The visualization of local lesions caused by the common bean mosaic virus

1972 ◽  
Vol 14 (5) ◽  
pp. 369-370 ◽  
Author(s):  
J. Polák ◽  
J. Chod
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Local Lesions ◽  
The Common

scholarly journals Effect of Temperature and Zinc on Development of Tobacco-Mosaic Virus in Resistant and Susceptible Tomatoes

1969 ◽  
Vol 49 (1) ◽  
pp. 112-132
Author(s):  
Justo López García
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Effect Of Temperature ◽  
Late Winter ◽  
Virus Concentration ◽  
Buffer Solution ◽  
Tomato Plants ◽  
Foliar Sprays ◽  
Local Lesions ◽  
The Common

An investigation of the effects of temperature and of zinc on the development of tobacco-mosaic virus (TMV) in resistant and susceptible varieties of tomatoes was conducted under greenhouse conditions. The study included two experiments, one run during late winter and early spring, and the other during late spring and summer, 1963. Each experiment comprised four treatments and two temperatures. The tomato lines Hawaii 6832, OSU-8, and California 62 PM 22 were used as resistant varieties, whereas the OSU-435-4 line was used as the susceptible one. The common strain of tobacco-mosaic virus was used in this study. Zinc foliar sprays were applied to the tomato plants about 10 days before the seedlings were inoculated with TMV. Immediately after inoculation the plants were divided into two lots and moved to the two temperature houses (cool and hot). The virus concentration was determined by the local lesion assay. One gram of leaf tissue from the tomato plants was ground in a mortar with pestle and a 10-ml. buffer solution added. The extracted sap was squeezed through cheesecloth. One carborundum-dusted leaf of Necrotic Turk tobacco was inoculated. The local lesions were subsequently counted and used as a criterium. Increase in temperature tended to increase virus concentration, although the differences were not significant. Fresh weights of the tomato plants were found to be greater at lower temperatures. Zinc foliar sprays resulted in higher virus concentration and in production of an increased number of local lesions when the inoculum prepared from such treated plants was rubbed into Necrotic Turk tobacco leaves. Effects of zinc on fresh weights were not statistically significant. Zinc applied as foliar sprays appeared to be involved in virus synthesis and multiplication, with higher virus concentrations associated with the addition of zinc. The varieties Hawaii 6832 and OSU-8 were highly resistant, the variety California 62 PM 22 was heterozygous for resistance, and the variety OSU- 435-4 was highly susceptible to the common strain of TMV under the conditions prevailing in these experiments.

scholarly journals Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Evdoxia Efstathiadou ◽  
Georgia Ntatsi ◽  
Dimitrios Savvas ◽  
Anastasia P. Tampakaki
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Phylogenetic Affiliation ◽  
Rhizobial Inoculation ◽  
Rhizobial Species ◽  
The Common ◽  
First Time ◽  
Of Greece ◽  
Rhizobial Isolates

AbstractPhaseolus vulgaris (L.), commonly known as bean or common bean, is considered a promiscuous legume host since it forms nodules with diverse rhizobial species and symbiovars. Most of the common bean nodulating rhizobia are mainly affiliated to the genus Rhizobium, though strains belonging to Ensifer, Pararhizobium, Mesorhizobium, Bradyrhizobium, and Burkholderia have also been reported. This is the first report on the characterization of bean-nodulating rhizobia at the species and symbiovar level in Greece. The goals of this research were to isolate and characterize rhizobia nodulating local common bean genotypes grown in five different edaphoclimatic regions of Greece with no rhizobial inoculation history. The genetic diversity of the rhizobial isolates was assessed by BOX-PCR and the phylogenetic affiliation was assessed by multilocus sequence analysis (MLSA) of housekeeping and symbiosis-related genes. A total of fifty fast-growing rhizobial strains were isolated and representative isolates with distinct BOX-PCR fingerpriniting patterns were subjected to phylogenetic analysis. The strains were closely related to R. anhuiense, R. azibense, R. hidalgonense, R. sophoriradicis, and to a putative new genospecies which is provisionally named as Rhizobium sp. I. Most strains belonged to symbiovar phaseoli carrying the α-, γ-a and γ-b alleles of nodC gene, while some of them belonged to symbiovar gallicum. To the best of our knowledge, it is the first time that strains assigned to R. sophoriradicis and harbored the γ-b allele were found in European soils. All strains were able to re-nodulate their original host, indicating that they are true microsymbionts of common bean.

Inheritance and development of molecular markers linked to angular leaf spot resistance genes in the common bean accession G10909

2010 ◽  
Vol 28 (1) ◽  
pp. 57-71 ◽  
Author(s):  
George S. Mahuku ◽  
María Antonia Henríquez ◽  
Carmenza Montoya ◽  
Carlos Jara ◽  
Henry Teran ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Common Bean ◽  
Resistance Genes ◽  
Leaf Spot ◽  
Angular Leaf Spot ◽  
The Common

scholarly journals Genetic gain in the breeding program of common beans at IAC from 1989 to 2007

2010 ◽  
Vol 10 (4) ◽  
pp. 329-336 ◽  
Author(s):  
Alisson Fernando Chiorato ◽  
Sérgio Augusto Morais Carbonell ◽  
Roland Vencovsky ◽  
Nelson da Silva Fonseca Júnior ◽  
José Baldin Pinheiro
Keyword(s):  
Grain Yield ◽  
Common Bean ◽  
Genetic Gain ◽  
Grain Quality ◽  
The State ◽  
Breeding Program ◽  
Common Beans ◽  
The Common ◽  
The Mean ◽  
Second Period

The goal of the present work was to evaluate the genetic gain obtained in grain yield for the common bean genotypes from 1989 until 2007, at the Instituto Agronômico de Campinas, in the state of São Paulo. Genetic gain has been separated into two research periods; the first, from 1989 to 1996, and the second, from 1997 to 2007. In the first period, a genetic gain of 1.07 % per year was obtained, whereas for the second period, the gain was zero. However, the mean yield of the evaluated lines was approximately 1000 kg ha-1 superior to the figures obtained in the first period. The main cause for the absence of genetic gain in the second period is that the focus of the breeding program was changed to grain quality. The individualized analysis of the genotypes with carioca grains in the second period indicated the lack of genetic gain during the investigated period.

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