Anther and pollen infection in relation to the pollen and seed transmissibility of two strains of barley stripe mosaic virus in barley

1976 ◽  
Vol 54 (14) ◽  
pp. 1604-1621 ◽  
Author(s):  
Thomas W. Carroll ◽  
Dennis E. Mayhew
Keyword(s):  
Mosaic Virus ◽  
Pollen Development ◽  
Floral Meristem ◽  
Barley Stripe Mosaic Virus ◽  
Seed Transmissibility ◽  
Different Strains ◽  
Mother Cells ◽  
Mitosis And Meiosis ◽  
Normal Mitosis ◽  
Mature Anther

Anther and pollen infection in relation to seed and pollen transmission were studied for two different strains of barley stripe mosaic virus in 'Atlas' barley. Examination of sectioned anthers and pollen revealed that the seed- and pollen-transmitted isolate of the virus (MI-1) invaded the floral meristem of the host early and subsequently infected the pollen mother cells and sperms. During the premeiotic and meiotic stages of anther and pollen development, most virions of MI-1 were seen attached to microtubules, including those of the spindle. In later stages, the association of virions with microtubules diminished. Usually, MI-1 caused no apparent interference with normal mitosis and meiosis during development of anthers and pollen in central florets. Occasionally, however, the virus induced degeneration in anther and pollen-precursor cells before and during meiosis. By comparison, virions of the NSP strain, a strain which is not seed or pollen transmitted, could only be detected in wall cells of a single mature anther. None were discovered in the floral meristem or in pollen. It was also shown with unsectioned material that infection with either virus adversely affected anther and pollen development and that infection by MI-1 increased seed sterility.

Occurrence of virions in developing ovules and embryo sacs of barley in relation to the seed transmissibility of barley stripe mosaic virus

1976 ◽  
Vol 54 (21) ◽  
pp. 2497-2512 ◽  
Author(s):  
Thomas W. Carroll ◽  
Dennis E. Mayhew
Keyword(s):  
Mosaic Virus ◽  
Microscopic Examination ◽  
Embryo Sac ◽  
Electron Microscopic Examination ◽  
Barley Stripe Mosaic Virus ◽  
Ovule Development ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Seed Transmissibility ◽  
Spindle Microtubules

Electron microscopic examination revealed the occurrence of virions in thin sections of developing ovules and embryo sacs of Atlas barley infected with a seed-transmitted strain of barley stripe mosaic virus, MI-1. It appeared that the virus invaded the primaiy meristem early, then infected the megaspore mother cell. In later stages of ovule development, the virus was seen in megaspores and in the cells of the embryo sac, including the egg. Virions were commonly associated with wall, cytoplasmic, or spindle microtubules. By contrast, virions of the non-seed-transmitted strain of the virus (NSP) did not occur in developing ovules or embryo sacs. Ovule transmission was only demonstrated for MI-1.

scholarly journals Role of Soybean mosaic virus–Encoded Proteins in Seed and Aphid Transmission in Soybean

2013 ◽  
Vol 103 (9) ◽  
pp. 941-948 ◽  
Author(s):  
Sushma Jossey ◽  
Houston A. Hobbs ◽  
Leslie L. Domier
Keyword(s):  
Mosaic Virus ◽  
Seed Quality ◽  
Soybean Mosaic Virus ◽  
Seed Transmission ◽  
Aphid Transmission ◽  
Plant Introduction ◽  
Sequence Motif ◽  
Coding Region ◽  
Encoded Proteins ◽  
Seed Transmissibility

