identified. Staining of the gels for polyphenol oxidase and IAA oxidase revealed an Rf pattern for a number of isozymes that was identical with that found for peroxidase. These isozymes were separated using ion-exchange chromatography. Again the elution pattern for the isozymes of the three enzymes was so similar that it can be regarded as an enzyme possessing all the three types of activity.

2020 ◽  
Vol 20 ◽  
pp. 101083
Author(s):  
Jamshed Ali Khan ◽  
Luong N. Nguyen ◽  
Hung C. Duong ◽  
Long D. Nghiem

1949 ◽  
Vol 27b (6) ◽  
pp. 572-579 ◽  
Author(s):  
Alan Bell ◽  
George F Wright

Acetone has been isolated by permanganate oxidation of birch and spruce lignins extracted from wood by acetic or formic acid. Acetone was obtained also when the formic acid lignins were ozonized but not from the acetic acid – extracted lignins. This difference was confirmed when perbenzoic acid titration indicated unsaturation in the formic acid extract which was not present in the acetic acid extract. It is suggested that an α-hydroxy-β-methoxyisobutyl group is present in lignin; during acetic acid extraction the hydroxyl group is acetylated but during formic acid extraction the formic acid reduces the group to isobutenyl, which will give acetone when it is ozonized.


1983 ◽  
Vol 18 (5) ◽  
pp. 421-440 ◽  
Author(s):  
Yue Kuo ◽  
Harry P. Gregor

1979 ◽  
Vol 93 (2) ◽  
pp. 509-511 ◽  
Author(s):  
R. G. McLaren ◽  
D. Purves ◽  
E. Jean Mackenzie ◽  
C. G. Mackenzie

There are extensive areas in Scotland and other parts of Britain where, because of low soil cobalt status, the cobalt concentration of pasture herbage is below the 0–1 /fg/g D.M. regarded as the requirement for grazing ruminants (Agricultural Research Council, 1965). A common method of overcoming the effects of cobalt deficiency in livestock occurring within these areas is to apply cobalt sulphate to the pasture. Following work carried out by Stewart, Mitchell & Stewart (1941, 1942) and Stewart etal.(1946) the standard recommended application of cobalt sulphate for pasture has remained at 2 kg/ha for many years, an application generally expected to last for 3–5 years. However, in South East Scotland there are doubts about the adequacy of such treatment which has led to a reappraisal. Significant increases in uptake of cobalt by herbage following applications to land at this rate are not always observed and sometimes there is no appreciable increase in ‘available’ soil cobalt as determined by acetic acid extraction.


2011 ◽  
Vol 98 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Tatiane de A. Maranhão ◽  
Jessee S.A. Silva ◽  
Vera L.A.F. Bascuñan ◽  
Fernando J.S. Oliveira ◽  
Adilson J. Curtius

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