THE UPTAKE AND DISTRIBUTION OF 32P BY BEAN SEEDLINGS (PHASEOLUS VULGARIS) GROWING AT TWO PHOSPHATE LEVELS, AND SOME EFFECTS OF 2,4-D

1967 ◽  
Vol 45 (7) ◽  
pp. 1011-1017 ◽  
Author(s):  
Harold M. Etter
Keyword(s):  
Phaseolus Vulgaris ◽  
Distribution Pattern ◽  
Organic Phosphorus ◽  
Phosphorus Fractions ◽  
Dichlorophenoxyacetic Acid ◽  
Free Solution ◽  
Leaf Tissues ◽  
2,4 Dichlorophenoxyacetic Acid

The uptake and subsequent distribution of 32P among the root, stem, and leaf tissues of bean seedlings growing in complete or phosphate-free nutrient and its incorporation into organic phosphorus fractions was studied. Plants growing in complete nutrient took up, retained, translocated, and incorporated less activity than those growing in phosphate-free solution. These results are discussed in relation to phosphate pools which appear to be present in bean seedlings. Fifty and 70 h after spraying with 5 p.p.m. 2,4-dichlorophenoxyacetic acid (2,4-D), no marked changes were observed in the uptake or distribution pattern of the 32P as compared to controls.

EXPERIMENTS ON THE RELATIVE SUSCEPTIBILITY OF WEEDS TO AUXIN HERBICIDES

1962 ◽  
Vol 40 (11) ◽  
pp. 1401-1409 ◽  
Author(s):  
J. R. Hay
Keyword(s):  
Phaseolus Vulgaris ◽  
Root Formation ◽  
Effective Control ◽  
Inorganic Salts ◽  
Dichlorophenoxyacetic Acid ◽  
Functional Importance ◽  
Molecular Configuration ◽  
Auxin Herbicides ◽  
Chlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

Sections of stems of Silene cucubalus, Galeopsis tetrahit, and Phaseolus vulgaris were cultured under sterile conditions in media containing inorganic salts, sucrose, agar, and the herbicides 2-methyl, 4-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid, or 2-(2,4,5-trichlorophenoxy)propionic acid. The herbicide which gave the most effective control of a species under field conditions was also the most active in inducing root formation and proliferation on stem sections of that species. The response of the sections to these auxin herbicides diminished as the plants from which the sections were taken became more mature. The amount of herbicide recovered from treated shoots of these species was not correlated with susceptibility. It is postulated that susceptibility to these materials depends upon (i) the affinity of the species for the molecular configuration of the herbicide and (ii) the relative amount and functional importance of the susceptible tissues at the time of treatment.

Metabolic Fate of 2,4-Dichlorophenoxyacetic Acid in the Stem Tissue of Phaseolus vulgaris

Nature ◽  
1964 ◽  
Vol 204 (4961) ◽  
pp. 884-885 ◽  
Author(s):  
E. W. THOMAS ◽  
B. C. LOUGHMAN ◽  
R. G. POWELL
Keyword(s):  
Phaseolus Vulgaris ◽  
Dichlorophenoxyacetic Acid ◽  
Stem Tissue ◽  
Metabolic Fate ◽  
2,4 Dichlorophenoxyacetic Acid

In vitro culture of Bouvardia ternifolia

1982 ◽  
Vol 60 (6) ◽  
pp. 917-921 ◽  
Author(s):  
Leonor Fernandez ◽  
Estela Sanchez de Jimenez
Keyword(s):  
Indoleacetic Acid ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Napthaleneacetic Acid ◽  
Leaf Tissues ◽  
2,4 Dichlorophenoxyacetic Acid

Callus cultures were induced from radicle and leaf tissues of Bouvardia ternifolia (trompetilla). Optimum growth regulator concentrations for radicle callus cultures were 1 mg/L 2,4-dichlorophenoxyacetic acid and 0.005 mg/L kinetin; for leaf callus they were either 2 mg/L naphthaleneacetic acid and 0.002 mg/L benzylaminopurine or 5 mg/L of idoleacetic acid and 0.01 mg/L kinetin. Callus has been maintained in culture for nearly 3 years with a very rapid growth rate.A generation time of approximately 24 to 28 h was obtained for batch cell suspension cultures. Production of protoplasts from suspension cultures was optimized with a yield of 70 to 90%. Protoplast culture was achieved in droplets of fresh medium with 2 mg/L napthaleneacetic acid, 0.01 mg/L benzylaminopurine, and0.5 M mannitol. After 2 years, callus in culture still retained its organogenetic capacity. An average of 18 complete plantlets from approximately 2 g of callus can be obtained after transfer to medium with 0.1 mg/L indoleacetic acid and 0.1 mg/L benzylaminopurine.

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