Ultrastructure and development of phenolic-storing cells in cotton roots

1976 ◽  
Vol 54 (17) ◽  
pp. 2074-2082 ◽  
Author(s):  
W. C. Mueller ◽  
C. H. Beckman
Keyword(s):  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Electron Micrographs ◽  
Root Tip ◽  
Fixation Procedure ◽  
Rate Of Development ◽  
Temperature Development ◽  
Endodermal Cells ◽  
Surrounding Parenchyma

The development of phenolics in the radicle of cotton was determined with the nitroso reaction and light microscopy. In all cotton cultivars tested, phenolics could be detected in the endodermis at a distance of 1.0 to 2.0 mm from the root tip and in the root cap 0.5 mm from the root tip. By 3.0 mm the phenolic-storing cells in the endodermis were mature. The rate of development of the phenolic-storing endodermal cells was similar in all cultivars but varied with temperature, with maximum rates at 35 °C. At this temperature, development and maturation of the phenolic-storing cells occurred in 30 min. Phenolics could first be detected with the electron microscope in the vacuoles of endodermal cells 1.0 mm from the root tip; these cells were essentially mature 3.0 mm from the root tip. Except for the phenolics in the vacuoles, developing phenolic-storing cells were identical with surrounding parenchyma and contained abundant endoplasmic reticulum and ribosomes, mitochondria, dictyosomes, and large, variable plastids. Although several fixation methods were used and the appearance of the cells varied according to the fixation procedure, no positive origin for the phenolic material could be detected in the electron micrographs.

scholarly journals AN ELECTRON MICROSCOPE STUDY OF THE ENDOPLASMIC RETICULUM IN NEWT NOTOCHORD CELLS AFTER DISTURBANCE WITH ULTRASONIC TREATMENT AND SUBSEQUENT REGENERATION

1964 ◽  
Vol 20 (1) ◽  
pp. 175-183 ◽  
Author(s):  
G. G. Selman ◽  
A. Jurand
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Ultrasonic Treatment ◽  
Electron Microscope Study ◽  
Triturus Alpestris ◽  
Notochord Cells ◽  
Parallel Arrays ◽  
Subsequent Regeneration

Ultrasonic treatment of the tails of Triturus alpestris tadpoles, at intensities of 8 to 15 watts/cm2, at 1 megacycle/sec., for 5 minutes, disrupted the epidermis and caused pycnosis in individual cells of the muscle and neural tube, but caused no damage to the notochord that could be detected by light microscopy. Electron microscopy showed that this ultrasonic treatment disordered nearly all the endoplasmic reticulum (ER) of the notochord cells into irregularly rounded vesicles, but within 3 hours after treatment some parallel arrays of normal endoplasmic reticulum were seen near, and continuous with, the outer nuclear membrane. In addition, a re-ordering of the previously disordered ER took place throughout the cytoplasm, in some cases. A classification was made of the state of the ER as shown in electron micrographs of material fixed immediately, 3, and 24 hours after treatment. This showed that more than half the total endoplasmic reticulum in notochord cells was normal again by 24 hours after treatment.

XII.—Electron Microscopy of the Epithelial Cells of the Mid-gut and Hepatic Cæca of Blatta Orientalis

1961 ◽  
Vol 68 (2) ◽  
pp. 162-170
Author(s):  
L. T. Threadgold ◽  
R. A. R. Gresson
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Epithelial Cells ◽  
Light Microscopy ◽  
Electron Micrographs ◽  
Anterior Part ◽  
Blatta Orientalis ◽  
Lateral Margins

SynopsisElectron micrographs of the anterior part of the mid-gut and hepatic cæca of Blatta orientalis Linn, show epithelial cells in both secretory and absorptive cycles. The epithelial cells possess microvilli and their lateral margins are provided with irregularly shaped projections that interdigitate with neighbouring cells. Vacuole-like structures were observed in association with the mitochondria of absorptive and degenerating cells. These vacuoles, it is suggested, represent degeneration products. Bundles of saccules and associated groups of vacuoles and vesicles represent the Golgi rods and granules of light microscopy. Epithelial cells engaged in absorption differ from those in which the elaboration of secretion is taking place, in the arrangement of the Golgi saccules and vacuoles, in the size of the Golgi vacuoles, in the distribution of the mitochondria, and in the possession of a less extensive endoplasmic reticulum.

