Gibberellic acid: effect on genetic tumor induction

1971 ◽  
Vol 49 (9) ◽  
pp. 1699-1701 ◽  
Author(s):  
Ira H. Ames

Young seedlings of the amphiploid Nicotiana suaveolens × N. langsdorffii, grown aseptically on nutrient medium, were treated with several concentrations of gibberellic acid (GA3). The incidence of tumor formation was scored for 18 days subsequent to exposure. At a concentration of 5 × 10−3 M, GA3 caused a striking reduction in the rate of tumor formation and a marked enhancement of stem growth in this tumor-prone amphiploid. These results are discussed in relation to the problem of the trigger mechanism for tumor induction in Nicotiana hybrids.

1972 ◽  
Vol 50 (11) ◽  
pp. 2235-2238 ◽  
Author(s):  
Ira H. Ames

Young seedlings of two tumor-prone Nicotiana amphiploids, grown aseptically on nutrient medium, were treated with either N6-benzyladenine or kinetin. The incidence of tumor formation was scored for 18 days subsequent to exposure. These cytokinins markedly accelerated the rate of tumorigenesis in both amphiploids. The results are discussed in relation to the problem of the trigger mechanism for tumor induction in Nicotiana species hybrids.


1970 ◽  
Vol 48 (12) ◽  
pp. 2209-2212 ◽  
Author(s):  
Ira H. Ames

Young seedlings of two tumorous Nicotiana amphiploids and the parental species, grown aseptically on nutrient medium, were treated with 2,3,5-triiodobenzoic acid. The incidence of tumor formation was scored for 18 days subsequent to exposure. Triiodobenzoic acid markedly accelerated the rate of tumorigenesis in both amphiploids, but it did not induce tumors in the parental species. The compound also induced tumor formation in a small percentage of seedlings of a non-tumorous mutant of the N. glauca × N. langsdorffii amphiploid. These results are discussed in relation to the problem of the trigger mechanism for tumor induction in Nicotiana species hybrids.<


1969 ◽  
Vol 47 (6) ◽  
pp. 921-924 ◽  
Author(s):  
I. H. Ames ◽  
H. H. Smith

Young seedlings of the amphiploid Nicotiana glauca × N. langsdorffii, grown aseptically on nutrient medium, were treated with solutions containing 2-mercaptoethanol, and the incidence of tumor formation was scored for 30 days subsequent to exposure. Mercaptoethanol caused a marked acceleration in the rate of tumorigenesis when the pH of the test solution was in the alkaline range. The effectiveness of the compound was reduced by lowering the pH of the treatment solution.


1973 ◽  
Vol 51 (3) ◽  
pp. 639-641
Author(s):  
Ira H. Ames

Young seedlings of the tumor-prone amphiploid Nicotiana suaveolens × N. langsdorffii were grown aseptically on nutrient medium under either 2000, 1000, or 500 ft-c of illumination. The incidence of tumor formation was scored at regular intervals beginning 17 days after the seeds had been sown. The rate of tumorigenesis was significantly reduced in those plants that were exposed to either 1000 or 500 ft-c of illumination. These seedlings were also taller and heavier than those grown under 2000 ft-c.


1991 ◽  
Vol 31 (4) ◽  
pp. 243-250 ◽  
Author(s):  
B. Brückner ◽  
D. Blechschmidt ◽  
R.-D. Recknagel

1970 ◽  
Vol 25 (7) ◽  
pp. 735-738 ◽  
Author(s):  
Rolf Beiderbeck

Concentrations of 10 µg/ml rifampicin reduce the growth of Agrobacterium tumefaciens by more than 90%, concentrations of 100 µg/ml prevent tumor formation in Kalanchoë daigremontiana by the same bacteria.A treatment with rifampicin does not irreversibly alter the bacteria; after removal of the drug bacterial propagation and the ability of tumor induction are restored.0-24 h treatments of the plant wounds with rifampicin prior to infection prevent tumor formation completely.Addition of rifampicin after infection inhibits tumor formation optimally if the drug is added only few hours after inoculation. 30 h after infection the bacteria-host-system is resistant against rifampicin.100 μg/ml rifampicin do not influence the growth of already induced tumors.


