Growth and metabolism of cells and tissue of jack pine (Pinus banksiana). 7. Observations on cytoplasmic streaming and effects of L-glutamine and its analogues on subcellular activities

1976 ◽  
Vol 54 (5-6) ◽  
pp. 507-517 ◽  
Author(s):  
D. J. Durzan ◽  
G. Bourgon
Keyword(s):  
Pinus Banksiana ◽  
Cytoplasmic Streaming ◽  
Basal Medium ◽  
Jack Pine ◽  
Concomitant Increase ◽  
Low Levels ◽  
Nuclear Behaviour ◽  
Transvacuolar Strands ◽  
Nucleolar Size ◽  
Streaming Motion

Cells from jack pine (Pinus banksiana Lamb.) callus and suspension cultures were observed by cinemicrography to evaluate cytoplasmic streaming, motion of organelles, and nuclear behaviour under glutamine stress. Streaming rates were greatest in the largest cells. Rates were reduced in smaller cells, where transvacuolar strands were not as prominent and displacements of organelles were not strongly affected by cyclosis. During increased rates of cytoplasmic streaming, chloroplasts tended to associate with each other and mitochondria often changed direction and shape.The addition of 400 ppm L-glutamine to a defined basal medium increased the linear velocity of organelles from 2-7 microns (μ)/s to 7–12 μ/s, whereas D-glutamine eventually stopped cytoplasmic streaming, with a concomitant increase in nucleolar size. While additions of low levels of α-N-acetyl-L-glutamine and isoglutamine stimulated streaming, this occurred usually without other significant morphological effects. Cytoplasmic movements that were induced by exposure of cells to L-glutamine were intimately involved with the fine strands and with nuclear and nucleolar behavior. Movements ceased when strands were disrupted under nutritional stress and by inhibitory responses to D-glutamine.

Growth and metabolism of cells and tissue of jack pine (Pinus banksiana). 3. Growth of cells in liquid suspension cultures in light and darkness

1976 ◽  
Vol 54 (5-6) ◽  
pp. 456-467 ◽  
Author(s):  
D. J. Durzan ◽  
V. Chalupa
Keyword(s):  
Pinus Banksiana ◽  
Basal Medium ◽  
Continuous Light ◽  
Cell Suspension Cultures ◽  
Jack Pine ◽  
Suspension Cultures ◽  
Growth Patterns ◽  
Final Size ◽  
Daughter Cells ◽  
Liquid Suspension

Friable, jack pine (Pinus banksiana Lamb.) callus on agar plates with a defined nutrient medium was transferred to a liquid medium of the same composition to establish cell suspension cultures. Dry weights and patterns of growth for daughter cells were estimated in continuous light or darkness, with or without conditioned media and arginine supplement. In all treatments, growth was near-exponential. Greatest final size was obtained with basal medium under continuous light.The distribution of clump sizes at the later stages of growth fits a stochastic model reflecting two types of daughter cell behavior described by the probability of one type remaining with the clump as opposed to sloughing off into the medium. In light, cells contained many more chloroplasts, thicker cell walls, and more compacted clumps than in darkness: yet cellular clumping patterns both in light and darkness were largely similar. Nearly 20% of the clumps showed polarity and symmetry and contained actively streaming suspensor-like cells. The remaining clumps were spherical and produced unorganized growth patterns. The progress of growth was eventually dominated by the tendency of daughter cells to remain attached to clumps compared with the initial tendency for clumps to fragment and cells to be released into the medium.

Pyrimidine metabolism in seeds and seedlings of jack pine (Pinus banksiana)

1975 ◽  
Vol 53 (7) ◽  
pp. 673-686 ◽  
Author(s):  
J. Pitel ◽  
D. J. Durzan
Keyword(s):  
Nucleic Acids ◽  
Dna Synthesis ◽  
Pinus Banksiana ◽  
Orotic Acid ◽  
De Novo ◽  
Jack Pine ◽  
Pyrimidine Metabolism ◽  
De Novo Synthesis ◽  
Pine Seedlings ◽  
Low Levels

Jack pine seedlings rapidly converted radioactive thymine-6-3H to dihydrothymine, β-ureidoisobutyric acid, and β-aminoisobutyric acid. Autoradiographs of hypocotyl cells showed radioactivity largely in the cytoplasm. A small percentage of radioactivity from thymine-2-14C was salvaged via uracil for the synthesis of RNA. DNA was not labelled significantly. The occurrence of a mechanism to utilize thymidine-methyl-3H for DNA synthesis was detected at low levels in germinating seedlings but was absent in seeds up to 16 h imbibition. Among pyrimidines tested thymidine was the most specific for the labelling of DNA and radioactivity was detected almost entirely over nuclei.The occurrence of an active pathway for the de novo synthesis of the pyrimidine portions of nucleic acids was demonstrated by the use of orotic acid-4-14C. Native DNA and all major types of RNA were labelled and radioactivity resided in pyrimidines. Synthesis of DNA involved the conversion of pyrimidine ribonucleotides to deoxyribonucleotides.