Ultrastructure and cytology of pycnia, aecia, and aeciospores of Gymnosporangium clavipes

1975 ◽  
Vol 53 (10) ◽  
pp. 972-977 ◽  
Author(s):  
Frank Kozar ◽  
Hans J. Netolitzky
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Electron Micrographs ◽  
Transmission Electron Micrographs ◽  
Dense Cytoplasm ◽  
Transmission Electron ◽  
Inner Surface ◽  
Scanning Electron

Aeciospores of Gymnosporangium clavipes Cooke & Peck have a surface characterized by a dense covering of baculate projections. Transmission electron micrographs (TEM) reveal a thick non-striated cell wall and a dense cytoplasm. Peridial cells have an inner surface studded with clavate projections. Scanning electron microscope (SEM) microgaphs confirmed earlier light microscopy studies of the existence of fiexious hyphae.

scholarly journals Localization of GTP-stimulated core glycosylation to fused microsomes.

1983 ◽  
Vol 96 (6) ◽  
pp. 1791-1796 ◽  
Author(s):  
J Paiement ◽  
J J Bergeron
Keyword(s):  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Membrane Fusion ◽  
Electron Micrographs ◽  
Microsomal Membrane ◽  
High Concentrations ◽  
Silver Grains ◽  
Acid Labile

Purified rough microsomes from liver maximally incorporated N-acetyl-[3H]glucosamine into endogenous acceptors from UDP-N-acetyl-[3H]glucosamine substrate, providing the associated ribosomes were removed and 0.5 mM GTP was added. These conditions also led to the coalescence of microsomes into large fused membranes. By measurement of membrane profiles on electron micrographs, a correlation was observed between GTP-stimulated glycosylation and microsomal membrane length (r2 = 0.92). Membrane fusion was not observed in the absence of GTP, with sugar transfer inhibited by greater than 90% for acid-resistant acceptors (protein), and approximately 50% for acid-labile acceptors (lipid-linked intermediates). When radiolabeled acceptors were localized by electron microscope radioautography, high concentrations of silver grains (83 grains/100 microns membrane length) were observed over fused membranes with lower grain densities observed over unfused membranes in the same preparation (20 grains/100 microns). These studies directly link microsomal membrane fusion to GTP-stimulated core glycosylation. The observations extend the suggestion of Godelaine et al. (1979, Eur. J. Biochem. 96:17-26) that physiological levels of GTP promote the translocation of substrate across endoplasmic reticulum membranes which, we propose, occurs via a membrane fusion phenomenon.

scholarly journals Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Isei Tanida ◽  
Yoko Furuta ◽  
Junji Yamaguchi ◽  
Soichiro Kakuta ◽  
Juan Alejandro Oliva Trejo ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Electron Micrographs ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Histone H2b ◽  
Thin Sections ◽  
Red Fluorescent Proteins ◽  
Scanning Electron

AbstractIn-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing. Additionally, no study has reported the possibility of two-color in-resin CLEM of Epon embedded cells. In this study, we screened for monomeric green and red fluorescent proteins that resist CLEM processing. We identified mWasabi, CoGFP variant 0, and mCherry2; two green and one red fluorescent proteins as alternatives for in-resin CLEM. We expressed mitochondria-localized mCherry2 and histone H2B tagged with CoGFP variant 0 in cells. Green and red fluorescence was detected in 100 nm-thin sections of the Epon-embedded cells. In the same thin sections, we correlated the fluorescent signals to mitochondria and the nucleus using a scanning electron microscope. Similar results were obtained when endoplasmic reticulum-localized mCherry2 and histone H2B tagged with CoGFP variant 0 were expressed in the cells. Two-color in-resin CLEM of two cytoplasmic organelles, mitochondria and endoplasmic reticulum, was also achieved using mitochondria-localized mCherry2 and endoplasmic reticulum-localized mWasabi. In summary, we report three new fluorescent protein-alternatives suitable for in-resin CLEM of Epon-embedded samples, and achieved Epon-based two-color in-resin CLEM.

Light and electron microscope studies of cytoplasmic aggregates formed in barley cells in response to Erysiphe graminis

1976 ◽  
Vol 54 (14) ◽  
pp. 1647-1655 ◽  
Author(s):  
W. R. Bushnell ◽  
R. J. Zeyen
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Rough Endoplasmic Reticulum ◽  
Rapid Development ◽  
Epidermal Cells ◽  
Erysiphe Graminis ◽  
Synthetic Activity ◽  
Golgi Bodies

Cytoplasmic aggregates that formed in susceptible barley epidermal cells 11-12 h after inoculation with Erysiphe graminis were examined by light microscopy in living specimens and by electron microscopy in fixed specimens. Rapid development of the aggregate (5–10 min) suggested that cytoplasm migrated to the site of each aggregation. The aggregate contained features generally associated with areas of high metabolic and synthetic activity: abundant mitochondria, rough endoplasmic reticulum (associated with smooth cisternae), Golgi bodies, and polyribosomes. Leucoplasts and nuclei were sometimes near aggregates but not consistently. Microbodies and osmiophilic spherosomes were not present.

