EFFECT OF PLANT GROWTH REGULATORS ON THE INFECTIVITY OF TOBACCO MOSAIC VIRUS

1964 ◽  
Vol 42 (7) ◽  
pp. 885-889 ◽  
Author(s):  
Victor Weeraratne ◽  
Avery E. Rich
Keyword(s):  
Growth Regulators ◽  
Room Temperature ◽  
Indoleacetic Acid ◽  
Inhibitory Effect ◽  
Indolebutyric Acid ◽  
Meat Extract ◽  
Naturally Occurring ◽  
Chlorophenoxyacetic Acid ◽  
Coconut Meat

A naturally occurring growth regulator, coconut meat extract, was tested for the inhibition of tobacco mosaic (TMV) virus infection. Coconut meat extract inhibited infection by 97%. The inhibitor in coconut meat extract was effective when the virus inoculation took place 8 days after the application of the inhibitor, but the inhibitor was ineffective when applied after inoculation with the virus. Aging in vitro for 64 days at 4 °C and at room temperature did not reduce the inhibitory effect of coconut meat extract, but dilution markedly reduced its inhibitory effect. Three commercially available growth regulators, indoleacetic acid, indolebutyric acid, and p-chlorophenoxyacetic acid, were tested at a concentration of 100 p.p.m., but their inhibiting activity was very low.

scholarly journals Growth regulator effect on in vitro regeneration of rhododendron cultivars

2008 ◽  
Vol 35 (No. 2) ◽  
pp. 90-94 ◽  
Author(s):  
H. Vejsadová
Keyword(s):  
Growth Regulators ◽  
Growth Regulator ◽  
Indoleacetic Acid ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Commercial Preparation ◽  
High Level

In Rhododendron L. cv. Azuro, Bohumil Kavka, Catharine van Toll, Grandiflorum, Mars, Nova Zembla, Ortrud, Ovation, Prof. Scholz, Purple Splendour, Rebe and Van Werden Poelman, the effect of growth regulators on organogenesis induction of shoot-tip meristems was tested. All cultivars significantly showed the highest shoot regeneration on MS medium containing 6 mg/dm<sup>3</sup> isopentenyladenine (2iP). For most rhododendrons, the highest shoot multiplication was found on a medium with 8&ndash;10 mg/dm<sup>3</sup> 2iP in combination with 1 mg/dm<sup>3</sup> indoleacetic acid (IAA). Shoots rooted successfully in the substrate with high level of peat without growth regulators. However, the commercial preparation Racine significantly increased rooting in cv. Grandiflorum, Nova Zembla and Rebe compared with 0.03% indolebutyric acid (IBA).

scholarly journals α-1-Anti-trypsin, an inhibitor of renin

1976 ◽  
Vol 153 (2) ◽  
pp. 505-507 ◽  
Author(s):  
S Scharpé ◽  
M Eid ◽  
W Cooreman ◽  
A Lauwers
Keyword(s):  
Human Plasma ◽  
Competitive Inhibition ◽  
Proteinase Inhibitors ◽  
Competitive Inhibitor ◽  
Inhibitory Effect ◽  
Renin Activity ◽  
Pig Kidney ◽  
Naturally Occurring ◽  
The Hill

A naturally occurring competitive inhibitor of pig kidney renin has been identified in human plasma. The inhibitor was shown to be α-1 anti-trypsin and the effect in vitro on the renin activity was examined. The slope in the Hill plot is compatible with the assumption of one-site competitive inhibition. Other proteinase inhibitors, such as α-2-macroglobulin and C1 inactivator, however, have no inhibitory effect on the renin-angiotensinogen reaction.

In Vitro Stabilization of Blood Glucose with Water

1963 ◽  
Vol 9 (5) ◽  
pp. 594-599 ◽  
Author(s):  
Samuel Meites ◽  
Nancy Bohman
Keyword(s):  
Blood Glucose ◽  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Room Temperature ◽  
Inhibitory Effect ◽  
Protein Precipitation ◽  
Shed Blood ◽  
Effect Of Water

Abstract The loss in glucose that shed blood undergoes on standing may be effectively inhibited for 2 hr., prior to protein precipitation, by a dilution with water of 1:80 (v/v). The inhibitory effect of water is independent of glucose concentration and temperature (up to and including room temperature). Incorporation of the dilution into the glucose oxidase method of Washko and Rice (4) makes the method particularly appropriate for the newborn.

scholarly journals TAMARIND EXTRACT INHIBITS CYTOCHROME P450 (CYP3A4 ISOZYME) - AN IN VITRO STUDY

2018 ◽  
Vol 11 (7) ◽  
pp. 333
Author(s):  
Jagadish Rajkumaar R ◽  
Anitha Roy ◽  
Lakshmi T
Keyword(s):  
Room Temperature ◽  
Potassium Phosphate ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Dependent Manner ◽  
Fruit Pulp ◽  
Ic50 Value ◽  
Dose Dependent ◽  
Cytochrome P 450