scholarly journals Trade-Offs among Release Treatments in Jack Pine Plantations: Twenty-Five Year Responses

Forests ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 370
Author(s):  
Holly D. Deighton ◽  
Frederick Wayne Bell ◽  
Nelson Thiffault ◽  
Eric B. Searle ◽  
Mathew Leitch ◽  
...  
Keyword(s):  
Plant Diversity ◽  
Boreal Forests ◽  
Pinus Banksiana ◽  
Vegetation Management ◽  
Jack Pine ◽  
Forest Diversity ◽  
Trade Offs ◽  
Jack Pine Forests ◽  
Stand Yield ◽  
Forest Managers

We assessed 27 indicators of plant diversity, stand yield and individual crop tree responses 25 years post-treatment to determine long-term trade-offs among conifer release treatments in boreal and sub-boreal forests. This research addresses the lack of longer-term data needed by forest managers to implement more integrated vegetation management programs, supporting more informed decisions about release treatment choice. Four treatments (untreated control, motor-manual brushsaw, single aerial spray, and complete competition removal) were established at two jack pine (Pinus banksiana Lamb.) sites in Ontario, Canada. Our results suggest that plant diversity and productivity in boreal jack pine forests are significantly influenced by vegetation management treatments. Overall, release treatments did not cause a loss of diversity but benefitted stand-scale yield and individual crop tree growth, with maximum benefits occurring in more intensive release treatments. However, none of the treatments maximized all 27 indicators studied; thus, forest managers are faced with trade-offs when choosing treatments. Research on longer term effects, ideally through at least one rotation, is essential to fully understand outcomes of different vegetation management on forest diversity, stand yield, and individual crop tree responses.

Jack pine cone quality and cache sizes of red squirrels in southern New Brunswick, Canada

1997 ◽  
Vol 75 (2) ◽  
pp. 332-335 ◽  
Author(s):  
Michael A. Setterington ◽  
Daniel M. Keppie
Keyword(s):  
New Brunswick ◽  
Pinus Banksiana ◽  
Seed Mass ◽  
Dry Mass ◽  
Jack Pine ◽  
Tamiasciurus Hudsonicus ◽  
Red Squirrels ◽  
Length Width ◽  
Total Seed ◽  
Number Of Seeds

Relationships between external cone characteristics (length, width, wet and dry mass), cone quality (total seed mass as a proportion of cone mass, total number of seeds per cone, total seed mass per cone), and number of cones in caches were evaluated for caches of jack pine (Pinus banksiana) cones belonging to red squirrels (Tamiasciurus hudsonicus) in two plantations in southern New Brunswick. Cone length and mass were good predictors of the total number of seeds per cone and total seed mass per cone. Length accounted for a small proportion of the variance of total seed mass as a proportion of cone mass. There was no relationship between the number of seeds or total seed mass per cone and the number of cones per cache.

scholarly journals Genetically based resistance to the white pine weevil in jack pine and eastern white pine

2010 ◽  
Vol 86 (6) ◽  
pp. 775-779 ◽  
Author(s):  
Alice Verrez ◽  
Dan Quiring ◽  
Thibaut Leinekugel Le Cocq ◽  
Greg Adams ◽  
Yill Sung Park
Keyword(s):  
Pinus Banksiana ◽  
Significant Proportion ◽  
Tree Height ◽  
Jack Pine ◽  
White Pine ◽  
Pissodes Strobi ◽  
Pine Weevil ◽  
Tree Resistance ◽  
Trade Offs ◽  
White Pine Weevil

White pine weevil (Pissodes strobi Peck) damage was evaluated in one white pine (Pinus strobus L.) and four jack pine(Pinus banksiana Lamb) half-sib family test sites to determine the role of tree genotype in resistance to the weevil. Halfsibfamily explained a significant proportion of the variation in weevil attack at all sites. Estimates of family (0.16-0.54)and individual (0.09-0.24) heritabilities of jack pine resistance to white pine weevil were moderate. Estimates of family(0.37) and individual (0.22) heritability of resistance of white pine to the weevil were also moderate when the percentageof test trees damaged by the weevil was relatively low, but were insignificant four years later when more than three-quartersof trees were damaged. Significant positive correlations between mean tree height and mean incidence of trees damagedby the weevil were observed for four of seven site-years but relationships were weak, suggesting that any cost, withrespect to height growth, to breeding weevil resistant trees may be small.Key words: Pinus, Pissodes strobi, trade-offs, tree improvement, tree resistance, white pine weevil.