2013 ◽  
Vol 21 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Małgorzata Zalewska ◽  
Małgorzata Antkowiak

Abstract The research addressed the effect of gibberellic acid (GA3) on growth and flowering of Ajania pacifica /Nakai/ Bremer et Humphries ‘Bea’. The vegetative growth lasted for 8 weeks in glasshouse, under natural long day and from August 15 - under natural short day. The plants were divided into four groups: 1 - the plants sprayed with distilled water (control combination); 2, 3 and 4 - the plants sprayed with GA3 ones, twice and thrice, respectively, starting from the 5th week of growth, and then in the 6th and 7th week, successively. Each of the group was divided into two subgroups: the first one treated with GA3 at concentration of 250 mg·dm-3 and the second with 500 mg·dm-3. It was observed that double spraying with GA3 accelerated the buds development of Ajania pacifica, thus shortened the cultivation time by about two days. Treatment with GA3 at concentration 500 mg·dm-3 stimulated the elongation of shoots more than treatment with GA3 at 250 mg·dm-3. The plants sprayed twice were longer than the control and then those sprayed one time. However, elongation of shoots was not remarkable; corymbs were shorter, narrower, and contained lesser number of inflorescences. The present study indicates the specificity of the response of Ajania pacifica to GA3 treatment, which appears to be different from other ornamental species.


2016 ◽  
Vol 69 (1) ◽  
Author(s):  
Marian Saniewski ◽  
Justyna Góraj-Koniarska ◽  
Elżbieta Węgrzynowicz-Lesiak ◽  
Eleonora Gabryszewska

It is known that chilling of <em>Muscari</em> bulbs is necessary for the growth of the inflorescence stalk and flowering, but not for the growth of leaves. Gibberellic acid (GA) accelerated stem growth and flowering in chilled <em>Muscari</em> bulbs. In the present experiment it was shown that in unchilled derooted <em>Muscari</em> bulbs the growth of leaves, but not the growth of the inflorescence stalk, was observed when bulbs were stored in water, GA at a concentration of 50 and 100 mg/L, benzyladenine (BA) at a concentration of 25 and 50 mg/L, or a mixture of GA+BA (50+25 mg/L), but abscisic acid (ABA) at a concentration of 10 mg/L greatly inhibited the growth of leaves. In chilled derooted <em>Muscari</em> bulbs the growth of leaves and inflorescence stalk was observed when bulbs were stored in water or GA, but BA and GA+BA treatments totally inhibited the growth of the inflorescence stalk without an effect on the growth of leaves. These results clearly showed that the growth of leaves and inflorescence stalk in <em>Muscari</em> bulbs are controlled by plant growth regulators in different ways. ABA totally inhibited the growth of leaves and inflorescence stalk in chilled derooted <em>Muscari</em> bulbs. It was shown that after the excision of the inflorescence bud in cultivated chilled <em>Muscari</em> bulbs, the inflorescence stalk died, but application of indole-3-acetic acid (IAA) 0.5% in the place of the removed inflorescence bud induced the growth of the inflorescence stalk. IAA applied under the inflorescence bud inhibited the development of flowers (flower-bud blasting) and induced the growth of the inflorescence stalk below the treatment site. These results are discussed with reference to hormonal regulation of stem (stalk) growth in tulip, narcissus, hyacinth, and <em>Hippeastrum</em>.


2004 ◽  
pp. 447-452
Author(s):  
E. Sánchez-Sánchez ◽  
F. Cabrera-Carbajal ◽  
I. Padilla-Valenzuela ◽  
J.A. Samaniego Russo ◽  
R. Aboytia-Mendivil

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