Light and electron microscope studies of cytoplasmic aggregates formed in barley cells in response to Erysiphe graminis

1976 ◽  
Vol 54 (14) ◽  
pp. 1647-1655 ◽  
Author(s):  
W. R. Bushnell ◽  
R. J. Zeyen
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Rough Endoplasmic Reticulum ◽  
Rapid Development ◽  
Epidermal Cells ◽  
Erysiphe Graminis ◽  
Synthetic Activity ◽  
Golgi Bodies

Cytoplasmic aggregates that formed in susceptible barley epidermal cells 11-12 h after inoculation with Erysiphe graminis were examined by light microscopy in living specimens and by electron microscopy in fixed specimens. Rapid development of the aggregate (5–10 min) suggested that cytoplasm migrated to the site of each aggregation. The aggregate contained features generally associated with areas of high metabolic and synthetic activity: abundant mitochondria, rough endoplasmic reticulum (associated with smooth cisternae), Golgi bodies, and polyribosomes. Leucoplasts and nuclei were sometimes near aggregates but not consistently. Microbodies and osmiophilic spherosomes were not present.

scholarly journals AN ELECTRON MICROSCOPE STUDY OF THE ENDOPLASMIC RETICULUM IN NEWT NOTOCHORD CELLS AFTER DISTURBANCE WITH ULTRASONIC TREATMENT AND SUBSEQUENT REGENERATION

1964 ◽  
Vol 20 (1) ◽  
pp. 175-183 ◽  
Author(s):  
G. G. Selman ◽  
A. Jurand
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Ultrasonic Treatment ◽  
Electron Microscope Study ◽  
Triturus Alpestris ◽  
Notochord Cells ◽  
Parallel Arrays ◽  
Subsequent Regeneration

Ultrasonic treatment of the tails of Triturus alpestris tadpoles, at intensities of 8 to 15 watts/cm2, at 1 megacycle/sec., for 5 minutes, disrupted the epidermis and caused pycnosis in individual cells of the muscle and neural tube, but caused no damage to the notochord that could be detected by light microscopy. Electron microscopy showed that this ultrasonic treatment disordered nearly all the endoplasmic reticulum (ER) of the notochord cells into irregularly rounded vesicles, but within 3 hours after treatment some parallel arrays of normal endoplasmic reticulum were seen near, and continuous with, the outer nuclear membrane. In addition, a re-ordering of the previously disordered ER took place throughout the cytoplasm, in some cases. A classification was made of the state of the ER as shown in electron micrographs of material fixed immediately, 3, and 24 hours after treatment. This showed that more than half the total endoplasmic reticulum in notochord cells was normal again by 24 hours after treatment.

scholarly journals Fine structure of the integument of Argas (Persicargas) persicus (Oken) (Ixodoidea: Argasidae)

2005 ◽  
Vol 16 (4) ◽  
Author(s):  
Ashraf Montasser ◽  
Amr Amin
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Fine Structure ◽  
Rough Endoplasmic Reticulum ◽  
Epidermal Cells ◽  
Argas Persicus ◽  
Dermal Glands ◽  
Transmission Electron ◽  
Surface Pores

The integument of Argas persicus was investigated using light, scanning and transmission electron microscopy. The study revealed that two layers, viz. an outer epicuticle and an inner procuticle, form the cuticle. The epicuticle includes wax, cuticulin and protein epicuticular layers. The wax layer carries numerous crater-like deposits, oval or circular discs and numerous infoldings. The procuticle contains an exo-, endo- and a subcuticle.Underlining the cuticle, flattened epidermal cells are connected via desmosomes and contain rough endoplasmic reticulum, free ribosomes and mitochondria. Scattered dermal glands are located beneath the cuticle and are continuous with the outside through dermal ducts and surface pores.

Modification of Pineal Ultrastructure by Exogenous Hormones

1967 ◽  
Vol 25 ◽  
pp. 170-171
Author(s):  
R. A. Turner ◽  
A. E. Rodin ◽  
D. K. Roberts
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Estradiol Benzoate ◽  
Female Rats ◽  
List Type ◽  
Type Number ◽  
Pregnant Rats ◽  
Gonadal Atrophy ◽  
Pineal Ultrastructure

There have been many reports which establish a relationship between the pineal and sexual structures, including gonadal hypertrophy after pinealectomy, and gonadal atrophy after injection of pineal homogenates or of melatonin. In order to further delineate this relationship the pineals from 5 groups of female rats were studied by electron microscopy:ControlsPregnant ratsAfter 4 weekly injections of 0.1 mg. estradiol benzoate.After 8 daily injections of 150 mcgm. melatonin (pineal hormone).After 8 daily injections of 3 mg. serotonin (melatonin precursor).No ultrastructural differences were evident between the control, and the pregnancy and melatonin groups. However, the estradiol injected animals exhibited a marked increase in the amount and size of rough endoplasmic reticulum within the pineal cells.