Spatial genetic substructure within natural populations of jack pine (Pinus banksiana)

1991 ◽  
Vol 69 (3) ◽  
pp. 547-551 ◽  
Author(s):  
Chang Yi Xie ◽  
Peggy Knowles
Keyword(s):  
Spatial Autocorrelation ◽  
Pinus Banksiana ◽  
Pollen Dispersal ◽  
Natural Populations ◽  
Jack Pine ◽  
Spatial Autocorrelation Analysis ◽  
Autocorrelation Analysis ◽  
Long Distance ◽  
Genetic Substructure ◽  
Good Agreement

Spatial autocorrelation analysis was used to investigate the geographic distribution of allozyme genotypes within three natural populations of jack pine (Pinus banksiana Lamb.). Results indicate that genetic substructuring within these populations is very weak and the extent differs among populations. These results are in good agreement with those inferred from mating-system studies. Factors such as the species' predominantly outbreeding system, high mortality of selfs and inbreds prior to reproduction, long-distance pollen dispersal, and the absence of strong microhabitat selection may be responsible for the observed weak genetic substructuring. Key words: jack pine, Pinus banksiana, genetic substructure, allozyme, spatial autocorrelation analysis.

Alternate Strip Clearcutting in Upland Black Spruce: III. Regeneration Options for Leave Strips

1987 ◽  
Vol 63 (6) ◽  
pp. 446-450 ◽  
Author(s):  
James E. Wood ◽  
Richard Raper
Keyword(s):  
Pinus Banksiana ◽  
Black Spruce ◽  
Cost Savings ◽  
Jack Pine ◽  
Direct Seeding ◽  
Transparent Plastic ◽  
Management Objectives ◽  
Biological Criteria ◽  
Alternative Sources ◽  
Topographic Positions

In the alternate strip clearcutting system, first-cut strips are regenerated by seed produced by black spruce (Picea mariana [Mill.] B.S.P.) in the forested leave strips. However, after the second cut, such a seed source is not available for regenerating the leave strips. Therefore, the forest manager must consider a number of alternative regeneration options. The selection of the most appropriate regeneration option is dependent upon several economic and biological criteria. These include future costs of delivered wood, site productivity, post-harvest site condition, future alternative sources of supply, and future demand for industrial wood. Regeneration options such as preservation of advance growth and direct seeding are recommended for sites on which the manager is concerned primarily with regenerating first cut strips and is willing to accept a lower level of stocking in leave strips. Planting, the most intensive option discussed, should be reserved for sites offering the highest potential return or greatest future cost savings. Direct seeding of jack pine (Pinus banksiana Lamb.) should be considered on the upland portions of this patterned site type. Mixing jack pine and black spruce is a suggested regeneration option if the site contains both upland and lowland topographic positions. Other seeding options include the use of semi-transparent plastic seed shelters. The manager might consider combining two or more of these options to meet management objectives.

THE SUPPRESSION OF GROWTH RINGS IN JACK PINE IN RELATION TO DEFOLIATION BY THE SWAINE JACK-PINE SAWFLY

1963 ◽  
Vol 41 (2) ◽  
pp. 227-235 ◽  
Author(s):  
L. C. O'Neil
Keyword(s):  
Pinus Banksiana ◽  
Radial Growth ◽  
Jack Pine ◽  
Growth Rings ◽  
Cross Sectional ◽  
Pine Sawfly ◽  
Young Stand ◽  
The Dead ◽  
Dense Stand

An investigation of the radial growth of jack pine (Pinus banksiana Lamb.) defoliated by the Swaine jack-pine sawfly (Neodiprion swainei Midd.) disclosed that growth rings were discontinuous and missing in cross-sectional disks from severely damaged trees. In young and open-grown trees with dead tops, the incidence of such deficiencies in radial growth was especially high in disks from upper regions of the stems, in the vicinity of the dead tops; radial growth was suspended for 1 year and subsequently resumed in disks from the lower regions of some stems. Cambial inactivity was more generalized in trees from an old and dense stand and it was detected in disks representing major portions of some of the stems sampled; the death of some trees followed 2 to 6 years of cambial inactivity in disks cut at various heights along their entire stems. Growth deficiencies in the young stand were clearly effects of severe sawfly defoliation. Data from the old, dense stand indicated that sawfly defoliation had perhaps merely hastened the gradual deterioration of the stand in which intertree competition was intense.

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