Occurrence and levels of the phytoalexin phaseollin in relation to delimitation at sites of infection of Phaseolus vulgaris by Colletotrichum lindemuthianum

1973 ◽  
Vol 51 (12) ◽  
pp. 2423-2430 ◽  
Author(s):  
J. E. Rahe
Keyword(s):  
Phaseolus Vulgaris ◽  
Host Response ◽  
Healthy Tissue ◽  
Colletotrichum Lindemuthianum ◽  
Incompatible Interaction ◽  
Interaction Type ◽  
Incompatible Response ◽  
Primary Determinant ◽  
Quantitative Analyses ◽  
The Difference

Sequential quantitative analyses of phaseollin associated with individual sites of infection by Colletotrichum lindemuthianum on etiolated hypocotyls of Phaseolus vulgaris provide further insight concerning the possible function of phaseollin in compatible and incompatible host response. When detected, phaseollin was localized in and (or) around individual infection sites; it was not detected in adjacent healthy tissue. Its occurrence was correlated with the time of appearance of visible, necrotic flecks at sites of incompatible response. It was not detected at visibly necrotic sites of compatible response until these became delimited in appearance, after which it occurred in large amounts. Delimitation at these sites occurred well after visible necrosis was first apparent. At high levels of infection, individual sites of compatible infection coalesced before delimitation, and in such instances phaseollin was virtually absent for the duration of the experiments. The levels of phaseollin occurring at individual infection sites indicate that it does not function as the primary determinant of interaction type, but is associated with delimitation at sites of both compatible and incompatible interaction. With respect to phaseollin production, the difference between these interactions was clearly one of time of occurrence, rather than amount produced.

Effects of 15% CO2 on the susceptibility of Phaseolus vulgaris to Colletotrichum lindemuthianum

1976 ◽  
Vol 54 (10) ◽  
pp. 1035-1043 ◽  
Author(s):  
Robert M. Arnold ◽  
James E. Rahe
Keyword(s):  
Phaseolus Vulgaris ◽  
Defense Response ◽  
Colletotrichum Lindemuthianum ◽  
Host Cells ◽  
Incompatible Interaction ◽  
Stages Of Development ◽  
Histological Studies ◽  
Slowing Down ◽  
Fungal Hyphae ◽  
Complete Reversal

Changes in the nature of the interaction between Phaseolus vulgaris and a compatible race of Colletotrichum lindemuthianum were obtained by treatment of inoculated plants with 15% CO2 in air. A shift towards resistance, manifested by the appearance of flecks similar to those which develop in a natural incompatible interaction, occurred on plants placed in CO2 during the early stages of fungal penetration and maintained there until symptoms appeared. With such treatment, almost complete reversal of the interaction was obtained when the density of infection sites was sufficiently great to give coalescent necrosis in controls that were not CO2-treated. A lesser effect occurred when CO2 treatment was begun at later stages of development of the interaction. Histological studies showed that the rate of colonization of host cells by hyphae of the fungus was reduced in the presence of 15% CO2. These results indicate that CO2 may change the nature of the interaction to one of resistance by selectively slowing down the growth of the fungus while allowing a defense response of the host to develop to a degree where it can prevent further spread of fungal hyphae.

scholarly journals The histidine kinase slnCl1 of Colletotrichum lindemuthianum as a pathogenicity factor against Phaseolus vulgaris L

2019 ◽  
Vol 219 ◽  
pp. 110-122 ◽  
Author(s):  
Guilherme Bicalho Nogueira ◽  
Leandro Vieira dos Santos ◽  
Casley Borges de Queiroz ◽  
Thamy Lívia Ribeiro Corrêa ◽  
Renato Pedrozo Menicucci ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Histidine Kinase ◽  
Colletotrichum Lindemuthianum ◽  
Phaseolus Vulgaris L ◽  
Pathogenicity Factor

scholarly journals Avaliação da produtividade de linhagens de feijoeiro resistentes ao fungo da antracnose

