Induction of tumor formation in Nicotiana amphiploids with triiodobenzoic acid

1970 ◽  
Vol 48 (12) ◽  
pp. 2209-2212 ◽  
Author(s):  
Ira H. Ames
Keyword(s):  
Nutrient Medium ◽  
Parental Species ◽  
Tumor Formation ◽  
Trigger Mechanism ◽  
Tumor Induction ◽  
Nicotiana Species ◽  
Triiodobenzoic Acid ◽  
Species Hybrids

Young seedlings of two tumorous Nicotiana amphiploids and the parental species, grown aseptically on nutrient medium, were treated with 2,3,5-triiodobenzoic acid. The incidence of tumor formation was scored for 18 days subsequent to exposure. Triiodobenzoic acid markedly accelerated the rate of tumorigenesis in both amphiploids, but it did not induce tumors in the parental species. The compound also induced tumor formation in a small percentage of seedlings of a non-tumorous mutant of the N. glauca × N. langsdorffii amphiploid. These results are discussed in relation to the problem of the trigger mechanism for tumor induction in Nicotiana species hybrids.<

The influence of cytokinins on genetic tumor formation

1972 ◽  
Vol 50 (11) ◽  
pp. 2235-2238 ◽  
Author(s):  
Ira H. Ames
Keyword(s):  
Nutrient Medium ◽  
Tumor Formation ◽  
Trigger Mechanism ◽  
Tumor Induction ◽  
Nicotiana Species ◽  
Species Hybrids

Young seedlings of two tumor-prone Nicotiana amphiploids, grown aseptically on nutrient medium, were treated with either N6-benzyladenine or kinetin. The incidence of tumor formation was scored for 18 days subsequent to exposure. These cytokinins markedly accelerated the rate of tumorigenesis in both amphiploids. The results are discussed in relation to the problem of the trigger mechanism for tumor induction in Nicotiana species hybrids.

Gibberellic acid: effect on genetic tumor induction

1971 ◽  
Vol 49 (9) ◽  
pp. 1699-1701 ◽  
Author(s):  
Ira H. Ames
Keyword(s):  
Gibberellic Acid ◽  
Nutrient Medium ◽  
Stem Growth ◽  
Tumor Formation ◽  
Trigger Mechanism ◽  
Tumor Induction ◽  
Acid Effect ◽  
Nicotiana Suaveolens ◽  
Marked Enhancement

Young seedlings of the amphiploid Nicotiana suaveolens × N. langsdorffii, grown aseptically on nutrient medium, were treated with several concentrations of gibberellic acid (GA3). The incidence of tumor formation was scored for 18 days subsequent to exposure. At a concentration of 5 × 10−3 M, GA3 caused a striking reduction in the rate of tumor formation and a marked enhancement of stem growth in this tumor-prone amphiploid. These results are discussed in relation to the problem of the trigger mechanism for tumor induction in Nicotiana hybrids.

Effects of mercaptoethanol on tumor induction in a Nicotiana amphiploid

1969 ◽  
Vol 47 (6) ◽  
pp. 921-924 ◽  
Author(s):  
I. H. Ames ◽  
H. H. Smith
Keyword(s):  
Nutrient Medium ◽  
Tumor Formation ◽  
Test Solution ◽  
Nicotiana Glauca ◽  
Tumor Induction ◽  
Alkaline Range

Young seedlings of the amphiploid Nicotiana glauca × N. langsdorffii, grown aseptically on nutrient medium, were treated with solutions containing 2-mercaptoethanol, and the incidence of tumor formation was scored for 30 days subsequent to exposure. Mercaptoethanol caused a marked acceleration in the rate of tumorigenesis when the pH of the test solution was in the alkaline range. The effectiveness of the compound was reduced by lowering the pH of the treatment solution.

scholarly journals Patterns of evolution of genes disrupted in expression in Drosophila species hybrids

2005 ◽  
Vol 85 (2) ◽  
pp. 119-125 ◽  
Author(s):  
MOHAMED A. F. NOOR
Keyword(s):  
Amino Acid ◽  
Parental Species ◽  
Species Group ◽  
Pure Species ◽  
X Chromosomes ◽  
Regulatory Pathways ◽  
Regulatory Architecture ◽  
Species Groups ◽  
Male Specific ◽  
Species Hybrids

Divergence between species in regulatory pathways may contribute to hybrid incompatibilities such as sterility. Consistent with this idea, genes involved in male fertility often evolve faster than most other genes both in amino acid sequence and in expression. Previously, we identified a panel of male-specific genes underexpressed in sterile male hybrids of Drosophila simulans and D. mauritiana relative to pure species, and we showed that this underexpression is associated with infertility. In a preliminary effort to assess the generalities in the patterns of evolution of these genes, I examined patterns of mRNA expression in three of these genes in sterile F1 hybrid males of D. pseudoobscura and D. persimilis. F1 hybrid males bearing D. persimilis X chromosomes underexpressed all these genes relative to the parental species, while hybrids bearing D. pseudoobscura X chromosomes underexpressed two of these three genes. Interestingly, the third gene, CG5762, has undergone extensive amino acid evolution within the D. pseudoobscura species group, possibly driven by positive natural selection. We conclude that some of the same genes exhibit disruptions in expression within each of the two species groups, which could suggest commonalities in the regulatory architecture of sterility in these groups. Alternative explanations are also considered.