Copper Localization in Cirrhotic Rat Liver by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 38-39 ◽  
Author(s):  
J. C. Russ ◽  
E. McNatt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Copper Acetate ◽  
Lead Citrate ◽  
Dense Bodies ◽  
Transmission Electron ◽  
Electron Mode ◽  
Scanning Electron

In order to study the retention of copper in cirrhotic liver, rats were made cirrhotic by carbon tetrachloride inhalation twice weekly for three months and fed 0.2% copper acetate ad libidum in drinking water for one month. The liver tissue was fixed in osmium, sectioned approximately 2000 Å thick, and stained with lead citrate. The section was examined in a scanning electron microscope (JEOLCO JSM-2) in the transmission electron mode.Figure 1 shows a typical area that includes a red blood cell in a sinusoid, a disse, and a portion of the cytoplasm of a hepatocyte which contains several mitochondria, peribiliary dense bodies, glycogen granules, and endoplasmic reticulum.

Selective Sampling and Orientation of Non-Homogeneous Tissues

1968 ◽  
Vol 26 ◽  
pp. 98-99
Author(s):  
C. C. Clawson ◽  
L. W. Anderson ◽  
R. A. Good
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Random Sampling ◽  
New Method ◽  
Microscope Examination ◽  
Small Areas ◽  
Selective Sampling ◽  
Large Numbers ◽  
Specific Orientation

Investigations which require electron microscope examination of a few specific areas of non-homogeneous tissues make random sampling of small blocks an inefficient and unrewarding procedure. Therefore, several investigators have devised methods which allow obtaining sample blocks for electron microscopy from region of tissue previously identified by light microscopy of present here techniques which make possible: 1) sampling tissue for electron microscopy from selected areas previously identified by light microscopy of relatively large pieces of tissue; 2) dehydration and embedding large numbers of individually identified blocks while keeping each one separate; 3) a new method of maintaining specific orientation of blocks during embedding; 4) special light microscopic staining or fluorescent procedures and electron microscopy on immediately adjacent small areas of tissue.

Characterization of submicrometer fluid Inclusions in minerals by Analytical/Transmission Electron Microscopy and electron diffraction

1990 ◽  
Vol 48 (4) ◽  
pp. 424-424
Author(s):  
George Guthrie ◽  
David Veblen
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Microscope ◽  
Electron Diffraction ◽  
Light Microscopy ◽  
Fluid Inclusions ◽  
Energy Dispersive Spectrometer ◽  
Analytical Transmission Electron Microscopy ◽  
Transmission Electron

The nature of a geologic fluid can often be inferred from fluid-filled cavities (generally <100 μm in size) that are trapped during the growth of a mineral. A variety of techniques enables the fluids and daughter crystals (any solid precipitated from the trapped fluid) to be identified from cavities greater than a few micrometers. Many minerals, however, contain fluid inclusions smaller than a micrometer. Though inclusions this small are difficult or impossible to study by conventional techniques, they are ideally suited for study by analytical/ transmission electron microscopy (A/TEM) and electron diffraction. We have used this technique to study fluid inclusions and daughter crystals in diamond and feldspar.Inclusion-rich samples of diamond and feldspar were ion-thinned to electron transparency and examined with a Philips 420T electron microscope (120 keV) equipped with an EDAX beryllium-windowed energy dispersive spectrometer. Thin edges of the sample were perforated in areas that appeared in light microscopy to be populated densely with inclusions. In a few cases, the perforations were bound polygonal sides to which crystals (structurally and compositionally different from the host mineral) were attached (Figure 1).

scholarly journals ELECTRON MICROSCOPY OF CENTRIFUGED HYPHAE OF NEUROSPORA

1961 ◽  
Vol 9 (3) ◽  
pp. 609-617 ◽  
Author(s):  
M. Zalokar
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Optical Microscope ◽  
Granular Structure ◽  
Cell Physiology ◽  
Cell Structures ◽  
Light Area ◽  
Fat Bodies

Normal and centrifuged hyphae of Neurospora were studied with the electron microscope. The following cell structures could be identified: nuclei with nucleoli, mitochondria, endoplasmic reticulum, ribosomes, glycogen, fat bodies, vacuoles, and vesicles with an inner canalicular system, of unknown nature. In centrifuged hyphae, the glycogen layer appeared as a light area, with a slight indication of granular structure. The ribosome layer consisted of densely packed ribosomes without any membranes. The mitochondrial layer contained spaces filled with ribosomes. The nuclei were loosely packed, with endoplasmic reticulum between them. The "enchylema" layer was composed of vesicles belonging to the endoplasmic reticulum. The vacuolar layer was poorly preserved and consisted of double-walled vesicles. Fat appeared as stellate osmiophilic droplets. These observations were compared with previous observations under the optical microscope and their meaning for cell physiology was discussed.