Objective: The aim of the present study was to analyze the effect of the aqueous fruit pulp extract of Tamarindus indica L. (tamarind extract) on cytochrome P 450 isoform CYP3A4.Methods: Tamarind extract at different concentrations from 5 to 100 μg/ml was examined for its inhibitory property toward cytochrome P 450 isoform CYP3A4. The various concentrations of tamarind extract, potassium phosphate buffer, CYP450 reagent, and substrate 7-Benzyloxy-4- trifluoromethylcoumarin were added to a 96-well plate. The mixtures were preincubated for 20 min at room temperature. The reaction was started by a mixture of free constituted substrate and NADP+ and incubated at room temperature for 30–60 min. The reaction was stopped by Tris-HCl buffer, pH 10.5. The fluorescent intensities of the products were measured by PerkinElmer Enspire fluorescence reader using an excitation and emission wavelength of 405 nm and 460 nm, respectively. Inhibitory concentration (IC50) was calculated by plotting concentrations of tamarind extract against the corresponding percentage inhibition.Results: All the tested concentrations of extract except 5 μg/ml showed good inhibition against CYP3A4 in a dose-dependent manner. The IC50 value of tamarind for CYP3A4 inhibitory activity was found to be 27.89 μg/ml.Conclusion: T. indica aqueous fruit pulp extract exhibited an inhibitory effect on CYP34A, thereby indicating the possibilities of herb-drug interaction if these extracts are coadministered with the prescribed drugs that are metabolized by CYP3A4.

scholarly journals Inhibition of isoleucyl-transfer ribonucleic acid synthetase in Echerichia coli by pseudomonic acid

1978 ◽  
Vol 176 (1) ◽  
pp. 305-318 ◽  
Author(s):  
Julia Hughes ◽  
Graham Mellows
Keyword(s):  
Rna Synthesis ◽  
Inhibitory Effect ◽  
Trna Synthetase ◽  
Threonine Deaminase ◽  
Trna Synthetases ◽  
E Coli ◽  
Naturally Occurring ◽  
Pseudomonic Acid

The mode of action of the antibiotic pseudomonic acid has been studied in Escherichia coli. Pseudomonic acid strongly inhibits protein and RNA synthesis in vivo. The antibiotic had no effect on highly purified DNA-dependent RNA polymerase and showed only a weak inhibitory effect on a poly(U)-directed polyphenylalanine-forming ribosomal preparation. Chloramphenicol reversed inhibition of RNA synthesis in vivo. Pseudomonic acid had little effect on RNA synthesis in a regulatory mutant, E. coli B AS19 RCrel, whereas protein synthesis was strongly inhibited. In pseudomonic acid-treated cells, increased concentrations of ppGpp, pppGpp and ATP were observed, but the GTP pool size decreased, suggesting that inhibition of RNA synthesis is a consequence of the stringent control mechanism imposed by pseudomonic acid-induced deprivation of an amino acid. Of the 20 common amino acids, only isoleucine reversed the inhibitory effect in vivo. The antibiotic was found to be a powerful inhibitor of isoleucyl-tRNA synthetase both in vivo and in vitro. Of seven other tRNA synthetases assayed, only a weak inhibitory effect on phenylalanyl-tRNA synthetase was observed; this presumably accounted for the weak effect on polyphenylalanine formation in a ribosomal preparation. Pseudomonic acid also significantly de-repressed threonine deaminase and transaminase B activity, but not dihydroxyacid dehydratase (isoleucine-biosynthetic enzymes) by decreasing the supply of aminoacylated tRNAIle. Pseudomonic acid is the second naturally occurring inhibitor of bacterial isoleucyl-tRNA synthetase to be discovered, furanomycin being the first.

Potent inhibitory effect of naturally occurring flavonoids quercetin and kaempferol on in vitro osteoclastic bone resorption

2003 ◽  
Vol 65 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Alice Wattel ◽  
Said Kamel ◽  
Romuald Mentaverri ◽  
Florence Lorget ◽  
Christophe Prouillet ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Inhibitory Effect ◽  
Osteoclastic Bone Resorption ◽  
Naturally Occurring ◽  
Potent Inhibitory Effect

REGENERATION OF PLANTLETS FROM HYPOCOTYL DERIVED CALLUS OF MORUS ALBA

1995 ◽  
Vol 43 (3) ◽  
pp. 259-262 ◽  
Author(s):  
K. Kathiravan ◽  
A. Shajahan ◽  
A. Ganapathi
Keyword(s):  
Indoleacetic Acid ◽  
Adventitious Shoot ◽  
Morus Alba ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Shoot Buds ◽  
Adventitious Shoot Buds ◽  
Hypocotyl Segments