scholarly journals Evaluation of High-Resolution Melting for Rapid Differentiation of Phytophthora Hybrids and Their Parental Species

Plant Disease ◽  
2019 ◽  
Vol 103 (9) ◽  
pp. 2295-2304
Author(s):  
Maria F. Ratti ◽  
Rhys A. Farrer ◽  
Liliana M. Cano ◽  
Roberto Faedda ◽  
Erica M. Goss
Keyword(s):  
High Resolution ◽  
Genome Sequencing ◽  
High Resolution Melting ◽  
Parental Species ◽  
Phytophthora Species ◽  
Wild Plants ◽  
Whole Genome ◽  
Advance Research ◽  
Species Hybrids ◽  
Rapid Tool

Phytophthora species hybrids have been repeatedly reported as causing damaging diseases to cultivated and wild plants. Two known hybrids, P. andina and P. × pelgrandis, are pathogens of Solanaceae and ornamentals, respectively, although the extent of their host ranges are unknown. P. andina emerged from hybridization of P. infestans and an unidentified related species, whereas P. × pelgrandis emerged from P. nicotianae and P. cactorum. Considering that hybrids and parental species can coexist in the same regions and to distinguish them usually requires cloning or whole genome sequencing, we aimed to develop a rapid tool to distinguish them. Specifically, we used high-resolution melting (HRM) assays to differentiate genotypes based on their amplicon melting profiles. We designed primers for P. × pelgrandis and parental species based on available sequences of P. nicotianae and P. cactorum nuclear genes containing polymorphisms between species. For P. andina, heterozygous sites from Illumina short reads were used for the same purpose. We identified multiple amplicons exhibiting differences in melting curves between parental species and hybrids. We propose HRM as a rapid method for differentiation of P. andina and P. × pelgrandis hybrids from parental species that could be employed to advance research on these pathogens.

Untersuchungen an Crown-Gall

1970 ◽  
Vol 25 (7) ◽  
pp. 735-738 ◽  
Author(s):  
Rolf Beiderbeck
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Tumor Formation ◽  
Tumor Induction ◽  
Host System ◽  
Induced Tumors ◽  
Kalanchoë Daigremontiana ◽  
Kalanchoe Daigremontiana

Concentrations of 10 µg/ml rifampicin reduce the growth of Agrobacterium tumefaciens by more than 90%, concentrations of 100 µg/ml prevent tumor formation in Kalanchoë daigremontiana by the same bacteria.A treatment with rifampicin does not irreversibly alter the bacteria; after removal of the drug bacterial propagation and the ability of tumor induction are restored.0-24 h treatments of the plant wounds with rifampicin prior to infection prevent tumor formation completely.Addition of rifampicin after infection inhibits tumor formation optimally if the drug is added only few hours after inoculation. 30 h after infection the bacteria-host-system is resistant against rifampicin.100 μg/ml rifampicin do not influence the growth of already induced tumors.

HYBRIDIZATION BETWEEN TWO CYPRINID FISHES, HYBOPSIS PLUMBEA AND RHINICHTHYS CATARACTAE, IN ALBERTA

1966 ◽  
Vol 44 (5) ◽  
pp. 963-968 ◽  
Author(s):  
J. S. Nelson
Keyword(s):  
Environmental Changes ◽  
Parental Species ◽  
Rhinichthys Cataractae ◽  
Cyprinid Fishes ◽  
Physical Changes ◽  
Species Hybrids

Five hybrids between Hybopsis plumbea and Rhinichthys cataractae seined from Upper Kananaskis Reservoir, Alberta, were intermediate in most of the characters examined which differ between their parental species. Hybrids of each sex were present. Environmental changes may have been conducive to the hybridization. Marked physical changes occur annually in Upper Kananaskis Reservoir and Hybopsis plumbea and probably Rhinichthys cataractae were introduced.

Auxin–cytokinin interaction in the inhibition, release, and morphology of gametophore buds of Plagiomnium cuspidatum from apical dominance

1981 ◽  
Vol 59 (5) ◽  
pp. 750-762 ◽  
Author(s):  
L. P. Nyman ◽  
E. G. Cutter
Keyword(s):  
Apical Dominance ◽  
Nutrient Medium ◽  
Indoleacetic Acid ◽  
Inhibitory Influence ◽  
Intact Control ◽  
Triiodobenzoic Acid ◽  
Apical Bud ◽  
Lateral Buds ◽  
Activation Response ◽  
Bud Morphology

Observations were made on the effects of indoleacetic acid (IAA), kinetin (K), benzyladenine (BA), and triiodobenzoic acid (TIBA) on the lateral buds of decapitated and intact gametophores of Plagiomnium cuspidatum (Hedw.) Kop. Gametophores were cultured in both solid and liquid Parker's nutrient medium and treated with hormones either in solution or in lanolin paste. Based on numbers of activated buds counted from day 16 to 24, it appeared that IAA, K, and BA, when supplied to the decapitated stump, can to varying degrees replace the inhibitory influence of the apical bud. Closer study, however, of activated and inhibited lateral buds suggests that only IAA in the presence of a cytokinin can maintain inhibition in a morphological and anatomical state which is similar to that of intact control plants. Treatment of intact gametophores with TIBA increased bud activation below the treatment ring. Based on bud morphology and anatomical observations in conjunction with records of bud activation response, endogenous auxins and cytokinins appear to be at suboptimal concentrations for growth at inhibited bud sites.

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