Bragantia ◽  
1982 ◽  
Vol 41 (1) ◽  
pp. 67-79 ◽  
Author(s):  
Antonio Sidney Pompeu
Keyword(s):  
Phaseolus Vulgaris ◽  
Colletotrichum Lindemuthianum

A capacidade produtiva de linhagens de feijoeiro (Phaseolus vulgaris) resistentes ao fungo da antracnose (Colletotrichum lindemuthianum) pertencentes aos grupos Preto, Chumbinho e Diversas, foi estimada em experimentos em blocos ao acaso, instalados em Campinas, no cultivo das águas de 1977, 1978 e 1979. Utilizaram-se os cultivares Moruna, Aroana e Carioca como controles para as linhagens dos grupos Preto, Chumbinho e Diversos respectivamente. Pela análise estatística dos dados obtidos pelas linhagens do grupo Preto, comuns aos ensaios de 1977 e 1978, 1978 e 1979, observou-se que as de prefixos 5-9-4-3-1-4, 5-9-4-3-1-5, 18-1-6 e 5-9-4-3-1-6, com médias de 2.100, 2.079, 2.055 e 2.028kg/ha, foram superiores ao 'Moruna', que produziu 1.580kg/ha. No grupo Chumbinho, cujas linhagens foram avaliadas em 1978 e 1979, a de prefixo 5-1-1-5-1-9, com 2.336kg/ha, destacou-se das demais, embora esta produtividade média estivesse ao mesmo nível, estatisticamente, da verificada para o 'Aroana', 2.136kg/ha. Para as linhagens do grupo Diversos, estudadas em 1978 e 1979, apenas 10-3-1 e 10-6-2, com produções médias de 3.133 e 2.823kg/ha, foram superiores, respectivamente, a 2.375 e 2.355kg/ha, observadas para o 'Carioca'. Outras linhagens deste grupo tiveram médias acima do controle, como 10-9-2, 10-9-1 e 10-5-1, com 2.698, 2.686 e 2.649kg/ha (Carioca - 2.355kg/ha) na ausência do patógeno. Nas análises conjuntas foram notados efeitos significativos para ano em onze dos doze grupos de experimentos e em apenas dois para a interação tratamento x ano. As melhores linhagens serão colocadas nos ensaios regionais e algumas terão suas sementes aumentadas e colocadas à disposição dos agricultores da região de Campinas.

scholarly journals Utilização da produtividade de grãos na seleção para resistência ao Colletotrichum lindemuthianum no feijoeiro

2003 ◽  
Vol 27 (2) ◽  
pp. 363-369 ◽  
Author(s):  
Ângela de Fátima Barbosa Abreu ◽  
Magno Antonio Patto Ramalho ◽  
Flávia Maria Avelar Gonçalves ◽  
Hélia Alves de Mendonça
Keyword(s):  
Phaseolus Vulgaris ◽  
Minas Gerais ◽  
Colletotrichum Lindemuthianum ◽  
Phaseolus Vulgaris L

O trabalho foi realizado com o objetivo de verificar se as famílias de feijão (Phaseolus vulgaris L.) com maior produtividade de grãos são as que possuem maior resistência ao Colletotrichum lindemuthianum e, assim, poder utilizar a produtividade de grãos como critério auxiliar na seleção de linhagens mais resistentes. Para isso, foram realizados cruzamentos entre as linhagens AN 730340, AN 910522, AN 910523 e AN 910546, resistentes ao patógeno, com a cultivar Carioca, que é suscetível. A partir da geração F2, as populações foram avançadas em dois locais no sul de Minas Gerais, Lavras e Lambari, sendo selecionadas 100 plantas resistentes (sem sintomas) e 100 suscetíveis (com alta severidade da doença), formando, assim, duas subpopulações por cruzamento em cada local. Essas populações foram conduzidas em "bulk" até a geração F5. Cinqüenta plantas F5 deram origem às famílias F5:6 e, posteriormente, 21 famílias F5:7, que foram avaliadas com relação à produtividade de grãos e severidade do patógeno nos dois locais. Constatou-se que, especialmente sob alta severidade da doença, a produtividade de grãos foi um ótimo critério seletivo para identificação de famílias resistentes.