Soybean mosaic virus (SMV) is seed and aphid transmitted and can cause significant reductions in yield and seed quality in soybean (Glycine max). The roles in seed and aphid transmission of selected SMV-encoded proteins were investigated by constructing mutants in and chimeric recombinants between SMV 413 (efficiently aphid and seed transmitted) and an isolate of SMV G2 (not aphid or seed transmitted). As previously reported, the DAG amino acid sequence motif near the amino terminus of the coat protein (CP) was the major determinant in differences in aphid transmissibility of the two SMV isolates, and helper component proteinase (HC-Pro) played a secondary role. Seed transmission of SMV was influenced by P1, HC-Pro, and CP. Replacement of the P1 coding region of SMV 413 with that of SMV G2 significantly enhanced seed transmissibility of SMV 413. Substitution in SMV 413 of the two amino acids that varied in the CPs of the two isolates with those from SMV G2, G to D in the DAG motif and Q to P near the carboxyl terminus, significantly reduced seed transmission. The Q-to-P substitution in SMV 413 also abolished virus-induced seed-coat mottling in plant introduction 68671. This is the first report associating P1, CP, and the DAG motif with seed transmission of a potyvirus and suggests that HC-Pro interactions with CP are important for multiple functions in the virus infection cycle.

Comparisons of poa semilatent and barley stripe mosaic viruses

1972 ◽  
Vol 50 (2) ◽  
pp. 263-267 ◽  
Author(s):  
Z. Polák ◽  
J. T. Slykhuis
Keyword(s):  
Mosaic Virus ◽  
Double Diffusion ◽  
Barley Stripe Mosaic Virus ◽  
Diffusion Test ◽  
Partial Protection ◽  
Normal Length ◽  
Local Lesions ◽  
Cross Absorption ◽  
Precipitin Test ◽  
Agropyron Trachycaulum

Poa semilatent virus (PSLV), which caused chlorosis and rapid death of inoculated wheat, infected a number of grasses susceptible to barley stripe mosaic virus (BSMV); but it also infected Agropyron trachycaulum and Poa palustris which were not susceptible to a barley strain or two oat-infecting strains of BSMV. Conversely BSMV caused local lesions on several species of Chenopodium that did not become infected with PSLV. BSMV protected wheat from infection by PSLV, but PSLV caused only partial protection from BSMV.The normal length and thickness of particles in leaf-dip preparations was 161 × 26 mμ for PSLV and 133 × 25 mμ for BSMV.Serological relationship was not indicated by the Ouchterlony agar double-diffusion test or leaf-dip serology. A distant relationship was shown with the microprecipitin test and the ring interface precipitin test. In cross absorption tests the titers of the antisera to the homologous viruses were not reduced by absorption with the heterologous viruses.PSLV and BSMV appear to be distantly related serotypes.

scholarly journals Barley Stripe Mosaic Virus (BSMV) Induced MicroRNA Silencing in Common Wheat (Triticum aestivum L.)

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0126621 ◽  
Author(s):  
Jian Jiao ◽  
Yichun Wang ◽  
Jonathan Nimal Selvaraj ◽  
Fuguo Xing ◽  
Yang Liu
Keyword(s):  
Triticum Aestivum ◽  
Mosaic Virus ◽  
Common Wheat ◽  
Triticum Aestivum L ◽  
Barley Stripe Mosaic Virus

scholarly journals Barley Stripe Mosaic Virus γb Interacts with Glycolate Oxidase and Inhibits Peroxisomal ROS Production to Facilitate Virus Infection

2018 ◽  
Vol 11 (2) ◽  
pp. 338-341 ◽  
Author(s):  
Meng Yang ◽  
Zhenggang Li ◽  
Kun Zhang ◽  
Xuan Zhang ◽  
Yongliang Zhang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Glycolate Oxidase ◽  
Barley Stripe Mosaic Virus ◽  
Ros Production

scholarly journals Subcellular Localization of the Barley Stripe Mosaic Virus Triple Gene Block Proteins

2009 ◽  
Vol 83 (21) ◽  
pp. 11413-11413 ◽  
Author(s):  
Hyoun-Sub Lim ◽  
Jennifer N. Bragg ◽  
Uma Ganesan ◽  
Steven Ruzin ◽  
Denise Schichnes ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Mosaic Virus ◽  
Triple Gene Block ◽  
Barley Stripe Mosaic Virus ◽  
Gene Block
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