Plantlets were regenerated from hypocotyl callus of Morus alba cv. MR2. Calli were established from hypocotyl segments on Murashige and Skoog (MS) medium supplemented with indoleacetic acid (0.5 mg/1) and benzyladenine (BA) (0.5 mg/1). They were transferred to MS medium with different concentrations of naphthaleneacetic acid NAA and BA for four weeks. Adventitious shoot buds were observed by transferring callus onto fresh Linsmaier and Skoog (LS) medium containing NAA (0.5 mg/1) and BA (0.75 mg/1). Shoots produced in vitro were rooted on MS medium with indolebutyric acid (0.75 mg/1).

scholarly journals Propagación in vitro de especies de Echinocereus, Escontria, Mammillaria, Melocactus y Polaskia (Cactaceae)

2017 ◽  
pp. 9
Author(s):  
José Luis Retes-Pruneda ◽  
María de Lourdes Valadez-Aguilar ◽  
Martha Evelia Pérez-Reyes ◽  
Eugenio Pérez-Molphe-Balch
Keyword(s):  
Activated Charcoal ◽  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Basal Medium ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Indolebutyric Acid ◽  
Multiple Shoot Formation ◽  
Escontria Chiotilla

In vitro propagation systems by means of areole activation were developed for Echinocereus knippelianus, Echinocereus schmollii, Mammillaria carmenae, M. carmenae fo. rubrisprina, M. herrerae, M. theresae, Melocactus curvispinus, Escontria chiotilla and Polaskia chichipe. In vitro germinated seedlings were used as source of explants. Multiple shoot formation from areoles was achieved on MS basal medium supplemented with 3% sucrose, 10 g L-1 agar and 6-benzylaminopurine (BA) or 6-(, -dimethylallylamino)purine (2iP). Efficiencies ranged from 6.0 shoots per explant in M. carmenae fo. rubrisprina to 13.5 shoots per explant in Echinocereus schmollii. Rooting of the in vitro generated shoots was achieved in MS basal medium, or MS basal medium supplemented with indoleacetic acid, indolebutyric acid or activated charcoal. Finally, 49-98% of these plants survived.

scholarly journals In vitro antibacterial activity of Spirulina platensis extracts against clinical isolates of Salmonella enterica serovars Typhi and Paratyphi (SUBP03)

2016 ◽  
Vol 5 (1) ◽  
pp. 22-25 ◽  
Author(s):  
Sunjukta Ahsan ◽  
Md Shamsul Arefin ◽  
John Litol Munshi ◽  
Mst Nadira Begum ◽  
Maisha Maliha ◽  
...  
Keyword(s):  
Spirulina Platensis ◽  
Salmonella Enterica ◽  
Test Organism ◽  
Antimicrobial Effect ◽  
Inhibitory Effect ◽  
Clinical Isolates ◽  
Naturally Occurring ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity

Natural therapy has its inherent attraction in that there are limited side-effects. Spirulina platensis, a naturally occurring algae, has been reported to have more than one therapeutic advantage. It is also used as a source of natural protein and vitamins in many parts of the world. In the present investigation, the antimicrobial activity of S. platensis against clinical isolates of Salmonella enterica serovar Typhi (n=17) and Paratyphi (n=3) was investigated. Ethanolic and chloroform extracts of S. platensis (40 mg/mL) were investigated for antimicrobial effect. Variable zones of inhibition were observed for the former extract whereas the latter did not show any inhibitory effect on growth of the test organism. Diameters of the zones of inhibition were 9.7-14.0 mm for S. Paratyphi and 9.5-16.0 mm for S. Typhi. The presence of zones of inhibition at the concentration of 40 mg/ml of the extract used is promising towards developing a natural remedy against infections with S. enterica serovars Typhi and Paratyphi.Stamford Journal of Microbiology, Vol.5(1) 2015: 22-25

Interactions of growth regulators, explant age, and culture environment controlling organogenesis from lettuce cotyledons in vitro

1984 ◽  
Vol 62 (3) ◽  
pp. 586-590 ◽  
Author(s):  
David T. Webb ◽  
Luis D. Torres ◽  
Pierre Fobert
Keyword(s):  
Growth Regulators ◽  
Indoleacetic Acid ◽  
Low Frequency ◽  
Shoot Initiation ◽  
Lactuca Sativa L ◽  
Grand Rapids ◽  
Environment Control ◽  
Culture Environment ◽  
Bud Initiation

Organogenesis in cultured Lactuca sativa L. cv. Grand Rapids cotyledons was controlled by growth regulators and influenced by explant age as well as culture environment. Control explants formed roots with a low frequency. Exposure to indoleacetic acid (IAA) greatly stimulated root initiation. Kinetin (K) applied singly or in combination with IAA induced bud formation. Benzylaminopurine (BA) also caused bud initiation. Root and shoot initiation was optimal with 4-day-old explants. Organogenesis declined with older explants. More buds formed in light than in darkness. Other environmental variables tested had no marked effect on K-induced bud initiation. Control explants on medium containing no growth regulator never formed buds.

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