Ultrastructure of tobacco mosaic virus lesions and surrounding tissue in Phaseolus vulgaris var. Pinto

1971 ◽  
Vol 49 (3) ◽  
pp. 417-421 ◽  
Author(s):  
D. F. Spencer ◽  
W. C. Kimmins
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Phaseolus Vulgaris ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Healthy Tissue ◽  
Surrounding Tissue ◽  
Infected Area ◽  
Membrane Bound

Leaves of Phaseolus vulgaris var. Pinto were inoculated with the U1 strain of tobacco mosaic virus TMV (U1) and fully expanded lesions and adjacent healthy tissue were examined in the electron microscope. Emphasis was placed on the band of healthy cells (resistant zone) surrounding the lesion, with the object of detecting the first changes in ultrastructure as healthy tissue graded into the infected area. Cells in the resistant zone were characterized by the appearance of membrane-bound vesicular bodies (paramural bodies) between the plasmalemma and cell wall. Where paramural bodies accumulated, the plasmalemma was withdrawn and intercellular cytoplasmic connections through the plasmodesmata were severed. These changes were found most frequently for a distance of about three cell diameters beyond cells visibly infected at the lesion periphery. It is suggested that these changes in ultrastructure are related to the events of localization. Spread of the virus may be inhibited because of a lack of cytoplasmic connections between cells surrounding the virus-induced lesion.

Phaseolus vulgaris-Colletotrichum lindemuthianum Pathosystem in the Post-Genomic Era: An Update

2022 ◽  
Vol 79 (2) ◽  
Author(s):  
Aasiya Nabi ◽  
Irtifa Lateef ◽  
Qadrul Nisa ◽  
Aqleema Banoo ◽  
Rovidha S. Rasool ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Colletotrichum Lindemuthianum

Characterization of Colletotrichum lindemuthianum Races in Zambia and Evaluation of the CIAT Phaseolus Core Collection for Resistance to Anthracnose

Plant Disease ◽  
2021 ◽  
Author(s):  
Kelvin Kamfwa ◽  
Paul Gepts ◽  
Swivia Hamabwe ◽  
Zombe Kapata Nalupya ◽  
Chikoti Mukuma ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Core Collection ◽  
Durable Resistance ◽  
Colletotrichum Lindemuthianum ◽  
Sources Of Resistance ◽  
Runner Bean ◽  
Causal Pathogen ◽  
Scarlet Runner Bean

Colletotrichum lindemuthianum, the causal pathogen of common bean (Phaseolus vulgaris) anthracnose, is highly variable. Therefore, understanding its race structure and identification of new sources of resistance is necessary for the development of varieties with durable resistance. The objectives of this study were (i) to characterize isolates of C. lindemuthianum collected from three major bean-growing regions in Zambia, and (ii) evaluate the CIAT Phaseolus core collection for resistance to C. lindemuthianum races 37, 73, and 566, and a blend of 20 races. Isolates collected from three major bean-growing districts in Zambia, namely Mporokoso, Mpika, and Mbala, were characterized as race 37, 73, and 566, respectively. A subset of the CIAT core collection comprised of 885 accessions of common bean, 13 accessions of scarlet runner bean (P. coccineus), and 11 accessions of year bean (P. dumosus) were evaluated for resistance to races 37, 73 and 566, and a blend of 20 races in a greenhouse at University of Zambia, Lusaka, Zambia. A total of 72%, 66%, 48% and 9% of P. vulgaris accessions evaluated were highly resistant to races 37, 73, 566 and a blend of 20 races, respectively. Also, accessions of P. coccineus and P. dumosus, highly resistant to races 37, 73 and 566, were identified. Only eight of the 331 P. vulgaris accessions were highly resistant to all three individual races (37, 73, and 566) and to a blend of 20 races. These eight accessions constitute a valuable breeding resource for developing varieties with durable resistance to C. lindemuthianum.

Sign in / Sign up

Export Citation Format

Share Document