The Tegument and Associated Structures of Fasciola Hepatica

1963 ◽  
Vol s3-104 (68) ◽  
pp. 505-512
Author(s):  
L. T. THREADGOLD
Keyword(s):  
Electron Microscopy ◽  
Surface Layer ◽  
Light Microscopy ◽  
Fasciola Hepatica ◽  
Golgi Bodies ◽  
New Knowledge ◽  
Pinocytotic Vesicles ◽  
The Individual ◽  
And Function ◽  
Internal Level

The cuticle of light microscopy is shown by electron microscopy to be a surface layer of protoplasm which is an extension of areas of nucleated protoplasm lying deep in the parenchyma. The cuticle therefore exists at two levels. The external level is syncytial, consisting of plateaux separated by branching valleys. This level contains apical pinocytotic vesicles, numerous mitochondria, endoplasmic membranes, large basal and other vacuoles, and dense spines. Tube-like evaginations from the base of the external level connect it to the individual areas of flask-shaped protoplasm which compose the internal level. Each of these areas of protoplasm contains a nucleus, great numbers of mitochondria, some vacuoles and diffuse inclusions, and the Golgi bodies. The histochemistry and function of the cuticle is discussed in the light of this new knowledge of cuticular ultrastructure, and a comparison is made between the cuticle of Cestoda and Trematoda.

In vitro development of isolated ectoderm from axolotl gastrulae

Development ◽  
1984 ◽  
Vol 80 (1) ◽  
pp. 321-330
Author(s):  
Jonathan M. W. Slack
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Outer Layer ◽  
Microscope Examination ◽  
Animal Pole ◽  
In Vitro Development ◽  
Electron Microscope Examination ◽  
Vitro Development

The development of ectoderm isolated from the animal pole of axolotl gastrulae is monitored by light microscopy, electron microscopy and analysis of newly synthesized proteins, glycoproteins and glycolipids. When control embryos are undergoing neurulation it is shown that the explants autonomously begin to express epidermal markers and do not express mesodermal markers. However the results suggest that not all the cells become epidermal and electron microscope examination shows that only the outer layer does so, the inner cells remaining undifferentiated.

scholarly journals Ultrastructural studies of the hemocytes of Panstrongylus megistus (Hemiptera: Reduvidae)

1989 ◽  
Vol 84 (2) ◽  
pp. 171-188 ◽  
Author(s):  
Margherita A. Barracco ◽  
Clarice T. Loch
Keyword(s):  
Endoplasmic Reticulum ◽  
Light Microscopy ◽  
Rough Endoplasmic Reticulum ◽  
Lipid Droplets ◽  
Panstrongylus Megistus ◽  
Ultrastructural Studies ◽  
Hemocyte Count

Ultrastructural analyses revealed the presence of six hemocyte types in the hemolymph of Panstrogylus megistus, partially confirming our previous results obtained through light microscopy. Prohemocytes: small, round hemocytes with a thin cytoplasm layer, espcieally rich in free ribosomes and poor in membranous systems. Plasmatocytes: polymorphic cells, whose cytoplasm contains many lysosomes and a well developed rough endoplasmic reticulum (RER).They are extremely phagocytic. Sometimes, they show a large vacuolation. Granulocytes: granular hemocytes whose granules show different degrees of electrondensity. Most of them, have an internal structuration. Coagulocytes: oval or elongated hemocytes, which show pronounced perinuclear cisternae as normally observed in coagulocytes. The cytoplasm is usually electrondense, poor in membranous systems and contains many labile granules. Oenocytoids: large and very stable hemocytes, whose homogeneous cytoplasme is rich in loose ribosomes and poor in membranous systems. Adipohemocytes: large cells, containing several characteristic lipid droplets. The cytoplasm is also rich in glycogen, RER and large mitochondria. The total and differential hemocyte count (THC and DHC) were also calculated for this reduviid. THC increases from 2,900 hemocytes/cubic millimeter of hemolymph in the 4th intar to 4,350 in the 5th and then, decreases to 1,950 in the adults. Plasmatocytes and coagulocytes are the predominant hemocyte types.

Sign in / Sign up

Export Citation Format

Share Document