Utilizing Dark Field Electron Microscopy to Produce Lantern Slides

1974 ◽  
Vol 32 ◽  
pp. 116-117
Author(s):  
J. N. Meador ◽  
C. N. Sun ◽  
H. J. White
Keyword(s):  
Electron Microscope ◽  
Electron Micrographs ◽  
Dark Field ◽  
Limiting Factor ◽  
Clinical Laboratories ◽  
Field Electron ◽  
Time Element ◽  
Field Electron Microscopy ◽  
Dark Field Electron ◽  
Dark Field Micrographs

The electron microscope is being utilized more and more in clinical laboratories for pathologic diagnosis. One of the major problems in the utilization of the electron microscope for diagnostic purposes is the time element involved. Recent experimentation with rapid embedding has shown that this long phase of the process can be greatly shortened. In rush cases the making of projection slides can be eliminated by taking dark field electron micrographs which show up as a positive ready for use. The major limiting factor for use of dark field micrographs is resolution. However, for conference purposes electron micrographs are usually taken at 2.500X to 8.000X. At these low magnifications the resolution obtained is quite acceptable.

The Broad Applications of the Scanning Electron Microscope

1969 ◽  
Vol 27 ◽  
pp. 20-21
Author(s):  
C. T. Nightingale ◽  
S. E. Summers ◽  
T. P. Turnbull
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Surface Topography ◽  
Great Depth ◽  
Resolving Power ◽  
Depth Of Field ◽  
Pictorial Representations ◽  
Scanning Electron

The ease of operation of the scanning electron microscope has insured its wide application in medicine and industry. The micrographs are pictorial representations of surface topography obtained directly from the specimen. The need to replicate is eliminated. The great depth of field and the high resolving power provide far more information than light microscopy.

Copper Localization in Cirrhotic Rat Liver by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 38-39 ◽  
Author(s):  
J. C. Russ ◽  
E. McNatt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Copper Acetate ◽  
Lead Citrate ◽  
Dense Bodies ◽  
Transmission Electron ◽  
Electron Mode ◽  
Scanning Electron

In order to study the retention of copper in cirrhotic liver, rats were made cirrhotic by carbon tetrachloride inhalation twice weekly for three months and fed 0.2% copper acetate ad libidum in drinking water for one month. The liver tissue was fixed in osmium, sectioned approximately 2000 Å thick, and stained with lead citrate. The section was examined in a scanning electron microscope (JEOLCO JSM-2) in the transmission electron mode.Figure 1 shows a typical area that includes a red blood cell in a sinusoid, a disse, and a portion of the cytoplasm of a hepatocyte which contains several mitochondria, peribiliary dense bodies, glycogen granules, and endoplasmic reticulum.

Ultrastructural comparison of prolactin adenomas of pituitary in men and women

1988 ◽  
Vol 46 ◽  
pp. 346-347
Author(s):  
R.C. Caughey ◽  
U.P. Kalyan-Raman
Keyword(s):  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Phosphate Buffer ◽  
Pituitary Adenomas ◽  
Osmium Tetroxide ◽  
Secretory Granules ◽  
Uranyl Acetate ◽  
En Bloc ◽  
Men And Women ◽  
Transmission Electron

Prolactin producing pituitary adenomas are ultrastructurally characterized by secretory granules varying in size (150-300nm), abundance of endoplasmic reticulum, and misplaced exocytosis. They are also subclassified as sparsely or densely granulated according to the amount of granules present. The hormone levels in men and women vary, being higher in men; so also the symptoms vary between both sexes. In order to understand this variation, we studied 21 prolactin producing pituitary adenomas by transmission electron microscope. This was out of a total of 80 pituitary adenomas. There were 6 men and 15 women in this group of 21 prolactinomas.All of the pituitary adenomas were fixed in 2.5% glutaraldehyde, rinsed in Millonig's phosphate buffer, and post fixed with 1% osmium tetroxide. They were then en bloc stained with 0.5% uranyl acetate, rinsed with Walpole's non-phosphate buffer, dehydrated with graded series of ethanols and embedded with Epon 812 epoxy resin.

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